##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	881783
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.643721868078654	31.0	31.0	34.0	30.0	34.0
2	31.802674807747483	31.0	31.0	34.0	30.0	34.0
3	31.730056034194355	31.0	31.0	34.0	30.0	34.0
4	35.53041507944698	37.0	35.0	37.0	33.0	37.0
5	33.634166229106256	37.0	35.0	37.0	30.0	37.0
6	34.47497626967179	37.0	35.0	37.0	30.0	37.0
7	35.51559510673261	37.0	35.0	37.0	32.0	37.0
8	35.63844052334871	37.0	35.0	37.0	35.0	37.0
9	37.43096203941332	39.0	37.0	39.0	35.0	39.0
10	36.62898127997478	38.0	35.0	39.0	32.0	39.0
11	36.35010427735622	38.0	35.0	39.0	32.0	39.0
12	36.65673187167364	39.0	35.0	39.0	32.0	39.0
13	32.97200785227204	38.0	35.0	39.0	9.0	39.0
14	35.2640978562753	39.0	35.0	40.0	17.0	41.0
15	36.685105065532	39.0	35.0	41.0	30.0	41.0
16	37.325882898626986	39.0	36.0	41.0	32.0	41.0
17	37.52523693471069	39.0	36.0	40.0	32.0	41.0
18	37.61040641518378	39.0	36.0	40.0	33.0	41.0
19	37.62422500774	39.0	36.0	41.0	32.0	41.0
20	37.62332796164136	39.0	36.0	41.0	33.0	41.0
21	37.56228913462836	39.0	36.0	41.0	32.0	41.0
22	37.57700817548081	39.0	36.0	41.0	33.0	41.0
23	37.47042639742431	39.0	36.0	40.0	32.0	41.0
24	37.46348251213734	39.0	36.0	41.0	32.0	41.0
25	37.269245381233254	39.0	36.0	40.0	32.0	41.0
26	37.23736792385428	39.0	36.0	40.0	32.0	41.0
27	36.85785164830803	39.0	35.0	40.0	31.0	41.0
28	36.94774904936929	39.0	35.0	40.0	31.0	41.0
29	37.042605720455036	39.0	35.0	40.0	31.0	41.0
30	36.584977256309095	39.0	35.0	40.0	31.0	41.0
31	36.70399973689672	39.0	35.0	40.0	30.0	41.0
32	36.67185804217137	39.0	35.0	40.0	30.0	41.0
33	36.63964263316485	39.0	35.0	40.0	30.0	41.0
34	36.63163159189959	39.0	35.0	40.0	30.0	41.0
35	36.56928632101095	39.0	35.0	40.0	30.0	41.0
36	36.4845092273269	39.0	35.0	40.0	30.0	41.0
37	36.478601878239886	39.0	35.0	40.0	30.0	41.0
38	36.40603073545305	39.0	35.0	40.0	30.0	41.0
39	36.38660305313212	39.0	35.0	40.0	30.0	41.0
40	36.280122206937534	39.0	35.0	40.0	30.0	41.0
41	36.14667327449044	39.0	35.0	40.0	30.0	41.0
42	36.07202792523784	38.0	35.0	40.0	29.0	41.0
43	36.00162284825178	38.0	35.0	40.0	29.0	41.0
44	35.97381782139143	38.0	35.0	40.0	29.0	41.0
45	35.93904622792682	38.0	35.0	40.0	29.0	41.0
46	34.777837631253945	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	25.0
11	17.0
12	7.0
13	8.0
14	11.0
15	18.0
16	35.0
17	72.0
18	145.0
19	286.0
20	553.0
21	966.0
22	1535.0
23	2364.0
24	3449.0
25	5066.0
26	6634.0
27	9385.0
28	12232.0
29	15207.0
30	19295.0
31	25065.0
32	33213.0
33	44975.0
34	62983.0
35	80588.0
36	97671.0
37	134048.0
38	171872.0
39	154042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.50336760858397	23.107045610994994	22.348242141207077	21.041344639213957
2	26.1018867453784	27.23413810427282	24.04106225681375	22.62291289353503
3	27.53035610802204	27.461971936406123	24.132127745715216	20.87554420985662
4	24.035278520905937	28.31649056513904	23.794629744506302	23.85360116944872
5	21.01560134409486	35.19346596611638	20.92351519591555	22.86741749387321
6	19.77243834367412	40.23983224897736	21.809220635916095	18.178508771432426
7	84.32267349223108	4.564841916888849	7.46351426598154	3.6489703248985297
8	84.33923085384953	4.307749185457193	7.083148575102944	4.269871385590332
9	78.0709086022298	6.841479139425459	9.71769698440546	5.36991527393928
10	31.572847287824786	34.25139745266125	17.516894746213072	16.65886051330089
11	24.161953677945707	27.486240945901656	29.521889172279348	18.82991620387329
12	22.263527421145564	24.86280638206906	32.14917956005049	20.724486636734888
13	20.21926029419937	32.348888558749714	30.75541261285373	16.67643853419719
14	20.300119190322334	29.49478499812312	29.992753319127267	20.212342492427275
15	19.522376820600986	28.44407297487023	33.27179135909855	18.761758845430222
16	21.235950341523935	27.67449587937168	31.03620732084878	20.053346458255604
17	21.634234273058112	27.081833058700383	29.609665870174407	21.674266798067098
18	21.98534106463835	25.934385217224648	30.823910191056076	21.256363527080925
19	22.23642324698934	28.230755185799683	29.375481269201153	20.157340298009828
20	24.730914522053613	26.468530239299238	29.093098868996115	19.707456369651037
21	24.097992363200472	28.026056297297636	29.718649599731457	18.157301739770443
22	22.653192452111234	24.965212529613297	29.827519922702074	22.5540750955734
23	20.60370862218936	28.180062441666486	29.567932246369004	21.648296689775147
24	20.813624213667083	26.109484986669056	31.300104447466097	21.776786352197764
25	20.39900973368731	29.474144999393275	28.57982065882422	21.54702460809519
26	20.220847986409353	29.553643016479107	29.014281291428844	21.211227705682692
27	20.835398278261206	28.74562108818156	29.045014476350755	21.37396615720648
28	19.468508692047816	28.020839594321963	30.68113129874357	21.829520414886655
29	20.912174537272776	27.075595696446857	29.395440828412433	22.616788937867934
30	21.28902462397211	27.471044463320343	29.939452223506237	21.30047868920131
31	22.58004520386535	27.44870336579408	28.328511663300382	21.64273976704019
32	22.137305890451504	27.645917419591896	28.510642641103313	21.70613404885329
33	21.501208347178387	26.216087177911117	29.50465137114233	22.778053103768162
34	21.728248333206697	26.588401001153343	29.97630936409525	21.70704130154471
35	21.041117826041102	26.35648453190864	29.87503728241529	22.727360359634968
36	22.708534866287962	27.3882576552281	29.81379772574432	20.08940975273962
37	22.63935684856705	26.56719396949136	29.76820827800037	21.02524090394122
38	21.477052744269283	26.764067803529894	29.993433758645832	21.76544569355499
39	23.30539373065709	25.051741755057648	29.10330546177461	22.53955905251065
40	22.85573661547115	25.381414701803052	31.023505783168876	20.73934289955692
41	21.434752087531738	26.471592217132788	30.132243420433376	21.961412274902102
42	22.667141462241844	26.439271340000886	29.75856871815401	21.13501847960326
43	22.670770473007533	25.298514487124386	29.909399478102888	22.121315561765194
44	22.050549851834294	25.900703461055613	28.986156457994767	23.062590229115326
45	21.83575777714018	25.2131193275443	29.321839953820838	23.629282941494676
46	22.609077289990847	25.7681311615216	29.576437740351086	22.04635380813647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3157.0
1	2756.5
2	2356.0
3	4906.5
4	7457.0
5	5092.5
6	2728.0
7	2728.0
8	2570.5
9	2413.0
10	2443.5
11	2474.0
12	2522.5
13	2571.0
14	2501.5
15	2432.0
16	2462.5
17	2493.0
18	2521.0
19	2549.0
20	2549.0
21	3251.0
22	3953.0
23	4409.0
24	4865.0
25	6498.5
26	8132.0
27	9937.5
28	11743.0
29	13958.5
30	16174.0
31	16174.0
32	18727.0
33	21280.0
34	24358.5
35	27437.0
36	30470.0
37	33503.0
38	38079.5
39	42656.0
40	46782.0
41	50908.0
42	57326.5
43	63745.0
44	63745.0
45	70829.0
46	77913.0
47	86813.5
48	95714.0
49	94193.0
50	92672.0
51	84747.5
52	76823.0
53	68095.5
54	59368.0
55	52627.5
56	45887.0
57	45887.0
58	40972.0
59	36057.0
60	31998.0
61	27939.0
62	23794.5
63	19650.0
64	16290.5
65	12931.0
66	10595.5
67	8260.0
68	7020.0
69	5780.0
70	5780.0
71	4510.0
72	3240.0
73	2536.0
74	1832.0
75	1430.5
76	1029.0
77	929.0
78	829.0
79	651.0
80	473.0
81	473.0
82	361.0
83	249.0
84	151.5
85	54.0
86	45.0
87	36.0
88	24.0
89	12.0
90	10.5
91	9.0
92	4.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	881783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.09550461140283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.80664817203655	30.79601315968286
2	9.18517890932132	7.3656876663050594
3	3.3979450399634277	4.0872696305744105
4	1.866561246278222	2.993628602304571
5	1.1741209617770298	2.3538486218637815
6	0.8084325344341426	1.9448706247447347
7	0.647165347722743	1.8163894828770153
8	0.5412162182974858	1.7360269901210277
9	0.445338798344418	1.6070475458389688
>10	5.036488587731647	39.70737764990167
>50	0.06528588201714926	1.712196935801433
>100	0.0209353866428555	1.5382277889672018
>500	0.0024791905233573666	0.5920083972570703
>1k	0.0022037249096509928	1.749406903760212
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3484	0.39510854711419924	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2746	0.3114144863305371	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG	2099	0.23804042491179803	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT	1692	0.1918839442357133	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT	1678	0.19029625202572514	No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT	1447	0.16409933056092033	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCG	1360	0.1542329575417081	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTA	1333	0.1511709797081595	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.186213614914327	0.0
2	0.0	0.0	0.0	0.7523392943615379	0.0
3	0.0	0.0	0.0	1.0222469700595271	0.0
4	0.0	0.0	0.0	1.4180359566922929	0.0
5	0.0	0.0	0.0	2.4021783137121036	0.0
6	0.0	0.0	0.0	3.3627321007549478	0.0
7	0.0	0.0	0.0	4.041130300765608	0.0
8	0.0	0.0	0.0	5.213981217601156	0.0
9	0.0	0.0	0.0	5.703557451209651	0.0
10	0.0	0.0	0.0	6.756991232536803	0.0
11	0.0	0.0	0.0	7.895820173444033	0.0
12	0.0	0.0	0.0	8.995410435447269	0.0
13	1.1340658642772655E-4	0.0	0.0	9.436562056651127	0.0
14	1.1340658642772655E-4	0.0	0.0	9.69478885394706	0.0
15	1.1340658642772655E-4	0.0	0.0	10.021173009686057	0.0
16	1.1340658642772655E-4	0.0	0.0	10.720891647945129	0.0
17	1.1340658642772655E-4	0.0	0.0	11.561461266547438	0.0
18	1.1340658642772655E-4	0.0	0.0	12.450682310727242	0.0
19	1.1340658642772655E-4	0.0	0.0	12.95216623591065	0.0
20	1.1340658642772655E-4	0.0	0.0	13.42654598693783	0.0
21	1.1340658642772655E-4	0.0	0.0	14.034745509949726	0.0
22	1.1340658642772655E-4	0.0	0.0	14.65814151554294	0.0
23	1.1340658642772655E-4	0.0	0.0	15.317374002447314	0.0
24	1.1340658642772655E-4	0.0	0.0	15.813527818068618	0.0
25	1.1340658642772655E-4	0.0	0.0	16.278608229008725	0.0
26	3.4021975928317963E-4	0.0	0.0	16.690047324568518	0.0
27	3.4021975928317963E-4	0.0	0.0	17.139137406822314	0.0
28	3.4021975928317963E-4	0.0	0.0	17.595939136953195	0.0
29	3.4021975928317963E-4	0.0	0.0	18.094361084303053	0.0
30	3.4021975928317963E-4	0.0	0.0	18.671373796047327	0.0
31	3.4021975928317963E-4	0.0	0.0	19.184311786459933	0.0
32	3.4021975928317963E-4	0.0	0.0	19.66991878954346	0.0
33	3.4021975928317963E-4	0.0	0.0	20.15076271599702	0.0
34	3.4021975928317963E-4	0.0	0.0	20.646349498686185	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	45	1.2205419E-9	40.0	1
AACGTCG	20	0.0012556099	40.0	1
CTATGCG	45	1.2205419E-9	40.0	1
CGGCGAT	40	1.9274012E-8	40.0	6
CTATACG	35	3.055302E-7	40.0	1
CCGGATG	25	7.7931756E-5	40.0	1
ATATGCG	20	0.0012556099	40.0	1
CTACGTA	20	0.0012556099	40.0	21
CATAGCG	45	1.2205419E-9	40.0	1
ACGCTAC	25	7.7931756E-5	40.0	21
AATAGCG	40	1.9274012E-8	40.0	1
TTCGCGG	85	0.0	40.0	2
CCACGCG	20	0.0012556099	40.0	1
TCGACAA	25	7.7931756E-5	40.0	19
CGTCGGG	70	0.0	37.142857	3
CGCACGG	140	0.0	37.142857	2
TTAACGG	65	0.0	36.923077	2
CGTTTTT	1870	0.0	36.791443	1
ACGCATT	115	0.0	36.52174	17
GCGATCG	55	2.1645974E-10	36.363636	9
>>END_MODULE