FastQCFastQC Report
Sat 18 Jun 2016
SRR3553722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721912
Sequences flagged as poor quality0
Sequence length46
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31100.43080042996930373No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT17000.23548576557807602No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.2347931603851993No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT14290.19794656412415917No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT12690.17578319795210495No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG12290.1702423564090914No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCG9700.13436540741807868No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTA8990.12453041367922961No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7770.10763084697303826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG200.001255458340.0000049
ATCGGGT200.001255458340.0000044
CGTCCGA401.9266736E-840.00000440
CCGCAAA200.001255458340.00000438
ACGGTAT200.001255458340.0000045
TAAACGG507.8216544E-1140.02
ACGTATG353.054265E-740.01
TACCGAC451.2205419E-940.04
TACGCAT1300.038.4615416
ACCGCTA1250.038.426
GCGATGT1100.038.1818169
TCCCTAC3250.037.538465
CTCGATC1550.037.41935316
TACACGA2650.036.9811339
GCCGATA552.1645974E-1036.3636369
TCGATCA1550.036.12903217
GGGCGTA503.421519E-936.07
CTACGCG503.421519E-936.01
CTACCGA503.421519E-936.03
CCGCTAC1450.035.8620727