##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553752_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1201310
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.784473616302204	31.0	31.0	34.0	30.0	34.0
2	31.93087129883211	33.0	31.0	34.0	30.0	34.0
3	31.90067842605156	33.0	31.0	34.0	30.0	34.0
4	35.64741157569653	37.0	35.0	37.0	33.0	37.0
5	35.432780048447114	37.0	35.0	37.0	33.0	37.0
6	35.435886657066035	37.0	35.0	37.0	33.0	37.0
7	35.78446778932998	37.0	35.0	37.0	35.0	37.0
8	35.54090950712139	37.0	35.0	37.0	33.0	37.0
9	37.35236200481141	39.0	37.0	39.0	34.0	39.0
10	36.73416353813753	39.0	37.0	39.0	32.0	39.0
11	36.69081502692894	39.0	35.0	39.0	32.0	39.0
12	36.71316396267408	39.0	35.0	39.0	32.0	39.0
13	34.9887914027187	39.0	35.0	39.0	30.0	39.0
14	36.812028535515395	39.0	36.0	41.0	30.0	41.0
15	37.40873962590838	39.0	36.0	41.0	32.0	41.0
16	37.65747975127153	39.0	36.0	41.0	32.0	41.0
17	37.6572075484263	39.0	36.0	41.0	32.0	41.0
18	37.71510101472559	39.0	36.0	41.0	33.0	41.0
19	37.71721537321757	39.0	36.0	41.0	33.0	41.0
20	37.60119702657932	39.0	36.0	41.0	32.0	41.0
21	37.562441834330855	39.0	36.0	41.0	32.0	41.0
22	37.579355037417486	39.0	36.0	41.0	33.0	41.0
23	37.485488341893436	39.0	36.0	41.0	32.0	41.0
24	37.45458957304942	39.0	36.0	41.0	32.0	41.0
25	37.30159492553962	39.0	35.0	41.0	32.0	41.0
26	37.236933014792186	39.0	35.0	41.0	32.0	41.0
27	37.187119061690986	39.0	35.0	41.0	32.0	41.0
28	37.09378095578993	39.0	35.0	41.0	31.0	41.0
29	37.01875452630878	39.0	35.0	41.0	31.0	41.0
30	36.900893191599174	39.0	35.0	41.0	31.0	41.0
31	36.82605489007833	39.0	35.0	41.0	31.0	41.0
32	36.629257227526615	39.0	35.0	40.0	30.0	41.0
33	36.56339745777526	39.0	35.0	40.0	30.0	41.0
34	36.442166468272134	39.0	35.0	40.0	30.0	41.0
35	36.35793175783103	39.0	35.0	40.0	30.0	41.0
36	36.272892925223296	39.0	35.0	40.0	30.0	41.0
37	36.21698062947949	39.0	35.0	40.0	30.0	41.0
38	36.11239480234078	39.0	35.0	40.0	30.0	41.0
39	36.032810848157425	39.0	35.0	40.0	29.0	41.0
40	35.96683870108465	38.0	35.0	40.0	29.0	41.0
41	35.90162988737295	38.0	35.0	40.0	29.0	41.0
42	35.86425651996571	38.0	35.0	40.0	29.0	41.0
43	35.82489449018155	38.0	35.0	40.0	29.0	41.0
44	35.73705538120885	38.0	35.0	40.0	28.0	41.0
45	35.67808975202071	38.0	35.0	40.0	28.0	41.0
46	34.71981586767779	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	29.0
10	39.0
11	27.0
12	14.0
13	22.0
14	17.0
15	24.0
16	44.0
17	122.0
18	222.0
19	502.0
20	927.0
21	1772.0
22	2876.0
23	4274.0
24	6065.0
25	7758.0
26	10024.0
27	12776.0
28	16060.0
29	19544.0
30	24656.0
31	31340.0
32	40732.0
33	54289.0
34	79306.0
35	100777.0
36	117764.0
37	166305.0
38	245465.0
39	257536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.364144142644285	20.015066885316863	19.6195819563643	25.001207015674552
2	22.696889229258062	30.13360414880422	23.498014667321506	23.67149195461621
3	26.86433976242602	29.867394760719545	22.688814710607584	20.579450766246847
4	24.19092490697655	27.06562003146565	24.943519990676844	23.799935070880952
5	23.492437422480457	31.291257044393205	20.29983934205159	24.916466191074743
6	19.68326243850463	41.29084083209163	21.31031956780515	17.71557716159859
7	82.71270529671774	4.873013626790754	9.147680448843346	3.2666006276481507
8	82.40645628522196	6.41582938625334	7.265152208838685	3.91256211968601
9	76.0040289350792	6.869833764806753	9.213358750031215	7.912778550082826
10	34.64909140854567	28.451523753236053	18.264228217529197	18.63515662068908
11	28.4659246988704	26.97455277988196	24.51715210894773	20.042370412299906
12	22.432844145141555	24.661910747434053	30.641965853942775	22.263279253481617
13	21.857139289608842	27.89080254055989	31.72145407929677	18.5306040905345
14	21.57910947215956	28.717982868701668	28.95930276115241	20.743604897986366
15	20.271120693243212	26.424153632284757	32.981495201072164	20.32323047339987
16	22.046599129283866	26.132555293804266	29.026978881387816	22.793866695524052
17	21.489707069782156	26.78284539377846	28.682355095687207	23.045092440752178
18	22.682488283623712	25.02309978273718	29.381175550024558	22.913236383614553
19	23.69113717525035	27.128801058844093	26.68969708068692	22.490364685218637
20	25.917789746193737	25.551522920811447	28.158843262771477	20.37184407022334
21	24.989802798611514	28.299939233003972	27.100415379876967	19.609842588507544
22	23.69171987247255	23.663334193505424	28.17440960285022	24.470536331171804
23	22.15781105626358	27.743713113184775	27.828703665165527	22.26977216538612
24	21.945043327700596	26.1833331945959	29.41056013851545	22.461063339188055
25	22.03802515587151	29.82111195278488	26.54077631918489	21.600086572158727
26	21.201604914634856	27.662218744537213	27.951652779049535	23.18452356177839
27	22.8006093348095	26.728404824732998	26.945834131073575	23.525151709383923
28	20.248811713879014	27.740300172311894	29.680099225012697	22.330788888796395
29	21.701642373741997	27.375281983834316	27.3846051393895	23.538470503034187
30	21.291506771774145	28.08542341277439	27.656142044934278	22.966927770517184
31	22.488616593552038	28.02865205484013	25.064055073211744	24.418676278396084
32	22.568029900691744	28.81595924449143	26.150618907692436	22.465391947124388
33	21.29899859320242	26.978798145357985	26.36263745411259	25.359565807327
34	20.501702308313423	28.156678958803305	27.113151476305035	24.228467256578234
35	20.814444231713715	26.87299697829869	26.371544397366208	25.941014392621387
36	20.942804105518142	29.618499804380217	25.469029642640116	23.96966644746152
37	20.534333352756573	28.43304392704631	26.212967510467738	24.81965520972938
38	20.300422039273794	28.723893083383974	26.526125646169596	24.44955923117264
39	21.913494435241528	26.953991892184366	26.06754293229891	25.0649707402752
40	21.89210112294079	25.42083225811822	28.416561919904105	24.270504699036884
41	19.43145399605431	26.430147089427376	27.02999225845119	27.108406656067125
42	21.023632534483188	26.8389508120302	27.280219094155544	24.857197559331063
43	21.475639093989063	25.946341910081493	27.056879573132665	25.52113942279678
44	21.530579117796407	26.46452622553712	27.46293629454512	24.541958362121353
45	21.447669627323503	25.533875519224846	27.179911929476987	25.83854292397466
46	23.069898693925797	26.592969341801865	25.99595441642873	24.341177547843603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3458.0
1	3588.5
2	3719.0
3	6364.0
4	9009.0
5	6266.5
6	3524.0
7	3524.0
8	3355.5
9	3187.0
10	3155.0
11	3123.0
12	3106.0
13	3089.0
14	3003.0
15	2917.0
16	2889.0
17	2861.0
18	3209.0
19	3557.0
20	3557.0
21	3979.5
22	4402.0
23	4622.0
24	4842.0
25	6168.0
26	7494.0
27	9479.5
28	11465.0
29	14107.0
30	16749.0
31	16749.0
32	19587.0
33	22425.0
34	26559.0
35	30693.0
36	34306.0
37	37919.0
38	43029.5
39	48140.0
40	54974.0
41	61808.0
42	72495.0
43	83182.0
44	83182.0
45	89264.0
46	95346.0
47	111291.0
48	127236.0
49	121845.5
50	116455.0
51	108629.5
52	100804.0
53	92469.0
54	84134.0
55	77575.5
56	71017.0
57	71017.0
58	66133.5
59	61250.0
60	54524.0
61	47798.0
62	43029.5
63	38261.0
64	33583.5
65	28906.0
66	25068.5
67	21231.0
68	17880.0
69	14529.0
70	14529.0
71	12461.0
72	10393.0
73	8427.0
74	6461.0
75	5287.0
76	4113.0
77	3443.5
78	2774.0
79	2303.0
80	1832.0
81	1832.0
82	1311.5
83	791.0
84	492.0
85	193.0
86	158.5
87	124.0
88	82.5
89	41.0
90	36.0
91	31.0
92	27.5
93	24.0
94	24.0
95	12.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1201310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.362839101205346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.83101402046127	22.417191993821017
2	9.875260546972797	5.996818941404324
3	3.639947894508979	3.315574567732419
4	1.8893404295691043	2.294629578816356
5	1.2177966247785255	1.8487881488070654
6	0.8996628038970818	1.638979017603981
7	0.7186560653300963	1.5274306936506121
8	0.5997245355541412	1.4567471662460394
9	0.48428428171164406	1.3233821152368106
>10	6.350500740992719	43.775200337466316
>50	0.44761186827912725	8.371783487860853
>100	0.040221340096915706	2.1534152512412166
>500	0.0024458923012944515	0.47652883741976526
>1k	0.0027176581125493903	1.6679747046931834
>5k	8.15297433764817E-4	1.7355551579999864
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG	8899	0.7407746543356836	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTA	6508	0.5417419317245341	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCG	5626	0.46832208172744755	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4761	0.3963173535557017	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3069	0.25547111070414796	No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	2568	0.2137666380867553	No Hit
GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGT	2246	0.18696256586559673	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC	1656	0.13784951428024406	No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	1527	0.12711123689971782	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09614504166285139	0.0
2	0.0	0.0	0.0	0.5369971114866271	0.0
3	0.0	0.0	0.0	0.702316637670543	0.0
4	0.0	0.0	0.0	1.0684169781322057	0.0
5	0.0	0.0	0.0	2.3595907800650955	0.0
6	0.0	0.0	0.0	3.2324712189193465	0.0
7	0.0	0.0	0.0	3.877350558973121	0.0
8	0.0	0.0	0.0	4.814743904570843	0.0
9	0.0	0.0	0.0	5.21896929185639	0.0
10	0.0	0.0	0.0	6.510975518392422	0.0
11	0.0	0.0	0.0	7.317761443757232	0.0
12	0.0	0.0	0.0	8.603191515928446	0.0
13	0.0	0.0	0.0	9.009914177023417	0.0
14	0.0	0.0	0.0	9.204202079396659	0.0
15	0.0	0.0	0.0	9.509119211527416	0.0
16	0.0	0.0	0.0	10.011487459523353	0.0
17	0.0	0.0	0.0	10.57770267458025	0.0
18	0.0	0.0	0.0	11.245806661061675	0.0
19	0.0	0.0	0.0	11.65244607969633	0.0
20	8.324246031415704E-5	0.0	0.0	12.016382116189826	0.0
21	8.324246031415704E-5	0.0	0.0	12.411367590380502	0.0
22	8.324246031415704E-5	0.0	0.0	12.830410135601968	0.0
23	8.324246031415704E-5	0.0	0.0	13.277338905028676	0.0
24	8.324246031415704E-5	0.0	0.0	13.631702058586043	0.0
25	8.324246031415704E-5	0.0	0.0	13.939782404208739	0.0
26	8.324246031415704E-5	0.0	0.0	14.219310585943678	0.0
27	8.324246031415704E-5	0.0	0.0	14.537962724026272	0.0
28	8.324246031415704E-5	0.0	0.0	14.852535981553471	0.0
29	8.324246031415704E-5	0.0	0.0	15.19174900733366	0.0
30	8.324246031415704E-5	0.0	0.0	15.604881337872822	0.0
31	8.324246031415704E-5	0.0	0.0	15.940181968018246	0.0
32	8.324246031415704E-5	0.0	0.0	16.27847932673498	0.0
33	8.324246031415704E-5	0.0	0.0	16.607620014817158	0.0
34	8.324246031415704E-5	0.0	0.0	16.94225470528007	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	20	0.001255791	40.000004	21
GTCGACT	20	0.001255791	40.000004	39
CTACGCG	60	0.0	40.000004	1
TTGCGGT	20	0.001255791	40.000004	12
ATACGAG	30	4.8676975E-6	40.000004	1
TGTAACG	20	0.001255791	40.000004	1
GTACGAG	30	4.8676975E-6	40.000004	1
AACGCGT	25	7.794865E-5	40.0	40
TAATGCG	25	7.794865E-5	40.0	1
CTTACCG	25	7.794865E-5	40.0	1
TACGGGA	345	0.0	37.101448	4
CACGACC	470	0.0	36.595745	27
CGTATGG	110	0.0	36.363636	2
ACTACGG	155	0.0	36.129032	2
CGTCATA	50	3.425157E-9	36.0	38
CTAAACG	45	5.44278E-8	35.555553	1
AAACACG	485	0.0	35.463917	40
GCCGATA	85	0.0	35.29412	9
ACGACCA	490	0.0	35.10204	28
CGAACCC	80	0.0	35.000004	34
>>END_MODULE