##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1060441
Sequences flagged as poor quality	0
Sequence length	46
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.829817028953048	31.0	31.0	34.0	30.0	34.0
2	31.97620423955694	33.0	31.0	34.0	30.0	34.0
3	31.921609971700452	33.0	31.0	34.0	30.0	34.0
4	35.67796416773776	37.0	35.0	37.0	33.0	37.0
5	35.49424248968118	37.0	35.0	37.0	33.0	37.0
6	35.48953218519465	37.0	35.0	37.0	33.0	37.0
7	35.80072913061641	37.0	35.0	37.0	35.0	37.0
8	35.61422653405517	37.0	35.0	37.0	35.0	37.0
9	37.400715362759456	39.0	37.0	39.0	35.0	39.0
10	36.850101985871916	39.0	37.0	39.0	32.0	39.0
11	36.781683280823735	39.0	37.0	39.0	32.0	39.0
12	36.746191442994	39.0	35.0	39.0	32.0	39.0
13	35.120150956064506	39.0	35.0	39.0	30.0	39.0
14	36.89854409627693	40.0	36.0	41.0	30.0	41.0
15	37.5145689387717	40.0	36.0	41.0	32.0	41.0
16	37.70622410864914	40.0	36.0	41.0	33.0	41.0
17	37.745412521771605	39.0	36.0	41.0	33.0	41.0
18	37.77346688783251	39.0	37.0	41.0	33.0	41.0
19	37.73054040724566	39.0	36.0	41.0	33.0	41.0
20	37.67295776002625	39.0	36.0	41.0	33.0	41.0
21	37.564668850035034	39.0	36.0	41.0	33.0	41.0
22	37.5492516792542	39.0	36.0	41.0	33.0	41.0
23	37.46161832671502	39.0	36.0	41.0	32.0	41.0
24	37.391409800262345	39.0	35.0	41.0	32.0	41.0
25	37.194568108928266	39.0	35.0	41.0	32.0	41.0
26	37.15182268509045	39.0	35.0	41.0	32.0	41.0
27	37.11620259873015	39.0	35.0	41.0	32.0	41.0
28	36.978788070246246	39.0	35.0	41.0	31.0	41.0
29	36.924653045289645	39.0	35.0	41.0	31.0	41.0
30	36.78992796393199	39.0	35.0	41.0	31.0	41.0
31	36.72598569840284	39.0	35.0	41.0	31.0	41.0
32	36.58732546176544	39.0	35.0	40.0	30.0	41.0
33	36.465512932827004	39.0	35.0	40.0	30.0	41.0
34	36.32970151097515	39.0	35.0	40.0	30.0	41.0
35	36.208826327914515	39.0	35.0	40.0	30.0	41.0
36	36.07570718220061	38.0	35.0	40.0	29.0	41.0
37	35.992837885370335	38.0	35.0	40.0	29.0	41.0
38	35.86770598269965	38.0	35.0	40.0	29.0	41.0
39	35.74816420715533	38.0	35.0	40.0	28.0	41.0
40	35.64249024698215	38.0	35.0	40.0	28.0	41.0
41	35.51561567310204	38.0	34.0	40.0	27.0	41.0
42	35.43853453421737	38.0	34.0	40.0	27.0	41.0
43	35.37077876091173	38.0	34.0	40.0	27.0	41.0
44	35.2746696893085	38.0	34.0	40.0	27.0	41.0
45	35.1785785347794	38.0	34.0	40.0	26.0	41.0
46	34.19242371805692	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	32.0
10	57.0
11	37.0
12	27.0
13	30.0
14	25.0
15	46.0
16	73.0
17	182.0
18	273.0
19	547.0
20	993.0
21	1581.0
22	2456.0
23	3658.0
24	4959.0
25	6979.0
26	9426.0
27	12575.0
28	16018.0
29	18939.0
30	22821.0
31	28013.0
32	35554.0
33	48480.0
34	71578.0
35	89932.0
36	105276.0
37	146592.0
38	214545.0
39	218727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.46343643823654	20.016860909753582	18.5369105871991	27.982792064810774
2	24.654554095890294	27.435189699379787	23.703817562693256	24.206438642036662
3	28.227312976393783	26.269165375537156	24.038018145281068	21.465503502787993
4	25.357280603069853	27.221693616146492	23.25730521547168	24.163720565311976
5	21.954828227124377	32.04770468135427	21.44956673685759	24.547900354663767
6	21.07481698651787	39.381917522992794	21.64505144557783	17.898214044911505
7	82.73444727240836	5.373141928688159	8.060420145958144	3.8319906529453314
8	82.4969988900844	5.335704673810236	7.829478490552516	4.337817945552841
9	76.4858205218395	7.050368667375177	10.39143148935207	6.072379321433253
10	36.817324113269855	26.526416839786464	17.512714050098026	19.14354499684565
11	29.85059989193175	27.34711313500704	23.373388995710275	19.428897977350932
12	25.034396067296534	24.08196212707732	29.020002055748506	21.863639749877645
13	22.879349251867858	26.66437831053307	30.381228187141012	20.075044250458063
14	22.026025021665514	27.230369252037594	28.557270041426165	22.186335684870727
15	21.394778210197455	26.027284874877527	31.00511956817965	21.572817346745364
16	22.81013276551925	24.592032937240262	30.579541907564874	22.01829238967562
17	22.534492725196404	25.067495504228898	28.097932841148165	24.30007892942653
18	23.285878233678254	24.49056571747037	28.529828627901033	23.693727420950342
19	25.28664961086944	26.054254786452052	26.621283032247906	22.037812570430603
20	27.117963187013704	24.646444262339916	26.38798386708926	21.847608683557123
21	27.17294031445408	26.180240107653326	25.757585759132283	20.889233818760307
22	25.28231179292389	24.074700997038025	26.181183111554535	24.461804098483555
23	24.115533065960292	26.49756092040953	26.349037805969406	23.037868207660775
24	23.47438471352956	25.40179038720683	27.844359092113564	23.279465807150043
25	24.393342015255918	26.174204882685597	26.335835751352505	23.09661735070598
26	23.261265831856743	26.396753803370483	26.460029365141484	23.881950999631286
27	24.04754248468326	25.634052248074152	26.675411456177194	23.642993811065395
28	22.98072217124762	26.650893354745808	27.32900746010386	23.03937701390271
29	24.26283027532885	26.04322164080793	26.24983379556241	23.44411428830081
30	24.65662870447295	24.697649374175462	26.6340135849142	24.011708336437387
31	25.597652297487556	25.50599231829022	24.79911659394535	24.097238790276876
32	25.845850924285273	25.409617319586854	25.97230774743715	22.772224008690724
33	24.448602043866654	25.805113155753123	24.64201214400424	25.104272656375983
34	24.36090268105439	25.844813619993946	26.293023374237702	23.501260324713964
35	24.031322817582495	25.151988653777064	26.6681503261379	24.148538202502543
36	24.950563020479215	27.6510432923661	24.290271688853977	23.108121998300707
37	23.68552328700984	27.62633659015447	26.768203040055976	21.91993708277971
38	22.949791643288027	27.993542309284535	26.211736437953643	22.844929609473795
39	23.517102790254242	26.292646172677216	26.24049805694046	23.94975298012808
40	23.967481453470775	25.754756747428665	28.242118137642734	22.035643661457826
41	21.923237596433935	26.724636259820205	27.69083805699704	23.661288086748815
42	23.32774760689185	27.02177678909058	26.21390534692642	23.436570257091155
43	23.09133652885922	25.3898142376615	27.57465997636832	23.944189257110956
44	23.738425805867557	25.631411837150768	26.712000007544027	23.918162349437637
45	23.70560927010555	24.50367347169715	27.05016120651691	24.740556051680386
46	23.76068069793605	26.046522154462153	26.350923813771814	23.841873333829984
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3332.0
1	3466.0
2	3600.0
3	7914.5
4	12229.0
5	8348.5
6	4468.0
7	4468.0
8	4032.0
9	3596.0
10	3628.5
11	3661.0
12	3559.5
13	3458.0
14	3399.0
15	3340.0
16	3296.0
17	3252.0
18	3336.0
19	3420.0
20	3420.0
21	3430.0
22	3440.0
23	4144.0
24	4848.0
25	6097.5
26	7347.0
27	8158.0
28	8969.0
29	11291.5
30	13614.0
31	13614.0
32	15983.5
33	18353.0
34	20959.5
35	23566.0
36	26448.5
37	29331.0
38	32962.5
39	36594.0
40	40789.5
41	44985.0
42	49476.0
43	53967.0
44	53967.0
45	60145.5
46	66324.0
47	75226.0
48	84128.0
49	86756.0
50	89384.0
51	86007.5
52	82631.0
53	77122.5
54	71614.0
55	68718.5
56	65823.0
57	65823.0
58	65162.5
59	64502.0
60	61069.0
61	57636.0
62	54222.0
63	50808.0
64	45232.0
65	39656.0
66	35061.0
67	30466.0
68	26826.5
69	23187.0
70	23187.0
71	19700.5
72	16214.0
73	13573.0
74	10932.0
75	9473.0
76	8014.0
77	6331.5
78	4649.0
79	3773.0
80	2897.0
81	2897.0
82	2144.0
83	1391.0
84	947.5
85	504.0
86	342.5
87	181.0
88	131.5
89	82.0
90	55.5
91	29.0
92	22.0
93	15.0
94	15.0
95	9.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1060441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.251629573703326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1117068813563	22.11748274075967
2	9.210669766633645	5.572755398118189
3	3.416878309690847	3.1009841076956715
4	1.9373849833877508	2.3443621143640643
5	1.279785494935293	1.9357798363290526
6	1.0332145012046383	1.8753853416372819
7	0.8228585273886264	1.7424967953486619
8	0.6846588715016284	1.6569637252013607
9	0.6023360009928477	1.6399481022847284
>10	7.652977921730381	49.79550719592698
>50	0.20272042705537555	3.7543586847659154
>100	0.039245902714574364	2.338700478137376
>500	0.004326319984122914	0.9107255089564996
>1k	9.270685680263386E-4	0.5655809206720203
>5k	3.090228560087796E-4	0.6489690498025067
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6942	0.6546333082179961	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3026	0.28535298050528035	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	1936	0.18256555527370216	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTA	1088	0.10259882445133675	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0622382574796712	0.0
2	0.0	0.0	0.0	0.3120399909094424	0.0
3	0.0	0.0	0.0	0.4617890104211361	0.0
4	0.0	0.0	0.0	0.6701928725879139	0.0
5	0.0	0.0	0.0	1.264473931128653	0.0
6	0.0	0.0	0.0	2.065461444813997	0.0
7	0.0	0.0	0.0	2.598635850556514	0.0
8	0.0	0.0	0.0	3.544657364247516	0.0
9	0.0	0.0	0.0	3.9836256802594394	0.0
10	0.0	0.0	0.0	4.757266080809776	0.0
11	0.0	0.0	0.0	5.417463112044895	0.0
12	0.0	0.0	0.0	6.041731694644021	0.0
13	0.0	0.0	0.0	6.318786240818678	0.0
14	0.0	0.0	0.0	6.445997467091521	0.0
15	0.0	0.0	0.0	6.607251134197942	0.0
16	0.0	0.0	0.0	6.924100445003541	0.0
17	0.0	0.0	0.0	7.2794243149783915	0.0
18	0.0	0.0	0.0	7.711791603681864	9.430039012071393E-5
19	0.0	0.0	0.0	7.9149146440018825	1.8860078024142787E-4
20	0.0	0.0	0.0	8.121526798756367	1.8860078024142787E-4
21	0.0	0.0	0.0	8.36510470643817	1.8860078024142787E-4
22	0.0	0.0	0.0	8.621696067956632	1.8860078024142787E-4
23	0.0	0.0	0.0	8.891678084872238	1.8860078024142787E-4
24	0.0	0.0	0.0	9.119036325453278	1.8860078024142787E-4
25	0.0	0.0	0.0	9.319141753289433	1.8860078024142787E-4
26	0.0	0.0	0.0	9.503970517926033	1.8860078024142787E-4
27	9.430039012071393E-5	0.0	0.0	9.705301850833758	1.8860078024142787E-4
28	9.430039012071393E-5	0.0	0.0	9.910782400906793	1.8860078024142787E-4
29	9.430039012071393E-5	0.0	0.0	10.124844286480814	1.8860078024142787E-4
30	9.430039012071393E-5	0.0	0.0	10.41519518766249	1.8860078024142787E-4
31	9.430039012071393E-5	0.0	0.0	10.648400052431017	1.8860078024142787E-4
32	9.430039012071393E-5	0.0	0.0	10.874909589500971	1.8860078024142787E-4
33	9.430039012071393E-5	0.0	0.0	11.09915591720803	1.8860078024142787E-4
34	9.430039012071393E-5	0.0	0.0	11.320007430870742	4.7150195060356964E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGG	40	1.9281288E-8	40.000004	2
ACGATTG	25	7.794245E-5	40.0	1
GTCAACG	25	7.794245E-5	40.0	1
TAATGCG	75	0.0	40.0	1
ATTACCG	35	3.056084E-7	39.999996	1
CGACAGG	255	0.0	37.64706	2
CTATACG	65	0.0	36.923077	1
CGTTTTT	3170	0.0	36.59306	1
GGCCTAT	135	0.0	35.555557	8
CATACGA	215	0.0	35.348835	18
GCCGATT	80	0.0	35.000004	9
GCGATAT	150	0.0	34.666668	9
AGGGTAC	395	0.0	34.430378	6
CTCGTAC	70	1.8189894E-12	34.285713	29
AATGCGG	245	0.0	34.285713	2
TCGTACG	70	1.8189894E-12	34.285713	30
ACGGTTA	70	1.8189894E-12	34.285713	34
GCACTTA	845	0.0	33.846153	36
TAGGGCC	370	0.0	33.513515	5
ACACGAC	335	0.0	33.432835	26
>>END_MODULE