FastQCFastQC Report
Sat 18 Jun 2016
SRR3553747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060441
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69420.6546333082179961No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30260.28535298050528035No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG19360.18256555527370216No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTA10880.10259882445133675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGG401.9281288E-840.0000042
ACGATTG257.794245E-540.01
GTCAACG257.794245E-540.01
TAATGCG750.040.01
ATTACCG353.056084E-739.9999961
CGACAGG2550.037.647062
CTATACG650.036.9230771
CGTTTTT31700.036.593061
GGCCTAT1350.035.5555578
CATACGA2150.035.34883518
GCCGATT800.035.0000049
GCGATAT1500.034.6666689
AGGGTAC3950.034.4303786
CTCGTAC701.8189894E-1234.28571329
AATGCGG2450.034.2857132
TCGTACG701.8189894E-1234.28571330
ACGGTTA701.8189894E-1234.28571334
GCACTTA8450.033.84615336
TAGGGCC3700.033.5135155
ACACGAC3350.033.43283526
Produced by FastQC (version 0.11.5)