FastQCFastQC Report
Sat 18 Jun 2016
SRR3553733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139603
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233002.0445716622367613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31790.2789567946030328No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC24020.21077515590955798No Hit
CGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC23520.20638766307214004No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC21510.18874994186571992No Hit
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTT15490.13592452810320788No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT15450.13557352867621444No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATG14190.1245170467259212No Hit
CGTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT13120.11512781205384683No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.1104770696461838No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT12400.10880982236796499No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG12130.10644057623575928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG304.8674774E-640.0000041
TATCACG200.001255764240.01
TCTAGCG353.056357E-740.01
ATCGACG200.001255764240.01
CGTTTTT135450.039.3798451
CGTTATT6900.038.5507241
GCCGATA1100.036.3636369
CGTAAGG1050.036.190482
TGTAGCG503.425157E-936.01
ACATGCG455.4424163E-835.5555571
TACGGGT1250.035.24
ACTCGCG408.6998443E-735.01
GTTTTTT153000.034.9019622
CGGGTTC750.034.6666686
CTCGTAG351.4010571E-534.2857131
CGGGAAT4850.033.8144346
TTGTCGG302.2782487E-433.3333362
AATTGCG302.2782487E-433.3333361
TTGCACG605.511538E-1033.3333361
CGATACG1200.033.33333610
Produced by FastQC (version 0.11.5)