FastQCFastQC Report
Sat 18 Jun 2016
SRR3553748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677179
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35050.5175884072010503No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.3046461866064955No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11460.1692314735099582No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7620.11252563945426543No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG257.791258E-540.01
TTAGGCG200.001255403440.01
TGCCACG257.791258E-540.01
TAGCGTG257.791258E-540.01
GACGTCC257.791258E-540.019
TATTGCG304.864549E-640.01
TTATACG200.001255403440.01
ACGATAG353.0539013E-740.01
ACCTACG200.001255403440.026
CTACGCA451.2205419E-940.015
TACGAAG257.791258E-540.01
CGTACTA200.001255403440.015
TATAGCG401.9263098E-840.01
TTCGGAA200.001255403440.014
GTACGAG200.001255403440.01
CTAACGG1000.036.02
CGGGTAT1450.035.8620726
GCGTTAG455.4367774E-835.5555571
AGTACGG900.035.5555572
AACGGGA3550.035.4929544
Produced by FastQC (version 0.11.5)