##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1061849
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76647809622649	31.0	31.0	34.0	30.0	34.0
2	31.934818415801118	33.0	31.0	34.0	30.0	34.0
3	31.855086740205056	33.0	31.0	34.0	30.0	34.0
4	35.632856460758546	37.0	35.0	37.0	33.0	37.0
5	33.72819864217982	37.0	35.0	37.0	30.0	37.0
6	34.56643458721532	37.0	35.0	37.0	30.0	37.0
7	35.501839715439765	37.0	35.0	37.0	32.0	37.0
8	35.60556256115512	37.0	35.0	37.0	35.0	37.0
9	37.39296453638888	39.0	37.0	39.0	35.0	39.0
10	36.77342917872504	39.0	37.0	39.0	32.0	39.0
11	36.50221264982121	39.0	35.0	39.0	32.0	39.0
12	36.673336792707815	39.0	35.0	39.0	32.0	39.0
13	33.03093000982249	38.0	35.0	39.0	10.0	39.0
14	35.3005681598796	39.0	35.0	41.0	17.0	41.0
15	36.74185406776293	39.0	35.0	41.0	30.0	41.0
16	37.38601062862987	39.0	36.0	41.0	32.0	41.0
17	37.58153466264977	39.0	36.0	41.0	33.0	41.0
18	37.674229575014905	39.0	36.0	41.0	33.0	41.0
19	37.66808840051646	39.0	36.0	41.0	33.0	41.0
20	37.65750780007327	39.0	36.0	41.0	33.0	41.0
21	37.57398274142557	39.0	36.0	41.0	33.0	41.0
22	37.57329808663944	39.0	36.0	41.0	33.0	41.0
23	37.455503560299064	39.0	36.0	41.0	32.0	41.0
24	37.4196623060341	39.0	36.0	41.0	32.0	41.0
25	37.21661554514813	39.0	35.0	41.0	32.0	41.0
26	37.185359688618625	39.0	35.0	41.0	32.0	41.0
27	36.77093259022705	39.0	35.0	41.0	31.0	41.0
28	36.83668864405391	39.0	35.0	40.0	31.0	41.0
29	36.88721748572537	39.0	35.0	40.0	31.0	41.0
30	36.40574036421374	39.0	35.0	40.0	30.0	41.0
31	36.49206525598272	39.0	35.0	40.0	30.0	41.0
32	36.434746371659244	39.0	35.0	40.0	30.0	41.0
33	36.36689114930654	39.0	35.0	40.0	30.0	41.0
34	36.33187957986493	39.0	35.0	40.0	30.0	41.0
35	36.24635423680768	39.0	35.0	40.0	30.0	41.0
36	36.118356753173	39.0	35.0	40.0	30.0	41.0
37	36.10024683358933	39.0	35.0	40.0	30.0	41.0
38	35.98893157124977	38.0	35.0	40.0	29.0	41.0
39	35.93823133044341	38.0	35.0	40.0	29.0	41.0
40	35.84234669901276	38.0	35.0	40.0	29.0	41.0
41	35.665799939539426	38.0	35.0	40.0	28.0	41.0
42	35.59006506574852	38.0	34.0	40.0	28.0	41.0
43	35.526256558135856	38.0	34.0	40.0	28.0	41.0
44	35.45308890435457	38.0	34.0	40.0	27.0	41.0
45	35.372646204874705	38.0	34.0	40.0	27.0	41.0
46	34.28810122719897	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	32.0
10	41.0
11	38.0
12	29.0
13	27.0
14	23.0
15	31.0
16	73.0
17	141.0
18	294.0
19	494.0
20	875.0
21	1517.0
22	2294.0
23	3365.0
24	5031.0
25	6876.0
26	9678.0
27	12871.0
28	16413.0
29	19908.0
30	24293.0
31	31367.0
32	40507.0
33	54633.0
34	76768.0
35	96387.0
36	114683.0
37	155651.0
38	197887.0
39	189617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.560186994572675	23.011087263819995	22.216247319534133	20.212478422073197
2	27.81996310209832	26.268518405159302	23.285325879668388	22.626192613073986
3	29.24144581762567	25.889085924646537	23.396358615961404	21.473109641766392
4	25.477257124129704	27.300491877847037	22.90438659357404	24.31786440444922
5	20.835636705407264	34.65464486946826	20.363064804882804	24.146653620241672
6	20.271432190452693	39.94202565524853	22.04456565858234	17.741976495716436
7	83.27766000627209	5.052978342495025	7.741496201437305	3.9278654497955925
8	83.39961708303159	4.71743157454591	7.661541330264472	4.221410012158038
9	76.84538950453407	6.811702982250773	10.467119147826104	5.875788365389052
10	35.61664605796116	26.047771387457164	18.813503614920766	19.52207893966091
11	28.636651727317158	27.16356092062054	24.147407023032468	20.052380329029834
12	24.273696165839024	24.604722517043385	30.07602775912583	21.045553557991767
13	21.58150546829163	30.80758186898514	29.390713745551395	18.22019891717184
14	22.263805870702896	27.663161146264674	29.114968324121413	20.958064658911013
15	21.440995847808868	26.57242225589514	31.013355006220284	20.973226890075708
16	23.439961802478507	24.676860834261745	30.601432030354598	21.28174533290515
17	23.46802605643552	25.404836280864796	27.601758818815103	23.52537884388458
18	24.22406575699558	24.998187124534656	28.17858283051545	22.599164287954313
19	25.201323352002024	27.265270297377498	26.35563060284466	21.177775747775815
20	26.519495709841983	25.30246767666589	26.465815760998034	21.712220852494095
21	27.26112658202814	25.732001442766343	27.022486248044686	19.98438572716083
22	25.160262899903845	23.666641867158138	27.596484999279557	23.576610233658457
23	23.353414656886244	26.79495860522541	27.2456818248169	22.605944913071443
24	23.45615996248054	26.135636987933314	28.35826939611941	22.049933653466734
25	24.645500443095017	26.111904800023357	26.69532108614313	22.547273670738495
26	22.377004640019436	26.7474942294055	27.111105251311628	23.764395879263436
27	22.69070272703558	26.25015421213374	27.94474543932329	23.11439762150739
28	23.08708677034117	26.14627880235325	28.164362352839245	22.60227207446633
29	23.39786542154299	26.823117034531275	26.334629500051328	23.44438804387441
30	23.24031006291855	26.21389670282686	27.584053853231488	22.9617393810231
31	25.1795688464179	26.330579960050816	25.756769559513643	22.733081634017644
32	24.155976979777726	26.709824089865887	25.877314006040407	23.256884924315983
33	23.63594070343335	26.733744628473538	25.343999005508316	24.286315662584794
34	23.528769156443147	26.53428123961128	26.35958596749632	23.57736363644925
35	23.419619927127115	26.078566726530795	26.43680975355253	24.06500359278956
36	24.326999413287574	27.996636056539113	25.265927641312462	22.410436888860847
37	23.643945608085517	28.147787491441818	25.796982433472177	22.411284467000485
38	23.155175547559022	27.896621836061435	26.491337280536122	22.45686533584342
39	23.48177565736748	26.35996266889172	25.596012239028337	24.56224943471247
40	24.06180163092869	25.279394716197878	28.55368324498116	22.10512040789227
41	22.039197663697944	26.205044220035052	27.779750228139783	23.97600788812722
42	22.477960613985605	27.620217187189517	26.634295460088957	23.26752673873592
43	22.527967724224442	25.4678395892448	28.13601557283569	23.868177113695076
44	23.686230339718737	25.717121737648196	27.225622475512058	23.371025447121013
45	23.614657074593467	25.201040825955477	26.715192084750278	24.46911001470077
46	23.95378250579885	25.81026115765989	26.615083688923757	23.620872647617507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2935.0
1	2892.0
2	2849.0
3	6547.5
4	10246.0
5	7073.5
6	3901.0
7	3901.0
8	3658.0
9	3415.0
10	3405.0
11	3395.0
12	3361.5
13	3328.0
14	3330.5
15	3333.0
16	3224.0
17	3115.0
18	3081.5
19	3048.0
20	3048.0
21	3284.5
22	3521.0
23	4295.5
24	5070.0
25	6259.0
26	7448.0
27	8472.0
28	9496.0
29	11951.0
30	14406.0
31	14406.0
32	16986.5
33	19567.0
34	21903.0
35	24239.0
36	27878.5
37	31518.0
38	35271.0
39	39024.0
40	44109.5
41	49195.0
42	53995.5
43	58796.0
44	58796.0
45	67145.5
46	75495.0
47	82161.0
48	88827.0
49	92403.5
50	95980.0
51	93296.5
52	90613.0
53	83357.0
54	76101.0
55	72203.0
56	68305.0
57	68305.0
58	64468.5
59	60632.0
60	56261.5
61	51891.0
62	47191.0
63	42491.0
64	37720.5
65	32950.0
66	29383.5
67	25817.0
68	22319.0
69	18821.0
70	18821.0
71	15836.5
72	12852.0
73	10428.0
74	8004.0
75	6690.0
76	5376.0
77	4181.0
78	2986.0
79	2244.0
80	1502.0
81	1502.0
82	1163.0
83	824.0
84	542.5
85	261.0
86	205.5
87	150.0
88	102.0
89	54.0
90	50.0
91	46.0
92	29.5
93	13.0
94	13.0
95	11.5
96	10.0
97	6.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1061849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.46884776338391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.01487153230293	26.262993866112055
2	9.068667857460486	6.614477350208389
3	3.91038626466179	4.278218441459349
4	2.4828694347980123	3.6218954973603106
5	1.8413247560575712	3.357549610580679
6	1.4099787492925007	3.0852180214532794
7	1.1795341815839067	3.011137674992379
8	0.9691859211587538	2.827607505052287
9	0.8519683661822856	2.7963274180968622
>10	6.1797383489323305	38.88806851198465
>50	0.057567313369672045	1.3740935878237877
>100	0.029438873740184557	2.1203635602474953
>500	0.003154165030295088	0.7836334568647416
>1k	0.0010513883434316962	0.49059767978394464
>5k	2.6284708585792404E-4	0.48781781797977614
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5089	0.47925835029274405	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2068	0.1947546214198064	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.417534884903597E-5	0.0	0.0	0.11272789257229607	0.0
2	9.417534884903597E-5	0.0	0.0	0.42115216005288886	0.0
3	9.417534884903597E-5	0.0	0.0	0.6300330838000506	0.0
4	9.417534884903597E-5	0.0	0.0	0.8602918117359436	0.0
5	9.417534884903597E-5	0.0	0.0	1.3812698415688107	0.0
6	9.417534884903597E-5	0.0	0.0	2.125631798871591	0.0
7	9.417534884903597E-5	0.0	0.0	2.536330495202237	0.0
8	9.417534884903597E-5	0.0	0.0	3.266377799479964	0.0
9	9.417534884903597E-5	0.0	0.0	3.563124323703276	0.0
10	9.417534884903597E-5	0.0	0.0	4.10199566981746	0.0
11	9.417534884903597E-5	0.0	0.0	4.68616535872803	0.0
12	9.417534884903597E-5	0.0	0.0	5.188214143442241	0.0
13	9.417534884903597E-5	0.0	0.0	5.401144607189911	0.0
14	1.8835069769807195E-4	0.0	0.0	5.511047239296736	0.0
15	1.8835069769807195E-4	0.0	0.0	5.677643431410681	0.0
16	1.8835069769807195E-4	0.0	0.0	6.012436796569004	0.0
17	1.8835069769807195E-4	0.0	0.0	6.420404407783027	0.0
18	1.8835069769807195E-4	0.0	0.0	6.883841299469133	0.0
19	1.8835069769807195E-4	0.0	0.0	7.123235036243383	0.0
20	1.8835069769807195E-4	0.0	0.0	7.360651090691802	0.0
21	1.8835069769807195E-4	0.0	0.0	7.647320852588268	0.0
22	1.8835069769807195E-4	0.0	0.0	7.962431569837142	0.0
23	1.8835069769807195E-4	0.0	0.0	8.313140568950953	0.0
24	1.8835069769807195E-4	0.0	0.0	8.596890895033098	0.0
25	1.8835069769807195E-4	0.0	0.0	8.843347782971025	0.0
26	1.8835069769807195E-4	0.0	0.0	9.066920061138637	0.0
27	1.8835069769807195E-4	0.0	0.0	9.317332313728222	0.0
28	1.8835069769807195E-4	0.0	0.0	9.581211641203222	0.0
29	2.825260465471079E-4	0.0	0.0	9.846315248213259	0.0
30	2.825260465471079E-4	0.0	0.0	10.162932771043717	0.0
31	2.825260465471079E-4	0.0	0.0	10.448189902707448	0.0
32	3.767013953961439E-4	0.0	0.0	10.732411105533838	0.0
33	3.767013953961439E-4	0.0	0.0	11.026897421384772	0.0
34	3.767013953961439E-4	0.0	0.0	11.298122426069996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	60	0.0	40.000004	31
CGCGTCA	30	4.8671627E-6	40.000004	39
CATACCG	20	0.0012557255	40.0	1
ACGCATT	140	0.0	38.57143	17
CGTAAGG	190	0.0	35.78947	2
ACGGGAC	405	0.0	35.555557	5
TCTACGA	45	5.4418706E-8	35.555553	22
CGTTTTT	2415	0.0	35.52795	1
TTAACGG	80	0.0	35.0	2
GCAAACG	115	0.0	34.782604	1
TACGCAT	150	0.0	34.666668	16
AAGGGCG	715	0.0	34.405594	5
AACACCG	35	1.4009673E-5	34.285713	1
CATACGA	205	0.0	34.146343	18
GCGGGAT	395	0.0	33.92405	5
TATGCGG	125	0.0	33.6	2
CGAGGGT	275	0.0	33.454544	4
CTTACTG	1300	0.0	33.384617	39
CTTAACG	30	2.2781442E-4	33.333336	1
ACCGCGT	30	2.2781442E-4	33.333336	25
>>END_MODULE