##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553742_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041901
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56140458642424	31.0	31.0	34.0	30.0	34.0
2	31.712916102393606	31.0	31.0	34.0	30.0	34.0
3	31.656603650442797	31.0	31.0	34.0	30.0	34.0
4	35.4708009686141	37.0	35.0	37.0	33.0	37.0
5	33.56913756681297	37.0	35.0	37.0	30.0	37.0
6	34.39524964463994	37.0	35.0	37.0	30.0	37.0
7	35.385780414837875	37.0	35.0	37.0	32.0	37.0
8	35.48320425837004	37.0	35.0	37.0	33.0	37.0
9	37.21596869568222	39.0	37.0	39.0	34.0	39.0
10	36.59766426944594	38.0	35.0	39.0	32.0	39.0
11	36.33419681908358	38.0	35.0	39.0	32.0	39.0
12	36.51940251521018	38.0	35.0	39.0	32.0	39.0
13	32.890251569007035	38.0	35.0	39.0	10.0	39.0
14	35.12171405920524	39.0	34.0	40.0	17.0	41.0
15	36.5199678280374	39.0	34.0	40.0	30.0	41.0
16	37.14502337554144	39.0	36.0	40.0	32.0	41.0
17	37.33344914727983	39.0	36.0	40.0	32.0	41.0
18	37.43456144105822	39.0	36.0	40.0	32.0	41.0
19	37.438040658373495	39.0	36.0	40.0	32.0	41.0
20	37.4357342972125	39.0	36.0	40.0	32.0	41.0
21	37.34564128453663	39.0	36.0	40.0	32.0	41.0
22	37.36898131396361	39.0	36.0	40.0	32.0	41.0
23	37.25266891959985	39.0	35.0	40.0	32.0	41.0
24	37.22393970252452	39.0	35.0	40.0	32.0	41.0
25	37.02875129210933	39.0	35.0	40.0	31.0	41.0
26	36.98319226106895	39.0	35.0	40.0	31.0	41.0
27	36.5883486050978	39.0	35.0	40.0	31.0	41.0
28	36.646596941552026	39.0	35.0	40.0	30.0	41.0
29	36.70500268259652	39.0	35.0	40.0	31.0	41.0
30	36.26257773051374	39.0	35.0	40.0	30.0	41.0
31	36.33775569847807	38.0	35.0	40.0	30.0	41.0
32	36.267911250685046	38.0	35.0	40.0	30.0	41.0
33	36.22466529929427	38.0	35.0	40.0	30.0	41.0
34	36.20420558191229	38.0	35.0	40.0	30.0	41.0
35	36.086604197519726	38.0	35.0	40.0	30.0	41.0
36	35.99985795195513	38.0	35.0	40.0	30.0	41.0
37	35.957867398150114	38.0	35.0	40.0	29.0	41.0
38	35.80081792799892	38.0	35.0	40.0	29.0	41.0
39	35.7603822244148	38.0	35.0	40.0	29.0	41.0
40	35.67005214506945	38.0	34.0	40.0	28.0	41.0
41	35.54271183154638	38.0	34.0	40.0	28.0	41.0
42	35.50082205507049	38.0	34.0	40.0	28.0	41.0
43	35.42663458428392	38.0	34.0	40.0	28.0	41.0
44	35.36382151471205	38.0	34.0	40.0	28.0	41.0
45	35.27999205298776	38.0	34.0	40.0	27.0	41.0
46	34.069989375190154	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	21.0
10	25.0
11	24.0
12	15.0
13	15.0
14	13.0
15	28.0
16	58.0
17	111.0
18	229.0
19	443.0
20	797.0
21	1437.0
22	2238.0
23	3227.0
24	4658.0
25	6673.0
26	9163.0
27	12410.0
28	16392.0
29	20412.0
30	26110.0
31	34284.0
32	43951.0
33	59118.0
34	81717.0
35	101253.0
36	119372.0
37	154723.0
38	189075.0
39	153903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.77777735120707	21.964658830349524	22.02810055849836	22.229463259945042
2	26.288294185340067	27.355766046870094	23.273324432935567	23.08261533485427
3	28.102574044942852	26.98605721656856	23.499737499052216	21.411631239436378
4	24.881346692248112	27.631128101422302	23.153639357290185	24.3338858490394
5	20.642172336911088	34.3044108797285	20.62537611538908	24.42804066797133
6	20.307111712149233	39.23472575609391	21.982030922323716	18.476131609433143
7	81.66735611156915	5.757264845700312	8.477676861813165	4.097702180917381
8	80.9205481134964	5.279964219249238	8.486890789048097	5.3125968782062785
9	73.6000829253451	7.543423031554822	11.892108751215327	6.964385291884738
10	34.440028371217615	26.62306687487583	18.550226940947365	20.3866778129592
11	27.95716675576662	26.168513131285987	24.32774323088278	21.54657688206461
12	24.046622471808742	23.233877306961027	30.996035131936722	21.723465089293512
13	21.251635232138177	30.38273310036174	29.3087347070403	19.056896960459774
14	20.482656221656377	28.16457609696123	29.283972277596433	22.068795403785966
15	19.889893569542597	26.050459688588457	32.413444271576665	21.646202470292284
16	21.53841871732535	24.37515656477919	31.268709791045406	22.817714926850055
17	20.816277170287773	25.148838517287153	28.4717070047922	25.563177307632873
18	22.49455562476665	24.981836086154058	29.0502648524188	23.47334343666049
19	23.38849852337218	27.687179492101453	26.692747199589977	22.23157478493638
20	25.603968131329175	25.15882027179166	26.342809921480065	22.894401675399102
21	25.183006830783345	27.566438653960407	26.742272058477724	20.508282456778524
22	23.432648591372885	24.2250463335768	26.11754859626778	26.224756478782535
23	22.141355080761034	26.67710271897234	26.817999022939798	24.36354317732683
24	22.502233897462425	25.507317873771118	28.957741666434718	23.032706562331736
25	23.388882437006973	26.53850989681361	26.017539094405322	24.055068571774093
26	21.30231183193029	26.44877008468175	26.98097036090761	25.26794772248035
27	22.434185205696128	25.53726313728464	26.889886851053983	25.13866480596525
28	21.711083874571578	26.01293213078786	27.937299225166306	24.33868476947426
29	22.209211815709935	25.92712743341258	25.711847862704808	26.151812888172678
30	22.976559193243887	24.604832896791535	26.658962799728574	25.759645110236
31	24.772987068828996	25.318912257498553	24.1467279520799	25.76137272159255
32	23.59657971342767	25.361718627777496	26.033951402292537	25.007750256502298
33	23.26036734776145	24.30096525485627	26.073110593040987	26.365556804341296
34	23.395504947207076	24.173122014471623	27.82010958814705	24.61126345017425
35	22.447238269278945	23.584102520297034	27.81703827906874	26.151620931355286
36	23.637178580306575	26.696394379120473	25.98116327750909	23.685263763063862
37	22.92809009685181	25.61394988583368	28.47967321271407	22.978286804600437
38	22.723752064735518	27.24404717914658	27.41623244434932	22.615968311768583
39	23.61759898493235	23.500601304730488	28.265833318136753	24.615966392200413
40	23.467584732138658	23.165252744742542	31.02454071931978	22.342621803799016
41	20.26612893163554	25.182622917148556	29.240494058456612	25.31075409275929
42	21.309606190991275	25.982794910456942	27.879904136765393	24.82769476178639
43	22.07004312309903	23.323329183866797	29.91733379658912	24.689293896445054
44	23.766461496821677	23.055165509966876	27.751772961154657	25.42660003205679
45	23.261903002300603	22.809940675745587	27.865891289095607	26.062265032858207
46	23.76185453320421	23.79352740807428	27.33724221399154	25.10737584472997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3785.0
1	3458.5
2	3132.0
3	5701.5
4	8271.0
5	5701.5
6	3132.0
7	3132.0
8	2979.0
9	2826.0
10	2811.5
11	2797.0
12	2834.0
13	2871.0
14	2792.5
15	2714.0
16	2704.5
17	2695.0
18	2600.0
19	2505.0
20	2505.0
21	2718.0
22	2931.0
23	3617.5
24	4304.0
25	5447.5
26	6591.0
27	7515.5
28	8440.0
29	10198.0
30	11956.0
31	11956.0
32	14561.5
33	17167.0
34	20511.5
35	23856.0
36	25735.0
37	27614.0
38	30524.5
39	33435.0
40	38658.0
41	43881.0
42	51039.5
43	58198.0
44	58198.0
45	68060.5
46	77923.0
47	88769.5
48	99616.0
49	103468.0
50	107320.0
51	100644.0
52	93968.0
53	84358.0
54	74748.0
55	69699.5
56	64651.0
57	64651.0
58	60491.0
59	56331.0
60	52663.5
61	48996.0
62	45919.5
63	42843.0
64	37373.5
65	31904.0
66	27356.5
67	22809.0
68	20143.0
69	17477.0
70	17477.0
71	14349.5
72	11222.0
73	9606.5
74	7991.0
75	6378.5
76	4766.0
77	4000.5
78	3235.0
79	2408.0
80	1581.0
81	1581.0
82	1217.0
83	853.0
84	615.5
85	378.0
86	245.0
87	112.0
88	82.0
89	52.0
90	33.0
91	14.0
92	9.5
93	5.0
94	5.0
95	4.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1041901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.945086360247174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16334645862172	27.76916326038958
2	9.679940944514948	7.152525083143948
3	3.7292106476048343	4.133280282939417
4	1.9569079301618928	2.8919252991553472
5	1.2178422448439055	2.2496643454457685
6	0.8634917176206038	1.9141065647310829
7	0.672197101497389	1.738406597615021
8	0.5492286429288993	1.6233039715623632
9	0.48105377175374486	1.5995315827228265
>10	5.536178109793736	40.61319007665816
>50	0.10264902146654314	2.528047059711499
>100	0.04017718068112695	2.7571550866545453
>500	0.005443359957488244	1.3301685988863234
>1k	0.002332868553209247	1.6995321903841312
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4035	0.38727287909311925	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3126	0.30002850558738303	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	2131	0.20452998893368948	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	1672	0.1604758993416841	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT	1649	0.15826839594164896	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGT	1592	0.15279762664590973	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	1531	0.1469429437153818	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.597840869717949E-5	0.0	0.0	0.18782974582038026	0.0
2	9.597840869717949E-5	0.0	0.0	0.7264605754289515	0.0
3	9.597840869717949E-5	0.0	0.0	1.0352231162077778	0.0
4	9.597840869717949E-5	0.0	0.0	1.3920708397438912	0.0
5	9.597840869717949E-5	0.0	0.0	2.460406506952196	0.0
6	9.597840869717949E-5	0.0	0.0	3.86735400004415	0.0
7	9.597840869717949E-5	0.0	0.0	4.746036331666828	0.0
8	9.597840869717949E-5	0.0	0.0	6.226791221046914	0.0
9	9.597840869717949E-5	0.0	0.0	6.90881379324907	0.0
10	9.597840869717949E-5	0.0	0.0	8.05930697830216	0.0
11	9.597840869717949E-5	0.0	0.0	9.396766103497358	0.0
12	9.597840869717949E-5	0.0	0.0	10.461646547992563	0.0
13	9.597840869717949E-5	0.0	0.0	10.980505825409516	0.0
14	9.597840869717949E-5	0.0	0.0	11.200680294960845	0.0
15	9.597840869717949E-5	0.0	0.0	11.533533416322664	0.0
16	9.597840869717949E-5	0.0	0.0	12.23465569185556	0.0
17	9.597840869717949E-5	0.0	0.0	13.017071679554967	0.0
18	9.597840869717949E-5	0.0	0.0	13.957948020013418	0.0
19	2.8793522609153846E-4	0.0	0.0	14.398009023889985	0.0
20	3.8391363478871795E-4	0.0	0.0	14.864848003793067	0.0
21	3.8391363478871795E-4	0.0	0.0	15.42248255832368	0.0
22	3.8391363478871795E-4	0.0	0.0	15.962936977697497	0.0
23	4.7989204348589744E-4	0.0	0.0	16.548885162793777	0.0
24	4.7989204348589744E-4	0.0	0.0	17.02925709832316	0.0
25	4.7989204348589744E-4	0.0	0.0	17.44781893865156	0.0
26	4.7989204348589744E-4	0.0	0.0	17.828757242770667	0.0
27	4.7989204348589744E-4	0.0	0.0	18.231386667255336	0.0
28	4.7989204348589744E-4	0.0	0.0	18.657434823462115	0.0
29	4.7989204348589744E-4	0.0	0.0	19.095768215982133	0.0
30	4.7989204348589744E-4	0.0	0.0	19.643229059190844	0.0
31	4.7989204348589744E-4	0.0	0.0	20.09605519142414	0.0
32	4.7989204348589744E-4	0.0	0.0	20.57105233606648	0.0
33	4.7989204348589744E-4	0.0	0.0	21.03002108645639	0.0
34	4.7989204348589744E-4	0.0	0.0	21.453957717671834	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAC	20	0.0012557148	40.0	34
ATAGCGC	25	7.794153E-5	40.0	12
ACGATAA	25	7.794153E-5	40.0	34
CATAACG	35	3.0560113E-7	40.0	1
GCGTGAT	20	0.0012557148	40.0	32
AATAGCG	65	0.0	40.0	1
TCGAAAG	30	4.867077E-6	40.0	1
GCCGAAC	90	0.0	37.77778	4
TACGCAT	75	0.0	37.333332	16
CTATGCG	50	3.423338E-9	36.0	1
GTATACG	50	3.423338E-9	36.0	1
CGGTCTA	50	3.423338E-9	36.0	31
TATGCGG	150	0.0	35.999996	2
CGTAAGG	150	0.0	35.999996	2
ACTTACT	1260	0.0	35.714283	38
CTATACG	45	5.4416887E-8	35.555557	1
GTCAACG	45	5.4416887E-8	35.555557	1
CAAATCG	45	5.4416887E-8	35.555557	1
TGTACCG	40	8.69888E-7	35.0	1
GCACTTA	1310	0.0	34.809162	36
>>END_MODULE