FastQCFastQC Report
Sat 18 Jun 2016
SRR3553737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466038
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29720.6377162377316871No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG26650.5718417811423103No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTA22330.4791454774074217No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCG21770.4671292898862324No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15920.3416030452452375No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT14400.30898767911629527No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT11940.25620228393392813No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTT10980.23560310532617512No Hit
TGATGAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACC9200.19740879499096642No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.155352138666804No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6570.1409756285968097No Hit
GAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGT5750.12338049686935401No Hit
TCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT5410.1160849544457748No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC5380.11544123011428253No Hit
TGATGAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGT5310.11393920667413387No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA5270.11308090756547749No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGT4820.10342504259309326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCACG304.8612765E-640.0000041
ACCGTAG304.8612765E-640.00000426
TAGTACG304.8612765E-640.0000041
GTACGAG304.8612765E-640.0000041
TCGTTTA200.001255000640.038
CTCGTCA200.001255000640.036
CACGACT200.001255000640.012
TCTAGCG200.001255000640.01
CGTTAGG451.2187229E-940.02
CTACGCG257.787513E-540.01
TTGACGG200.001255000640.02
ACTACGG401.924127E-840.02
CTCATAA200.001255000640.018
ACAGACG200.001255000640.01
AATAGCG257.787513E-540.01
ATCGCCA200.001255000640.024
TGCACCA257.787513E-540.028
GCATCGT257.787513E-540.035
ACGGGTA650.039.9999965
TTAACGG900.037.777782
Produced by FastQC (version 0.11.5)