##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553740_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 865819 Sequences flagged as poor quality 0 Sequence length 46 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.723069140316856 31.0 31.0 34.0 30.0 34.0 2 31.912661884296835 31.0 31.0 34.0 30.0 34.0 3 31.565173552439944 31.0 31.0 34.0 30.0 34.0 4 35.5150649269651 37.0 35.0 37.0 33.0 37.0 5 33.693647286557585 37.0 35.0 37.0 30.0 37.0 6 34.56726405865429 37.0 35.0 37.0 30.0 37.0 7 35.58561084938076 37.0 35.0 37.0 33.0 37.0 8 35.70976035406938 37.0 35.0 37.0 35.0 37.0 9 37.51002807746192 39.0 37.0 39.0 35.0 39.0 10 36.907032532203615 39.0 37.0 39.0 33.0 39.0 11 36.65685206723345 39.0 37.0 39.0 32.0 39.0 12 36.90263438432282 39.0 37.0 39.0 33.0 39.0 13 33.24176300127394 39.0 35.0 39.0 10.0 39.0 14 35.57230552806072 39.0 35.0 41.0 17.0 41.0 15 37.01463816340367 39.0 35.0 41.0 30.0 41.0 16 37.636631905744736 39.0 36.0 41.0 32.0 41.0 17 37.82429468514782 39.0 37.0 41.0 33.0 41.0 18 37.81995428605748 39.0 37.0 40.0 33.0 41.0 19 37.69056234617166 39.0 37.0 40.0 33.0 41.0 20 37.5589274432647 39.0 35.0 41.0 33.0 41.0 21 37.475222881456745 39.0 35.0 40.0 33.0 41.0 22 37.48212386191571 39.0 35.0 41.0 33.0 41.0 23 37.358558774986456 39.0 35.0 40.0 33.0 41.0 24 37.29557909909577 39.0 35.0 40.0 33.0 41.0 25 37.11272910388892 39.0 35.0 40.0 32.0 41.0 26 37.08394941667947 39.0 35.0 40.0 32.0 41.0 27 36.68252833444404 39.0 35.0 40.0 32.0 41.0 28 36.710499538587165 39.0 35.0 40.0 31.0 41.0 29 36.747299377814535 39.0 35.0 40.0 31.0 41.0 30 36.21312999599223 38.0 35.0 40.0 30.0 41.0 31 36.15574040301726 38.0 35.0 40.0 30.0 41.0 32 35.871824249641094 38.0 35.0 40.0 29.0 41.0 33 35.53231333569718 38.0 35.0 40.0 26.0 41.0 34 35.22681761430507 38.0 35.0 40.0 24.0 41.0 35 35.029645919066226 38.0 35.0 40.0 23.0 41.0 36 34.81106097232794 38.0 34.0 40.0 22.0 41.0 37 34.724534804618514 38.0 34.0 40.0 21.0 41.0 38 34.58513499934744 38.0 34.0 40.0 21.0 41.0 39 34.49172979571943 38.0 34.0 40.0 20.0 41.0 40 34.420189439132194 38.0 34.0 40.0 20.0 41.0 41 34.26914516775446 38.0 34.0 40.0 18.0 41.0 42 34.16831924455342 38.0 33.0 40.0 18.0 41.0 43 34.05209056396314 38.0 33.0 40.0 18.0 41.0 44 33.96373375959641 38.0 33.0 40.0 18.0 41.0 45 33.90078526805256 38.0 33.0 40.0 18.0 41.0 46 32.83473220153404 36.0 31.0 40.0 15.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 11.0 10 20.0 11 12.0 12 9.0 13 3.0 14 17.0 15 25.0 16 36.0 17 100.0 18 187.0 19 375.0 20 800.0 21 1375.0 22 2314.0 23 3535.0 24 5603.0 25 8565.0 26 13117.0 27 18872.0 28 22259.0 29 22524.0 30 22861.0 31 26001.0 32 31913.0 33 41566.0 34 58174.0 35 75779.0 36 90982.0 37 122863.0 38 155736.0 39 140182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.537098400474 20.82051791425229 20.184241741056734 28.45814194421698 2 34.000639856598205 23.75808338694346 21.00658451708729 21.234692239371046 3 25.36061232197492 24.024420808506168 30.69787103309121 19.917095836427706 4 22.193091165705532 25.397687045444833 30.174666991599864 22.23455479724977 5 19.041046685277177 32.43345318132312 27.53208234053538 20.993417792864328 6 17.84864966003287 36.95864840110924 28.743189973885997 16.449511964971894 7 76.71222276249424 4.228481934445883 15.62867065749308 3.430624645566799 8 76.77759439328543 3.8478019077890413 15.374344984344305 4.000258714581223 9 69.95965669499053 6.3070918979601975 18.327387132876503 5.405864274172777 10 30.114723747111118 25.748337701066852 26.373641604076603 17.76329694774543 11 24.57291882021531 25.24523023865265 32.35225838194819 17.829592559183848 12 20.454506080370148 22.916337017321172 37.02147908512056 19.60767781718812 13 18.86294941552449 29.45130564240332 35.58214823190528 16.103596710166908 14 19.444479735371942 26.130865689018144 35.67108136920073 18.75357320640919 15 18.785335041157563 25.224556171670987 38.055875419689336 17.93423336748212 16 20.18562771202757 24.2407477775378 36.78263008781281 18.790994422621818 17 20.112517743315866 24.595440848491428 34.69616628879708 20.59587511939562 18 20.864637990157295 24.131371568422498 35.31084441436374 19.693146027056464 19 21.61179184101989 26.547234468174064 33.46969747718634 18.371276213619705 20 23.368163553814366 24.009868113312365 33.44128507228416 19.180683260589106 21 22.93885904559729 25.456128821381835 33.82161860619829 17.78339352682258 22 21.922711328811218 23.21478276637496 33.30326546310488 21.559240441708948 23 20.123259018339862 25.983144283042993 33.449138907785574 20.44445779083157 24 20.098196043283874 24.240054792052383 35.23542449403397 20.42632467062977 25 21.240813611158917 25.605236198327823 32.93205623808209 20.221893952431166 26 19.898154233159588 25.6114730676966 34.34262819365249 20.147744505491332 27 21.27118947493645 24.895388066096956 33.57676373468358 20.25665872428302 28 20.430482583542286 25.330005463035576 34.740401862282994 19.49911009113914 29 21.083274910806992 24.5651804822948 33.32578749138099 21.025757115517216 30 21.734565769519957 24.597057814624073 33.854650914336595 19.81372550151937 31 22.449033804986954 24.671322759144807 32.207770908238324 20.67187252762991 32 21.882864663399626 25.273758141135733 32.83319030882898 20.01018688663566 33 22.759606800035574 25.252737581411356 30.952543198982696 21.035112419570375 34 20.76484808025696 25.870534141662404 32.587988944571556 20.77662883350908 35 23.03807146759311 24.506623208776894 30.814408092222507 21.64089723140749 36 23.212126322014186 27.014306685346472 29.51321234576742 20.26035464687192 37 22.69712260876696 27.563382184960133 29.143042598972762 20.59645260730014 38 22.468668393740494 27.08522220002102 30.22640990784448 20.219699498394007 39 22.37996625160686 25.816827766542428 29.459159477904734 22.344046503945975 40 22.946019895613286 24.82805297643041 31.52679717123325 20.699129956723056 41 21.28724363868199 25.872151107795048 30.499099696356858 22.341505557166105 42 22.225314990777516 27.044105061219494 29.56102834426133 21.16955160374166 43 22.01626436934278 25.717961837289316 30.653519962024394 21.6122538313435 44 22.70301298539302 26.234582516669185 29.45384658918319 21.608557908754598 45 22.40295027020659 24.895734558839667 30.170162585944638 22.531152585009107 46 22.883535704344673 25.20619205630738 29.36884036963846 22.541431869709488 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2278.0 1 2009.0 2 1740.0 3 15564.5 4 29389.0 5 20012.5 6 10636.0 7 10636.0 8 9870.0 9 9104.0 10 8864.0 11 8624.0 12 8409.0 13 8194.0 14 7901.0 15 7608.0 16 7214.5 17 6821.0 18 6466.5 19 6112.0 20 6112.0 21 5898.5 22 5685.0 23 5972.0 24 6259.0 25 6903.0 26 7547.0 27 8599.0 28 9651.0 29 11268.0 30 12885.0 31 12885.0 32 14579.0 33 16273.0 34 18487.5 35 20702.0 36 23346.0 37 25990.0 38 29514.5 39 33039.0 40 37065.0 41 41091.0 42 45839.0 43 50587.0 44 50587.0 45 57797.5 46 65008.0 47 72271.5 48 79535.0 49 81626.5 50 83718.0 51 77144.5 52 70571.0 53 62039.5 54 53508.0 55 48972.5 56 44437.0 57 44437.0 58 40731.0 59 37025.0 60 34289.0 61 31553.0 62 28262.5 63 24972.0 64 21468.0 65 17964.0 66 15320.0 67 12676.0 68 10961.5 69 9247.0 70 9247.0 71 7496.0 72 5745.0 73 4717.5 74 3690.0 75 3286.0 76 2882.0 77 2191.5 78 1501.0 79 1164.0 80 827.0 81 827.0 82 652.5 83 478.0 84 309.0 85 140.0 86 100.5 87 61.0 88 50.0 89 39.0 90 23.5 91 8.0 92 11.5 93 15.0 94 15.0 95 8.0 96 1.0 97 1.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 865819.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.53687130914087 #Duplication Level Percentage of deduplicated Percentage of total 1 75.68967991171841 34.466712135699865 2 9.120872667628293 8.306720097857 3 3.8817186016477163 5.302839612645909 4 2.3230272257294313 4.231335673026867 5 1.5996461463895206 3.642144035415136 6 1.2447800353463603 3.401003296665302 7 1.024213791264839 3.2647644124111967 8 0.8187539986065615 2.98267963747132 9 0.7058466611692273 2.8927843716284882 >10 3.5107550204361715 24.119034483524192 >50 0.05464762908559187 1.6709638096094412 >100 0.02150443139062718 1.9060706660031943 >500 0.0027829264144574087 0.9509040359373888 >1k 0.001517959862431314 1.0278611350416018 >5k 0.0 0.0 >10k+ 2.5299331040521897E-4 1.8341825970631411 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15921 1.8388369855593376 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1948 0.22498928759937123 No Hit CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1671 0.19299645768919368 No Hit CGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTC 1523 0.17590281571552485 No Hit CGTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC 1336 0.15430476808663243 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC 1267 0.14633543500431384 No Hit CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT 1177 0.13594065272302872 No Hit GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG 980 0.11318762928510463 No Hit GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT 907 0.10475630587917337 No Hit CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTT 880 0.10163787119478783 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.10117588087117516 No Hit CGTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG 866 0.10002090506214348 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.12069497204381054 0.0 2 0.0 0.0 0.0 0.47608102848285844 0.0 3 0.0 0.0 0.0 0.8157594139190755 0.0 4 0.0 0.0 0.0 1.136496196087173 0.0 5 0.0 0.0 0.0 1.8572011009229412 0.0 6 0.0 0.0 0.0 3.039780831790478 0.0 7 0.0 0.0 0.0 3.84664693198001 0.0 8 0.0 0.0 0.0 5.1785650349553425 0.0 9 0.0 0.0 0.0 5.829740396087404 0.0 10 0.0 0.0 0.0 6.881114875048942 0.0 11 0.0 0.0 0.0 7.841477260258784 0.0 12 0.0 0.0 0.0 8.640027534623288 0.0 13 0.0 0.0 0.0 9.05316238151392 0.0 14 0.0 0.0 0.0 9.264176461824007 0.0 15 0.0 0.0 0.0 9.50418043494079 0.0 16 0.0 0.0 0.0 9.991811221513965 0.0 17 0.0 0.0 0.0 10.537537291281435 0.0 18 0.0 0.0 0.0 11.22313093152264 0.0 19 0.0 0.0 0.0 11.55634145242828 0.0 20 0.0 0.0 0.0 11.880658659604375 0.0 21 0.0 0.0 0.0 12.264688116107408 0.0 22 0.0 0.0 0.0 12.658765862149018 0.0 23 0.0 0.0 0.0 13.121795663989818 0.0 24 0.0 0.0 0.0 13.482725604312218 0.0 25 0.0 0.0 0.0 13.812471197790762 0.0 26 0.0 1.1549758090316798E-4 0.0 14.107336521836551 0.0 27 0.0 1.1549758090316798E-4 0.0 14.453020781479731 0.0 28 0.0 1.1549758090316798E-4 0.0 14.797319070152076 0.0 29 0.0 1.1549758090316798E-4 0.0 15.146930247545965 0.0 30 0.0 1.1549758090316798E-4 0.0 15.59991175984819 0.0 31 0.0 1.1549758090316798E-4 0.0 15.947328483204918 0.0 32 0.0 1.1549758090316798E-4 0.0 16.309182404174546 0.0 33 0.0 1.1549758090316798E-4 0.0 16.663182489642754 0.0 34 0.0 1.1549758090316798E-4 0.0 16.99327457586401 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACGC 20 0.0012555971 40.0 8 TAGACCG 20 0.0012555971 40.0 1 TAACGGC 20 0.0012555971 40.0 3 CTCGACG 30 4.8661204E-6 40.0 1 GCTACGA 30 4.8661204E-6 40.0 10 GCCGTTG 20 0.0012555971 40.0 1 AATCGCG 20 0.0012555971 40.0 1 CAGACGA 30 4.8661204E-6 40.0 28 TTGCGAG 35 3.055211E-7 39.999996 1 CGTTTTT 9100 0.0 38.945053 1 CGTTATT 475 0.0 37.894737 1 GCGAGAC 180 0.0 37.777775 21 TAGCGAG 65 0.0 36.923077 1 ATAACGG 65 0.0 36.923077 2 ACACGAC 180 0.0 36.666664 26 ACGGGTA 170 0.0 36.47059 5 CCCTCGT 55 2.1645974E-10 36.363636 14 CTATACG 50 3.423338E-9 36.0 1 CGCACGG 150 0.0 36.0 2 CGCATGG 185 0.0 35.675674 2 >>END_MODULE