##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	712759
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62382796990287	31.0	31.0	34.0	30.0	34.0
2	31.783694067700303	31.0	31.0	34.0	30.0	34.0
3	31.739896655110634	31.0	31.0	34.0	30.0	34.0
4	35.53946705688739	37.0	35.0	37.0	33.0	37.0
5	33.62853923977109	37.0	35.0	37.0	30.0	37.0
6	34.45483536510938	37.0	35.0	37.0	30.0	37.0
7	35.424205095972134	37.0	35.0	37.0	32.0	37.0
8	35.50274216109513	37.0	35.0	37.0	33.0	37.0
9	37.3078305570326	39.0	37.0	39.0	34.0	39.0
10	36.645386448996085	38.0	35.0	39.0	32.0	39.0
11	36.3778331806403	38.0	35.0	39.0	32.0	39.0
12	36.53137596298328	38.0	35.0	39.0	32.0	39.0
13	32.8839607777664	38.0	35.0	39.0	10.0	39.0
14	35.12039553341312	39.0	34.0	40.0	17.0	41.0
15	36.50455343250664	39.0	35.0	40.0	30.0	41.0
16	37.172159173016404	39.0	36.0	40.0	32.0	41.0
17	37.34651965110226	39.0	36.0	40.0	32.0	41.0
18	37.46947425427108	39.0	36.0	40.0	32.0	41.0
19	37.47886873403212	39.0	36.0	40.0	32.0	41.0
20	37.47882243507272	39.0	36.0	40.0	32.0	41.0
21	37.422654782331755	39.0	36.0	40.0	32.0	41.0
22	37.44492458180114	39.0	36.0	40.0	32.0	41.0
23	37.33564360464056	39.0	36.0	40.0	32.0	41.0
24	37.315483915320605	39.0	35.0	40.0	32.0	41.0
25	37.1299401340425	39.0	35.0	40.0	32.0	41.0
26	37.11071063290677	39.0	35.0	40.0	32.0	41.0
27	36.71093314851163	39.0	35.0	40.0	31.0	41.0
28	36.77896455884808	39.0	35.0	40.0	31.0	41.0
29	36.87010897091443	39.0	35.0	40.0	31.0	41.0
30	36.413430065421835	39.0	35.0	40.0	30.0	41.0
31	36.47763128911736	39.0	35.0	40.0	30.0	41.0
32	36.458669760746616	39.0	35.0	40.0	30.0	41.0
33	36.447821774260305	39.0	35.0	40.0	30.0	41.0
34	36.4117857508639	39.0	35.0	40.0	30.0	41.0
35	36.40411836258819	39.0	35.0	40.0	30.0	41.0
36	36.295797036586	38.0	35.0	40.0	30.0	41.0
37	36.220235731853265	38.0	35.0	40.0	30.0	41.0
38	36.14681119424658	38.0	35.0	40.0	30.0	41.0
39	36.112991908906096	38.0	35.0	40.0	30.0	41.0
40	36.01038078789605	38.0	35.0	40.0	30.0	41.0
41	35.8587039939166	38.0	35.0	40.0	29.0	41.0
42	35.803490380338935	38.0	35.0	40.0	29.0	41.0
43	35.70878235139788	38.0	35.0	40.0	29.0	41.0
44	35.646267532223376	38.0	34.0	40.0	29.0	41.0
45	35.579197176044076	38.0	34.0	40.0	29.0	41.0
46	34.38773273995839	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	16.0
11	14.0
12	16.0
13	10.0
14	14.0
15	24.0
16	52.0
17	76.0
18	156.0
19	284.0
20	507.0
21	919.0
22	1391.0
23	2114.0
24	2933.0
25	4153.0
26	5532.0
27	7507.0
28	9568.0
29	12422.0
30	16319.0
31	21529.0
32	28973.0
33	39638.0
34	55961.0
35	70400.0
36	81311.0
37	108032.0
38	134008.0
39	108870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.99101238988214	23.081574557459113	22.13370858873757	20.793704463921184
2	25.152260441467593	28.016763029298826	22.35888989125357	24.472086637980016
3	27.720589989042583	27.48600859477046	22.8570947543279	21.936306661859057
4	24.79323305633461	28.519317188558823	23.097849343186123	23.58960041192044
5	20.819098741650404	34.20959959818115	20.964449414177864	24.006852245990583
6	19.093550554956163	40.227762820252	21.869944820058393	18.808741804733437
7	81.1138126631863	5.527815152106111	8.516062231413422	4.842309953294171
8	80.27425819947555	5.582391804242388	8.003687080766431	6.139662915515623
9	74.2391186922929	7.062695806015778	11.370884127734621	7.327301373956694
10	35.19885403060501	26.11205189973048	18.830909185292644	19.85818488437186
11	29.024256445727097	26.35729608465133	23.07750586102736	21.540941608594206
12	23.379150596484926	24.19303018271253	29.97450751235691	22.453311708445632
13	20.55561557272514	31.44190392545026	29.689839062011142	18.312641439813458
14	20.85964540609098	28.40174589166885	28.90696574859104	21.83164295364913
15	19.185867873993875	27.198534146885557	32.59573011354469	21.019867865575883
16	20.46329825368743	25.298593213133753	30.516906836672703	23.72120169650611
17	20.196167287961288	25.94299054799729	28.28038649810104	25.580455665940384
18	22.112383007440105	25.111713777027017	29.110540870055658	23.665362345477224
19	22.630510453042334	27.640198159546216	26.81144678636117	22.917844601050284
20	24.89032057118886	25.568109276768165	26.895767012412332	22.645803139630647
21	24.603687922565694	27.54802114038546	27.06847616094641	20.77981477610244
22	22.20778692377087	24.53999177842721	26.584020685813858	26.668200611988063
23	21.127477871201908	27.65506994650366	26.632985342871855	24.584466839422582
24	21.649533713358935	25.504974332137508	28.901213453635798	23.944278500867753
25	21.502218842554075	27.423434849647638	26.284620748387606	24.789725559410687
26	21.12972266923322	26.741437147759623	27.627851770373997	24.500988412633163
27	22.770249130491514	26.606749265880893	26.927895684235487	23.69510591939211
28	20.214265972088743	27.418664653831097	28.467686833838645	23.89938254024151
29	21.924802071948584	25.066958116277732	28.597744819777795	24.410494991995893
30	23.39486418270411	25.620862030504	28.422229673704575	22.562044113087314
31	24.043610813753315	28.036264712195845	24.75619388881796	23.16393058523288
32	22.421603936253348	27.005762115946624	27.751175362219204	22.82145858558082
33	22.23065580371486	25.554219588949422	26.74887304123834	25.466251566097377
34	20.55954396927994	26.947257067255553	29.299945703947618	23.19325325951689
35	21.36458466325925	24.740480302598776	28.358533529566092	25.536401504575878
36	22.61381476768445	27.39046437856274	26.165085253220234	23.83063560053258
37	20.797071660968154	28.06755158475726	27.658858043181496	23.476518711093092
38	19.833913005658292	28.035843812564977	27.35735360760089	24.772889574175842
39	21.35883236830401	24.481486729736137	28.128301431479645	26.031379470480204
40	22.059349653950353	23.724288293799166	30.551841506035	23.66452054621548
41	19.728828397817495	25.225637277116107	27.18871315549856	27.856821169567837
42	21.7269792454392	24.831955822374745	27.3586163064935	26.082448625692557
43	22.081657334386517	23.51117278070147	28.5729117415564	25.83425814335561
44	22.250017186734926	23.88493165291494	28.695674133893785	25.169377026456345
45	22.11939800128795	23.087467152291307	28.72064751199213	26.072487334428608
46	23.55312244391162	24.31873887246601	27.585621507409936	24.542517176212435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3019.0
1	2808.0
2	2597.0
3	3274.0
4	3951.0
5	2866.5
6	1782.0
7	1782.0
8	1751.5
9	1721.0
10	1758.0
11	1795.0
12	1814.0
13	1833.0
14	1774.5
15	1716.0
16	1695.5
17	1675.0
18	1717.5
19	1760.0
20	1760.0
21	1879.0
22	1998.0
23	2508.0
24	3018.0
25	3507.5
26	3997.0
27	4604.5
28	5212.0
29	6614.0
30	8016.0
31	8016.0
32	9395.0
33	10774.0
34	12335.0
35	13896.0
36	16587.5
37	19279.0
38	23014.5
39	26750.0
40	32906.5
41	39063.0
42	46247.5
43	53432.0
44	53432.0
45	64536.5
46	75641.0
47	76482.5
48	77324.0
49	70737.5
50	64151.0
51	59476.0
52	54801.0
53	49232.5
54	43664.0
55	41509.0
56	39354.0
57	39354.0
58	36064.0
59	32774.0
60	31252.0
61	29730.0
62	27364.5
63	24999.0
64	21733.0
65	18467.0
66	16062.5
67	13658.0
68	12377.5
69	11097.0
70	11097.0
71	9380.0
72	7663.0
73	6118.5
74	4574.0
75	3936.0
76	3298.0
77	2676.0
78	2054.0
79	1561.5
80	1069.0
81	1069.0
82	835.5
83	602.0
84	452.5
85	303.0
86	244.5
87	186.0
88	112.0
89	38.0
90	30.0
91	22.0
92	12.5
93	3.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	712759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.4215670129824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43375974442311	27.4741573557272
2	9.996809074209077	7.281989032245928
3	3.7563910935536358	4.104409498225019
4	1.973745136367909	2.8754756300308753
5	1.2159766917839967	2.214388828301774
6	0.8126841052760488	1.7759537160418355
7	0.6377337659003213	1.6259084163826127
8	0.49081499531927997	1.4301000994398239
9	0.411860559862125	1.3500546282920551
>10	5.067325015704451	40.28000597942073
>50	0.15708373008436727	3.610688610125562
>100	0.03927093215629572	2.7515216651467953
>500	0.003465082249084916	0.784846945256682
>1k	0.0030800731102977033	2.440499595363104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	2907	0.407851742314022	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2668	0.3743200717212971	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	2411	0.3382630033433461	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	2232	0.31314932536804163	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTA	2052	0.2878953475157803	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCG	1838	0.25787117384698055	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1680	0.23570379328777327	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	1616	0.22672460116252477	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25815177360089453	0.0
2	0.0	0.0	0.0	1.1745905698840702	0.0
3	0.0	0.0	0.0	1.526462661292246	0.0
4	0.0	0.0	0.0	2.0392587115701097	0.0
5	0.0	0.0	0.0	3.51114472072608	0.0
6	0.0	0.0	0.0	4.84750104874158	0.0
7	0.0	0.0	0.0	5.797892415248352	0.0
8	0.0	0.0	0.0	7.434209880197935	0.0
9	0.0	0.0	0.0	8.048863641146587	0.0
10	0.0	0.0	0.0	9.485113481555477	0.0
11	0.0	0.0	0.0	10.983235567702406	0.0
12	0.0	0.0	0.0	12.441091589162676	0.0
13	0.0	0.0	0.0	12.967075827874499	0.0
14	0.0	0.0	0.0	13.276437056564701	0.0
15	0.0	0.0	0.0	13.663384117212129	0.0
16	0.0	0.0	0.0	14.495783287198057	0.0
17	0.0	0.0	0.0	15.471288331680134	0.0
18	0.0	0.0	0.0	16.56632887132958	0.0
19	0.0	0.0	0.0	17.133420973989807	0.0
20	0.0	0.0	0.0	17.63344973546458	0.0
21	0.0	0.0	0.0	18.281354567252045	0.0
22	0.0	0.0	0.0	18.975698658312275	0.0
23	0.0	0.0	0.0	19.704837118857846	0.0
24	0.0	0.0	0.0	20.274594919180256	0.0
25	0.0	0.0	0.0	20.763119090744556	0.0
26	0.0	0.0	0.0	21.19622481091084	0.0
27	0.0	0.0	0.0	21.660617403638536	0.0
28	0.0	0.0	0.0	22.135532487138008	0.0
29	0.0	0.0	0.0	22.644540440738034	0.0
30	0.0	0.0	0.0	23.22608343072483	0.0
31	0.0	0.0	0.0	23.73453018481703	0.0
32	0.0	0.0	0.0	24.24929043337229	0.0
33	0.0	0.0	0.0	24.73795490481355	0.0
34	0.0	0.0	0.0	25.225216377485236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGAC	20	0.0012554476	40.000004	37
GGCGTAT	20	0.0012554476	40.000004	8
GGCGTAA	20	0.0012554476	40.000004	8
CGTTCAC	20	0.0012554476	40.000004	30
ATCGTTG	40	1.9264917E-8	40.000004	23
TATTGCG	20	0.0012554476	40.000004	1
CCGTACG	20	0.0012554476	40.000004	1
CTAAACG	20	0.0012554476	40.000004	1
CTACGCA	40	1.9264917E-8	40.000004	15
TCGTAAG	40	1.9264917E-8	40.000004	1
ACCGTCC	20	0.0012554476	40.000004	27
CAATCGT	20	0.0012554476	40.000004	26
TCGAACG	20	0.0012554476	40.000004	1
GGCTCGA	20	0.0012554476	40.000004	23
AGTCGAC	20	0.0012554476	40.000004	24
CTAGACG	30	4.864909E-6	40.0	1
GCGAATT	25	7.791671E-5	40.0	9
GGTATAC	30	4.864909E-6	40.0	8
CGTAAGG	85	0.0	40.0	2
TATAGCG	35	3.0541923E-7	40.0	1
>>END_MODULE