Basic Statistics
Measure | Value |
---|---|
Filename | SRR3553722_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 721912 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3110 | 0.43080042996930373 | No Hit |
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT | 1700 | 0.23548576557807602 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1695 | 0.2347931603851993 | No Hit |
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT | 1429 | 0.19794656412415917 | No Hit |
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT | 1269 | 0.17578319795210495 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG | 1229 | 0.1702423564090914 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCG | 970 | 0.13436540741807868 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTA | 899 | 0.12453041367922961 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 777 | 0.10763084697303826 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGATCG | 20 | 0.0012554583 | 40.000004 | 9 |
ATCGGGT | 20 | 0.0012554583 | 40.000004 | 4 |
CGTCCGA | 40 | 1.9266736E-8 | 40.000004 | 40 |
CCGCAAA | 20 | 0.0012554583 | 40.000004 | 38 |
ACGGTAT | 20 | 0.0012554583 | 40.000004 | 5 |
TAAACGG | 50 | 7.8216544E-11 | 40.0 | 2 |
ACGTATG | 35 | 3.054265E-7 | 40.0 | 1 |
TACCGAC | 45 | 1.2205419E-9 | 40.0 | 4 |
TACGCAT | 130 | 0.0 | 38.46154 | 16 |
ACCGCTA | 125 | 0.0 | 38.4 | 26 |
GCGATGT | 110 | 0.0 | 38.181816 | 9 |
TCCCTAC | 325 | 0.0 | 37.53846 | 5 |
CTCGATC | 155 | 0.0 | 37.419353 | 16 |
TACACGA | 265 | 0.0 | 36.981133 | 9 |
GCCGATA | 55 | 2.1645974E-10 | 36.363636 | 9 |
TCGATCA | 155 | 0.0 | 36.129032 | 17 |
GGGCGTA | 50 | 3.421519E-9 | 36.0 | 7 |
CTACGCG | 50 | 3.421519E-9 | 36.0 | 1 |
CTACCGA | 50 | 3.421519E-9 | 36.0 | 3 |
CCGCTAC | 145 | 0.0 | 35.86207 | 27 |