##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553745_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488522
Sequences flagged as poor quality	0
Sequence length	46
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810610371692576	31.0	31.0	34.0	30.0	34.0
2	31.948960333413847	33.0	31.0	34.0	30.0	34.0
3	31.910751204654037	33.0	31.0	34.0	30.0	34.0
4	35.65881372793856	37.0	35.0	37.0	33.0	37.0
5	35.46865647811153	37.0	35.0	37.0	33.0	37.0
6	35.46592783948318	37.0	35.0	37.0	33.0	37.0
7	35.72602052722293	37.0	35.0	37.0	35.0	37.0
8	35.511020998030794	37.0	35.0	37.0	33.0	37.0
9	37.30145418220674	39.0	37.0	39.0	34.0	39.0
10	36.77119147141787	39.0	37.0	39.0	32.0	39.0
11	36.70633052349741	39.0	37.0	39.0	32.0	39.0
12	36.68909076766246	39.0	35.0	39.0	32.0	39.0
13	35.15330527591388	39.0	35.0	39.0	30.0	39.0
14	36.882068770700194	39.0	36.0	41.0	30.0	41.0
15	37.45892099025223	39.0	36.0	41.0	32.0	41.0
16	37.65140362153598	39.0	36.0	41.0	33.0	41.0
17	37.65988225709385	39.0	36.0	41.0	33.0	41.0
18	37.69988864370489	39.0	36.0	41.0	33.0	41.0
19	37.64091279410139	39.0	36.0	41.0	33.0	41.0
20	37.56997023675495	39.0	36.0	41.0	32.0	41.0
21	37.46713556400735	39.0	36.0	41.0	32.0	41.0
22	37.4382689008888	39.0	36.0	41.0	32.0	41.0
23	37.363428873213486	39.0	35.0	41.0	32.0	41.0
24	37.28944448765869	39.0	35.0	41.0	32.0	41.0
25	37.09609802629155	39.0	35.0	41.0	31.0	41.0
26	37.04483523771703	39.0	35.0	41.0	31.0	41.0
27	37.0027982363128	39.0	35.0	41.0	31.0	41.0
28	36.86411256811362	39.0	35.0	41.0	31.0	41.0
29	36.80628712729416	39.0	35.0	40.0	31.0	41.0
30	36.663523853582845	39.0	35.0	40.0	31.0	41.0
31	36.599203311212186	39.0	35.0	40.0	30.0	41.0
32	36.461320063374835	39.0	35.0	40.0	30.0	41.0
33	36.308381198799644	39.0	35.0	40.0	30.0	41.0
34	36.16474181306062	38.0	35.0	40.0	30.0	41.0
35	36.011176569325436	38.0	35.0	40.0	29.0	41.0
36	35.859465899181615	38.0	35.0	40.0	29.0	41.0
37	35.757278075501205	38.0	35.0	40.0	29.0	41.0
38	35.69921723074908	38.0	35.0	40.0	28.0	41.0
39	35.52578389509582	38.0	34.0	40.0	27.0	41.0
40	35.41775191291283	38.0	34.0	40.0	27.0	41.0
41	35.286726083983936	38.0	34.0	40.0	26.0	41.0
42	35.23916425462927	38.0	34.0	40.0	27.0	41.0
43	35.192261556286105	38.0	34.0	40.0	27.0	41.0
44	35.14396281027262	38.0	34.0	40.0	27.0	41.0
45	35.058075173687165	37.0	34.0	40.0	26.0	41.0
46	34.068920130516126	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	17.0
10	19.0
11	16.0
12	15.0
13	8.0
14	19.0
15	21.0
16	47.0
17	79.0
18	143.0
19	306.0
20	489.0
21	849.0
22	1367.0
23	1965.0
24	2671.0
25	3446.0
26	4596.0
27	5861.0
28	7088.0
29	8784.0
30	10525.0
31	13312.0
32	17112.0
33	23120.0
34	34472.0
35	43046.0
36	49509.0
37	68509.0
38	96205.0
39	94901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.731836027855447	20.51985376298304	19.647016920425283	28.10129328873623
2	22.1355435374456	29.828544057381244	24.516603141721355	23.5193092634518
3	27.732425561182506	27.887587457678464	24.08960087774962	20.29038610338941
4	24.90450788296126	27.899460003848343	23.477960050929127	23.71807206226127
5	22.63971735152153	32.01718653407625	20.764878552040646	24.578217562361573
6	20.263365825899346	39.73597913707059	21.261273801384583	18.739381235645478
7	82.04563970506958	5.504358043240632	8.442813220284858	4.007189031404931
8	82.50191393632221	5.934021395146995	7.212367099127573	4.35169756940322
9	76.39881110779044	6.880549903586737	9.85830730243469	6.862331686188135
10	38.1102591080852	25.888701020629572	17.230134978568007	18.770904892717216
11	30.83136481059195	26.436680436090903	22.611673578671994	20.120281174645154
12	25.146257486868556	23.07715926815988	28.689393722288862	23.087189522682706
13	22.860587650095596	25.859633752420567	30.1304751884255	21.149303409058344
14	22.01579458038737	26.375066015450688	28.706588444327995	22.90255095983395
15	20.96077556384359	25.018525266006442	31.333082235805144	22.68761693434482
16	23.16129058670848	23.910898587985802	29.33521929411572	23.592591531189996
17	22.75127834570398	24.71229545445241	27.343087926439342	25.19333827340427
18	23.511530698719813	24.291434162637508	27.44830324939307	24.748731889249616
19	25.055780497091224	26.117145184863734	25.668854217414978	23.158220100630064
20	27.718096626149897	24.354481476780983	25.482578061991067	22.44484383507805
21	26.90278022279447	26.359304186914816	25.100814292907998	21.637101297382717
22	25.436930168958614	24.00424136476965	25.1153479270125	25.443480539259234
23	25.26109366620132	25.82135502597631	24.83736658737989	24.080184720442478
24	23.59177273490242	24.87257482774573	27.66958294611092	23.86606949124093
25	24.469727054257536	26.65611784116171	25.200707439992463	23.673447664588288
26	23.181760493897922	26.32184425675814	25.69689799026451	24.799497259079427
27	24.47402573476732	25.315748318397123	25.11555262608439	25.094673320751166
28	22.846668113206775	26.87268945922599	26.61538272585472	23.66525970171251
29	24.04129189678254	25.345839081965604	25.216878666672127	25.395990354579734
30	24.080594118586266	25.987570672354572	25.16795558848936	24.7638796205698
31	25.320456397050695	25.982862593701	23.21942512312649	25.477255886121814
32	26.556429393149134	25.325369174776164	23.685524909830058	24.43267652224465
33	25.04841133050303	25.99432574172709	23.57703440172602	25.38022852604386
34	24.72498679690986	25.446346326265758	24.86745735094837	24.96120952587601
35	26.06269523173982	24.52090182223114	25.100404894764207	24.315998051264838
36	25.49813519145504	27.06060320722506	23.718276761333165	23.722984839986736
37	25.20746250936498	26.763789552978167	25.475004196330975	22.553743741325878
38	25.559544913023363	25.487490839716532	25.435701974527248	23.517262272732857
39	25.328439660854578	24.682614089027723	26.317136178104565	23.671810072013134
40	25.629142597467464	24.172913400010646	27.719324820581264	22.47861918194063
41	22.395306659679605	24.782507236112192	27.770499588554863	25.051686515653337
42	24.005264860129124	25.244308342305978	26.74864182165798	24.00178497590692
43	23.904348217685182	24.28038041275521	26.86450149635022	24.950769873209396
44	24.449257147068096	24.832453809654428	26.22891087811808	24.489378165159398
45	23.88572060214279	24.403199855891852	26.046728704132054	25.6643508378333
46	24.7346076532889	25.318614105403647	25.81419055846001	24.132587682847443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1761.0
1	1819.0
2	1877.0
3	3132.5
4	4388.0
5	3052.5
6	1717.0
7	1717.0
8	1588.5
9	1460.0
10	1457.5
11	1455.0
12	1424.5
13	1394.0
14	1399.0
15	1404.0
16	1310.5
17	1217.0
18	1276.0
19	1335.0
20	1335.0
21	1295.5
22	1256.0
23	1518.0
24	1780.0
25	2456.5
26	3133.0
27	3524.0
28	3915.0
29	4999.5
30	6084.0
31	6084.0
32	6855.0
33	7626.0
34	8604.0
35	9582.0
36	10676.0
37	11770.0
38	13553.0
39	15336.0
40	16653.5
41	17971.0
42	20311.0
43	22651.0
44	22651.0
45	25603.0
46	28555.0
47	30574.0
48	32593.0
49	38243.0
50	43893.0
51	42776.0
52	41659.0
53	39234.0
54	36809.0
55	34765.0
56	32721.0
57	32721.0
58	31777.0
59	30833.0
60	29429.5
61	28026.0
62	26406.0
63	24786.0
64	22140.0
65	19494.0
66	17610.5
67	15727.0
68	13584.0
69	11441.0
70	11441.0
71	9965.5
72	8490.0
73	7038.5
74	5587.0
75	4733.0
76	3879.0
77	3077.5
78	2276.0
79	1868.5
80	1461.0
81	1461.0
82	1062.5
83	664.0
84	489.5
85	315.0
86	218.0
87	121.0
88	84.0
89	47.0
90	34.5
91	22.0
92	16.0
93	10.0
94	10.0
95	5.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	488522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.02292082884061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.72050689874449	24.061182071914597
2	8.663881539806233	5.895411113985624
3	3.8359219530023303	3.9152780673782988
4	2.504213095346287	3.4080257552605047
5	1.7942624847991338	3.0523025233239887
6	1.4915295269867295	3.044771460632855
7	1.2260542199366986	2.919976195974056
8	1.1120197607579152	3.026732822429826
9	0.9520449478353008	2.9152214897128323
>10	7.615930067489833	42.78617373319351
>50	0.05212067721287897	1.1810987300849172
>100	0.02666639299263575	1.764224988583546
>500	0.002424217544785068	0.5823014688826477
>1k	0.002424217544785068	1.447299578642813
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2407	0.49271066604984015	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG	1896	0.3881094403117976	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1610	0.32956550574999693	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTA	1106	0.22639717351521527	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCG	976	0.19978629416894222	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	698	0.1428799521822968	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	613	0.12548053107127213	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGC	537	0.10992340160729712	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0469907189440804E-4	0.0	0.0	0.07471516124145892	0.0
2	2.0469907189440804E-4	0.0	0.0	0.413901523370493	0.0
3	2.0469907189440804E-4	0.0	0.0	0.5962883964284106	0.0
4	2.0469907189440804E-4	0.0	0.0	0.8484776530023213	0.0
5	2.0469907189440804E-4	0.0	0.0	1.6603141721355434	0.0
6	2.0469907189440804E-4	0.0	0.0	2.5585336996082058	0.0
7	2.0469907189440804E-4	0.0	0.0	3.165057049631337	0.0
8	2.0469907189440804E-4	0.0	0.0	4.178522154580551	0.0
9	2.0469907189440804E-4	0.0	0.0	4.635001084905081	0.0
10	2.0469907189440804E-4	0.0	0.0	5.554918713998551	0.0
11	2.0469907189440804E-4	0.0	0.0	6.207294656126029	0.0
12	2.0469907189440804E-4	0.0	0.0	7.039805781520586	0.0
13	2.0469907189440804E-4	0.0	0.0	7.36036452810723	0.0
14	2.0469907189440804E-4	0.0	0.0	7.510613646877725	0.0
15	2.0469907189440804E-4	0.0	0.0	7.686245450563127	0.0
16	2.0469907189440804E-4	0.0	0.0	7.971391257712037	0.0
17	2.0469907189440804E-4	0.0	0.0	8.27516468040334	0.0
18	2.0469907189440804E-4	0.0	0.0	8.64341831074138	0.0
19	2.0469907189440804E-4	0.0	0.0	8.846684489132526	0.0
20	2.0469907189440804E-4	0.0	0.0	9.038282820425692	0.0
21	2.0469907189440804E-4	0.0	0.0	9.251988651483455	0.0
22	2.0469907189440804E-4	0.0	0.0	9.466513278828794	0.0
23	2.0469907189440804E-4	0.0	0.0	9.71338035953345	0.0
24	2.0469907189440804E-4	0.0	0.0	9.905797487114194	0.0
25	2.0469907189440804E-4	0.0	0.0	10.078972901936863	0.0
26	2.0469907189440804E-4	0.0	0.0	10.24273215945239	0.0
27	2.0469907189440804E-4	0.0	0.0	10.435763384248816	0.0
28	2.0469907189440804E-4	0.0	0.0	10.599522641764342	0.0
29	2.0469907189440804E-4	0.0	0.0	10.799513635005178	0.0
30	2.0469907189440804E-4	0.0	0.0	11.059890854454865	0.0
31	4.093981437888161E-4	0.0	0.0	11.24575761173499	0.0
32	4.093981437888161E-4	0.0	0.0	11.445339206832037	0.0
33	4.093981437888161E-4	0.0	0.0	11.631820061327842	0.0
34	4.093981437888161E-4	0.0	0.0	11.823008994477219	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	20	0.00125506	40.000004	38
ATGCTAC	20	0.00125506	40.000004	25
CTAAACG	40	1.9244908E-8	40.000004	1
GTATGCG	20	0.00125506	40.000004	1
ACATGCG	20	0.00125506	40.000004	1
ATTAGTC	50	7.8216544E-11	40.0	31
TATGCGG	45	1.2187229E-9	40.0	2
GTTAACG	25	7.788067E-5	40.0	1
TCGTAAG	30	4.8617603E-6	40.0	1
TTAGTCA	45	1.2187229E-9	40.0	32
TTAAACG	25	7.788067E-5	40.0	1
TTGCCGC	25	7.788067E-5	40.0	19
TCAAGCG	190	0.0	38.94737	17
GCGAGAC	190	0.0	37.894737	21
GCGATAA	95	0.0	37.894737	9
TAAACGG	85	0.0	37.647057	2
CACGACC	205	0.0	37.07317	27
CTAACGG	60	1.4551915E-11	36.666664	2
ACGGGAC	165	0.0	36.363636	5
TCTACGG	55	2.1645974E-10	36.363636	2
>>END_MODULE