##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801266
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.583939665479377	31.0	31.0	34.0	30.0	34.0
2	31.75379711606383	31.0	31.0	34.0	30.0	34.0
3	31.60597229883709	31.0	31.0	34.0	30.0	34.0
4	35.458666660010536	37.0	35.0	37.0	33.0	37.0
5	33.60110000923539	37.0	35.0	37.0	30.0	37.0
6	34.43025162680059	37.0	35.0	37.0	30.0	37.0
7	35.41642974992075	37.0	35.0	37.0	32.0	37.0
8	35.504220820551474	37.0	35.0	37.0	33.0	37.0
9	37.28148704674852	39.0	37.0	39.0	34.0	39.0
10	36.666560168533294	39.0	37.0	39.0	32.0	39.0
11	36.39006771783652	38.0	35.0	39.0	32.0	39.0
12	36.58894549375613	39.0	35.0	39.0	32.0	39.0
13	32.96908891678918	38.0	35.0	39.0	10.0	39.0
14	35.22494028200373	39.0	35.0	40.0	17.0	41.0
15	36.63476797967217	39.0	35.0	41.0	30.0	41.0
16	37.24562130428597	39.0	36.0	41.0	32.0	41.0
17	37.4182780749464	39.0	36.0	40.0	32.0	41.0
18	37.49839004774944	39.0	36.0	40.0	32.0	41.0
19	37.4448622554807	39.0	36.0	40.0	32.0	41.0
20	37.405955076092084	39.0	36.0	40.0	32.0	41.0
21	37.29616881285366	39.0	35.0	40.0	32.0	41.0
22	37.30374806868131	39.0	35.0	40.0	32.0	41.0
23	37.16702817790846	39.0	35.0	40.0	32.0	41.0
24	37.13994229132398	39.0	35.0	40.0	32.0	41.0
25	36.93545713907741	39.0	35.0	40.0	31.0	41.0
26	36.88839286828594	39.0	35.0	40.0	31.0	41.0
27	36.475883164891556	39.0	35.0	40.0	31.0	41.0
28	36.49333554649767	39.0	35.0	40.0	30.0	41.0
29	36.53093479568583	38.0	35.0	40.0	30.0	41.0
30	36.04723150614153	38.0	35.0	40.0	30.0	41.0
31	36.083645131579274	38.0	35.0	40.0	30.0	41.0
32	35.98706297284547	38.0	35.0	40.0	29.0	41.0
33	35.86224924057679	38.0	35.0	40.0	29.0	41.0
34	35.79102694985186	38.0	35.0	40.0	29.0	41.0
35	35.64582298512604	38.0	35.0	40.0	28.0	41.0
36	35.521216425007424	38.0	35.0	40.0	27.0	41.0
37	35.45426362780899	38.0	34.0	40.0	27.0	41.0
38	35.3308601637908	38.0	34.0	40.0	27.0	41.0
39	35.264137003192445	38.0	34.0	40.0	26.0	41.0
40	35.1674175617086	38.0	34.0	40.0	26.0	41.0
41	34.97451782554108	38.0	34.0	40.0	25.0	41.0
42	34.87883549283259	38.0	34.0	40.0	25.0	41.0
43	34.72555181425394	38.0	34.0	40.0	24.0	41.0
44	34.6168525807909	37.0	33.0	40.0	24.0	41.0
45	34.5271520319095	37.0	33.0	40.0	23.0	41.0
46	33.30583351845704	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	11.0
11	15.0
12	15.0
13	9.0
14	16.0
15	26.0
16	51.0
17	99.0
18	250.0
19	436.0
20	842.0
21	1363.0
22	2109.0
23	3197.0
24	4654.0
25	6484.0
26	8955.0
27	12047.0
28	15099.0
29	17707.0
30	21642.0
31	26380.0
32	33775.0
33	44310.0
34	61131.0
35	77307.0
36	90142.0
37	116645.0
38	140621.0
39	115913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.99337049119768	21.682936752589026	20.504676349676636	25.819016406536655
2	28.631690350021096	25.734899521507216	22.116875045240906	23.51653508323079
3	27.39277593208747	26.29725958670404	25.279245593847737	21.030718887360752
4	24.50534529107687	26.912161504419256	24.76842896116895	23.814064243334922
5	19.944812334480684	34.25117251948791	21.913446970169705	23.8905681758617
6	19.755861349414552	38.108817795838085	23.74242760830985	18.392893246437513
7	79.43579285780253	5.789587977026356	10.34662646362132	4.4279927015497975
8	78.63680725252287	5.538235741938383	10.520101938682036	5.3048550668567
9	72.4160266378456	7.300447042555157	13.768336607318918	6.515189712280317
10	33.90721682936753	26.052147476618252	20.532008097186203	19.50862759682802
11	27.560760097146265	26.302501291705877	26.185186941664817	19.95155166948304
12	22.602231967910782	23.525021653233757	31.773967696120884	22.098778682734572
13	20.40271270714095	29.316731272760855	31.279125783447693	19.001430236650503
14	20.071986082025195	27.1374300169981	30.97248604084037	21.818097860136334
15	20.03354691201174	25.235439916332403	33.53043808173565	21.2005750899202
16	21.482753542518964	24.4168603185459	32.08035783372812	22.02002830520701
17	20.859614659800865	25.10477169878667	29.752291997913304	24.28332164349916
18	22.18863648276602	24.438575953553503	30.343606243120263	23.029181320560212
19	23.505427660726898	27.051940304468182	28.123619372343267	21.319012662461652
20	25.16954419630934	24.93791075622827	27.214308357025008	22.678236690437384
21	24.763561663667247	26.593415919307695	28.040126499814043	20.602895917211015
22	23.188554113115995	23.876839900856893	27.453679552108788	25.48092643391832
23	21.8197202926369	26.742929314360026	27.491245104621935	23.946105288381137
24	21.628273257569898	25.00680173625238	29.997653712999178	23.367271293178547
25	23.57431864075101	25.770593036519706	27.35583439207454	23.29925393065474
26	21.920685515172238	26.370643456729724	27.981968534793687	23.726702493304348
27	23.10194117808568	24.966240923738184	28.116006419840602	23.815811478335533
28	21.9254280101739	25.787316571525558	29.531017165335854	22.756238252964682
29	23.16259519310691	25.43375108890181	27.548404649641945	23.855249068349337
30	23.77225540582029	24.999563191249845	28.235317609882358	22.9928637930475
31	23.870100565854536	25.48978741142143	27.100613279485213	23.539498743238823
32	24.58559329860496	25.860700441551245	27.201703304520596	22.3520029553232
33	22.451844955358148	25.410163416393555	27.12844423699495	25.00954739125334
34	21.837192642643018	26.094954734133236	28.334535597417087	23.73331702580666
35	22.948808510532082	25.887158571560505	27.505098182126787	23.658934735780626
36	23.51353982322974	26.809324244383266	26.01745238160611	23.659683550780887
37	22.444356805355525	27.384289362084502	27.158521639505484	23.012832193054493
38	22.801042350480365	27.071534296975035	26.922520111922882	23.204903240621714
39	22.763601600467258	25.119872801291955	25.590503028956675	26.52602256928411
40	24.339607571018863	23.993405435897692	28.740018920059008	22.92696807302444
41	21.916192625170666	24.470525393564685	27.8760112122566	25.73727076900805
42	23.692756213292466	25.093789078782823	27.392151919587253	23.821302788337455
43	22.351129337822893	23.340813163169287	29.84177539044462	24.4662821085632
44	23.15385901810385	24.345473288520918	28.09703643983396	24.40363125354127
45	23.023565208058248	23.686016878290104	28.411039529943867	24.87937838370778
46	23.646704090776346	24.083887248429363	27.168755444509063	25.10065321628523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3870.0
1	3244.5
2	2619.0
3	8721.5
4	14824.0
5	9935.5
6	5047.0
7	5047.0
8	4634.5
9	4222.0
10	4174.5
11	4127.0
12	4076.0
13	4025.0
14	3902.5
15	3780.0
16	3415.0
17	3050.0
18	2953.0
19	2856.0
20	2856.0
21	2930.0
22	3004.0
23	3535.5
24	4067.0
25	4640.0
26	5213.0
27	6047.5
28	6882.0
29	8287.5
30	9693.0
31	9693.0
32	10804.0
33	11915.0
34	13577.0
35	15239.0
36	17295.5
37	19352.0
38	21735.5
39	24119.0
40	28056.0
41	31993.0
42	37149.5
43	42306.0
44	42306.0
45	49678.5
46	57051.0
47	64831.0
48	72611.0
49	75688.0
50	78765.0
51	73459.0
52	68153.0
53	60391.0
54	52629.0
55	49645.0
56	46661.0
57	46661.0
58	44368.5
59	42076.0
60	39615.5
61	37155.0
62	34449.0
63	31743.0
64	28735.5
65	25728.0
66	22942.0
67	20156.0
68	17656.5
69	15157.0
70	15157.0
71	13116.5
72	11076.0
73	9353.5
74	7631.0
75	6484.0
76	5337.0
77	4369.5
78	3402.0
79	2607.0
80	1812.0
81	1812.0
82	1388.5
83	965.0
84	770.5
85	576.0
86	404.5
87	233.0
88	150.5
89	68.0
90	54.0
91	40.0
92	32.5
93	25.0
94	25.0
95	17.5
96	10.0
97	5.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	801266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.76107585453798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52608643164787	34.70151035472458
2	8.410750369800626	7.529484705924585
3	3.2462590043878072	4.359181366166387
4	1.816137755307147	3.2516911931037416
5	1.2850225544571179	2.8759496017423607
6	1.0505651243033853	2.821465513144557
7	0.8598380320067267	2.6941092761288825
8	0.7439293305452035	2.6639261755959613
9	0.6941219539462186	2.796268088959813
>10	4.288798279767057	29.656074127907605
>50	0.05069229931269623	1.5686870610654862
>100	0.022620644657213272	1.954416057081525
>500	0.0035429925364187337	1.0637605653256859
>1k	0.0013626894370841283	1.07791150862589
>5k	2.725378874168257E-4	0.9855644045030063
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8079	1.0082793978528979	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3504	0.43730796015305784	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	1521	0.1898246025664386	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	1517	0.18932539256626388	No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1278	0.15949759505582414	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	1016	0.12679934004437976	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	949	0.11843757254145315	No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	936	0.1168151400408853	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	833	0.10396048253638616	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.496050000873617E-4	0.0	0.0	0.18845177506595812	0.0
2	2.496050000873617E-4	0.0	0.0	0.6373663677230782	0.0
3	2.496050000873617E-4	0.0	0.0	1.0287470078600613	0.0
4	2.496050000873617E-4	0.0	0.0	1.3935447154877407	0.0
5	2.496050000873617E-4	0.0	0.0	2.244073753285426	0.0
6	2.496050000873617E-4	0.0	0.0	3.617150858766003	0.0
7	2.496050000873617E-4	0.0	0.0	4.578754121602564	0.0
8	2.496050000873617E-4	0.0	0.0	6.31762735471117	0.0
9	3.7440750013104264E-4	0.0	0.0	7.109374414988281	0.0
10	3.7440750013104264E-4	0.0	0.0	8.3068544029074	0.0
11	3.7440750013104264E-4	0.0	0.0	9.439437090803803	0.0
12	3.7440750013104264E-4	0.0	0.0	10.374582223631103	0.0
13	3.7440750013104264E-4	0.0	0.0	10.857318293800061	0.0
14	3.7440750013104264E-4	0.0	0.0	11.101806391385633	0.0
15	3.7440750013104264E-4	0.0	0.0	11.397338711489068	0.0
16	3.7440750013104264E-4	0.0	0.0	11.998637156699523	0.0
17	3.7440750013104264E-4	0.0	0.0	12.662586456931905	0.0
18	3.7440750013104264E-4	0.0	0.0	13.471930669715176	0.0
19	3.7440750013104264E-4	0.0	0.0	13.883154907359105	0.0
20	3.7440750013104264E-4	0.0	0.0	14.298872035004605	0.0
21	3.7440750013104264E-4	0.0	0.0	14.772123115170244	0.0
22	3.7440750013104264E-4	0.0	0.0	15.222535337827887	0.0
23	3.7440750013104264E-4	0.0	0.0	15.75294596301353	0.0
24	3.7440750013104264E-4	0.0	0.0	16.186759453165365	0.0
25	3.7440750013104264E-4	0.0	0.0	16.548811505792084	0.0
26	4.992100001747234E-4	0.0	0.0	16.89613686341365	0.0
27	4.992100001747234E-4	0.0	0.0	17.257564903540146	0.0
28	4.992100001747234E-4	0.0	0.0	17.62860273617001	0.0
29	4.992100001747234E-4	0.0	0.0	18.032214021311276	0.0
30	4.992100001747234E-4	0.0	0.0	18.496978531473943	0.0
31	4.992100001747234E-4	0.0	0.0	18.890106406611537	0.0
32	4.992100001747234E-4	0.0	0.0	19.304700311756644	0.0
33	4.992100001747234E-4	0.0	0.0	19.707437979397604	0.0
34	4.992100001747234E-4	0.0	0.0	20.10131466953546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	0.0012555411	40.0	1
TTATCGG	20	0.0012555411	40.0	2
ACATGCG	40	1.9270374E-8	40.0	1
ATAACGG	95	0.0	37.894737	2
CGTTTTT	3585	0.0	37.433754	1
TAACGCC	65	0.0	36.923077	12
CTCGACG	65	0.0	36.923077	1
TCGTTTA	55	2.1645974E-10	36.363636	38
ACGATAG	55	2.1645974E-10	36.363636	1
CATCGTT	55	2.1645974E-10	36.363636	36
CCCTCGT	100	0.0	36.0	14
CGTGTAC	100	0.0	36.0	18
AACGGGA	440	0.0	35.909092	4
CACTTAC	710	0.0	35.774647	37
TACGGGA	230	0.0	35.652176	4
ACTACGG	85	0.0	35.29412	2
ACGGGAT	205	0.0	35.121952	5
TTAACGG	80	0.0	35.0	2
TTACCGG	40	8.6955515E-7	35.0	2
GCGTACG	40	8.6955515E-7	35.0	1
>>END_MODULE