##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1139603
Sequences flagged as poor quality	0
Sequence length	46
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.534343977683456	31.0	31.0	34.0	30.0	34.0
2	31.723695006067903	31.0	31.0	34.0	30.0	34.0
3	31.327729919980907	31.0	31.0	34.0	28.0	34.0
4	35.325041264370135	37.0	35.0	37.0	33.0	37.0
5	33.50731087931499	37.0	35.0	37.0	30.0	37.0
6	34.3866978237158	37.0	35.0	37.0	30.0	37.0
7	35.47923970014119	37.0	35.0	37.0	32.0	37.0
8	35.62336357485896	37.0	35.0	37.0	35.0	37.0
9	37.41094223163681	39.0	37.0	39.0	35.0	39.0
10	36.71380384221523	39.0	37.0	39.0	32.0	39.0
11	36.478471011396074	39.0	35.0	39.0	32.0	39.0
12	36.736055450889474	39.0	35.0	39.0	32.0	39.0
13	32.92981064458412	38.0	35.0	39.0	0.0	39.0
14	35.28024320750296	39.0	35.0	41.0	17.0	41.0
15	36.759307407930656	39.0	35.0	41.0	30.0	41.0
16	37.38768939709706	39.0	36.0	41.0	32.0	41.0
17	37.577442319825415	39.0	36.0	41.0	32.0	41.0
18	37.566865829591535	39.0	36.0	40.0	33.0	41.0
19	37.415041027445525	39.0	36.0	40.0	32.0	41.0
20	37.26780291031175	39.0	35.0	40.0	32.0	41.0
21	37.16733195683058	39.0	35.0	40.0	32.0	41.0
22	37.156279862373125	39.0	35.0	40.0	32.0	41.0
23	37.014763913397914	39.0	35.0	40.0	32.0	41.0
24	36.93162443412311	38.0	35.0	40.0	32.0	41.0
25	36.73314917563397	38.0	35.0	40.0	31.0	41.0
26	36.70699884082439	38.0	35.0	40.0	31.0	41.0
27	36.27266688487131	38.0	35.0	40.0	31.0	41.0
28	36.296283003818	38.0	35.0	40.0	30.0	41.0
29	36.31867501226304	38.0	35.0	40.0	30.0	41.0
30	35.73749718103585	38.0	35.0	40.0	30.0	41.0
31	35.68042291920958	38.0	35.0	40.0	28.0	41.0
32	35.357401656541796	38.0	34.0	40.0	26.0	41.0
33	34.9982081479252	38.0	34.0	40.0	24.0	41.0
34	34.64915238025874	38.0	34.0	40.0	21.0	41.0
35	34.407512089736514	38.0	34.0	40.0	20.0	41.0
36	34.20800928042485	38.0	33.0	40.0	18.0	41.0
37	34.12659145333945	38.0	33.0	40.0	18.0	41.0
38	34.00412161077147	38.0	33.0	40.0	18.0	41.0
39	33.92372168202436	38.0	33.0	40.0	16.0	41.0
40	33.83218717395444	38.0	33.0	40.0	16.0	41.0
41	33.67756139638102	38.0	33.0	40.0	15.0	41.0
42	33.562294062054946	38.0	33.0	40.0	15.0	41.0
43	33.43431440598173	38.0	33.0	40.0	15.0	41.0
44	33.34093802841867	37.0	33.0	40.0	15.0	41.0
45	33.30246498122592	37.0	33.0	40.0	15.0	41.0
46	32.28973510950743	36.0	30.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	13.0
11	14.0
12	8.0
13	17.0
14	14.0
15	44.0
16	60.0
17	153.0
18	363.0
19	731.0
20	1382.0
21	2431.0
22	3819.0
23	6063.0
24	8983.0
25	13709.0
26	20337.0
27	28710.0
28	34377.0
29	34537.0
30	34633.0
31	38387.0
32	46115.0
33	58805.0
34	78905.0
35	100831.0
36	119434.0
37	155703.0
38	191391.0
39	159621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.858468256050575	20.446067621794608	19.693612600177428	30.00185152197739
2	35.63126808195486	23.030564152604022	20.226429730353466	21.111738035087658
3	24.402796412434856	23.6570981297873	32.03922769596079	19.900877761817053
4	21.506085891314783	24.99510794548628	31.752110164680158	21.746695998518785
5	18.46063936300624	32.13259354354104	28.835041676794464	20.571725416658257
6	17.439143280598596	35.697870223226865	30.567750348147555	16.29523614802699
7	75.00752455021617	4.042460400683396	17.317258729575123	3.6327563195253085
8	74.97672435049749	3.6668032639436716	17.25908057455096	4.097391811007869
9	68.73999103196465	6.069131092143492	19.97757113661512	5.2133067392767485
10	28.32354776180828	26.97878120713968	27.775198906987786	16.922472124064257
11	23.541092819165975	25.29881019969235	34.04545267079852	17.114644310343163
12	19.398158832505704	22.83154747749874	38.53280484519609	19.23748884479946
13	18.414833937783598	29.455959663145848	36.28447801558964	15.844728383480913
14	19.04944090178773	25.53766530976138	36.994198856970364	18.418694931480527
15	18.25855144291477	24.810218997317488	38.792983170454974	18.13824638931277
16	19.569007803594758	24.75283059100406	37.37906972866867	18.29909187673251
17	19.45782873509459	24.759236330546692	36.04448215738288	19.738452776975844
18	20.874637922153592	23.502921631480437	36.34318266975429	19.27925777661168
19	20.77907832815463	26.161917790669207	34.745696527650416	18.313307353525747
20	22.553994680603683	24.056623227562582	34.41821406226555	18.97116802956819
21	22.226687714932307	25.65059937539652	34.85626134715335	17.266451562517823
22	21.6683353764425	23.111645020239504	34.57712905283682	20.642890550481177
23	19.38069661101278	25.838998317835244	34.58265729381197	20.197647777340002
24	19.146053494067672	24.33566777202236	36.34397241846503	20.17430631544494
25	19.903598007376253	25.58057498971133	34.39890909378091	20.116917909131512
26	19.15956697200692	25.693772304916713	35.245958460972815	19.900702262103557
27	20.05619500826165	25.135595466140405	34.17225121379989	20.635958311798056
28	19.28733076343253	25.571449004609498	34.88688604715853	20.254334184799443
29	19.5215351310939	24.942458031437265	33.60608913805948	21.92991769940936
30	19.965022907100106	25.49449238024119	33.51403953833045	21.026445174328252
31	19.688347608772528	25.480715652731696	32.56835933215339	22.262577406342384
32	19.451510745408708	26.23141567721391	32.47332623729492	21.843747340082466
33	19.236786845945474	25.15340868706032	31.26071096688935	24.34909350010486
34	18.950195813805333	26.356546972937068	31.878469958397794	22.814787254859805
35	19.006443471981033	25.0606570884773	31.781594116547605	24.15130532299406
36	19.99143561398136	27.01133640399332	30.275280075605277	22.72194790642004
37	20.431237896004134	26.183943004713043	31.062396290638056	22.322422808644767
38	19.930712713111497	26.240278412745493	31.461921388413334	22.36708748572968
39	21.618932207093174	24.600847839115904	30.619786013199334	23.16043394059159
40	21.790395427179465	24.550830420769337	32.32845122380338	21.33032292824782
41	20.37595548625267	25.226943067015444	31.588983180984957	22.80811826574693
42	21.629023440619232	26.31776153625429	30.527824163327054	21.52539085979942
43	20.64534754647013	25.154022936057558	31.666027555210015	22.534601962262297
44	21.322600940853963	25.954564879172835	29.892164201041943	22.830669978931258
45	20.932289578037263	24.378928451399304	30.842758399196914	23.84602357136652
46	21.114458280646854	24.937105290175616	29.990180791029857	23.958255638147673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3514.0
1	2939.5
2	2365.0
3	22807.0
4	43249.0
5	29897.5
6	16546.0
7	16546.0
8	15401.5
9	14257.0
10	13857.0
11	13457.0
12	12980.0
13	12503.0
14	11839.0
15	11175.0
16	10555.0
17	9935.0
18	9472.5
19	9010.0
20	9010.0
21	8567.5
22	8125.0
23	8488.5
24	8852.0
25	9314.0
26	9776.0
27	10806.5
28	11837.0
29	14110.5
30	16384.0
31	16384.0
32	18620.0
33	20856.0
34	23398.0
35	25940.0
36	29521.5
37	33103.0
38	37056.5
39	41010.0
40	47371.5
41	53733.0
42	60841.5
43	67950.0
44	67950.0
45	77750.5
46	87551.0
47	97286.0
48	107021.0
49	108635.0
50	110249.0
51	102195.0
52	94141.0
53	82616.5
54	71092.0
55	64118.5
56	57145.0
57	57145.0
58	52258.5
59	47372.0
60	42336.5
61	37301.0
62	33278.5
63	29256.0
64	24795.5
65	20335.0
66	17231.0
67	14127.0
68	11990.0
69	9853.0
70	9853.0
71	8462.5
72	7072.0
73	5968.0
74	4864.0
75	4173.0
76	3482.0
77	2960.0
78	2438.0
79	1949.5
80	1461.0
81	1461.0
82	1100.5
83	740.0
84	528.0
85	316.0
86	224.0
87	132.0
88	90.5
89	49.0
90	30.5
91	12.0
92	11.5
93	11.0
94	11.0
95	6.5
96	2.0
97	3.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1139603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.02902014678213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02476909477876	33.03267069981692
2	9.161480493289362	8.067420184267778
3	4.075748781323083	5.3835367561829015
4	2.429918900239456	4.279477928547588
5	1.693109676669956	3.7272980032406644
6	1.280503964104955	3.382760090016687
7	1.040873486295431	3.2080047788847024
8	0.8174436785709585	2.879299535412834
9	0.6949618516184827	2.753864042953967
>10	3.6935010625873974	24.954621875347943
>50	0.05763510882168143	1.7202116050803782
>100	0.025908536334582455	2.1371756274521916
>500	0.001450877878064654	0.464412916724238
>1k	0.0024872192195394068	1.882930184863667
>5k	0.0	0.0
>10k+	2.0726826829495055E-4	2.1263157712074565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23300	2.0445716622367613	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3179	0.2789567946030328	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	2402	0.21077515590955798	No Hit
CGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC	2352	0.20638766307214004	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	2151	0.18874994186571992	No Hit
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTT	1549	0.13592452810320788	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	1545	0.13557352867621444	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATG	1419	0.1245170467259212	No Hit
CGTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT	1312	0.11512781205384683	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1259	0.1104770696461838	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	1240	0.10880982236796499	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	1213	0.10644057623575928	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.156984493722814	0.0
2	0.0	0.0	0.0	0.5138631611183895	0.0
3	0.0	0.0	0.0	0.8997870310976718	0.0
4	0.0	0.0	0.0	1.2768481655453698	0.0
5	0.0	0.0	0.0	2.0157897092232995	0.0
6	0.0	0.0	0.0	3.255431935507365	0.0
7	0.0	0.0	0.0	4.161185956863926	0.0
8	0.0	0.0	0.0	5.667412247949505	0.0
9	0.0	0.0	0.0	6.450228720001615	0.0
10	0.0	0.0	0.0	7.597119347702665	0.0
11	0.0	0.0	0.0	8.612736189708171	0.0
12	0.0	0.0	0.0	9.436619594718511	0.0
13	0.0	0.0	0.0	9.929773789644289	0.0
14	0.0	0.0	0.0	10.192321361035377	0.0
15	0.0	0.0	0.0	10.461625671396092	0.0
16	0.0	0.0	0.0	11.017608763753692	0.0
17	0.0	0.0	0.0	11.628962015719509	0.0
18	0.0	0.0	0.0	12.375976546218288	8.774985674835885E-5
19	0.0	0.0	0.0	12.750843934247277	8.774985674835885E-5
20	0.0	0.0	0.0	13.128782567262459	8.774985674835885E-5
21	0.0	0.0	0.0	13.588679566480607	8.774985674835885E-5
22	0.0	0.0	0.0	14.061037045357024	8.774985674835885E-5
23	0.0	0.0	0.0	14.581042696447797	8.774985674835885E-5
24	8.774985674835885E-5	0.0	0.0	14.990483528035641	8.774985674835885E-5
25	8.774985674835885E-5	0.0	0.0	15.36157767222445	8.774985674835885E-5
26	8.774985674835885E-5	0.0	0.0	15.715911593774322	8.774985674835885E-5
27	8.774985674835885E-5	0.0	0.0	16.091393230800552	8.774985674835885E-5
28	8.774985674835885E-5	0.0	0.0	16.482055593044244	8.774985674835885E-5
29	8.774985674835885E-5	0.0	0.0	16.9094851452655	8.774985674835885E-5
30	1.754997134967177E-4	0.0	0.0	17.420540310967944	8.774985674835885E-5
31	1.754997134967177E-4	0.0	0.0	17.855428601012807	8.774985674835885E-5
32	1.754997134967177E-4	0.0	0.0	18.28198065466658	8.774985674835885E-5
33	1.754997134967177E-4	0.0	0.0	18.701600469637235	8.774985674835885E-5
34	1.754997134967177E-4	0.0	0.0	19.117096041340712	8.774985674835885E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	30	4.8674774E-6	40.000004	1
TATCACG	20	0.0012557642	40.0	1
TCTAGCG	35	3.056357E-7	40.0	1
ATCGACG	20	0.0012557642	40.0	1
CGTTTTT	13545	0.0	39.379845	1
CGTTATT	690	0.0	38.550724	1
GCCGATA	110	0.0	36.363636	9
CGTAAGG	105	0.0	36.19048	2
TGTAGCG	50	3.425157E-9	36.0	1
ACATGCG	45	5.4424163E-8	35.555557	1
TACGGGT	125	0.0	35.2	4
ACTCGCG	40	8.6998443E-7	35.0	1
GTTTTTT	15300	0.0	34.901962	2
CGGGTTC	75	0.0	34.666668	6
CTCGTAG	35	1.4010571E-5	34.285713	1
CGGGAAT	485	0.0	33.814434	6
TTGTCGG	30	2.2782487E-4	33.333336	2
AATTGCG	30	2.2782487E-4	33.333336	1
TTGCACG	60	5.511538E-10	33.333336	1
CGATACG	120	0.0	33.333336	10
>>END_MODULE