##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466038
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.540949450473995	31.0	31.0	34.0	30.0	34.0
2	31.700545878233104	31.0	31.0	34.0	30.0	34.0
3	31.649507121736853	31.0	31.0	34.0	30.0	34.0
4	35.45352524901403	37.0	35.0	37.0	33.0	37.0
5	33.57046850256846	37.0	35.0	37.0	30.0	37.0
6	34.3920581583476	37.0	35.0	37.0	30.0	37.0
7	35.29800788776881	37.0	35.0	37.0	32.0	37.0
8	35.34228539303662	37.0	35.0	37.0	33.0	37.0
9	37.14426291418297	39.0	37.0	39.0	34.0	39.0
10	36.54988863569065	38.0	35.0	39.0	32.0	39.0
11	36.27777992352555	38.0	35.0	39.0	32.0	39.0
12	36.440719426312874	38.0	35.0	39.0	32.0	39.0
13	32.7839425111257	37.0	34.0	39.0	10.0	39.0
14	35.00248048442402	38.0	34.0	40.0	17.0	41.0
15	36.394663096142374	39.0	34.0	40.0	29.0	41.0
16	37.06992133688669	39.0	36.0	40.0	32.0	41.0
17	37.222196473248964	39.0	36.0	40.0	32.0	41.0
18	37.32859337650578	39.0	36.0	40.0	32.0	41.0
19	37.294853638544495	39.0	36.0	40.0	32.0	41.0
20	37.302619099730066	39.0	35.0	40.0	32.0	41.0
21	37.23152189306452	39.0	35.0	40.0	32.0	41.0
22	37.24078723194246	39.0	35.0	40.0	32.0	41.0
23	37.12018762418515	39.0	35.0	40.0	32.0	41.0
24	37.080753071637936	39.0	35.0	40.0	32.0	41.0
25	36.89780017938451	39.0	35.0	40.0	31.0	41.0
26	36.85333813980834	39.0	35.0	40.0	31.0	41.0
27	36.38374982297581	39.0	35.0	40.0	30.0	41.0
28	36.47393560181788	39.0	35.0	40.0	30.0	41.0
29	36.549897218681735	39.0	35.0	40.0	30.0	41.0
30	36.03673091035495	38.0	35.0	40.0	30.0	41.0
31	36.134338830739125	38.0	35.0	40.0	30.0	41.0
32	36.06889352370408	38.0	35.0	40.0	30.0	41.0
33	35.984758753578035	38.0	35.0	40.0	30.0	41.0
34	35.96213613482163	38.0	35.0	40.0	30.0	41.0
35	35.867712504130566	38.0	35.0	40.0	29.0	41.0
36	35.734455130268344	38.0	35.0	40.0	29.0	41.0
37	35.65739918204095	38.0	35.0	40.0	28.0	41.0
38	35.53706135551178	38.0	34.0	40.0	28.0	41.0
39	35.49024757637789	38.0	34.0	40.0	28.0	41.0
40	35.315371278736926	38.0	34.0	40.0	27.0	41.0
41	35.13476368879791	38.0	34.0	40.0	26.0	41.0
42	35.08169505490969	38.0	34.0	40.0	26.0	41.0
43	34.975040661920275	38.0	34.0	40.0	26.0	41.0
44	34.89611791313155	37.0	34.0	40.0	26.0	41.0
45	34.87261982928431	37.0	34.0	40.0	26.0	41.0
46	33.65612889936014	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	9.0
11	13.0
12	10.0
13	8.0
14	10.0
15	10.0
16	31.0
17	54.0
18	150.0
19	269.0
20	485.0
21	844.0
22	1290.0
23	1813.0
24	2616.0
25	3549.0
26	4783.0
27	6134.0
28	7793.0
29	9803.0
30	12289.0
31	15895.0
32	20245.0
33	27262.0
34	37449.0
35	47067.0
36	51745.0
37	67620.0
38	81272.0
39	65515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.802526832575886	22.04648547972483	22.174372046914627	20.976615640784658
2	25.688892322085323	28.732206386603664	23.159055699320657	22.419845591990352
3	26.89609001841052	30.170501117934588	22.50610465241032	20.427304211244575
4	24.028512696389566	27.3587132379763	25.026070835425436	23.586703230208695
5	21.72483788875585	34.58065651298821	19.72156776915187	23.972937829104065
6	19.077199713328095	41.21960011844528	21.66175290426961	18.041447263957018
7	78.75988653285783	7.230097116544145	9.56853303807844	4.44148331251958
8	77.63744587351246	7.253271192477867	9.52132658710234	5.587956346907333
9	72.20376879138611	7.8367000115870376	11.593904359730322	8.36562683729653
10	34.890073341658834	27.675640183847673	18.080499873400882	19.353786601092615
11	28.563550611752692	27.264944060355596	24.36732626953167	19.804179058360045
12	23.972937829104065	24.61837875881366	29.824177427591742	21.584505984490534
13	21.004724936593153	31.09403095884885	29.477210012917404	18.424034091640596
14	21.033263381955976	30.16256185117952	27.202931949755172	21.601242817109334
15	19.212381822941477	27.190701187456817	32.98314729700153	20.613769692600172
16	20.568923564172877	25.247082855904456	30.447088005699108	23.73690557422356
17	19.97884292697162	25.579459185731636	28.70667198812114	25.735025899175607
18	21.70166381282213	25.792961089009907	28.455404924061988	24.049970174105976
19	23.44165068084577	28.03312176260305	26.263309000553605	22.26191855599758
20	25.305232620515923	25.617868070844008	26.579592222093478	22.49730708654659
21	24.65828966736618	28.76889867349873	26.32210248949656	20.25070916963853
22	21.589441204365308	24.893892772692354	26.34484741587596	27.171818607066378
23	21.51412545758071	28.330736978529647	26.17404589325334	23.9810916706363
24	22.485505473802565	25.512511855256438	28.1846115552809	23.817371115660098
25	22.134890287916438	28.637149760319975	25.396426900810663	23.831533050952928
26	20.877911243289173	27.73078590157884	27.192417785674127	24.198885069457855
27	22.721323153905903	28.51784618421674	25.5880421768182	23.172788485059158
28	20.412069402065928	26.874847115471272	29.340525879863872	23.372557602598928
29	23.10884520146426	26.86755157304769	25.950244400671192	24.07335882481686
30	22.409975152240804	28.97016981447865	26.43260849973607	22.187246533544474
31	22.907359485707175	27.638733322175447	24.78660538411031	24.66730180800707
32	23.872302258614102	27.862534814757595	25.954750470991634	22.310412455636666
33	22.523914358914936	27.726494406035556	24.588767439565014	25.16082379548449
34	22.117080581411816	26.335406125680738	27.228680923014863	24.318832369892583
35	22.4410884949296	25.494487573974656	25.716572468339493	26.347851462756257
36	25.053107257348113	27.797304082499714	25.04087649504976	22.108712165102418
37	21.771829764954788	27.354850891987347	27.541745522897276	23.331573820160585
38	21.780198181264186	27.54560786888623	27.120535235324155	23.553658714525426
39	23.826383256300986	24.472467910342075	26.272321141194492	25.428827692162443
40	23.729395457022818	24.479334303211324	27.545393294109065	24.245876945656793
41	21.623344019157237	25.04216394371274	26.44505383681159	26.889438200318427
42	23.388006986554743	25.338920860530685	27.02504945948614	24.248022693428435
43	23.573185019247358	23.79033469373742	28.20606903299731	24.430411254017912
44	22.71788995747128	24.99259717018784	27.83764414060656	24.451868731734322
45	22.699221951858004	23.79097841806891	26.97612641029272	26.53367321978036
46	24.16970289976354	24.91620854951742	26.22382724155541	24.69026130916363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3403.0
1	2947.0
2	2491.0
3	3084.0
4	3677.0
5	2566.5
6	1456.0
7	1456.0
8	1493.0
9	1530.0
10	1553.0
11	1576.0
12	1590.0
13	1604.0
14	1565.0
15	1526.0
16	1505.0
17	1484.0
18	1388.0
19	1292.0
20	1292.0
21	1379.5
22	1467.0
23	1900.0
24	2333.0
25	2662.5
26	2992.0
27	3197.5
28	3403.0
29	4305.5
30	5208.0
31	5208.0
32	6287.0
33	7366.0
34	8345.5
35	9325.0
36	10068.5
37	10812.0
38	12759.0
39	14706.0
40	18347.5
41	21989.0
42	26922.5
43	31856.0
44	31856.0
45	34539.5
46	37223.0
47	43318.0
48	49413.0
49	47934.0
50	46455.0
51	43112.0
52	39769.0
53	35182.5
54	30596.0
55	28238.0
56	25880.0
57	25880.0
58	24552.5
59	23225.0
60	21869.5
61	20514.0
62	18699.5
63	16885.0
64	14963.0
65	13041.0
66	11637.5
67	10234.0
68	8943.0
69	7652.0
70	7652.0
71	6482.0
72	5312.0
73	4360.0
74	3408.0
75	2882.5
76	2357.0
77	1798.0
78	1239.0
79	983.0
80	727.0
81	727.0
82	557.5
83	388.0
84	274.5
85	161.0
86	107.5
87	54.0
88	28.0
89	2.0
90	2.5
91	3.0
92	2.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	466038.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.57727809347029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75430899430799	30.377266319398753
2	9.063894138481775	7.1744851785693715
3	3.345888531530624	3.9726348264642137
4	1.7569248909170092	2.781372199886497
5	1.170110798089612	2.315490022808252
6	0.8426220747885066	2.0009212908960987
7	0.7002698534113986	1.940034231025562
8	0.5826699997511531	1.844839409349892
9	0.5204769640715156	1.8539155393473168
>10	5.155843175660202	37.08582727337701
>50	0.06245642935980262	1.736102318212527
>100	0.03584455945866919	2.535693827900597
>500	0.004344795085899297	1.0775140424909462
>1k	0.004344795085899297	3.3039035202729576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2972	0.6377162377316871	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG	2665	0.5718417811423103	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTA	2233	0.4791454774074217	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCG	2177	0.4671292898862324	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1592	0.3416030452452375	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	1440	0.30898767911629527	No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	1194	0.25620228393392813	No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTT	1098	0.23560310532617512	No Hit
TGATGAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACC	920	0.19740879499096642	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	724	0.155352138666804	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	657	0.1409756285968097	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGT	575	0.12338049686935401	No Hit
TCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	541	0.1160849544457748	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	538	0.11544123011428253	No Hit
TGATGAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGT	531	0.11393920667413387	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA	527	0.11308090756547749	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGT	482	0.10342504259309326	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.145747771640939E-4	0.0	0.0	0.2770160373188452	0.0
2	2.145747771640939E-4	0.0	0.0	1.1273758792201494	0.0
3	2.145747771640939E-4	0.0	0.0	1.4556752882812132	0.0
4	2.145747771640939E-4	0.0	0.0	1.9391122612319167	0.0
5	2.145747771640939E-4	0.0	0.0	3.433840158956995	0.0
6	2.145747771640939E-4	0.0	0.0	4.67107832408516	0.0
7	2.145747771640939E-4	0.0	0.0	5.546972564468992	0.0
8	2.145747771640939E-4	0.0	0.0	7.063587089464807	0.0
9	2.145747771640939E-4	0.0	0.0	7.662465292529793	0.0
10	2.145747771640939E-4	0.0	0.0	9.232294362262305	0.0
11	2.145747771640939E-4	0.0	0.0	10.542058802071933	0.0
12	2.145747771640939E-4	0.0	0.0	12.135061947738167	0.0
13	2.145747771640939E-4	0.0	0.0	12.621288392792003	0.0
14	2.145747771640939E-4	0.0	0.0	13.009025015127522	0.0
15	2.145747771640939E-4	0.0	0.0	13.401696857337814	0.0
16	2.145747771640939E-4	0.0	0.0	14.403546491916968	0.0
17	2.145747771640939E-4	0.0	0.0	15.266566245670953	0.0
18	2.145747771640939E-4	0.0	0.0	16.338367257605604	0.0
19	2.145747771640939E-4	0.0	0.0	16.878666546504792	0.0
20	2.145747771640939E-4	0.0	0.0	17.361459795124002	0.0
21	2.145747771640939E-4	0.0	0.0	17.89854046236573	0.0
22	2.145747771640939E-4	0.0	0.0	18.43090048450985	0.0
23	2.145747771640939E-4	0.0	0.0	19.102304962256298	0.0
24	2.145747771640939E-4	0.0	0.0	19.578660967560584	0.0
25	2.145747771640939E-4	0.0	0.0	19.98141782429759	0.0
26	2.145747771640939E-4	0.0	0.0	20.344478347259237	0.0
27	4.291495543281878E-4	0.0	0.0	20.731356670486097	0.0
28	4.291495543281878E-4	0.0	0.0	21.115016372055496	0.0
29	4.291495543281878E-4	0.0	0.0	21.505757041271313	0.0
30	4.291495543281878E-4	0.0	0.0	21.99949360352589	0.0
31	4.291495543281878E-4	0.0	0.0	22.409116853132147	0.0
32	4.291495543281878E-4	0.0	0.0	22.808869662988855	0.0
33	4.291495543281878E-4	0.0	0.0	23.195104261884225	0.0
34	4.291495543281878E-4	0.0	0.0	23.59120930052914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCACG	30	4.8612765E-6	40.000004	1
ACCGTAG	30	4.8612765E-6	40.000004	26
TAGTACG	30	4.8612765E-6	40.000004	1
GTACGAG	30	4.8612765E-6	40.000004	1
TCGTTTA	20	0.0012550006	40.0	38
CTCGTCA	20	0.0012550006	40.0	36
CACGACT	20	0.0012550006	40.0	12
TCTAGCG	20	0.0012550006	40.0	1
CGTTAGG	45	1.2187229E-9	40.0	2
CTACGCG	25	7.787513E-5	40.0	1
TTGACGG	20	0.0012550006	40.0	2
ACTACGG	40	1.924127E-8	40.0	2
CTCATAA	20	0.0012550006	40.0	18
ACAGACG	20	0.0012550006	40.0	1
AATAGCG	25	7.787513E-5	40.0	1
ATCGCCA	20	0.0012550006	40.0	24
TGCACCA	25	7.787513E-5	40.0	28
GCATCGT	25	7.787513E-5	40.0	35
ACGGGTA	65	0.0	39.999996	5
TTAACGG	90	0.0	37.77778	2
>>END_MODULE