##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553723_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 917219 Sequences flagged as poor quality 0 Sequence length 46 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.628277434287778 31.0 31.0 34.0 30.0 34.0 2 31.785303182773145 31.0 31.0 34.0 30.0 34.0 3 31.741578619718954 31.0 31.0 34.0 30.0 34.0 4 35.53016564201134 37.0 35.0 37.0 33.0 37.0 5 33.58540653867833 37.0 35.0 37.0 30.0 37.0 6 34.436141205099325 37.0 35.0 37.0 30.0 37.0 7 35.474933467361666 37.0 35.0 37.0 32.0 37.0 8 35.587113873567816 37.0 35.0 37.0 35.0 37.0 9 37.3973271377937 39.0 37.0 39.0 35.0 39.0 10 36.589989958777565 38.0 35.0 39.0 32.0 39.0 11 36.33099946686669 38.0 35.0 39.0 32.0 39.0 12 36.52661141995532 38.0 35.0 39.0 32.0 39.0 13 32.71028293133919 38.0 34.0 39.0 0.0 39.0 14 35.00132465637977 39.0 34.0 40.0 17.0 41.0 15 36.458679988094445 39.0 34.0 40.0 30.0 41.0 16 37.15078732560054 39.0 36.0 40.0 32.0 41.0 17 37.347940895249664 39.0 36.0 40.0 32.0 41.0 18 37.46965991764235 39.0 36.0 40.0 32.0 41.0 19 37.48031277154093 39.0 36.0 40.0 32.0 41.0 20 37.492863754457765 39.0 36.0 40.0 32.0 41.0 21 37.43512618033425 39.0 36.0 40.0 32.0 41.0 22 37.45484339072784 39.0 36.0 40.0 32.0 41.0 23 37.326689700060726 39.0 36.0 40.0 32.0 41.0 24 37.29801061687558 39.0 35.0 40.0 32.0 41.0 25 37.095858241052575 39.0 35.0 40.0 31.0 41.0 26 37.05638893219613 39.0 35.0 40.0 31.0 41.0 27 36.630563693076574 39.0 35.0 40.0 31.0 41.0 28 36.703642205405686 39.0 35.0 40.0 31.0 41.0 29 36.796471725945494 39.0 35.0 40.0 31.0 41.0 30 36.28546726572389 39.0 35.0 40.0 30.0 41.0 31 36.390858671702176 39.0 35.0 40.0 30.0 41.0 32 36.352319347941986 38.0 35.0 40.0 30.0 41.0 33 36.34891994169332 38.0 35.0 40.0 30.0 41.0 34 36.28852433279293 38.0 35.0 40.0 30.0 41.0 35 36.29419582455226 39.0 35.0 40.0 30.0 41.0 36 36.19557270401071 38.0 35.0 40.0 30.0 41.0 37 36.218879024529585 38.0 35.0 40.0 30.0 41.0 38 36.115467516481885 38.0 35.0 40.0 30.0 41.0 39 36.08136006777008 38.0 35.0 40.0 30.0 41.0 40 35.98678723401935 38.0 35.0 40.0 29.0 41.0 41 35.86264131030866 38.0 35.0 40.0 29.0 41.0 42 35.85056458708335 38.0 35.0 40.0 29.0 41.0 43 35.782460895380495 38.0 35.0 40.0 29.0 41.0 44 35.707073228967126 38.0 35.0 40.0 29.0 41.0 45 35.63655789947657 38.0 34.0 40.0 28.0 41.0 46 34.462918888509726 37.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 14.0 10 23.0 11 20.0 12 17.0 13 12.0 14 12.0 15 29.0 16 42.0 17 107.0 18 192.0 19 391.0 20 657.0 21 1142.0 22 1842.0 23 2711.0 24 4050.0 25 5538.0 26 7652.0 27 10230.0 28 13220.0 29 16697.0 30 21186.0 31 28228.0 32 37433.0 33 50817.0 34 70919.0 35 89564.0 36 103237.0 37 137486.0 38 168734.0 39 145011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.57896205813443 22.945774128098087 21.7112816023218 19.763982211445686 2 25.157350643630366 27.23286368904264 23.699683499796667 23.91010216753033 3 27.892466248518623 27.278218179082643 23.214412261411944 21.6149033109868 4 24.677748716500638 28.449149003673057 22.781582152135968 24.091520127690334 5 21.109571432776686 35.84531066190299 19.773794480925496 23.271323424394826 6 18.841628880343738 40.20501101699812 21.960186171459597 18.993173931198548 7 83.89795675841866 4.804196162530432 7.410007860718106 3.887839218332808 8 83.52999665292586 4.67292980193389 6.969327935858284 4.827745609281971 9 77.76158147617963 6.4368487787540385 9.758411022885483 6.043158722180853 10 32.614348372635106 31.49487745020546 17.602775345909755 18.28799883124968 11 27.13670344814052 26.630281317765984 26.132908280356165 20.100106953737328 12 23.140493164664054 25.489550478130084 30.606976087499277 20.76298026970658 13 20.43416021691657 32.0876475520023 29.954460167092044 17.52373206398908 14 21.164193066214285 29.47954632426934 29.05587433317452 20.300386276341857 15 19.40332679545452 28.049353534979105 33.093514198899065 19.45380547066731 16 22.508255934515095 26.26482879225136 29.646354905426076 21.58056036780747 17 21.767429588789593 25.98779571727145 28.423201002159786 23.82157369177917 18 22.279957131284895 25.729187903870287 29.76955340000589 22.22130156483893 19 22.959729355802704 27.78671178856958 28.041612744611697 21.211946111016015 20 24.463078065325732 26.540008438551755 27.446117012403796 21.55079648371872 21 24.89340059462353 27.900098013669584 28.1217462786968 19.08475511301009 22 22.895295452885296 25.03077236734084 27.476316997358317 24.59761518241554 23 20.54165908032869 28.302073986692385 27.881127626008617 23.27513930697031 24 21.69329244160882 25.967844102662507 29.811201032686853 22.527662423041825 25 21.41440593794939 28.916867182210577 27.034655845550514 22.634071034289523 26 20.353045455883493 29.23511178900568 27.660787663578706 22.75105509153212 27 21.3008016624165 28.051206963658625 27.680739278187648 22.967252095737223 28 19.15791103324288 28.3464472497844 29.391235898951066 23.10440581802165 29 20.427291628280706 27.902060467565544 27.720424456972655 23.9502234471811 30 21.060728135810532 29.181035281650292 27.127545329959368 22.63069125257981 31 21.84941655155421 29.785471081606463 24.94900345500911 23.416108911830218 32 21.553958214995546 29.649734687135787 25.39764222066922 23.398664877199447 33 21.006215527589376 28.02558603779468 25.429477583870373 25.538720850745566 34 19.144173855971147 29.621388130860787 27.17137346696917 24.06306454619889 35 19.127492997855473 27.75760205577948 26.527579563877328 26.587325382487712 36 20.545147887254846 30.555298134905623 26.015161046598468 22.884392931241067 37 19.436034360387215 28.947176192381534 27.115661581367156 24.501127865864095 38 19.37072825573827 29.733029952497713 25.964573346169235 24.93166844559478 39 20.51244032232215 27.57400359129063 27.43390618816226 24.479649898224963 40 21.871221594842673 26.824128152600412 28.249959933232958 23.054690319323957 41 18.808703264978156 28.04815425759824 27.458545887078223 25.684596590345382 42 20.39392991204936 27.540750900275725 27.687934942472847 24.377384245202073 43 19.81794969358463 26.01003686142568 29.040610802872596 25.131402642117095 44 20.42652845176561 27.302966903215044 27.62535446823496 24.645150176784387 45 20.670199810514173 26.81824079091253 26.745302921112625 25.76625647746067 46 21.814855557941996 27.949050335852178 26.46816082091627 23.767933285289555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4237.0 1 3823.5 2 3410.0 3 4324.0 4 5238.0 5 3798.0 6 2358.0 7 2358.0 8 2311.5 9 2265.0 10 2276.5 11 2288.0 12 2309.0 13 2330.0 14 2206.0 15 2082.0 16 2159.5 17 2237.0 18 2358.0 19 2479.0 20 2479.0 21 2635.0 22 2791.0 23 3273.0 24 3755.0 25 4750.0 26 5745.0 27 7190.0 28 8635.0 29 10556.0 30 12477.0 31 12477.0 32 14981.0 33 17485.0 34 19785.0 35 22085.0 36 26154.0 37 30223.0 38 34391.5 39 38560.0 40 46748.0 41 54936.0 42 63725.0 43 72514.0 44 72514.0 45 84999.5 46 97485.0 47 98262.5 48 99040.0 49 93339.5 50 87639.0 51 81307.5 52 74976.0 53 67750.0 54 60524.0 55 55664.5 56 50805.0 57 50805.0 58 45482.5 59 40160.0 60 34953.5 61 29747.0 62 26692.0 63 23637.0 64 20881.5 65 18126.0 66 15727.5 67 13329.0 68 10636.0 69 7943.0 70 7943.0 71 6743.0 72 5543.0 73 4847.5 74 4152.0 75 3400.5 76 2649.0 77 2253.0 78 1857.0 79 1384.0 80 911.0 81 911.0 82 602.5 83 294.0 84 209.5 85 125.0 86 95.0 87 65.0 88 58.0 89 51.0 90 36.5 91 22.0 92 15.0 93 8.0 94 8.0 95 4.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 917219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.47410571587898 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52698190053357 27.14726246056685 2 9.718532619093704 6.895125070658965 3 3.728582852986588 3.968044268917796 4 1.9585201447110672 2.7790700264063593 5 1.1521913187387094 2.043647832292749 6 0.8172682382034415 1.7395115928155427 7 0.591782814281419 1.4695076280261605 8 0.43513595168817787 1.2348847000772822 9 0.36003544613620664 1.14947419299295 >10 4.167578872181405 35.51897990758881 >50 0.5035855360611969 11.131027858695699 >100 0.0352810888635739 2.3285528698194624 >500 0.0021108343764454896 0.6043882632512487 >1k 0.002412382144509131 1.9905233278901302 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3700 0.40339330083655045 No Hit GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG 2499 0.27245401588933504 No Hit GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT 2348 0.2559912082065461 No Hit CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT 2151 0.23451324056741082 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2129 0.23211468580568 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTA 1954 0.2130352729282756 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCG 1934 0.21085476859942937 No Hit CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT 1893 0.20638473472529462 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 951 0.10368298083663771 No Hit ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA 943 0.10281077910509921 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.2432352578827957 0.0 2 0.0 0.0 0.0 1.0030319912692607 0.0 3 0.0 0.0 0.0 1.2929300417893654 0.0 4 0.0 0.0 0.0 1.7246698989009168 0.0 5 0.0 0.0 0.0 2.875540083665951 0.0 6 0.0 0.0 0.0 3.8251497188784795 0.0 7 0.0 0.0 0.0 4.525418684087443 0.0 8 0.0 0.0 0.0 5.765144420252961 0.0 9 0.0 0.0 0.0 6.2094221772553775 0.0 10 0.0 0.0 0.0 7.375664917538778 0.0 11 0.0 0.0 0.0 8.593040484333622 0.0 12 0.0 0.0 0.0 9.812487530240869 0.0 13 0.0 0.0 0.0 10.254475757697998 0.0 14 1.0902521644231094E-4 0.0 0.0 10.51929800843637 0.0 15 1.0902521644231094E-4 0.0 0.0 10.864253793259843 0.0 16 1.0902521644231094E-4 0.0 0.0 11.631791317013713 0.0 17 1.0902521644231094E-4 0.0 0.0 12.532012529177873 0.0 18 1.0902521644231094E-4 0.0 0.0 13.54954487423396 0.0 19 1.0902521644231094E-4 0.0 0.0 14.111024738911864 0.0 20 1.0902521644231094E-4 0.0 0.0 14.629548668311493 0.0 21 1.0902521644231094E-4 0.0 0.0 15.29318516079584 0.0 22 1.0902521644231094E-4 0.0 0.0 15.989202142563553 0.0 23 2.1805043288462188E-4 0.0 0.0 16.692741864265788 0.0 24 2.1805043288462188E-4 0.0 0.0 17.229145929161955 0.0 25 2.1805043288462188E-4 0.0 0.0 17.7283723952513 0.0 26 2.1805043288462188E-4 0.0 0.0 18.174939681799003 0.0 27 2.1805043288462188E-4 0.0 0.0 18.64614666726267 0.0 28 2.1805043288462188E-4 0.0 0.0 19.138940645581915 0.0 29 2.1805043288462188E-4 0.0 0.0 19.67785229045626 0.0 30 2.1805043288462188E-4 0.0 0.0 20.282070039979548 0.0 31 2.1805043288462188E-4 0.0 0.0 20.819455331823697 0.0 32 3.2707564932693285E-4 0.0 0.0 21.327512840444868 0.0 33 3.2707564932693285E-4 0.0 0.0 21.83535229863315 0.0 34 3.2707564932693285E-4 0.0 0.0 22.36085384188509 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACG 55 5.456968E-12 40.0 1 CCTTACG 20 0.0012556363 40.0 1 CTCCGTA 20 0.0012556363 40.0 14 CTATCGG 45 1.2205419E-9 40.0 2 GCTATCG 20 0.0012556363 40.0 1 CGTTCCG 25 7.793421E-5 40.0 2 TCTAGCG 55 5.456968E-12 40.0 1 TAGCACG 20 0.0012556363 40.0 1 TCTACGG 80 0.0 40.0 2 CGTAGAC 20 0.0012556363 40.0 30 TCGTAGA 20 0.0012556363 40.0 29 TATCGAT 25 7.793421E-5 40.0 32 ATTTCGA 25 7.793421E-5 40.0 34 ACTACGG 110 0.0 38.181816 2 CGGGCTA 85 0.0 37.647057 6 CGCAAGG 315 0.0 36.825397 2 GCGATAT 120 0.0 36.666668 9 ACGGGAA 495 0.0 36.363636 5 AATTGCG 55 2.1645974E-10 36.363636 1 ACGCATT 160 0.0 36.25 17 >>END_MODULE