##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553726_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669323
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.564063987043625	31.0	31.0	34.0	30.0	34.0
2	31.719589794463957	31.0	31.0	34.0	30.0	34.0
3	31.67140080349846	31.0	31.0	34.0	30.0	34.0
4	35.475737424233145	37.0	35.0	37.0	33.0	37.0
5	33.63759350866473	37.0	35.0	37.0	30.0	37.0
6	34.43383687696374	37.0	35.0	37.0	30.0	37.0
7	35.40380205072887	37.0	35.0	37.0	32.0	37.0
8	35.47203368179489	37.0	35.0	37.0	33.0	37.0
9	37.26666945555434	39.0	37.0	39.0	34.0	39.0
10	36.55499512193664	38.0	35.0	39.0	32.0	39.0
11	36.27869952175556	38.0	35.0	39.0	32.0	39.0
12	36.45940300871179	38.0	35.0	39.0	32.0	39.0
13	33.11163518958709	38.0	35.0	39.0	16.0	39.0
14	35.2506069565815	39.0	34.0	40.0	18.0	41.0
15	36.52345130826223	39.0	35.0	40.0	30.0	41.0
16	37.130440758796574	39.0	36.0	40.0	32.0	41.0
17	37.29940701275767	39.0	36.0	40.0	32.0	41.0
18	37.408406703489945	39.0	36.0	40.0	32.0	41.0
19	37.402266170443866	39.0	36.0	40.0	32.0	41.0
20	37.387085159183236	39.0	36.0	40.0	32.0	41.0
21	37.303795028708116	39.0	36.0	40.0	32.0	41.0
22	37.323398418999496	39.0	36.0	40.0	32.0	41.0
23	37.19974362153997	39.0	35.0	40.0	32.0	41.0
24	37.18584450257947	39.0	35.0	40.0	32.0	41.0
25	36.97522272505203	39.0	35.0	40.0	31.0	41.0
26	36.93107214304603	39.0	35.0	40.0	31.0	41.0
27	36.57651836258428	39.0	35.0	40.0	31.0	41.0
28	36.565159123472526	39.0	35.0	40.0	30.0	41.0
29	36.66508397290994	39.0	35.0	40.0	30.0	41.0
30	36.24301122178679	38.0	35.0	40.0	30.0	41.0
31	36.23997233025012	38.0	35.0	40.0	30.0	41.0
32	36.1520984636715	38.0	35.0	40.0	30.0	41.0
33	36.14535582969658	38.0	35.0	40.0	30.0	41.0
34	36.10219580083159	38.0	35.0	40.0	30.0	41.0
35	36.06593378682639	38.0	35.0	40.0	30.0	41.0
36	35.89540475973484	38.0	35.0	40.0	29.0	41.0
37	35.80690638152282	38.0	35.0	40.0	29.0	41.0
38	35.67308160633954	38.0	34.0	40.0	28.0	41.0
39	35.672463070894025	38.0	34.0	40.0	28.0	41.0
40	35.577199648002534	38.0	34.0	40.0	28.0	41.0
41	35.385816713305836	38.0	34.0	40.0	27.0	41.0
42	35.29260909904485	38.0	34.0	40.0	27.0	41.0
43	35.15303074898068	38.0	34.0	40.0	26.0	41.0
44	35.0476436638215	38.0	34.0	40.0	26.0	41.0
45	34.99415528825395	37.0	34.0	40.0	26.0	41.0
46	33.62045529587359	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	11.0
11	23.0
12	11.0
13	7.0
14	12.0
15	28.0
16	41.0
17	95.0
18	181.0
19	357.0
20	608.0
21	952.0
22	1620.0
23	2320.0
24	3331.0
25	4450.0
26	6316.0
27	8120.0
28	10631.0
29	13521.0
30	16987.0
31	22257.0
32	28498.0
33	38324.0
34	52976.0
35	66019.0
36	75729.0
37	99017.0
38	120906.0
39	95967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.48514394395531	22.870123990958028	22.057511844057352	20.58722022102931
2	25.226534871803302	28.023390799359948	23.027895350974045	23.722178977862708
3	28.296651990145268	27.215858412156763	22.982476323090644	21.505013274607325
4	25.18529917543548	27.99829081026649	22.780929386858066	24.035480627439966
5	20.64593626694436	35.22932873963692	19.840047331408005	24.284687662010718
6	19.085852421028413	40.92493459809389	20.801317151808618	19.187895829069074
7	81.79085434087877	5.500035110103791	8.192008940377066	4.517101608640372
8	81.42212354872012	5.620305891176606	7.499518169852223	5.458052390251044
9	75.40694104341253	6.810164897964062	10.674816194871536	7.108077863751881
10	35.33152155237456	26.328245107369685	18.998002459201313	19.34223088105444
11	28.584853650629068	27.580555277496817	22.357665880299944	21.47692519157417
12	23.50658800011355	24.334738235500648	30.73941878584779	21.419254978538017
13	20.631742820730796	31.446103002586195	29.012151084005776	18.910003092677226
14	21.667714989623843	29.138248648261005	27.84455337706907	21.349482985046084
15	19.22061545770876	27.288319690194417	32.3413359469195	21.14972890517732
16	20.683586250584547	25.289583653930915	30.46839866551725	23.558431429967293
17	20.712122547708656	25.728385248975457	27.597288603559118	25.962203599756766
18	21.39295975186868	25.574647815778036	29.273011684941352	23.75938074741194
19	23.14428160992525	27.322981579894908	26.672324124525826	22.860412685654012
20	24.904119535709963	25.564488296383065	26.591197374063047	22.94019479384393
21	24.710640453114564	27.774930788274123	27.187919733820593	20.32650902479072
22	22.315533755750213	24.89664930086072	25.87958280232414	26.90823414106493
23	21.268206829886317	27.88922538146754	26.489004561325398	24.35356322732074
24	21.890029178737322	25.92903575702613	28.44217216500852	23.738762899228025
25	22.611205651083257	27.685287970083206	25.932322660359798	23.77118371847374
26	21.813982187972027	26.98413172713324	26.663808056797688	24.538078028097047
27	23.26096667827043	26.614653911489672	26.353195691766157	23.77118371847374
28	20.688815414979018	26.666796150737387	29.636812122099492	23.007576312184103
29	23.084818540525276	25.702837045791043	26.99100434319454	24.221340070489138
30	24.5721422990096	25.696860857911652	27.251267325342177	22.47972951773658
31	26.03048154627885	25.501290109558468	25.175587870131462	23.292640474031224
32	24.87842192782857	26.10114996795269	27.288767904285375	21.731660199933366
33	23.585174870727585	23.846782495148084	26.899718073336786	25.668324560787543
34	24.032940747591223	23.811522986659657	28.840036873079217	23.315499392669906
35	24.69764224447688	22.538445563651628	28.356264464242226	24.40764772762926
36	25.64770671260363	24.396591780052383	27.682299876143507	22.273401631200482
37	23.688712325738095	25.387443730456	28.664635758818985	22.25920818498692
38	22.010299959810137	25.27449377953544	29.743636480443676	22.97156978021075
39	23.6807938767979	23.37152615403923	28.116171116187548	24.831508852975322
40	25.15422299846263	22.1676231057352	30.989821057994423	21.688332837807756
41	22.36887123257381	23.953158639401305	27.92493310404692	25.75303702397796
42	23.606689147093405	23.677357568767246	28.87514697687066	23.84080630726869
43	23.09198996598055	22.565338409108907	28.913992198086724	25.42867942682382
44	23.28606666736389	23.333278551611105	29.15647602129316	24.22417875973185
45	23.66420995543258	22.21095046786081	28.70258455185314	25.422255024853474
46	25.500991300164493	22.85727518701733	28.471604890314538	23.170128622503633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3532.0
1	3054.0
2	2576.0
3	3167.5
4	3759.0
5	2781.0
6	1803.0
7	1803.0
8	1742.0
9	1681.0
10	1767.5
11	1854.0
12	1799.5
13	1745.0
14	1773.5
15	1802.0
16	1670.5
17	1539.0
18	1542.5
19	1546.0
20	1546.0
21	1692.0
22	1838.0
23	2151.0
24	2464.0
25	3255.0
26	4046.0
27	4359.5
28	4673.0
29	5901.5
30	7130.0
31	7130.0
32	8379.5
33	9629.0
34	11358.0
35	13087.0
36	14765.5
37	16444.0
38	19075.5
39	21707.0
40	25293.5
41	28880.0
42	34999.5
43	41119.0
44	41119.0
45	47150.5
46	53182.0
47	61865.0
48	70548.0
49	72448.0
50	74348.0
51	68840.0
52	63332.0
53	55676.5
54	48021.0
55	43977.0
56	39933.0
57	39933.0
58	36611.0
59	33289.0
60	31288.5
61	29288.0
62	26982.0
63	24676.0
64	21607.0
65	18538.0
66	15892.0
67	13246.0
68	11336.5
69	9427.0
70	9427.0
71	7956.0
72	6485.0
73	5597.5
74	4710.0
75	4015.5
76	3321.0
77	2727.0
78	2133.0
79	1594.0
80	1055.0
81	1055.0
82	785.0
83	515.0
84	379.5
85	244.0
86	168.0
87	92.0
88	75.5
89	59.0
90	37.0
91	15.0
92	11.5
93	8.0
94	8.0
95	6.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	669323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.465372435508336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25206338284114	28.568119536141307
2	9.76399014229926	7.316230542757475
3	3.647604038419007	4.0997653138989705
4	1.9057921310584536	2.856048478990643
5	1.1596710600187152	2.1723754083140956
6	0.7951172007500648	1.7873617233588015
7	0.5721999085598178	1.5006377877230173
8	0.446132783640729	1.337162471583198
9	0.39013634822549104	1.3154943228207472
>10	4.869879622097686	39.98886799625996
>50	0.1563513987448339	3.647345244664869
>100	0.034349928375000664	2.3127810645342044
>500	0.0035534408663793794	0.7529296845845284
>1k	0.0031586141034483373	2.344880424368162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3138	0.4688319391385027	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	2148	0.32092128912348744	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	2092	0.3125546260923351	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCG	1998	0.2985105845757579	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	1965	0.29358022957525737	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTA	1710	0.2554820318441171	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1556	0.2324737085084481	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT	1245	0.18600884774615545	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22918680517478107	0.0
2	0.0	0.0	0.0	1.1150072535980387	0.0
3	0.0	0.0	0.0	1.4478809184803152	0.0
4	0.0	0.0	0.0	1.8971408423138008	0.0
5	0.0	0.0	0.0	3.2178783636599966	0.0
6	0.0	0.0	0.0	4.376063574686661	0.0
7	0.0	0.0	0.0	5.21676380462049	0.0
8	0.0	0.0	0.0	6.694824471891748	0.0
9	0.0	0.0	0.0	7.274365291496034	0.0
10	0.0	0.0	0.0	8.685791463912043	0.0
11	0.0	0.0	0.0	10.08705811693308	0.0
12	0.0	0.0	0.0	11.555556883597307	0.0
13	0.0	0.0	0.0	12.075784038498602	0.0
14	0.0	0.0	0.0	12.358756534587934	0.0
15	0.0	0.0	0.0	12.744668866899838	0.0
16	0.0	0.0	0.0	13.580139932439195	0.0
17	0.0	0.0	0.0	14.540065110566946	0.0
18	0.0	0.0	0.0	15.644315225982075	0.0
19	0.0	0.0	0.0	16.175150114369295	0.0
20	0.0	0.0	0.0	16.679839180784164	0.0
21	0.0	0.0	0.0	17.30883295509044	0.0
22	0.0	0.0	0.0	17.94499815485199	0.0
23	0.0	0.0	0.0	18.60267763097936	0.0
24	0.0	0.0	0.0	19.15487739103542	0.0
25	0.0	0.0	0.0	19.61041231214227	0.0
26	0.0	0.0	0.0	20.032331176427526	0.0
27	0.0	0.0	0.0	20.48218871904895	0.0
28	0.0	0.0	0.0	20.939665901216603	0.0
29	0.0	0.0	0.0	21.432103782478713	0.0
30	0.0	0.0	0.0	21.997600560566422	0.0
31	4.482140909545914E-4	0.0	0.0	22.483614039858185	0.0
32	4.482140909545914E-4	0.0	0.0	22.949159075663022	0.0
33	4.482140909545914E-4	0.0	0.0	23.43323029389398	0.0
34	4.482140909545914E-4	0.0	0.0	23.901165804850574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGACA	25	7.791159E-5	40.000004	18
TGCGTAG	25	7.791159E-5	40.000004	1
TACCGAC	25	7.791159E-5	40.000004	4
CCTTACG	20	0.0012553931	40.0	17
GCGAAAC	20	0.0012553931	40.0	23
GCGTTAG	30	4.864465E-6	40.0	1
CGAATGT	20	0.0012553931	40.0	23
TCGTACA	30	4.864465E-6	40.0	34
TCGACGT	30	4.864465E-6	40.0	26
TATCGCG	30	4.864465E-6	40.0	1
TACGATT	35	3.0538286E-7	39.999996	11
GTACGAT	35	3.0538286E-7	39.999996	10
GCGATGT	95	0.0	37.894737	9
CTACGCA	130	0.0	36.923077	15
CGCTACA	120	0.0	36.666664	28
TATAGCG	60	1.4551915E-11	36.666664	1
TACACGA	230	0.0	36.52174	9
GCGGGAT	225	0.0	36.444443	5
CACAAAT	165	0.0	36.36364	32
CTATGCG	55	2.1645974E-10	36.363636	1
>>END_MODULE