##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553750_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	570573
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739575829911335	31.0	31.0	34.0	30.0	34.0
2	31.879918958660856	33.0	31.0	34.0	30.0	34.0
3	31.86178455692786	33.0	31.0	34.0	30.0	34.0
4	35.62218857183919	37.0	35.0	37.0	33.0	37.0
5	35.40968990821508	37.0	35.0	37.0	33.0	37.0
6	35.42684985093932	37.0	35.0	37.0	33.0	37.0
7	35.71055938503925	37.0	35.0	37.0	35.0	37.0
8	35.50841171944694	37.0	35.0	37.0	33.0	37.0
9	37.30826029272328	39.0	37.0	39.0	34.0	39.0
10	36.760819386826924	39.0	37.0	39.0	32.0	39.0
11	36.69352563125139	39.0	35.0	39.0	32.0	39.0
12	36.61431052643571	39.0	35.0	39.0	32.0	39.0
13	34.83880239688874	38.0	35.0	39.0	29.0	39.0
14	36.55164895639997	39.0	36.0	41.0	29.0	41.0
15	37.23830254849073	39.0	36.0	41.0	32.0	41.0
16	37.50274373305432	39.0	36.0	41.0	32.0	41.0
17	37.557153948749765	39.0	36.0	41.0	32.0	41.0
18	37.573039733741346	39.0	36.0	41.0	33.0	41.0
19	37.52293922074826	39.0	36.0	41.0	32.0	41.0
20	37.46959810576386	39.0	35.0	41.0	32.0	41.0
21	37.341637266397115	39.0	35.0	41.0	32.0	41.0
22	37.339385144407466	39.0	35.0	41.0	32.0	41.0
23	37.266477733786914	39.0	35.0	41.0	32.0	41.0
24	37.144884177835266	39.0	35.0	41.0	32.0	41.0
25	36.95005547055329	39.0	35.0	41.0	31.0	41.0
26	36.91004656722277	39.0	35.0	41.0	31.0	41.0
27	36.883557406326624	39.0	35.0	40.0	31.0	41.0
28	36.75483768071746	39.0	35.0	40.0	31.0	41.0
29	36.710065145038406	39.0	35.0	40.0	31.0	41.0
30	36.58380435106463	39.0	35.0	40.0	31.0	41.0
31	36.50074223631332	39.0	35.0	40.0	30.0	41.0
32	36.40213960352137	39.0	35.0	40.0	30.0	41.0
33	36.315437288480176	38.0	35.0	40.0	30.0	41.0
34	36.17075641504242	38.0	35.0	40.0	30.0	41.0
35	36.10784597238215	38.0	35.0	40.0	30.0	41.0
36	35.97902459457423	38.0	35.0	40.0	29.0	41.0
37	35.905083135724965	38.0	35.0	40.0	29.0	41.0
38	35.84455275661484	38.0	35.0	40.0	29.0	41.0
39	35.740555546792436	38.0	35.0	40.0	29.0	41.0
40	35.61723565608607	38.0	35.0	40.0	28.0	41.0
41	35.49141827601376	38.0	34.0	40.0	28.0	41.0
42	35.46827487455593	38.0	34.0	40.0	28.0	41.0
43	35.399293341956245	38.0	34.0	40.0	28.0	41.0
44	35.28005180756888	38.0	34.0	40.0	27.0	41.0
45	35.18822131436293	37.0	34.0	40.0	27.0	41.0
46	34.20753172687807	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	13.0
11	17.0
12	8.0
13	11.0
14	16.0
15	26.0
16	38.0
17	82.0
18	164.0
19	347.0
20	604.0
21	1027.0
22	1583.0
23	2227.0
24	2971.0
25	3961.0
26	5255.0
27	6548.0
28	8178.0
29	10096.0
30	12565.0
31	15994.0
32	20522.0
33	27812.0
34	42121.0
35	53372.0
36	57088.0
37	77489.0
38	111339.0
39	109087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.22694904946431	22.318441286215787	20.75983265944936	23.69477700487054
2	24.241420466793905	28.452099906585133	23.47219374208033	23.83428588454063
3	27.736854004658472	27.447320500619554	24.11926256587676	20.696562928845214
4	25.002409858160128	28.28858007651957	22.67194557050544	24.03706449481486
5	21.685218192939377	33.17962819831993	20.528661538488503	24.606492070252184
6	20.133269537815494	41.07099354508538	20.97172491512918	17.82401200196995
7	80.63823559824948	7.828621403396236	7.198027246294513	4.335115752059771
8	79.95699060418211	6.846626110944612	8.033503162610218	5.162880122263058
9	74.72908812719845	8.795018341211378	10.353977492801096	6.121916038789077
10	37.032597055942006	27.056660585060982	17.148901192310188	18.76184116668682
11	29.915365781416227	27.57631363559089	22.946231244731173	19.562089338261714
12	25.736058313309602	25.30088174519299	28.489255537854053	20.473804403643356
13	23.16741240822822	28.571628871327597	28.604753467128656	19.656205253315527
14	21.205875497088016	29.6028729014517	28.16659743801407	21.02465416344622
15	20.40036945316375	27.802927933848952	31.690423486565262	20.106279126422034
16	21.87748105851486	24.831704269217088	31.15745049275027	22.133364179517784
17	22.031010931116615	25.379574568021972	27.332874145814824	25.256540355046592
18	22.851414280030777	26.469531506047428	28.043037437803754	22.636016776118044
19	24.655039758278082	27.443464727563345	26.07168583161138	21.829809682547193
20	25.93568220017421	26.673361690791538	25.347151021867493	22.04380508716676
21	26.11059408699676	27.466248841077302	26.059943250031107	20.36321382189483
22	23.480255813016036	25.717480497675144	25.73465621401644	25.06760747529238
23	22.987593173879592	27.865146089983227	25.68733536287206	23.459925373265122
24	22.840898535332027	26.71612571923312	27.968375650442624	22.47460009499223
25	23.348633741870014	27.79959794802768	25.090216326394692	23.76155198370761
26	21.32487867459554	28.407407991615447	26.623236641060828	23.644476692728187
27	22.237820576858702	27.879167082914897	26.263598172363572	23.619414167862832
28	21.03481938332168	28.237228189907338	28.010088104414333	22.717864322356647
29	22.4714453715826	27.988180302958604	26.61131879706891	22.92905552838988
30	24.10787050911978	26.950977350838546	26.369631931409305	22.571520208632375
31	24.635585630585393	28.349921920595612	24.06405490620832	22.950437542610675
32	24.517458765136098	28.28998217581273	25.02344134755763	22.169117711493534
33	24.18148072201103	27.00215397503913	25.43653485180687	23.379830451142976
34	23.022295131385466	27.68445054357637	26.11111987423169	23.18213445080647
35	23.43977019592585	26.357363562594095	26.51842971889662	23.68443652258344
36	24.206017459641448	27.7736591111041	26.292516470285133	21.727806958969317
37	22.300389257816267	29.312988872589486	26.94449264160765	21.4421292279866
38	22.259202591079493	28.971402432291747	26.62533979000058	22.14405518662818
39	23.125524691844866	25.867505122043983	27.471156188603384	23.535813997507766
40	23.960825345748923	25.738336724661	28.583897240142804	21.716940689447277
41	20.514815807968482	26.162822285667215	28.97438189328973	24.347980013074576
42	22.54978766958829	26.22416412974326	28.2642186013008	22.961829599367654
43	21.982638505502365	24.742670964101	29.45039460331982	23.824295927076815
44	23.079605939993655	25.856814114933584	27.297295876250715	23.766284068822046
45	23.178979727396847	25.3257690076467	27.707760444325263	23.78749082063119
46	23.66673501900721	25.854535703582187	27.18127215974117	23.29745711766943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5348.0
1	4966.5
2	4585.0
3	4401.0
4	4217.0
5	3019.0
6	1821.0
7	1821.0
8	1767.5
9	1714.0
10	1853.5
11	1993.0
12	2002.5
13	2012.0
14	1905.5
15	1799.0
16	1783.5
17	1768.0
18	1880.5
19	1993.0
20	1993.0
21	2207.0
22	2421.0
23	2589.5
24	2758.0
25	3345.5
26	3933.0
27	4255.5
28	4578.0
29	5759.5
30	6941.0
31	6941.0
32	8047.5
33	9154.0
34	10730.0
35	12306.0
36	14000.5
37	15695.0
38	18731.5
39	21768.0
40	24646.5
41	27525.0
42	32425.5
43	37326.0
44	37326.0
45	42766.0
46	48206.0
47	49764.5
48	51323.0
49	48599.0
50	45875.0
51	43757.5
52	41640.0
53	38971.0
54	36302.0
55	34357.0
56	32412.0
57	32412.0
58	31393.0
59	30374.0
60	28688.0
61	27002.0
62	25094.5
63	23187.0
64	20756.5
65	18326.0
66	16178.5
67	14031.0
68	12009.5
69	9988.0
70	9988.0
71	8726.0
72	7464.0
73	6349.0
74	5234.0
75	4104.5
76	2975.0
77	2502.0
78	2029.0
79	1741.0
80	1453.0
81	1453.0
82	1036.0
83	619.0
84	417.0
85	215.0
86	214.5
87	214.0
88	119.0
89	24.0
90	19.0
91	14.0
92	11.5
93	9.0
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	570573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.799564481423403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91279892315849	22.323687819945672
2	9.256143708834436	5.516581026014667
3	3.6105227067078256	3.2277601263054962
4	1.846453073595581	2.2009398971413576
5	1.1678976447709402	1.7401420586527077
6	0.833585156727069	1.4904284777187435
7	0.6127642427590771	1.278207529480679
8	0.5495288693914241	1.3100576782266753
9	0.4372931901741855	1.1728031956094689
>10	6.09531423965949	42.69704946155596
>50	0.61781414740048	11.406263162255927
>100	0.053362066474129756	2.994106534498024
>500	0.003557471098275317	0.7867789683771805
>1k	0.0029645592485627644	1.8551940642174702
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4830	0.8465174482493913	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1844	0.3231838870749229	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1685	0.2953171636232349	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	1077	0.18875761734256616	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1064	0.1864792059911703	No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	984	0.17245821305950335	No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	957	0.16772612794506575	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGT	701	0.12285895056373154	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	676	0.11847739027258564	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA	577	0.1011264115196478	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5052482329167345E-4	0.0	0.0	0.19173707834054537	0.0
2	3.5052482329167345E-4	0.0	0.0	0.7368031785590976	0.0
3	3.5052482329167345E-4	0.0	0.0	0.9621906399356437	0.0
4	3.5052482329167345E-4	0.0	0.0	1.2759103567816914	0.0
5	3.5052482329167345E-4	0.0	0.0	2.088952684406728	0.0
6	3.5052482329167345E-4	0.0	0.0	3.0294107852982877	0.0
7	3.5052482329167345E-4	0.0	0.0	3.6698196374521754	0.0
8	3.5052482329167345E-4	0.0	0.0	4.867037171404886	0.0
9	3.5052482329167345E-4	0.0	0.0	5.43138213690448	0.0
10	3.5052482329167345E-4	0.0	0.0	6.448254649273625	0.0
11	3.5052482329167345E-4	0.0	0.0	7.615502310834898	0.0
12	3.5052482329167345E-4	0.0	0.0	8.528093688274769	0.0
13	3.5052482329167345E-4	0.0	0.0	8.92295990171284	0.0
14	3.5052482329167345E-4	0.0	0.0	9.186379306416532	0.0
15	3.5052482329167345E-4	0.0	0.0	9.442087165007807	0.0
16	3.5052482329167345E-4	0.0	0.0	10.093362286683737	0.0
17	3.5052482329167345E-4	0.0	0.0	10.71203859979354	0.0
18	3.5052482329167345E-4	0.0	0.0	11.527184076358328	0.0
19	3.5052482329167345E-4	0.0	0.0	11.891905154993314	0.0
20	5.257872349375102E-4	0.0	0.0	12.263286205270841	0.0
21	5.257872349375102E-4	0.0	0.0	12.683214943574267	0.0
22	5.257872349375102E-4	0.0	0.0	13.094380561295399	0.0
23	7.010496465833469E-4	0.0	0.0	13.576877980556388	0.0
24	7.010496465833469E-4	0.0	0.0	13.914783910209561	0.0
25	8.763120582291836E-4	0.0	0.0	14.217812619945214	0.0
26	8.763120582291836E-4	0.0	0.0	14.509449272923884	0.0
27	8.763120582291836E-4	0.0	0.0	14.82246794012335	0.0
28	8.763120582291836E-4	0.0	0.0	15.133909245618002	0.0
29	8.763120582291836E-4	0.0	0.0	15.448154749698986	0.0
30	8.763120582291836E-4	0.0	0.0	15.806566381514722	0.0
31	8.763120582291836E-4	0.0	0.0	16.121337672830645	0.0
32	8.763120582291836E-4	0.0	0.0	16.43645948896986	0.0
33	8.763120582291836E-4	0.0	0.0	16.759117588809847	0.0
34	8.763120582291836E-4	0.0	0.0	17.078620965240205	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	20	0.0012552371	40.000004	24
GCGCGTT	20	0.0012552371	40.000004	10
TAAACCG	20	0.0012552371	40.000004	1
ATCGTTG	20	0.0012552371	40.000004	23
CGAATAT	20	0.0012552371	40.000004	14
ACGTCGT	20	0.0012552371	40.000004	31
CGTCGTT	20	0.0012552371	40.000004	32
TCATACG	20	0.0012552371	40.000004	1
TTACCGG	20	0.0012552371	40.000004	2
TAATCGT	20	0.0012552371	40.000004	21
CTATACG	30	4.863199E-6	40.0	1
TCGTGGA	25	7.7897144E-5	40.0	35
CGCTAGA	25	7.7897144E-5	40.0	24
GTGTAAC	25	7.7897144E-5	40.0	13
GCGTATG	30	4.863199E-6	40.0	1
ATCGCCC	30	4.863199E-6	40.0	16
AATCGCC	30	4.863199E-6	40.0	15
GTAAGCG	35	3.0527735E-7	39.999996	1
ACGGGCG	265	0.0	36.981133	5
CATACGG	65	0.0	36.923077	2
>>END_MODULE