##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	847021
Sequences flagged as poor quality	0
Sequence length	46
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.565657758190174	31.0	31.0	34.0	30.0	34.0
2	31.70857511206924	31.0	31.0	34.0	30.0	34.0
3	31.681219237775686	31.0	31.0	34.0	30.0	34.0
4	35.47523733177808	37.0	35.0	37.0	33.0	37.0
5	33.60868384609118	37.0	35.0	37.0	30.0	37.0
6	34.409323971896804	37.0	35.0	37.0	30.0	37.0
7	35.38170364135009	37.0	35.0	37.0	32.0	37.0
8	35.45642788077273	37.0	35.0	37.0	33.0	37.0
9	37.249945396867375	39.0	37.0	39.0	34.0	39.0
10	36.53576593732623	38.0	35.0	39.0	32.0	39.0
11	36.26301591105769	38.0	35.0	39.0	32.0	39.0
12	36.46028138617579	38.0	35.0	39.0	32.0	39.0
13	32.90637894455982	38.0	34.0	39.0	10.0	39.0
14	35.08982658045078	39.0	34.0	40.0	17.0	41.0
15	36.439353923928685	39.0	34.0	40.0	30.0	41.0
16	37.09825966534478	39.0	36.0	40.0	32.0	41.0
17	37.27604628456673	39.0	36.0	40.0	32.0	41.0
18	37.39337985717001	39.0	36.0	40.0	32.0	41.0
19	37.39300560434747	39.0	36.0	40.0	32.0	41.0
20	37.38109208626469	39.0	36.0	40.0	32.0	41.0
21	37.3174785512992	39.0	36.0	40.0	32.0	41.0
22	37.33498224955461	39.0	36.0	40.0	32.0	41.0
23	37.22680665532496	39.0	35.0	40.0	32.0	41.0
24	37.199140281055605	39.0	35.0	40.0	32.0	41.0
25	37.00640125805617	39.0	35.0	40.0	31.0	41.0
26	36.955097925553204	39.0	35.0	40.0	31.0	41.0
27	36.56447596930891	39.0	35.0	40.0	31.0	41.0
28	36.618585607676785	39.0	35.0	40.0	30.0	41.0
29	36.700718164012464	39.0	35.0	40.0	30.0	41.0
30	36.277053343423596	39.0	35.0	40.0	30.0	41.0
31	36.36513380423862	39.0	35.0	40.0	30.0	41.0
32	36.30587199136739	38.0	35.0	40.0	30.0	41.0
33	36.28544156520322	38.0	35.0	40.0	30.0	41.0
34	36.312511732294716	38.0	35.0	40.0	30.0	41.0
35	36.25870314903645	38.0	35.0	40.0	30.0	41.0
36	36.134637748060555	38.0	35.0	40.0	30.0	41.0
37	36.08302745740661	38.0	35.0	40.0	30.0	41.0
38	35.95054313883599	38.0	35.0	40.0	29.0	41.0
39	35.88896969496624	38.0	35.0	40.0	29.0	41.0
40	35.82022051401323	38.0	35.0	40.0	29.0	41.0
41	35.65731192024755	38.0	34.0	40.0	28.0	41.0
42	35.59941252932336	38.0	34.0	40.0	28.0	41.0
43	35.46317269583635	38.0	34.0	40.0	27.0	41.0
44	35.41760593893186	38.0	34.0	40.0	27.0	41.0
45	35.38207317173954	38.0	34.0	40.0	27.0	41.0
46	34.15388874656	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	20.0
10	20.0
11	15.0
12	17.0
13	9.0
14	20.0
15	30.0
16	37.0
17	106.0
18	198.0
19	380.0
20	721.0
21	1192.0
22	1860.0
23	2774.0
24	4002.0
25	5525.0
26	7525.0
27	9808.0
28	13041.0
29	16661.0
30	21096.0
31	27418.0
32	35299.0
33	47520.0
34	65731.0
35	81798.0
36	94702.0
37	125642.0
38	154802.0
39	129049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.23870246428365	21.679155534514493	21.990009692793922	22.092132308407937
2	23.499653491471875	28.723727038644853	23.651479715378958	24.125139754504314
3	26.57738119834101	28.001903140536065	23.379703690935642	22.04101197018728
4	24.21557434821569	27.70155639588629	23.429997603365205	24.652871652532816
5	20.464073499948643	34.17282452265056	20.843520998889048	24.519580978511748
6	19.28570838267292	39.53857106258286	22.265681724538116	18.910038830206098
7	81.47944383905475	5.104359868291341	8.793052356435084	4.623143936218819
8	80.67934561244644	5.451930943860896	7.960841584801321	5.907881858891338
9	74.84902971709084	6.678346817847491	11.019797620129843	7.4528258449318265
10	35.08767787339393	25.771379930367722	19.271304961742388	19.869637234495958
11	27.75763528885352	26.843136120592053	24.17909355258016	21.220135037974266
12	24.07307492966526	22.908522929183576	30.91942230475986	22.098979836391305
13	20.565605811426163	30.529585453017106	29.898904513583492	19.005904221973243
14	20.374701453682967	28.509682758750966	28.956070746770152	22.15954504079592
15	19.06399014900457	26.91468098193551	33.00059856839441	21.02073030066551
16	20.44353091599854	24.744014611207987	31.024024197747163	23.78843027504631
17	19.568227942400483	25.603851616429818	28.57107438894667	26.256846052223025
18	20.723453137525517	25.225702786589704	29.589349024404356	24.461495051480423
19	21.93947965870976	28.09434476831153	27.277363843399392	22.688811729579314
20	24.511552842255387	25.81199285495873	27.201804913927752	22.47464938885813
21	24.19656655502048	27.696597841139713	27.19424902098059	20.912586582859223
22	21.739720738919104	24.41828478868883	27.19389483849869	26.648099633893374
23	20.926635821307855	27.537215724285467	27.265203578187553	24.270944876219126
24	22.075485731758718	25.13066382061366	28.496814128575327	24.2970363190523
25	21.649050023553134	26.92270911819187	26.231699096008242	25.196541762246742
26	19.853580961983234	27.351978286252642	27.352214407907244	25.442226343856884
27	21.71540020849542	26.774424719103777	26.641252105910006	24.868922966490796
28	19.21404546050216	26.892721668057817	29.44401614599874	24.449216725441282
29	20.790629748258898	26.042093407365343	26.67194792100786	26.4953289233679
30	21.62472949312945	24.98887276702703	27.752558673279648	25.633839066563873
31	22.079853982368796	25.736315864659794	25.6506037040404	26.533226448931018
32	22.606287211297005	27.49636667803986	25.648714730803608	24.248631379859532
33	20.783073855311734	25.128774847376867	27.137461763049558	26.95068953426184
34	20.091827711473506	24.689234387341045	28.446166033663868	26.772771867521584
35	21.027459767821576	24.89147258450499	26.07857420300087	28.002493444672567
36	21.836530617304646	24.818038749924735	28.295874600511677	25.04955603225894
37	20.51767311554259	26.0520105168585	28.78051429657588	24.649802071023032
38	21.13277002577268	26.952224325016733	28.441443600571887	23.473562048638698
39	21.650348692653427	24.518990674375253	28.933875311237855	24.896785321733464
40	23.229530318610756	22.98124839880003	29.900439304338384	23.888781978250833
41	20.30858739039528	24.079568275166732	28.656432367084168	26.95541196735382
42	22.392242931403118	24.231276438246514	28.547108041004886	24.829372589345482
43	21.699580057637295	23.203674997432177	30.127470275235208	24.96927466969532
44	21.17456355863668	24.454293341015156	29.24413916538079	25.127003934967373
45	21.645862381216048	23.548530673973843	27.672867614852525	27.132739329957577
46	23.300248754163118	23.8543082166794	27.627768378824136	25.217674650333343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3212.0
1	3158.0
2	3104.0
3	4069.0
4	5034.0
5	3631.0
6	2228.0
7	2228.0
8	2141.0
9	2054.0
10	2009.5
11	1965.0
12	2041.0
13	2117.0
14	2020.0
15	1923.0
16	1916.0
17	1909.0
18	2013.0
19	2117.0
20	2117.0
21	2084.5
22	2052.0
23	2560.0
24	3068.0
25	4019.0
26	4970.0
27	5910.5
28	6851.0
29	8584.0
30	10317.0
31	10317.0
32	12580.5
33	14844.0
34	16267.5
35	17691.0
36	20610.5
37	23530.0
38	26134.0
39	28738.0
40	33144.0
41	37550.0
42	45786.5
43	54023.0
44	54023.0
45	61479.5
46	68936.0
47	81855.5
48	94775.0
49	93670.0
50	92565.0
51	85569.5
52	78574.0
53	68749.0
54	58924.0
55	54836.5
56	50749.0
57	50749.0
58	46189.5
59	41630.0
60	38945.5
61	36261.0
62	32137.5
63	28014.0
64	24636.0
65	21258.0
66	18096.0
67	14934.0
68	12886.0
69	10838.0
70	10838.0
71	9147.0
72	7456.0
73	6240.5
74	5025.0
75	4271.5
76	3518.0
77	2766.0
78	2014.0
79	1567.5
80	1121.0
81	1121.0
82	873.0
83	625.0
84	464.5
85	304.0
86	226.0
87	148.0
88	91.0
89	34.0
90	21.0
91	8.0
92	5.5
93	3.0
94	3.0
95	5.0
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	847021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.13344111836491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51053181677818	28.410993281531173
2	9.593952437962528	7.125129358949499
3	3.6479653058020673	4.063845146545172
4	1.894674644924041	2.814231574629832
5	1.1047476649493306	2.051154118352355
6	0.759624686489142	1.6924487140680577
7	0.5698762021174442	1.4813025077259867
8	0.4617718719255048	1.3717742893010298
9	0.4062974879136552	1.3578501459583165
>10	4.844930672721583	39.85253488143626
>50	0.1602452868704629	3.7009060003007734
>100	0.038496388621863734	2.580660370441724
>500	0.004381702769964204	1.11849923013366
>1k	0.0025038301542652593	2.3786703806261036
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	3683	0.43481802694384203	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT	2766	0.3265562483102544	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2750	0.3246672750734634	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTA	2627	0.3101457933156321	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCG	2444	0.28854066191983435	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2269	0.26788001714243215	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT	2263	0.2671716521786355	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTT	1665	0.19657127745356962	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT	904	0.10672698787869486	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGT	901	0.10637280539679654	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3612165459888245E-4	0.0	0.0	0.23482298549858857	0.0
2	2.3612165459888245E-4	0.0	0.0	1.1241751975452792	0.0
3	2.3612165459888245E-4	0.0	0.0	1.471982394769433	0.0
4	2.3612165459888245E-4	0.0	0.0	2.0112842538732805	0.0
5	2.3612165459888245E-4	0.0	0.0	3.4970797654367485	0.0
6	2.3612165459888245E-4	0.0	0.0	4.744392405855344	0.0
7	2.3612165459888245E-4	0.0	0.0	5.718276170248435	0.0
8	2.3612165459888245E-4	0.0	0.0	7.249170917840289	0.0
9	2.3612165459888245E-4	0.0	0.0	7.80889730006694	0.0
10	3.5418248189832363E-4	0.0	0.0	9.212994719139195	0.0
11	3.5418248189832363E-4	0.0	0.0	10.61921723310284	0.0
12	3.5418248189832363E-4	0.0	0.0	12.109262934449086	0.0
13	3.5418248189832363E-4	0.0	0.0	12.635105859240799	0.0
14	3.5418248189832363E-4	0.0	0.0	12.932264961553491	0.0
15	3.5418248189832363E-4	0.0	0.0	13.318205805995365	0.0
16	3.5418248189832363E-4	0.0	0.0	14.118658215085576	0.0
17	3.5418248189832363E-4	0.0	0.0	15.034101869965443	0.0
18	3.5418248189832363E-4	0.0	0.0	16.06973144703614	0.0
19	3.5418248189832363E-4	0.0	0.0	16.63831239131025	0.0
20	3.5418248189832363E-4	0.0	0.0	17.150578320962527	0.0
21	3.5418248189832363E-4	0.0	0.0	17.779960591295847	0.0
22	3.5418248189832363E-4	0.0	0.0	18.41949609277692	0.0
23	3.5418248189832363E-4	0.0	0.0	19.11440212226143	0.0
24	3.5418248189832363E-4	0.0	0.0	19.680031545853055	0.0
25	3.5418248189832363E-4	0.0	0.0	20.137399190811088	0.0
26	3.5418248189832363E-4	0.0	0.0	20.55970277006119	0.0
27	3.5418248189832363E-4	0.0	0.0	20.976929733737418	0.0
28	3.5418248189832363E-4	0.0	0.0	21.42792209402128	0.0
29	3.5418248189832363E-4	0.0	0.0	21.898984794946053	0.0
30	3.5418248189832363E-4	0.0	0.0	22.436987984949607	0.0
31	3.5418248189832363E-4	0.0	0.0	22.931662851334263	0.0
32	4.722433091977649E-4	0.0	0.0	23.38631509726441	0.0
33	4.722433091977649E-4	0.0	0.0	23.8541901558521	0.0
34	4.722433091977649E-4	0.0	0.0	24.304710272826767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTCGA	30	4.865995E-6	40.000004	36
ATACGAG	30	4.865995E-6	40.000004	11
GTTAACG	20	0.0012555816	40.0	1
GTCGTTA	20	0.0012555816	40.0	9
GTATACG	35	3.0551018E-7	40.0	1
CGGTCTA	50	7.8216544E-11	40.0	31
TACGTAG	45	1.2205419E-9	40.0	1
TACGAAT	105	0.0	38.09524	12
GCTACGA	105	0.0	38.09524	10
CGGGTAT	135	0.0	37.037037	6
ACGTAGG	130	0.0	36.923077	2
CTATACG	60	1.4551915E-11	36.666668	1
TACGCAT	105	0.0	36.19048	16
CTAGACG	50	3.423338E-9	36.0	1
TCGTGCG	50	3.423338E-9	36.0	1
CTACGCG	50	3.423338E-9	36.0	1
GCGTAAG	50	3.423338E-9	36.0	1
ATCCCTA	325	0.0	35.692307	4
TACACGA	265	0.0	35.4717	9
TGGGCGA	535	0.0	35.140186	6
>>END_MODULE