##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553738_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	668788
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624987290441815	31.0	31.0	34.0	30.0	34.0
2	31.78950130684163	31.0	31.0	34.0	30.0	34.0
3	31.701877126981945	31.0	31.0	34.0	30.0	34.0
4	35.50556379600113	37.0	35.0	37.0	33.0	37.0
5	33.61656160098566	37.0	35.0	37.0	30.0	37.0
6	34.449815188071554	37.0	35.0	37.0	30.0	37.0
7	35.47477676034857	37.0	35.0	37.0	32.0	37.0
8	35.59519758129632	37.0	35.0	37.0	35.0	37.0
9	37.34380401562229	39.0	37.0	39.0	35.0	39.0
10	36.664633635770976	39.0	37.0	39.0	32.0	39.0
11	36.411368026938284	39.0	35.0	39.0	32.0	39.0
12	36.57700048445845	39.0	35.0	39.0	32.0	39.0
13	32.96186833495816	38.0	35.0	39.0	10.0	39.0
14	35.13925638617918	39.0	34.0	40.0	17.0	41.0
15	36.54878227480158	39.0	35.0	40.0	30.0	41.0
16	37.198892623671476	39.0	36.0	41.0	32.0	41.0
17	37.398073829075884	39.0	36.0	40.0	32.0	41.0
18	37.49549034970723	39.0	36.0	40.0	32.0	41.0
19	37.47891708583288	39.0	36.0	40.0	32.0	41.0
20	37.46026842586888	39.0	36.0	41.0	32.0	41.0
21	37.37885996758315	39.0	36.0	40.0	32.0	41.0
22	37.42118279634204	39.0	36.0	41.0	32.0	41.0
23	37.29403488100863	39.0	35.0	40.0	32.0	41.0
24	37.27592151773058	39.0	35.0	40.0	32.0	41.0
25	37.08939753703715	39.0	35.0	40.0	32.0	41.0
26	37.05587869399571	39.0	35.0	40.0	32.0	41.0
27	36.633247008020476	39.0	35.0	40.0	31.0	41.0
28	36.72076801617254	39.0	35.0	40.0	31.0	41.0
29	36.77132962912014	39.0	35.0	40.0	31.0	41.0
30	36.254336202204584	39.0	35.0	40.0	30.0	41.0
31	36.3168074187934	38.0	35.0	40.0	30.0	41.0
32	36.27963121347871	38.0	35.0	40.0	30.0	41.0
33	36.168711161085426	38.0	35.0	40.0	30.0	41.0
34	36.14305579645568	38.0	35.0	40.0	30.0	41.0
35	36.06641117962643	38.0	35.0	40.0	30.0	41.0
36	35.93537413948815	38.0	35.0	40.0	29.0	41.0
37	35.927848585799985	38.0	35.0	40.0	29.0	41.0
38	35.82768081963193	38.0	35.0	40.0	29.0	41.0
39	35.768920794033384	38.0	35.0	40.0	29.0	41.0
40	35.68078075563557	38.0	35.0	40.0	28.0	41.0
41	35.51211445181433	38.0	34.0	40.0	27.0	41.0
42	35.4515840595226	38.0	34.0	40.0	27.0	41.0
43	35.3440881116288	38.0	34.0	40.0	27.0	41.0
44	35.27520230626148	38.0	34.0	40.0	27.0	41.0
45	35.21984993749888	38.0	34.0	40.0	27.0	41.0
46	34.018847527168546	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	7.0
10	12.0
11	12.0
12	7.0
13	10.0
14	18.0
15	14.0
16	42.0
17	80.0
18	158.0
19	323.0
20	540.0
21	988.0
22	1534.0
23	2207.0
24	3187.0
25	4486.0
26	6141.0
27	8287.0
28	10977.0
29	13161.0
30	16649.0
31	21336.0
32	27440.0
33	37092.0
34	51178.0
35	62409.0
36	74310.0
37	98481.0
38	121685.0
39	106009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.05845798668636	23.32024498047214	22.14857323995048	21.47272379289102
2	26.892827024408334	26.377865631560375	23.28839632290053	23.440911021130763
3	27.768291297092652	27.31882150995532	23.566361836635824	21.3465253563162
4	24.300675251350203	28.21791060844393	23.304096365365407	24.177317774840457
5	20.46463154243198	34.33389953169016	21.05300932432998	24.148459601547874
6	19.879244244813005	39.62929956877217	22.280304072441492	18.211152113973338
7	83.15549920154069	4.751879519369367	8.161480170098747	3.93114110899119
8	82.7586619377142	4.452532043039049	8.0874656841929	4.7013403350538585
9	75.6873628115337	7.064570536552689	11.173196887503963	6.074869764409648
10	33.48998486814955	30.42877563592648	17.682883066083722	18.39835642984025
11	27.206229776850062	26.719229412010982	27.002278749020615	19.07226206211834
12	23.480983510469684	24.649365718284418	30.889758787538053	20.97989198370784
13	21.10728661399427	30.515798728446086	30.36567641763907	18.011238239920573
14	20.683385467442598	28.033547252642094	30.21271314676699	21.07035413314832
15	19.780408739391255	26.534267959353336	33.01375024671495	20.671573054540453
16	22.386316740132898	26.018857993863527	31.04257253419619	20.55225273180739
17	22.142891319820333	25.922115827437096	28.76666447364486	23.168328379097712
18	23.794236738697464	25.01151336447424	29.225853334689017	21.968396562139272
19	24.067716526014223	27.437095163190726	27.59260034569998	20.90258796509507
20	25.820738410378176	25.746424876044426	26.79668295483771	21.63615375873969
21	24.853316746113865	27.318522461527422	28.029958671507266	19.798202120851453
22	23.910716101365455	24.184494937110117	28.12954179799877	23.775247163525663
23	21.643779493651202	27.65779290298271	27.74451694707441	22.95391065629168
24	21.728410198747586	26.160457424475318	29.531480828005286	22.57965154877181
25	22.93282774212456	27.242265112412305	27.00317589430432	22.821731251158813
26	22.194327649419545	27.076592283354366	27.632971883466805	23.096108183759277
27	22.21556008780062	27.172736352924993	27.538323056035697	23.073380503238695
28	21.784481778979288	27.336913939843416	28.00184811928444	22.876756161892857
29	23.806348200027514	26.51617552946524	26.6348977553425	23.042578515164745
30	24.797843262737967	26.88624795899448	26.9134613659336	21.402447412333952
31	25.04590393368302	27.221182198245188	25.52393284568503	22.208981022386766
32	26.34242839285394	26.79593533376795	25.43661070473753	21.425025568640585
33	24.646375234005394	27.59050700670467	24.66446766389349	23.098650095396447
34	23.620938174728014	26.79339342213078	27.66930626745695	21.916362135684253
35	23.681794529806158	26.901499428817505	26.89850894453848	22.51819709683786
36	24.351812532521517	29.704629867760783	24.574454087094864	21.369103512622832
37	23.915500876211894	28.442346453584694	26.22355664276273	21.418596027440685
38	23.256846713756826	28.517258084774248	26.04771616715611	22.178179034312816
39	23.215278982278388	26.76632953940561	26.326279777747207	23.69211170056879
40	24.12199381567851	25.430181163537625	28.423207354198937	22.024617666584927
41	20.86700120217468	26.971028188304814	27.648522401717734	24.51344820780277
42	23.48038541361388	26.74569519788034	26.812233473088632	22.961685915417142
43	22.8324969945633	25.478477484643864	28.399433004180697	23.28959251661214
44	24.54051209052794	25.92615298121378	26.33241027051921	23.200924657739076
45	23.54079319605017	24.317122914884838	27.442926607534822	24.69915728153017
46	23.49025401173466	25.582247289126002	26.90463943731048	24.02285926182886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2730.0
1	2347.0
2	1964.0
3	4441.0
4	6918.0
5	4753.5
6	2589.0
7	2589.0
8	2420.0
9	2251.0
10	2306.0
11	2361.0
12	2228.0
13	2095.0
14	2052.5
15	2010.0
16	1974.5
17	1939.0
18	1913.0
19	1887.0
20	1887.0
21	2086.5
22	2286.0
23	2563.0
24	2840.0
25	3802.0
26	4764.0
27	5499.0
28	6234.0
29	7691.0
30	9148.0
31	9148.0
32	10629.5
33	12111.0
34	14192.0
35	16273.0
36	18226.0
37	20179.0
38	22363.5
39	24548.0
40	27845.5
41	31143.0
42	35699.0
43	40255.0
44	40255.0
45	46607.5
46	52960.0
47	58943.0
48	64926.0
49	66090.0
50	67254.0
51	62775.5
52	58297.0
53	52614.5
54	46932.0
55	43072.5
56	39213.0
57	39213.0
58	36471.0
59	33729.0
60	31271.5
61	28814.0
62	26543.0
63	24272.0
64	20749.5
65	17227.0
66	14731.0
67	12235.0
68	10414.5
69	8594.0
70	8594.0
71	7566.5
72	6539.0
73	5523.5
74	4508.0
75	3755.0
76	3002.0
77	2556.0
78	2110.0
79	1555.5
80	1001.0
81	1001.0
82	712.0
83	423.0
84	294.0
85	165.0
86	103.0
87	41.0
88	26.5
89	12.0
90	8.5
91	5.0
92	3.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	668788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.80638724161313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.34854723511553	32.68205478280947
2	8.57772753876495	7.343630533548431
3	3.4643698642938827	4.448914738774159
4	2.00283707739205	3.429368780668191
5	1.3354346291989694	2.8582565936675564
6	1.0501167455996478	2.6971022436624645
7	0.8840964315228559	2.649148194468697
8	0.7691463317303213	2.6339500577211497
9	0.6516461895819748	2.5105157222190897
>10	4.8418464938645736	33.5891322126561
>50	0.046134508504102584	1.3679930722939677
>100	0.024975070605522082	2.025187944984317
>500	0.001734379903161224	0.5352886972408819
>1k	0.0013875039225289792	1.2294564252855764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3473	0.5192975950525428	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2346	0.35078380592953223	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT	1387	0.20739008475032447	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT	1074	0.1605890057835966	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	953	0.14249657589550052	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTT	877	0.13113273563520877	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC	719	0.10750790983091803	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15131850451862175	0.0
2	0.0	0.0	0.0	0.6888580536732118	0.0
3	0.0	0.0	0.0	1.009886541026454	0.0
4	0.0	0.0	0.0	1.3513998456910112	0.0
5	0.0	0.0	0.0	2.291159530374349	0.0
6	0.0	0.0	0.0	3.5799087304198043	0.0
7	0.0	0.0	0.0	4.371041346435642	0.0
8	0.0	0.0	0.0	5.812006196283426	0.0
9	0.0	0.0	0.0	6.452717453064349	0.0
10	0.0	0.0	0.0	7.556654724666112	0.0
11	0.0	0.0	0.0	8.720401681848358	0.0
12	0.0	0.0	0.0	9.681842377554622	0.0
13	0.0	0.0	0.0	10.123536905566487	0.0
14	0.0	0.0	0.0	10.327188884968031	0.0
15	0.0	0.0	0.0	10.63042399086108	0.0
16	0.0	0.0	0.0	11.283396233186002	0.0
17	0.0	0.0	0.0	12.040287804207013	0.0
18	0.0	0.0	0.0	12.926069247653965	0.0
19	0.0	0.0	0.0	13.347129434140564	0.0
20	0.0	0.0	0.0	13.77626392818053	0.0
21	0.0	0.0	0.0	14.274927181707806	0.0
22	0.0	0.0	0.0	14.789888574555764	0.0
23	0.0	0.0	0.0	15.346118650454255	0.0
24	0.0	0.0	0.0	15.799027494512462	0.0
25	0.0	0.0	0.0	16.189584741353016	0.0
26	0.0	0.0	0.0	16.5778991249843	0.0
27	0.0	0.0	0.0	16.994623109266314	0.0
28	0.0	0.0	0.0	17.38413368660921	0.0
29	0.0	0.0	0.0	17.799661477179615	0.0
30	0.0	0.0	0.0	18.333313396771473	0.0
31	0.0	0.0	0.0	18.775456497425193	0.0
32	0.0	0.0	0.0	19.20010526504662	0.0
33	0.0	0.0	0.0	19.611894950268248	0.0
34	0.0	0.0	0.0	20.040580871666357	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGCG	20	0.0012553921	40.000004	1
CACTCGA	20	0.0012553921	40.000004	17
TATTCCG	20	0.0012553921	40.000004	25
ACCACGA	20	0.0012553921	40.000004	22
TCACGAG	25	7.791155E-5	40.0	1
GCGATAT	45	1.2205419E-9	40.0	9
GTATACG	35	3.0538286E-7	40.0	1
GCGTAAG	25	7.791155E-5	40.0	1
ACGCATT	105	0.0	38.09524	17
TACGCAT	105	0.0	38.09524	16
CTAACGG	70	0.0	37.142857	2
AGTACGG	65	0.0	36.923077	2
ACGGGAT	370	0.0	36.756756	5
CGTTTTT	1560	0.0	36.538464	1
AAGCGTA	50	3.4197E-9	36.0	22
CGACTAA	50	3.4197E-9	36.0	17
TAAACGG	95	0.0	35.789474	2
TCGATCA	95	0.0	35.789474	17
TTACGGG	185	0.0	35.675674	3
TAAGCGA	45	5.4367774E-8	35.555553	13
>>END_MODULE