##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553722_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 721912 Sequences flagged as poor quality 0 Sequence length 46 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.736377841066503 31.0 31.0 34.0 30.0 34.0 2 31.895088321014196 31.0 31.0 34.0 30.0 34.0 3 31.83929481709682 31.0 31.0 34.0 30.0 34.0 4 35.62987455534746 37.0 35.0 37.0 33.0 37.0 5 33.74090055297599 37.0 35.0 37.0 30.0 37.0 6 34.580318653797136 37.0 35.0 37.0 30.0 37.0 7 35.53710424539279 37.0 35.0 37.0 33.0 37.0 8 35.643337415086606 37.0 35.0 37.0 35.0 37.0 9 37.46315755937012 39.0 37.0 39.0 35.0 39.0 10 36.743204157847494 39.0 37.0 39.0 32.0 39.0 11 36.45680221412028 39.0 35.0 39.0 32.0 39.0 12 36.60988319906027 39.0 35.0 39.0 32.0 39.0 13 32.84415967597159 38.0 35.0 39.0 8.0 39.0 14 35.10568324117067 39.0 34.0 40.0 17.0 41.0 15 36.57187995212713 39.0 35.0 41.0 30.0 41.0 16 37.255716763262 39.0 36.0 41.0 32.0 41.0 17 37.4134645219916 39.0 36.0 40.0 32.0 41.0 18 37.54164080940613 39.0 36.0 40.0 33.0 41.0 19 37.54427686477022 39.0 36.0 40.0 32.0 41.0 20 37.56409368454881 39.0 36.0 41.0 33.0 41.0 21 37.47628242777513 39.0 36.0 41.0 32.0 41.0 22 37.4943123261561 39.0 36.0 41.0 33.0 41.0 23 37.36217987787985 39.0 35.0 40.0 32.0 41.0 24 37.3353400414455 39.0 35.0 41.0 32.0 41.0 25 37.15991838340407 39.0 35.0 40.0 32.0 41.0 26 37.141226354458716 39.0 35.0 40.0 32.0 41.0 27 36.738095501944834 39.0 35.0 40.0 31.0 41.0 28 36.798010006759824 39.0 35.0 40.0 31.0 41.0 29 36.88701947051718 39.0 35.0 40.0 31.0 41.0 30 36.402190294661956 39.0 35.0 40.0 30.0 41.0 31 36.48926877514157 39.0 35.0 40.0 30.0 41.0 32 36.40740145613316 38.0 35.0 40.0 30.0 41.0 33 36.383286328527575 38.0 35.0 40.0 30.0 41.0 34 36.32553967796629 38.0 35.0 40.0 30.0 41.0 35 36.26411944946199 38.0 35.0 40.0 30.0 41.0 36 36.13528241669345 38.0 35.0 40.0 30.0 41.0 37 36.13693635789404 38.0 35.0 40.0 30.0 41.0 38 35.960226454193865 38.0 35.0 40.0 30.0 41.0 39 35.92147242323164 38.0 35.0 40.0 30.0 41.0 40 35.80290811068385 38.0 35.0 40.0 29.0 41.0 41 35.63243026850918 38.0 34.0 40.0 29.0 41.0 42 35.57348125533306 38.0 34.0 40.0 29.0 41.0 43 35.49269994126708 38.0 34.0 40.0 28.0 41.0 44 35.444392114274315 38.0 34.0 40.0 28.0 41.0 45 35.316087002293905 37.0 34.0 40.0 28.0 41.0 46 34.12308840966766 36.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 11.0 10 22.0 11 15.0 12 7.0 13 14.0 14 10.0 15 23.0 16 35.0 17 83.0 18 167.0 19 325.0 20 564.0 21 923.0 22 1488.0 23 2146.0 24 3092.0 25 4401.0 26 5872.0 27 7764.0 28 10024.0 29 12413.0 30 16332.0 31 21399.0 32 28571.0 33 39761.0 34 57132.0 35 72116.0 36 82810.0 37 109781.0 38 135087.0 39 109520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.62593224658961 23.186482562971662 21.083733197398022 19.103851993040703 2 26.418178392934315 26.33645098017487 22.610789126652556 24.634581500238255 3 28.163543478983588 27.876527887055484 22.362974988641273 21.59695364531965 4 23.816753288489455 28.177118540763974 22.49927969059941 25.506848480147166 5 20.883570296656657 35.270780926207074 20.192073272088564 23.653575505047705 6 19.311495029865135 39.23165150323031 21.646267135052472 19.810586331852083 7 83.9736699209876 4.892840124558118 7.15281086891477 3.9806790855395118 8 83.73084254036503 4.72606079411341 6.807339398707876 4.735757266813684 9 78.10979731601635 6.546781325147663 9.746478795199415 5.596942563636565 10 35.424816321102846 28.61997030108933 17.518340185507377 18.436873192300446 11 30.235402652954928 27.206501623466572 22.366160972528508 20.19193475104999 12 23.534724453950066 26.094316204745176 28.10384091135762 22.267118429947143 13 20.973331929653476 33.83251698267933 27.408049734593693 17.786101353073505 14 21.754590587218388 29.278776360553643 28.25593701171334 20.710696040514634 15 20.900192821285696 26.614462704595574 30.69584658517936 21.789497888939373 16 23.300900940834893 26.542985848690698 27.833170801981407 22.322942408493002 17 21.438208535112313 26.15637363002693 27.4433725994304 24.96204523543036 18 25.498675738871217 23.311844102882347 27.521221423109743 23.668258735136693 19 23.53555558018152 27.65752612506788 25.247121532818404 23.5597967619322 20 27.59380644732322 25.893460698810934 25.067598266824763 21.44513458704108 21 26.471508992785825 27.85505712607631 25.223295914183446 20.450137966954422 22 25.583727656556476 22.839764403417593 25.83209587872206 25.744412061303873 23 23.313367834306675 28.4437715400215 25.51737607907889 22.725484546592938 24 22.867330090094086 25.41223861080021 28.773036048715078 22.947395250390628 25 22.721744478551404 27.579123217234237 26.410975298928403 23.28815700528596 26 22.43320515519897 26.395045379492238 27.14208934052904 24.02966012477975 27 23.59705892130897 25.073000587329204 26.711842994714036 24.618097496647792 28 20.406365318764614 27.64907634171478 27.830261860171323 24.114296479349285 29 21.546670508316804 26.600472079699465 27.03958377198329 24.813273640000443 30 23.930894624275535 25.061364820088876 28.7925675151542 22.21517304048139 31 25.42609071465774 25.541478739791003 25.450608938485576 23.58182160706568 32 23.130797105464378 25.882517536763483 27.185584946641693 23.801100411130445 33 21.60498786555702 25.207504515785857 25.32843338246213 27.859074236194992 34 21.01184077837742 26.242395194982215 29.251072152838574 23.49469187380179 35 21.415214042708808 26.614462704595574 27.779701681091325 24.190621571604296 36 22.768426068551292 29.701404049247 25.294634249049746 22.23553563315196 37 22.617022573388446 26.81601081572269 26.393244605990756 24.173722004898103 38 21.698351045556798 28.96142466117754 25.277457640266405 24.062766652999258 39 23.902082248251865 25.624175799820474 23.885182681545672 26.588559270381985 40 25.854813329048415 24.216663526856458 26.87792971996587 23.050593424129257 41 20.69975287846718 27.18073671029156 25.917701880561623 26.20180853067964 42 23.29425193098328 26.73400636088609 26.524424029521604 23.44731767860903 43 22.680049645940226 24.383165815224018 27.73205044382141 25.204734095014352 44 23.382628353594345 24.590947373087026 26.79772603863075 25.228698234687887 45 23.79791442724321 23.288295526324536 26.522069171865827 26.391720874566428 46 25.125084497833534 24.055563558993338 25.527072551779163 25.29227939139396 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3581.0 1 3235.5 2 2890.0 3 3607.0 4 4324.0 5 3128.5 6 1933.0 7 1933.0 8 1871.5 9 1810.0 10 1879.5 11 1949.0 12 1911.0 13 1873.0 14 1889.5 15 1906.0 16 1910.0 17 1914.0 18 1884.0 19 1854.0 20 1854.0 21 1934.0 22 2014.0 23 2337.0 24 2660.0 25 3005.5 26 3351.0 27 3998.0 28 4645.0 29 5918.5 30 7192.0 31 7192.0 32 8462.0 33 9732.0 34 11861.5 35 13991.0 36 15669.0 37 17347.0 38 20407.0 39 23467.0 40 28140.0 41 32813.0 42 38755.5 43 44698.0 44 44698.0 45 54112.0 46 63526.0 47 67314.5 48 71103.0 49 67893.5 50 64684.0 51 62325.0 52 59966.0 53 57042.0 54 54118.0 55 51342.5 56 48567.0 57 48567.0 58 44290.5 59 40014.0 60 36851.5 61 33689.0 62 30184.5 63 26680.0 64 23638.5 65 20597.0 66 18062.0 67 15527.0 68 13584.0 69 11641.0 70 11641.0 71 10566.0 72 9491.0 73 7881.5 74 6272.0 75 5255.0 76 4238.0 77 3603.5 78 2969.0 79 2401.5 80 1834.0 81 1834.0 82 1237.0 83 640.0 84 455.5 85 271.0 86 175.0 87 79.0 88 61.0 89 43.0 90 28.5 91 14.0 92 9.0 93 4.0 94 4.0 95 2.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 46 721912.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.709262650999975 #Duplication Level Percentage of deduplicated Percentage of total 1 75.58953620638027 26.236570658545254 2 9.639576415710536 6.691651793145641 3 3.710271133045361 3.863423257898842 4 1.9801976640556358 2.7492480329041475 5 1.2196335322185106 2.1166290303869566 6 0.823483094823327 1.7149494616128709 7 0.6188712884767978 1.5036396269232768 8 0.49097304518294926 1.3633049903853012 9 0.3984784660841547 1.2447804366074262 >10 5.183106764463137 40.69378624665041 >50 0.29046948986239196 6.266487384803857 >100 0.04827966973592085 3.0126444967270842 >500 0.004748819974025002 1.1099794910888419 >1k 0.002374409987012501 1.4329050923201085 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3110 0.43080042996930373 No Hit GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT 1700 0.23548576557807602 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1695 0.2347931603851993 No Hit CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT 1429 0.19794656412415917 No Hit CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT 1269 0.17578319795210495 No Hit GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG 1229 0.1702423564090914 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCG 970 0.13436540741807868 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTA 899 0.12453041367922961 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 777 0.10763084697303826 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.20168663216569332 0.0 2 0.0 0.0 0.0 0.8546748080098405 0.0 3 0.0 0.0 0.0 1.1157869657243542 0.0 4 0.0 0.0 0.0 1.4766342712131117 0.0 5 0.0 0.0 0.0 2.421901838451224 0.0 6 0.0 0.0 0.0 3.306635711831913 0.0 7 0.0 0.0 0.0 3.912526734560445 0.0 8 0.0 0.0 0.0 5.054494176575538 0.0 9 0.0 0.0 0.0 5.488757632509225 0.0 10 0.0 0.0 0.0 6.515891133545363 0.0 11 0.0 0.0 0.0 7.598571571050211 0.0 12 0.0 0.0 0.0 8.59716973813983 0.0 13 0.0 0.0 0.0 8.99652589235253 0.0 14 0.0 0.0 0.0 9.209709770719977 0.0 15 0.0 0.0 0.0 9.524291049324571 0.0 16 0.0 0.0 0.0 10.167859794545596 0.0 17 0.0 0.0 0.0 10.951334788727712 0.0 18 0.0 0.0 0.0 11.801161360387416 0.0 19 0.0 0.0 0.0 12.267423176232006 0.0 20 0.0 0.0 0.0 12.688388612462461 0.0 21 0.0 0.0 0.0 13.237070446259377 0.0 22 0.0 0.0 0.0 13.755416172608296 0.0 23 0.0 0.0 0.0 14.32972439854165 0.0 24 0.0 0.0 0.0 14.792107625306132 0.0 25 0.0 0.0 0.0 15.202822504682011 0.0 26 0.0 0.0 0.0 15.567271357173727 0.0 27 0.0 0.0 0.0 15.975908420970978 0.0 28 0.0 0.0 0.0 16.36875408637064 0.0 29 0.0 0.0 0.0 16.81617704096898 0.0 30 0.0 0.0 0.0 17.345465929365353 0.0 31 0.0 0.0 0.0 17.75271778277685 0.0 32 0.0 0.0 0.0 18.18144039716752 0.0 33 0.0 0.0 0.0 18.62997152007447 0.0 34 0.0 0.0 0.0 19.05730892407939 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGATCG 20 0.0012554583 40.000004 9 ATCGGGT 20 0.0012554583 40.000004 4 CGTCCGA 40 1.9266736E-8 40.000004 40 CCGCAAA 20 0.0012554583 40.000004 38 ACGGTAT 20 0.0012554583 40.000004 5 TAAACGG 50 7.8216544E-11 40.0 2 ACGTATG 35 3.054265E-7 40.0 1 TACCGAC 45 1.2205419E-9 40.0 4 TACGCAT 130 0.0 38.46154 16 ACCGCTA 125 0.0 38.4 26 GCGATGT 110 0.0 38.181816 9 TCCCTAC 325 0.0 37.53846 5 CTCGATC 155 0.0 37.419353 16 TACACGA 265 0.0 36.981133 9 GCCGATA 55 2.1645974E-10 36.363636 9 TCGATCA 155 0.0 36.129032 17 GGGCGTA 50 3.421519E-9 36.0 7 CTACGCG 50 3.421519E-9 36.0 1 CTACCGA 50 3.421519E-9 36.0 3 CCGCTAC 145 0.0 35.86207 27 >>END_MODULE