FastQCFastQC Report
Sat 18 Jun 2016
SRR3553740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865819
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159211.8388369855593376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19480.22498928759937123No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.19299645768919368No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTC15230.17590281571552485No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC13360.15430476808663243No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC12670.14633543500431384No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT11770.13594065272302872No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG9800.11318762928510463No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT9070.10475630587917337No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTT8800.10163787119478783No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.10117588087117516No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG8660.10002090506214348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC200.001255597140.08
TAGACCG200.001255597140.01
TAACGGC200.001255597140.03
CTCGACG304.8661204E-640.01
GCTACGA304.8661204E-640.010
GCCGTTG200.001255597140.01
AATCGCG200.001255597140.01
CAGACGA304.8661204E-640.028
TTGCGAG353.055211E-739.9999961
CGTTTTT91000.038.9450531
CGTTATT4750.037.8947371
GCGAGAC1800.037.77777521
TAGCGAG650.036.9230771
ATAACGG650.036.9230772
ACACGAC1800.036.66666426
ACGGGTA1700.036.470595
CCCTCGT552.1645974E-1036.36363614
CTATACG503.423338E-936.01
CGCACGG1500.036.02
CGCATGG1850.035.6756742
Produced by FastQC (version 0.11.5)