FastQCFastQC Report
Sat 18 Jun 2016
SRR3553728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362815
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112090.8224887457211727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28630.2100798714425656No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT17410.12775028158627547No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG15770.11571636649141666No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT15580.11432219340115862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG353.056939E-740.02
CCGTTAG200.001255850740.01
CGTTTTT50100.037.5648731
CGACGGT650.036.92307728
CACTTAC13150.035.74144737
CGACGTT408.7014996E-735.027
TATAACG408.7014996E-735.01
GCACTTA13550.034.9815536
GACCGAT7550.034.701999
ACTTACT13500.034.5185238
TATAGCG351.4012581E-534.2857131
TCGTGTA2350.034.04255317
CGTAAGG2600.033.8461532
CACGACC11300.033.8053127
CCTCGTG2500.033.615
TACGGGA4350.033.563224
TACGCGG1550.033.548392
ACGACCA11400.033.50877428
AACGAGC6400.033.437515
GCAACCG605.511538E-1033.3333361
Produced by FastQC (version 0.11.5)