FastQCFastQC Report
Sat 18 Jun 2016
SRR3553750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570573
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48300.8465174482493913No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.3231838870749229No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16850.2953171636232349No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT10770.18875761734256616No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10640.1864792059911703No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT9840.17245821305950335No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT9570.16772612794506575No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGT7010.12285895056373154No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA6760.11847739027258564No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA5770.1011264115196478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA200.001255237140.00000424
GCGCGTT200.001255237140.00000410
TAAACCG200.001255237140.0000041
ATCGTTG200.001255237140.00000423
CGAATAT200.001255237140.00000414
ACGTCGT200.001255237140.00000431
CGTCGTT200.001255237140.00000432
TCATACG200.001255237140.0000041
TTACCGG200.001255237140.0000042
TAATCGT200.001255237140.00000421
CTATACG304.863199E-640.01
TCGTGGA257.7897144E-540.035
CGCTAGA257.7897144E-540.024
GTGTAAC257.7897144E-540.013
GCGTATG304.863199E-640.01
ATCGCCC304.863199E-640.016
AATCGCC304.863199E-640.015
GTAAGCG353.0527735E-739.9999961
ACGGGCG2650.036.9811335
CATACGG650.036.9230772