##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553748_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677179
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879731946796934	31.0	31.0	34.0	30.0	34.0
2	32.01292125124967	33.0	31.0	34.0	30.0	34.0
3	31.993279472635745	33.0	31.0	34.0	30.0	34.0
4	35.716847391900814	37.0	35.0	37.0	33.0	37.0
5	35.531664449133835	37.0	35.0	37.0	33.0	37.0
6	35.52221052336236	37.0	35.0	37.0	33.0	37.0
7	35.81329751808606	37.0	35.0	37.0	35.0	37.0
8	35.62842911549236	37.0	35.0	37.0	35.0	37.0
9	37.40775481815	39.0	37.0	39.0	35.0	39.0
10	36.850918294867384	39.0	37.0	39.0	32.0	39.0
11	36.794005720791695	39.0	37.0	39.0	32.0	39.0
12	36.718628309501625	39.0	35.0	39.0	32.0	39.0
13	35.028775257354404	39.0	35.0	39.0	30.0	39.0
14	36.76886022750263	39.0	36.0	41.0	30.0	41.0
15	37.44537559493133	40.0	36.0	41.0	32.0	41.0
16	37.64657202896132	40.0	36.0	41.0	33.0	41.0
17	37.718699191794194	39.0	36.0	41.0	33.0	41.0
18	37.753354726003025	39.0	36.0	41.0	33.0	41.0
19	37.7252720477156	39.0	36.0	41.0	33.0	41.0
20	37.677667204682955	39.0	36.0	41.0	33.0	41.0
21	37.567102641989784	39.0	36.0	41.0	33.0	41.0
22	37.56160778760121	39.0	36.0	41.0	33.0	41.0
23	37.481869638603676	39.0	35.0	41.0	32.0	41.0
24	37.39333913189866	39.0	35.0	41.0	32.0	41.0
25	37.19685341689568	39.0	35.0	41.0	32.0	41.0
26	37.1519066598344	39.0	35.0	41.0	32.0	41.0
27	37.120480700080776	39.0	35.0	41.0	32.0	41.0
28	37.011324922952426	39.0	35.0	41.0	31.0	41.0
29	36.96035317102273	39.0	35.0	41.0	31.0	41.0
30	36.80571606620997	39.0	35.0	41.0	31.0	41.0
31	36.75615605327395	39.0	35.0	41.0	31.0	41.0
32	36.649327578084964	39.0	35.0	40.0	31.0	41.0
33	36.530543622882576	39.0	35.0	40.0	30.0	41.0
34	36.40356685603068	39.0	35.0	40.0	30.0	41.0
35	36.34147839788298	39.0	35.0	40.0	30.0	41.0
36	36.20237780557282	39.0	35.0	40.0	30.0	41.0
37	36.17307831459629	38.0	35.0	40.0	30.0	41.0
38	36.075628452742926	38.0	35.0	40.0	30.0	41.0
39	35.953950137260605	38.0	35.0	40.0	29.0	41.0
40	35.86298157503408	38.0	35.0	40.0	29.0	41.0
41	35.76398116303075	38.0	35.0	40.0	29.0	41.0
42	35.69262189170072	38.0	35.0	40.0	29.0	41.0
43	35.61361323963088	38.0	35.0	40.0	28.0	41.0
44	35.506232473245625	38.0	34.0	40.0	28.0	41.0
45	35.41500696270853	38.0	34.0	40.0	28.0	41.0
46	34.44837923207896	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	6.0
9	27.0
10	32.0
11	36.0
12	19.0
13	22.0
14	22.0
15	38.0
16	47.0
17	123.0
18	201.0
19	371.0
20	663.0
21	1051.0
22	1565.0
23	2296.0
24	3160.0
25	4217.0
26	5667.0
27	7373.0
28	9234.0
29	11082.0
30	13704.0
31	17394.0
32	22569.0
33	31024.0
34	46751.0
35	59807.0
36	65368.0
37	91940.0
38	136514.0
39	144852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.31751427613674	20.670457884842854	20.13116177554236	24.880866063478045
2	24.164807237082073	27.849062064830715	24.250899688265584	23.73523100982163
3	28.96383378693078	26.636531847561724	23.423348922515316	20.97628544299218
4	24.818844057479634	29.22388319779556	21.959924923838454	23.997347820886354
5	22.216430220074752	32.98433649005654	20.243982757882332	24.555250531986374
6	20.575357475645287	41.00511090863716	20.492809139090255	17.9267224766273
7	83.8931803850976	6.256543690811439	6.001219766117969	3.8490561579730027
8	84.03479729879398	5.641639802770021	6.1858090696846775	4.137753828751334
9	77.76112372061154	8.415352513884807	8.560661213652521	5.262862551851136
10	37.532026244168826	26.972927394381692	16.441147761522434	19.05389859992705
11	30.968473623665233	28.498964084828383	21.19011369224385	19.34244859926253
12	25.215046538655216	25.913827806237343	27.692382663963294	21.178742991144144
13	23.505749587627495	28.15577565163716	28.606764238111342	19.731710522624006
14	22.526244907181116	28.593473808254537	27.833261220445404	21.04702006411894
15	21.46980340500813	27.12030349434935	30.448965487707092	20.96092761293543
16	22.98314035136943	25.76157854865552	29.928423651648973	21.32685744832607
17	23.037335770896618	26.148773071817054	26.65956859264685	24.154322564639482
18	23.738184438678694	25.356663452351597	27.78556334440377	23.119588764565943
19	25.397568442021978	27.314786784587238	25.663820053486596	21.623824719904192
20	27.48948210148277	26.106096024832432	25.01362269060322	21.390799183081576
21	27.154267926205627	26.96834957965324	25.641373994172884	20.23600849996825
22	25.44821974692068	25.74666373292733	24.510506084801804	24.29461043535018
23	24.225500200094803	27.648376573992987	25.57049170160327	22.555631524308932
24	23.70377699249386	27.06802780357926	27.334427086486734	21.893768117440146
25	24.48097179623113	27.56464686589513	25.063240295401958	22.891141042471784
26	22.15175012810498	28.781902569335433	25.181968135456064	23.88437916710353
27	23.33489372824615	27.83872506383098	25.885474889209497	22.94090631871337
28	22.364397005813824	28.370342258103104	26.92257143236869	22.34268930371438
29	23.507816987827443	27.719406538005458	25.784910636626357	22.98786583754074
30	23.75974446933529	27.236520919874952	26.204740548658478	22.798994062131285
31	25.425478344721263	27.510599117810802	24.221070056809204	22.84285248065873
32	26.023104673948836	27.288648939202194	23.88836629606057	22.799880090788402
33	24.603686765242276	27.888047325744004	23.78086148566332	23.7274044233504
34	23.45509828272879	27.5501750644955	25.642850708601415	23.35187594417429
35	24.2954964640073	26.603158101476865	25.606818876545194	23.49452655797064
36	24.65611012745522	28.687097503023573	23.988782877200858	22.668009492320344
37	23.680297233080175	28.709248219451577	24.80821171359419	22.802242833874057
38	24.208960998495225	28.24482153167774	24.85428520376444	22.691932266062594
39	23.05682840135326	27.42317762364161	24.791377169108905	24.728616805896227
40	24.05597338369914	25.961673353721835	27.041446943865655	22.94090631871337
41	21.560473670920096	26.611575373719504	26.997145511009645	24.830805444350755
42	22.927615888856565	26.583222456691658	26.43953814279533	24.049623511656446
43	22.96807786419839	26.116285354389312	26.372642979182757	24.542993802229542
44	23.53129674724113	26.63416910447607	26.248155952857367	23.586378195425432
45	23.765798998492276	26.37766380823977	25.888871332395127	23.967665860872827
46	22.634488074792632	27.214665546332657	25.87321815945267	24.27762821942204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3830.0
1	3692.0
2	3554.0
3	4039.0
4	4524.0
5	3323.0
6	2122.0
7	2122.0
8	2002.0
9	1882.0
10	1991.0
11	2100.0
12	2062.0
13	2024.0
14	1969.5
15	1915.0
16	1921.0
17	1927.0
18	1946.0
19	1965.0
20	1965.0
21	2206.5
22	2448.0
23	2743.5
24	3039.0
25	4132.5
26	5226.0
27	5720.5
28	6215.0
29	7926.0
30	9637.0
31	9637.0
32	11123.5
33	12610.0
34	13862.5
35	15115.0
36	17265.5
37	19416.0
38	21884.5
39	24353.0
40	26355.0
41	28357.0
42	32263.5
43	36170.0
44	36170.0
45	40570.0
46	44970.0
47	48057.5
48	51145.0
49	54462.5
50	57780.0
51	57347.5
52	56915.0
53	53756.5
54	50598.0
55	46743.5
56	42889.0
57	42889.0
58	41445.5
59	40002.0
60	36915.5
61	33829.0
62	31234.5
63	28640.0
64	25971.0
65	23302.0
66	20912.5
67	18523.0
68	15811.0
69	13099.0
70	13099.0
71	11601.0
72	10103.0
73	8449.0
74	6795.0
75	5412.5
76	4030.0
77	3439.5
78	2849.0
79	2250.5
80	1652.0
81	1652.0
82	1244.5
83	837.0
84	652.5
85	468.0
86	335.0
87	202.0
88	144.5
89	87.0
90	50.0
91	13.0
92	16.0
93	19.0
94	19.0
95	10.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	677179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.996503027449943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35227733414402	20.536072544557108
2	8.812826631746361	4.934566549521569
3	3.4869829460675255	2.928699858187374
4	1.887054728069911	2.1132373362949197
5	1.209835896690922	1.6935587172212507
6	0.9249161381026805	1.5536650476317397
7	0.782116563016722	1.532757010702148
8	0.6851379953005681	1.534517436772267
9	0.5613790414171734	1.414498502932456
>10	7.778751753338456	50.548849359479085
>50	0.47571544627090895	8.072626097245568
>100	0.0398199313284706	1.8535721046719986
>500	0.001592797253138824	0.28539361996553625
>1k	0.001592797253138824	0.9979858148169846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3505	0.5175884072010503	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2063	0.3046461866064955	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1146	0.1692314735099582	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	762	0.11252563945426543	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05862556281278657	0.0
2	0.0	0.0	0.0	0.2737828550501418	0.0
3	0.0	0.0	0.0	0.37700519360464513	0.0
4	0.0	0.0	0.0	0.4994248197300861	0.0
5	0.0	0.0	0.0	0.8712615128348634	0.0
6	0.0	0.0	0.0	1.502113916704446	0.0
7	0.0	0.0	0.0	1.8879793968802931	0.0
8	0.0	0.0	0.0	2.6629591289747614	0.0
9	0.0	0.0	0.0	3.028002935708284	0.0
10	1.4767144285336668E-4	0.0	0.0	3.6520624532066117	0.0
11	1.4767144285336668E-4	0.0	0.0	4.255447968705468	0.0
12	1.4767144285336668E-4	0.0	0.0	4.77968159083492	0.0
13	2.9534288570673336E-4	0.0	0.0	5.027474271942869	0.0
14	2.9534288570673336E-4	0.0	0.0	5.15166595538255	0.0
15	2.9534288570673336E-4	0.0	0.0	5.287966697136207	0.0
16	2.9534288570673336E-4	0.0	0.0	5.587739726128542	0.0
17	2.9534288570673336E-4	0.0	0.0	5.921624858420004	0.0
18	2.9534288570673336E-4	0.0	0.0	6.351348757123302	0.0
19	2.9534288570673336E-4	0.0	0.0	6.543616975718384	0.0
20	2.9534288570673336E-4	0.0	0.0	6.741496709141896	0.0
21	2.9534288570673336E-4	0.0	0.0	6.9830871896500035	0.0
22	2.9534288570673336E-4	0.0	0.0	7.2189184838868306	0.0
23	2.9534288570673336E-4	0.0	0.0	7.486646809779985	0.0
24	2.9534288570673336E-4	0.0	0.0	7.6964879300746185	0.0
25	2.9534288570673336E-4	0.0	0.0	7.898797806783731	0.0
26	2.9534288570673336E-4	0.0	0.0	8.078218609850571	0.0
27	2.9534288570673336E-4	0.0	0.0	8.286878358602378	0.0
28	2.9534288570673336E-4	0.0	0.0	8.476931505554662	0.0
29	2.9534288570673336E-4	0.0	0.0	8.699620041377539	0.0
30	2.9534288570673336E-4	0.0	0.0	8.961884523885118	0.0
31	2.9534288570673336E-4	0.0	0.0	9.174088387265405	0.0
32	2.9534288570673336E-4	0.0	0.0	9.406227895430899	0.0
33	2.9534288570673336E-4	0.0	0.0	9.623895602196761	0.0
34	2.9534288570673336E-4	0.0	0.0	9.851604967076652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	7.791258E-5	40.0	1
TTAGGCG	20	0.0012554034	40.0	1
TGCCACG	25	7.791258E-5	40.0	1
TAGCGTG	25	7.791258E-5	40.0	1
GACGTCC	25	7.791258E-5	40.0	19
TATTGCG	30	4.864549E-6	40.0	1
TTATACG	20	0.0012554034	40.0	1
ACGATAG	35	3.0539013E-7	40.0	1
ACCTACG	20	0.0012554034	40.0	26
CTACGCA	45	1.2205419E-9	40.0	15
TACGAAG	25	7.791258E-5	40.0	1
CGTACTA	20	0.0012554034	40.0	15
TATAGCG	40	1.9263098E-8	40.0	1
TTCGGAA	20	0.0012554034	40.0	14
GTACGAG	20	0.0012554034	40.0	1
CTAACGG	100	0.0	36.0	2
CGGGTAT	145	0.0	35.862072	6
GCGTTAG	45	5.4367774E-8	35.555557	1
AGTACGG	90	0.0	35.555557	2
AACGGGA	355	0.0	35.492954	4
>>END_MODULE