FastQCFastQC Report
Sat 18 Jun 2016
SRR3553752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201310
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG88990.7407746543356836No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTA65080.5417419317245341No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCG56260.46832208172744755No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47610.3963173535557017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30690.25547111070414796No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT25680.2137666380867553No Hit
GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGT22460.18696256586559673No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC16560.13784951428024406No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT15270.12711123689971782No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT200.00125579140.00000421
GTCGACT200.00125579140.00000439
CTACGCG600.040.0000041
TTGCGGT200.00125579140.00000412
ATACGAG304.8676975E-640.0000041
TGTAACG200.00125579140.0000041
GTACGAG304.8676975E-640.0000041
AACGCGT257.794865E-540.040
TAATGCG257.794865E-540.01
CTTACCG257.794865E-540.01
TACGGGA3450.037.1014484
CACGACC4700.036.59574527
CGTATGG1100.036.3636362
ACTACGG1550.036.1290322
CGTCATA503.425157E-936.038
CTAAACG455.44278E-835.5555531
AAACACG4850.035.46391740
GCCGATA850.035.294129
ACGACCA4900.035.1020428
CGAACCC800.035.00000434