FastQCFastQC Report
Sat 18 Jun 2016
SRR3553723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917219
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37000.40339330083655045No Hit
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG24990.27245401588933504No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT23480.2559912082065461No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT21510.23451324056741082No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21290.23211468580568No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTA19540.2130352729282756No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCG19340.21085476859942937No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT18930.20638473472529462No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9510.10368298083663771No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA9430.10281077910509921No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG555.456968E-1240.01
CCTTACG200.001255636340.01
CTCCGTA200.001255636340.014
CTATCGG451.2205419E-940.02
GCTATCG200.001255636340.01
CGTTCCG257.793421E-540.02
TCTAGCG555.456968E-1240.01
TAGCACG200.001255636340.01
TCTACGG800.040.02
CGTAGAC200.001255636340.030
TCGTAGA200.001255636340.029
TATCGAT257.793421E-540.032
ATTTCGA257.793421E-540.034
ACTACGG1100.038.1818162
CGGGCTA850.037.6470576
CGCAAGG3150.036.8253972
GCGATAT1200.036.6666689
ACGGGAA4950.036.3636365
AATTGCG552.1645974E-1036.3636361
ACGCATT1600.036.2517