FastQCFastQC Report
Sat 18 Jun 2016
SRR3553744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061849
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50890.47925835029274405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20680.1947546214198064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA600.040.00000431
CGCGTCA304.8671627E-640.00000439
CATACCG200.001255725540.01
ACGCATT1400.038.5714317
CGTAAGG1900.035.789472
ACGGGAC4050.035.5555575
TCTACGA455.4418706E-835.55555322
CGTTTTT24150.035.527951
TTAACGG800.035.02
GCAAACG1150.034.7826041
TACGCAT1500.034.66666816
AAGGGCG7150.034.4055945
AACACCG351.4009673E-534.2857131
CATACGA2050.034.14634318
GCGGGAT3950.033.924055
TATGCGG1250.033.62
CGAGGGT2750.033.4545444
CTTACTG13000.033.38461739
CTTAACG302.2781442E-433.3333361
ACCGCGT302.2781442E-433.33333625
Produced by FastQC (version 0.11.5)