FastQCFastQC Report
Sat 18 Jun 2016
SRR3553732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555378
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38520.693581668701317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23980.43177799624760077No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCG10420.18761996334028355No Hit
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCT8640.1555697200825384No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTT8160.1469269578557307No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCT8090.1456665550309879No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCCTGATACGTCGT7300.13144200886603358No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGATACGTCGTA5850.1053336646392187No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG200.001255208340.0000041
CTATACG200.001255208340.0000041
TTCACCG401.9252184E-840.00000423
GAATAAC200.001255208340.0000049
CTACGCG200.001255208340.0000041
TCGTAAG200.001255208340.0000041
ACGTCCT200.001255208340.00000423
CCGTTAG200.001255208340.0000041
GAGTACG257.789444E-540.01
TAGAACG257.789444E-540.01
ATATACC257.789444E-540.019
ACTACGG304.8629645E-640.02
ATAACGG353.0525734E-740.02
TAGTAGG1100.038.1818162
CTAACGG700.037.1428572
AGTACGG700.037.1428572
TACGGGT650.036.9230774
CGTTAGG552.1645974E-1036.3636362
CGGGATC1050.036.190486
GCGTAAG503.417881E-936.01