FastQCFastQC Report
Sat 18 Jun 2016
SRR3553749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655936
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.5508159332617817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17430.2657271441116207No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC200.001255374540.0000049
TAACACG200.001255374540.0000041
CATCGTT200.001255374540.00000436
TTCGAAT200.001255374540.00000416
TCGAACG200.001255374540.0000041
TCGATCA850.040.017
ACGATAG304.864316E-640.01
CAATGCG451.2205419E-940.01
CCGTTAG257.790989E-540.01
CTCGATC900.037.7777816
CGTTTTT15350.036.351791
GGCCGAT1500.036.08
GCCGATC503.4197E-936.09
CGTAAGG950.035.789472
GCCGATA408.69235E-735.0000049
TCTACGG408.69235E-735.0000042
CAAGCCG408.69235E-735.0000041
CACAACG1850.034.59459312
CTATGCG351.4001533E-534.2857131
GTTAGCG351.4001533E-534.2857131
Produced by FastQC (version 0.11.5)