FastQCFastQC Report
Sat 18 Jun 2016
SRR3553738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668788
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.5192975950525428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23460.35078380592953223No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT13870.20739008475032447No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT10740.1605890057835966No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG9530.14249657589550052No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTT8770.13113273563520877No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC7190.10750790983091803No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG200.001255392140.0000041
CACTCGA200.001255392140.00000417
TATTCCG200.001255392140.00000425
ACCACGA200.001255392140.00000422
TCACGAG257.791155E-540.01
GCGATAT451.2205419E-940.09
GTATACG353.0538286E-740.01
GCGTAAG257.791155E-540.01
ACGCATT1050.038.0952417
TACGCAT1050.038.0952416
CTAACGG700.037.1428572
AGTACGG650.036.9230772
ACGGGAT3700.036.7567565
CGTTTTT15600.036.5384641
AAGCGTA503.4197E-936.022
CGACTAA503.4197E-936.017
TAAACGG950.035.7894742
TCGATCA950.035.78947417
TTACGGG1850.035.6756743
TAAGCGA455.4367774E-835.55555313