FastQCFastQC Report
Sat 18 Jun 2016
SRR3553751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511449
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21570.42174293038015526No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.3703204034028808No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG14850.29035153065114994No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCG8940.17479748713948018No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTA8270.16169745174983235No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7680.1501615996902917No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG200.001255115440.01
CTATGCG200.001255115440.01
CTCGTAC257.788579E-540.029
CTATCGG200.001255115440.02
CGTGAAC257.788579E-540.034
TATGCGG700.040.02
GCGCATG257.788579E-540.01
GTATGCG257.788579E-540.01
CTACGCG200.001255115440.01
ACTACGG257.788579E-540.02
GTATACG353.0519368E-740.01
CTCGAAC200.001255115440.013
CCGAAAG304.862208E-640.01
GCGTATG257.788579E-540.01
GTAACGG257.788579E-540.02
GTACGAG200.001255115440.01
GTTGCGC200.001255115440.09
AGGTCGA200.001255115440.06
CGGACGG353.0519368E-740.02
TCAAGCG1850.037.83783717
Produced by FastQC (version 0.11.5)