##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553751_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	511449
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98514807928063	33.0	31.0	34.0	30.0	34.0
2	32.130063799127576	33.0	31.0	34.0	30.0	34.0
3	32.0904723638134	33.0	31.0	34.0	30.0	34.0
4	35.81382112390483	37.0	35.0	37.0	35.0	37.0
5	35.63304454598601	37.0	35.0	37.0	33.0	37.0
6	35.62666463322834	37.0	35.0	37.0	33.0	37.0
7	35.858609558333285	37.0	35.0	37.0	35.0	37.0
8	35.654812112253616	37.0	35.0	37.0	35.0	37.0
9	37.47289759096215	39.0	37.0	39.0	35.0	39.0
10	36.96749235994205	39.0	37.0	39.0	33.0	39.0
11	36.887001441003896	39.0	37.0	39.0	33.0	39.0
12	36.819952722558845	39.0	35.0	39.0	33.0	39.0
13	35.09057794618818	39.0	35.0	39.0	30.0	39.0
14	36.87665045781691	40.0	36.0	41.0	30.0	41.0
15	37.545141353292316	40.0	36.0	41.0	32.0	41.0
16	37.778181206728334	40.0	36.0	41.0	33.0	41.0
17	37.80264698924037	40.0	36.0	41.0	33.0	41.0
18	37.83589761638013	39.0	37.0	41.0	33.0	41.0
19	37.834002999321534	40.0	36.0	41.0	33.0	41.0
20	37.75262440634354	40.0	36.0	41.0	33.0	41.0
21	37.663000611986725	39.0	36.0	41.0	33.0	41.0
22	37.64162018109332	39.0	36.0	41.0	33.0	41.0
23	37.56153790505016	39.0	36.0	41.0	33.0	41.0
24	37.50565941081124	39.0	35.0	41.0	33.0	41.0
25	37.322511139918156	39.0	35.0	41.0	32.0	41.0
26	37.29453181060086	39.0	35.0	41.0	32.0	41.0
27	37.263416293706705	39.0	35.0	41.0	32.0	41.0
28	37.15901683256786	39.0	35.0	41.0	32.0	41.0
29	37.116230552801944	39.0	35.0	41.0	32.0	41.0
30	36.99382343107524	39.0	35.0	41.0	31.0	41.0
31	36.924430392864195	39.0	35.0	41.0	31.0	41.0
32	36.786807677793874	39.0	35.0	41.0	31.0	41.0
33	36.67218823382194	39.0	35.0	41.0	31.0	41.0
34	36.50508261820827	39.0	35.0	41.0	30.0	41.0
35	36.40892640321909	39.0	35.0	40.0	30.0	41.0
36	36.257746129135064	39.0	35.0	40.0	30.0	41.0
37	36.22430584476653	39.0	35.0	40.0	30.0	41.0
38	36.117915960340135	38.0	35.0	40.0	30.0	41.0
39	35.99298463776447	38.0	35.0	40.0	30.0	41.0
40	35.88026763176778	38.0	35.0	40.0	29.0	41.0
41	35.77594442456628	38.0	35.0	40.0	29.0	41.0
42	35.71466363215101	38.0	35.0	40.0	29.0	41.0
43	35.65013715932576	38.0	35.0	40.0	29.0	41.0
44	35.567626488662604	38.0	34.0	40.0	29.0	41.0
45	35.50088083073776	38.0	34.0	40.0	28.0	41.0
46	34.550590576968574	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	16.0
10	25.0
11	24.0
12	10.0
13	22.0
14	11.0
15	28.0
16	34.0
17	75.0
18	116.0
19	215.0
20	434.0
21	695.0
22	1133.0
23	1608.0
24	2280.0
25	3139.0
26	3970.0
27	5127.0
28	6520.0
29	7707.0
30	9765.0
31	12324.0
32	16397.0
33	22685.0
34	36084.0
35	44256.0
36	49709.0
37	70571.0
38	105372.0
39	111088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.30928792509126	20.093303535640892	20.675766303189565	23.92164223607828
2	24.93425541940643	28.15569098776222	23.73433128229794	23.175722310533406
3	27.840508046745622	28.65994458880553	22.68437322196348	20.815174142485372
4	25.3415296539831	28.228425512612205	22.621414842926665	23.808629990478032
5	23.10396540026474	31.839147207248423	20.29488766230846	24.761999730178374
6	20.20123218541829	40.7923370658658	20.384632680873363	18.62179806784254
7	83.6628872086953	5.758345406873413	7.207756785134002	3.371010599297291
8	83.68537234406558	5.95308623147176	6.498595167846648	3.8629462566160067
9	77.82339979157257	7.094744539533757	8.855037354653152	6.2268183142405205
10	38.99548146540515	25.74587104481581	16.58816421578691	18.67048327399213
11	31.140739350355556	27.645962745063535	21.68564216569003	19.527655738890875
12	25.67118129080319	24.817528238397184	28.575674211896008	20.935616258903625
13	23.247088174969548	27.660235917950764	29.52220064952713	19.570475257552562
14	22.278076601968134	27.186679414760807	28.892616859158977	21.64262712411208
15	21.346018860140504	25.90346251532411	31.307129352095714	21.443389272439674
16	24.21082062923185	25.01363772340937	28.811279325993404	21.964262321365375
17	24.267522274948234	25.277202614532435	27.029870035917558	23.42540507460177
18	24.849593996664378	25.15910677310934	27.176121177282585	22.815178052943697
19	26.0514733629355	26.89065771953802	24.94559574854971	22.11227316897677
20	27.802772123906784	25.68897387618316	25.110421566959758	21.397832432950302
21	27.372035139378507	26.67636460331333	25.351501322712526	20.60009893459563
22	25.68838730743437	24.31131940819124	26.527180618204355	23.47311266617004
23	25.020872071311118	26.667370549165213	26.26224706666745	22.049510312856217
24	23.81078074255693	26.176608029344077	27.373403799792356	22.639207428306634
25	24.879900048685204	27.011686404705067	25.54761080772472	22.56080273888501
26	22.937575398524583	26.765718576045707	26.18618865224099	24.110517373188724
27	23.796116523837178	26.089013762858077	26.705106472004054	23.409763241300695
28	22.30251696650106	27.256090050034317	27.71283158242562	22.72856140103901
29	22.817328805022594	27.10612397326029	26.16585426894959	23.91069295276753
30	24.123226362745847	26.59111661182249	26.68320790538255	22.602449120049116
31	24.894759790321224	26.62064057217826	24.848811904999327	23.635787732501186
32	24.987242129713813	26.54712395566322	25.585151207647293	22.880482706975673
33	24.638429247099904	26.819878423850668	24.147275681446246	24.394416647603183
34	22.942463471431168	27.09771648786096	26.623573415922213	23.33624662478566
35	23.876476442421435	26.72524533237918	26.21297529176907	23.185302933430314
36	24.156074212678096	28.68555809083604	25.366947633097336	21.791420063388532
37	23.8504718945584	28.932112488244183	24.5091885994498	22.708227017747614
38	23.733158144800363	27.932990386138208	25.66883501580803	22.665016453253404
39	23.66061914286664	27.067410435840134	25.059976654563798	24.211993766729428
40	24.75750270310432	26.163312471038168	27.41074867679866	21.668436149058852
41	22.07082231072893	26.477126751640927	26.828872477998782	24.62317845963136
42	22.855846819526484	26.62533312216858	27.385330697684424	23.133489360620512
43	23.010896492123358	25.660427530408704	27.088331387880316	24.24034458958762
44	23.33702871645071	26.30330687908276	26.762199163552964	23.59746524091356
45	23.25979716452667	25.897596827836207	26.11208546697716	24.730520540659967
46	23.815473292547253	26.272414258313148	26.367047349784634	23.54506509935497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2433.0
1	2429.0
2	2425.0
3	3119.5
4	3814.0
5	2674.5
6	1535.0
7	1535.0
8	1502.5
9	1470.0
10	1438.5
11	1407.0
12	1404.0
13	1401.0
14	1430.0
15	1459.0
16	1433.5
17	1408.0
18	1492.5
19	1577.0
20	1577.0
21	1638.0
22	1699.0
23	1996.5
24	2294.0
25	2696.0
26	3098.0
27	4027.0
28	4956.0
29	5753.0
30	6550.0
31	6550.0
32	7812.0
33	9074.0
34	10429.5
35	11785.0
36	13140.5
37	14496.0
38	16102.0
39	17708.0
40	20695.5
41	23683.0
42	26671.5
43	29660.0
44	29660.0
45	34438.0
46	39216.0
47	39984.0
48	40752.0
49	39269.0
50	37786.0
51	37988.0
52	38190.0
53	35925.5
54	33661.0
55	32740.5
56	31820.0
57	31820.0
58	30787.0
59	29754.0
60	28619.0
61	27484.0
62	25101.5
63	22719.0
64	20956.0
65	19193.0
66	17279.5
67	15366.0
68	13361.0
69	11356.0
70	11356.0
71	9478.5
72	7601.0
73	6409.5
74	5218.0
75	4211.5
76	3205.0
77	2570.5
78	1936.0
79	1593.5
80	1251.0
81	1251.0
82	934.0
83	617.0
84	407.0
85	197.0
86	158.0
87	119.0
88	82.0
89	45.0
90	33.5
91	22.0
92	13.0
93	4.0
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	511449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.193785419402175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.95098460031726	21.422788043172876
2	8.654533728284246	5.226262685935864
3	3.7295640798373895	3.378289726035608
4	2.2499905108746763	2.717429227241642
5	1.6168568544457922	2.440951445851291
6	1.2737445525430653	2.307550181917066
7	1.0235315496902102	2.1633004386934065
8	0.8956177684282434	2.1633672574180967
9	0.8012415042682652	2.1773262644095515
>10	8.672033349568748	50.97242778370678
>50	0.10317048156067667	1.953251191903903
>100	0.024160176061677446	1.3950949262049075
>500	0.0026119109255867507	0.5904902916879167
>1k	0.0019589331941900633	1.0914705358211374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2157	0.42174293038015526	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1894	0.3703204034028808	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	1485	0.29035153065114994	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCG	894	0.17479748713948018	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTA	827	0.16169745174983235	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	768	0.1501615996902917	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9552291626340066E-4	0.0	0.0	0.04946729781464036	0.0
2	1.9552291626340066E-4	0.0	0.0	0.29113362231620354	0.0
3	1.9552291626340066E-4	0.0	0.0	0.3947607679358059	0.0
4	1.9552291626340066E-4	0.0	0.0	0.5908702529479968	0.0
5	1.9552291626340066E-4	0.0	0.0	1.194645018369378	0.0
6	1.9552291626340066E-4	0.0	0.0	1.8512109711818774	0.0
7	1.9552291626340066E-4	0.0	0.0	2.266697168241604	0.0
8	1.9552291626340066E-4	0.0	0.0	3.054458997866845	0.0
9	1.9552291626340066E-4	0.0	0.0	3.421064465860721	0.0
10	1.9552291626340066E-4	0.0	0.0	4.137069385217295	0.0
11	1.9552291626340066E-4	0.0	0.0	4.67671263410428	0.0
12	1.9552291626340066E-4	0.0	0.0	5.324871101517453	0.0
13	1.9552291626340066E-4	0.0	0.0	5.551677684382998	0.0
14	1.9552291626340066E-4	0.0	0.0	5.674466075796413	0.0
15	1.9552291626340066E-4	0.0	0.0	5.818370942166276	0.0
16	1.9552291626340066E-4	0.0	0.0	6.091907502018774	0.0
17	1.9552291626340066E-4	0.0	0.0	6.382259032669924	0.0
18	1.9552291626340066E-4	0.0	0.0	6.738892831934367	0.0
19	1.9552291626340066E-4	0.0	0.0	6.934611271114031	0.0
20	1.9552291626340066E-4	0.0	0.0	7.133067031121382	0.0
21	1.9552291626340066E-4	0.0	0.0	7.335824295286529	0.0
22	1.9552291626340066E-4	0.0	0.0	7.567323428142395	0.0
23	1.9552291626340066E-4	0.0	0.0	7.793152396426623	0.0
24	1.9552291626340066E-4	0.0	0.0	7.992390248099029	0.0
25	1.9552291626340066E-4	0.0	0.0	8.156825020676548	0.0
26	1.9552291626340066E-4	0.0	0.0	8.316371720347483	0.0
27	1.9552291626340066E-4	0.0	0.0	8.496252803309812	0.0
28	1.9552291626340066E-4	0.0	0.0	8.67672045502093	0.0
29	1.9552291626340066E-4	0.0	0.0	8.874785169195755	0.0
30	1.9552291626340066E-4	0.0	0.0	9.14128290406277	0.0
31	1.9552291626340066E-4	0.0	0.0	9.338370003656278	0.0
32	1.9552291626340066E-4	0.0	0.0	9.542104882402741	0.0
33	1.9552291626340066E-4	0.0	0.0	9.744080054902835	0.0
34	1.9552291626340066E-4	0.0	0.0	9.939798494082499	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	0.0012551154	40.0	1
CTATGCG	20	0.0012551154	40.0	1
CTCGTAC	25	7.788579E-5	40.0	29
CTATCGG	20	0.0012551154	40.0	2
CGTGAAC	25	7.788579E-5	40.0	34
TATGCGG	70	0.0	40.0	2
GCGCATG	25	7.788579E-5	40.0	1
GTATGCG	25	7.788579E-5	40.0	1
CTACGCG	20	0.0012551154	40.0	1
ACTACGG	25	7.788579E-5	40.0	2
GTATACG	35	3.0519368E-7	40.0	1
CTCGAAC	20	0.0012551154	40.0	13
CCGAAAG	30	4.862208E-6	40.0	1
GCGTATG	25	7.788579E-5	40.0	1
GTAACGG	25	7.788579E-5	40.0	2
GTACGAG	20	0.0012551154	40.0	1
GTTGCGC	20	0.0012551154	40.0	9
AGGTCGA	20	0.0012551154	40.0	6
CGGACGG	35	3.0519368E-7	40.0	2
TCAAGCG	185	0.0	37.837837	17
>>END_MODULE