##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553729_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679870
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56272816862047	31.0	31.0	34.0	30.0	34.0
2	31.732953358730345	31.0	31.0	34.0	30.0	34.0
3	31.619762601673848	31.0	31.0	34.0	30.0	34.0
4	35.452460029123216	37.0	35.0	37.0	33.0	37.0
5	33.60618206421816	37.0	35.0	37.0	30.0	37.0
6	34.41960668951417	37.0	35.0	37.0	30.0	37.0
7	35.40851633400503	37.0	35.0	37.0	32.0	37.0
8	35.46501242884669	37.0	35.0	37.0	33.0	37.0
9	37.235758306735114	39.0	37.0	39.0	34.0	39.0
10	36.64545133628488	39.0	35.0	39.0	32.0	39.0
11	36.36688925824055	38.0	35.0	39.0	32.0	39.0
12	36.57045611661053	39.0	35.0	39.0	32.0	39.0
13	33.03824260520393	38.0	35.0	39.0	11.0	39.0
14	35.2609837174754	39.0	35.0	40.0	18.0	41.0
15	36.60768088016827	39.0	35.0	40.0	30.0	41.0
16	37.20766175886567	39.0	36.0	41.0	32.0	41.0
17	37.35420742200715	39.0	36.0	40.0	32.0	41.0
18	37.44680453616132	39.0	36.0	40.0	32.0	41.0
19	37.39872181446453	39.0	36.0	40.0	32.0	41.0
20	37.37211672819804	39.0	36.0	40.0	32.0	41.0
21	37.29338991277744	39.0	35.0	40.0	32.0	41.0
22	37.30816185447218	39.0	35.0	40.0	32.0	41.0
23	37.189836292232336	39.0	35.0	40.0	32.0	41.0
24	37.17163575389413	39.0	35.0	40.0	32.0	41.0
25	36.96860723373586	39.0	35.0	40.0	31.0	41.0
26	36.93297542177181	39.0	35.0	40.0	31.0	41.0
27	36.5307249915425	39.0	35.0	40.0	31.0	41.0
28	36.565568417491576	39.0	35.0	40.0	30.0	41.0
29	36.63204583229147	39.0	35.0	40.0	31.0	41.0
30	36.153679379881446	38.0	35.0	40.0	30.0	41.0
31	36.20517746039684	38.0	35.0	40.0	30.0	41.0
32	36.10917822524895	38.0	35.0	40.0	30.0	41.0
33	36.0097945195405	38.0	35.0	40.0	30.0	41.0
34	35.98402488711077	38.0	35.0	40.0	29.0	41.0
35	35.86191771956403	38.0	35.0	40.0	29.0	41.0
36	35.72313236354009	38.0	35.0	40.0	28.0	41.0
37	35.67063409181167	38.0	35.0	40.0	28.0	41.0
38	35.523876623472134	38.0	34.0	40.0	27.0	41.0
39	35.496161030785295	38.0	34.0	40.0	27.0	41.0
40	35.37041640313589	38.0	34.0	40.0	27.0	41.0
41	35.20414638092576	38.0	34.0	40.0	26.0	41.0
42	35.10394193007487	38.0	34.0	40.0	26.0	41.0
43	35.00277553061615	38.0	34.0	40.0	26.0	41.0
44	34.931988468383665	38.0	34.0	40.0	26.0	41.0
45	34.873594951976116	37.0	34.0	40.0	26.0	41.0
46	33.6777045611661	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	12.0
10	14.0
11	14.0
12	12.0
13	9.0
14	10.0
15	16.0
16	43.0
17	93.0
18	194.0
19	366.0
20	639.0
21	1071.0
22	1686.0
23	2458.0
24	3552.0
25	5008.0
26	6910.0
27	9370.0
28	11828.0
29	14384.0
30	17725.0
31	22483.0
32	28627.0
33	37877.0
34	52311.0
35	65522.0
36	76451.0
37	99874.0
38	121836.0
39	99470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.664479974112695	22.024357597776046	21.656198979216615	23.65496344889464
2	27.505552532101724	26.796005118625622	22.545927897980498	23.15251445129216
3	27.375674761351437	27.036051009751866	24.592054363334167	20.996219865562534
4	24.7378175239384	27.27742068336594	24.13902657861062	23.845735214085046
5	20.63482724638534	33.84191095356465	21.328342183064407	24.1949196169856
6	19.475193787047523	39.04672952182035	23.02646094106226	18.451615750069866
7	79.51049465338961	5.627546442702281	10.390957094738699	4.4710018091694
8	78.44396722902907	5.837586597437745	10.070601732684189	5.647844440848986
9	71.50175768896995	7.274920205333372	13.53508759027461	7.6882345154220655
10	33.6334887552032	26.33091620456852	20.100901642961155	19.934693397267125
11	27.14930795593275	26.550222836718785	26.25369555944519	20.046773647903276
12	22.971744598232018	23.17472457969906	31.97537764572639	21.878153176342536
13	20.76852927765602	29.839675232029656	30.997396561107273	18.394398929207053
14	19.89512700957536	27.385529586538603	30.659832026710987	22.05951137717505
15	19.5775662994396	25.723594216541397	33.53244002529895	21.16639945872005
16	21.226116757615426	24.609263535675936	31.614573374321566	22.550046332387076
17	20.2704928883463	25.967464368188036	29.53888243340639	24.223160310059278
18	22.3272096136026	24.472766852486505	29.83290923264742	23.367114301263477
19	23.04852398252607	27.43965758159648	27.751628987894744	21.760189447982704
20	24.995660935178783	25.440746024975365	27.375527674408346	22.18806536543751
21	24.920940768088016	27.38244076073367	27.460102666686275	20.236515804492036
22	22.455028167149603	24.044596761145513	27.478341447629695	26.022033624075192
23	21.46175004044891	27.091061526468295	27.742215423536855	23.70497300954594
24	21.346433877064733	25.271007692647125	29.621839469310306	23.760718960977833
25	22.78538544133437	27.11356582876138	26.682748172444732	23.418300557459514
26	21.144483504199332	26.48168032123788	28.00388309529763	24.36995307926515
27	22.977186815126423	24.972421198170238	27.525409269419153	24.524982717284185
28	21.484254342741995	25.72668304234633	29.86012031711945	22.928942297792226
29	23.65555179666701	24.916969420624532	27.027519967052527	24.399958815655935
30	24.17491579272508	24.608675187903568	28.393222233662318	22.823186785709034
31	24.111227146366218	24.95962463412123	27.56291643990763	23.366231779604924
32	25.14657213879124	25.129657140335652	27.41171106240899	22.31205965846412
33	23.785429567417303	23.750717048847577	27.239178078162002	25.224675305573125
34	22.767440834277142	24.024298762998807	29.556827040463617	23.651433362260434
35	23.634518363804847	23.870004559695236	29.130716166325914	23.364760910174002
36	23.86029682145116	26.336505508406017	27.426419756718197	22.376777913424625
37	24.050186064983013	25.284686778354686	29.254269198523247	21.410857958139058
38	22.694338623560387	26.715107299924984	27.91974936384897	22.670804712665657
39	25.098327621457045	23.125744627649404	27.037521879182787	24.738405871710768
40	24.66206774824599	23.60054127995058	29.707444070189887	22.029946901613542
41	21.832997484813273	24.967126068218924	28.9605365731684	24.239339873799402
42	24.008413373144865	25.641225528409844	28.295409416506097	22.054951681939194
43	23.88309529763043	22.733022489593598	29.93013370203127	23.453748510744703
44	24.120934884610293	23.2795975701237	28.147734125641666	24.45173341962434
45	23.352846867783548	22.31367761483813	28.62164825628429	25.711827261094033
46	24.23860443908394	23.20458322914675	27.863120890758523	24.69369144101078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3130.0
1	2783.5
2	2437.0
3	6270.5
4	10104.0
5	6698.5
6	3293.0
7	3293.0
8	3061.5
9	2830.0
10	2812.0
11	2794.0
12	2815.5
13	2837.0
14	2721.0
15	2605.0
16	2501.5
17	2398.0
18	2290.0
19	2182.0
20	2182.0
21	2285.5
22	2389.0
23	2875.0
24	3361.0
25	3871.5
26	4382.0
27	4985.5
28	5589.0
29	6923.5
30	8258.0
31	8258.0
32	9647.5
33	11037.0
34	12137.5
35	13238.0
36	14928.0
37	16618.0
38	18626.0
39	20634.0
40	23956.5
41	27279.0
42	32461.5
43	37644.0
44	37644.0
45	43883.5
46	50123.0
47	59638.5
48	69154.0
49	70237.5
50	71321.0
51	65818.0
52	60315.0
53	53009.0
54	45703.0
55	42377.5
56	39052.0
57	39052.0
58	36679.5
59	34307.0
60	32579.0
61	30851.0
62	28246.0
63	25641.0
64	22738.0
65	19835.0
66	17541.0
67	15247.0
68	13157.5
69	11068.0
70	11068.0
71	9609.5
72	8151.0
73	6766.0
74	5381.0
75	4595.0
76	3809.0
77	3112.5
78	2416.0
79	1917.5
80	1419.0
81	1419.0
82	1010.5
83	602.0
84	405.0
85	208.0
86	166.0
87	124.0
88	86.0
89	48.0
90	37.5
91	27.0
92	24.5
93	22.0
94	22.0
95	13.5
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	679870.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.037254350390526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.60147114358232	33.733184683640346
2	8.742112193865664	7.699572364818256
3	3.3184721060165026	4.3840920056197445
4	1.9574016814360242	3.4479438284512116
5	1.4028683980015084	3.088923623145866
6	1.1194692114768563	2.9579010241942467
7	0.9490012352903411	2.9253986144120887
8	0.8282002921371965	2.917733353433077
9	0.7256831899563227	2.8761385692518413
>10	4.267753984267353	28.69946202150302
>50	0.05273895704570622	1.5594203467662078
>100	0.029188851517400777	2.3822201921506263
>500	0.00298522345064264	0.802058438598171
>1k	0.0023218404616109425	1.740690295533492
>5k	3.316914945158489E-4	0.7852606384818371
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5376	0.7907394060629238	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2816	0.4141968317472458	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCG	2414	0.355067880624237	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	1520	0.2235721534999338	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTA	1388	0.20415667701178167	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	1383	0.20342124229632136	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCG	1272	0.18709459161310252	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTT	1124	0.16532572403547735	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTC	985	0.1448806389456808	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1890067218732993	0.0
2	0.0	0.0	0.0	0.7870622324856222	0.0
3	0.0	0.0	0.0	1.181843587744716	0.0
4	0.0	0.0	0.0	1.646785414858723	0.0
5	0.0	0.0	0.0	2.9107035168488093	0.0
6	0.0	0.0	0.0	4.4908585464868285	0.0
7	0.0	0.0	0.0	5.487078412049362	0.0
8	0.0	0.0	0.0	7.337285069204407	0.0
9	0.0	0.0	0.0	8.18582964390251	0.0
10	0.0	0.0	0.0	9.692147028108314	0.0
11	0.0	0.0	0.0	11.038139644343772	0.0
12	0.0	0.0	0.0	12.30573491991116	0.0
13	0.0	0.0	0.0	12.86731286863665	0.0
14	0.0	0.0	0.0	13.12545045376322	0.0
15	0.0	0.0	0.0	13.471104770029564	0.0
16	0.0	0.0	0.0	14.179769661847118	0.0
17	0.0	0.0	0.0	14.985953196934709	0.0
18	0.0	0.0	0.0	15.973642019797902	0.0
19	0.0	0.0	0.0	16.431670760586584	0.0
20	0.0	0.0	0.0	16.90808536926177	0.0
21	0.0	0.0	0.0	17.437010016620825	0.0
22	0.0	0.0	0.0	17.99476370482592	0.0
23	0.0	0.0	0.0	18.570020739258975	0.0
24	0.0	0.0	0.0	19.033050436112788	0.0
25	0.0	0.0	0.0	19.460779266624503	0.0
26	1.4708694309206171E-4	0.0	0.0	19.84011649285893	0.0
27	1.4708694309206171E-4	0.0	0.0	20.265786106167354	0.0
28	1.4708694309206171E-4	0.0	0.0	20.685866415638284	0.0
29	1.4708694309206171E-4	0.0	0.0	21.119919984702957	0.0
30	1.4708694309206171E-4	0.0	0.0	21.678850368452792	0.0
31	1.4708694309206171E-4	0.0	0.0	22.100842808183916	0.0
32	1.4708694309206171E-4	0.0	0.0	22.541515289687734	0.0
33	1.4708694309206171E-4	0.0	0.0	22.980422727874448	0.0
34	1.4708694309206171E-4	0.0	0.0	23.399914689573006	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATG	35	3.0539195E-7	40.0	1
GCGAAAA	25	7.79129E-5	40.0	9
ATATGCG	20	0.0012554069	40.0	1
GTTAACG	20	0.0012554069	40.0	1
CCATCCG	20	0.0012554069	40.0	1
CACTACG	25	7.79129E-5	40.0	1
AATTCGG	25	7.79129E-5	40.0	2
CCACGTC	35	3.0539195E-7	40.0	37
TAGTCCG	40	1.9263098E-8	40.0	1
CGTTTTT	2230	0.0	37.309418	1
AACACGC	65	0.0	36.923077	23
CTACGCG	55	2.1645974E-10	36.363636	1
ACACGAC	260	0.0	36.153843	26
ACAACGG	50	3.4197E-9	36.0	2
TATCGAG	50	3.4197E-9	36.0	1
GTCAAGC	270	0.0	35.555557	16
GCCGATA	45	5.4369593E-8	35.555553	9
TCGTTTA	40	8.6929685E-7	35.0	38
CGCATCG	40	8.6929685E-7	35.0	21
TTCGTCG	40	8.6929685E-7	35.0	28
>>END_MODULE