##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553714_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	649399
Sequences flagged as poor quality	0
Sequence length	46
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.648940019926115	31.0	31.0	34.0	30.0	34.0
2	31.813695432238116	31.0	31.0	34.0	30.0	34.0
3	31.725453842706873	31.0	31.0	34.0	30.0	34.0
4	35.52915849885817	37.0	35.0	37.0	33.0	37.0
5	33.637406278728484	37.0	35.0	37.0	30.0	37.0
6	34.457529192376334	37.0	35.0	37.0	30.0	37.0
7	35.38988048949875	37.0	35.0	37.0	32.0	37.0
8	35.434142953715664	37.0	35.0	37.0	33.0	37.0
9	37.2957303599174	39.0	37.0	39.0	34.0	39.0
10	36.60281583433297	38.0	35.0	39.0	32.0	39.0
11	36.323617683427294	38.0	35.0	39.0	32.0	39.0
12	36.49563519500338	38.0	35.0	39.0	32.0	39.0
13	32.80804251315447	38.0	34.0	39.0	8.0	39.0
14	35.0877565256491	39.0	34.0	40.0	17.0	41.0
15	36.49982984266992	39.0	34.0	40.0	30.0	41.0
16	37.17038061345952	39.0	36.0	41.0	32.0	41.0
17	37.35312342642967	39.0	36.0	40.0	32.0	41.0
18	37.45168378762517	39.0	36.0	40.0	32.0	41.0
19	37.43488979810563	39.0	36.0	40.0	32.0	41.0
20	37.378009513411634	39.0	36.0	41.0	32.0	41.0
21	37.35415514960756	39.0	35.0	40.0	32.0	41.0
22	37.355675016438276	39.0	35.0	40.0	32.0	41.0
23	37.224134930913046	39.0	35.0	40.0	32.0	41.0
24	37.21047152829	39.0	35.0	40.0	32.0	41.0
25	36.99920695904983	39.0	35.0	40.0	31.0	41.0
26	36.936120936435074	39.0	35.0	40.0	31.0	41.0
27	36.51597862023194	39.0	35.0	40.0	31.0	41.0
28	36.58498704186486	39.0	35.0	40.0	30.0	41.0
29	36.67178421894706	39.0	35.0	40.0	30.0	41.0
30	36.165060309609345	38.0	35.0	40.0	30.0	41.0
31	36.23046231977567	38.0	35.0	40.0	30.0	41.0
32	36.1535758447426	38.0	35.0	40.0	30.0	41.0
33	36.054139288788555	38.0	35.0	40.0	30.0	41.0
34	35.99844317592112	38.0	35.0	40.0	29.0	41.0
35	35.93892044798344	38.0	35.0	40.0	29.0	41.0
36	35.78469323174196	38.0	35.0	40.0	29.0	41.0
37	35.70151632509443	38.0	35.0	40.0	28.0	41.0
38	35.6057862731541	38.0	35.0	40.0	27.0	41.0
39	35.5227448764165	38.0	34.0	40.0	27.0	41.0
40	35.400642748140974	38.0	34.0	40.0	27.0	41.0
41	35.23151714123367	38.0	34.0	40.0	26.0	41.0
42	35.18960608193114	38.0	34.0	40.0	26.0	41.0
43	35.05684024767516	38.0	34.0	40.0	26.0	41.0
44	34.98820755806523	38.0	34.0	40.0	26.0	41.0
45	34.93067590187235	38.0	34.0	40.0	26.0	41.0
46	33.73893553885978	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	7.0
10	12.0
11	14.0
12	14.0
13	5.0
14	8.0
15	11.0
16	45.0
17	85.0
18	201.0
19	406.0
20	728.0
21	1229.0
22	1892.0
23	2679.0
24	3752.0
25	5135.0
26	6697.0
27	8852.0
28	10938.0
29	13340.0
30	16400.0
31	20758.0
32	27064.0
33	35794.0
34	49843.0
35	62368.0
36	70134.0
37	93091.0
38	114627.0
39	103262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.24196988292252	22.190671682586515	20.81909581012598	21.74826262436499
2	25.368379070494413	28.590281167664255	22.996339692546496	23.04500006929484
3	26.538537940464952	28.815258415858352	23.144168685199702	21.502034958476994
4	23.62245707184643	28.008050520558243	24.897174156412312	23.472318251183015
5	21.320636465408786	34.90180921128613	21.22069790683386	22.55685641647123
6	18.614287980117002	42.1210996629191	22.282910814460756	16.981701542503146
7	81.06064222457996	5.521566864131297	9.596411451203343	3.821379460085402
8	80.79901570529059	6.682332433527	7.915472613909167	4.603179247273248
9	75.55570612212215	7.004784423751807	10.240237511914863	7.199271942211183
10	35.608154616807234	25.223321871453454	20.03036653890751	19.1381569728318
11	28.868230471559087	28.339433845755845	23.239487587754216	19.55284809493085
12	23.163417251951422	25.31663892306579	29.911502789502293	21.608441035480496
13	20.828797087768844	31.77999966122523	29.526993420069942	17.86420983093599
14	21.828336662052145	29.827425049930785	28.242883034929218	20.101355253087856
15	20.34943078138402	27.717474156874278	32.46016701596399	19.472928045777714
16	21.326949995303348	26.091509226223014	29.97125034069963	22.610290437774
17	21.19051615416716	26.996191863553843	28.39702555747699	23.41626642480201
18	22.400096088845224	25.78568799767169	29.35437227344052	22.45984364004256
19	23.2023763510569	27.66973771133002	27.2710613967684	21.856824540844688
20	24.832806949194563	26.92812893151976	27.989109930874545	20.24995418841113
21	24.628926130160348	27.98510622899019	27.999273174119455	19.386694466730006
22	23.2986191848155	24.7973895863714	27.4781759750169	24.425815253796202
23	21.662029045317286	28.369923575490567	27.781379398489985	22.186667980702158
24	22.434589520464307	25.767209373590045	28.92197246992989	22.876228636015764
25	21.947677775912805	29.532537007294437	26.578112993706487	21.941672223086268
26	21.450140822514356	27.859143608166935	27.862531355915237	22.82818421340347
27	22.737330978335354	28.48141127411653	26.69776208463518	22.083495662912938
28	20.501571452989612	27.942297416534366	29.571034140797874	21.985096989678148
29	22.711460904621042	26.541309734077203	27.711160626979712	23.036068734322043
30	22.214539905358645	29.03053438640959	27.148486523693443	21.60643918453832
31	23.31956162544137	29.11137836676681	25.163420331722097	22.40563967606972
32	23.443830372390472	28.324650946490525	26.474940675917267	21.75657800520173
33	22.245183623627383	27.80555559833015	25.217008341558888	24.732252436483577
34	22.441826981562954	28.00851248616028	26.822646785720334	22.72701374655643
35	22.892859397689246	26.77290848923389	26.0286819043454	24.305550208731457
36	24.206227604292586	29.17220383770224	24.42211952897987	22.1994490290253
37	21.81955931561336	29.17743944785871	27.217011421329566	21.785989815198363
38	21.46061204282729	27.76752043042875	26.508356187798256	24.2635113389457
39	23.224550699954882	26.471860905236994	26.595667686584058	23.707920708224066
40	22.576720937359003	25.85667671185203	27.463701052819605	24.102901297969353
41	21.520513582558642	27.65079712164632	26.659418939665752	24.16927035612928
42	23.175890323206534	26.235334516991866	27.189755450809134	23.39901970899247
43	23.50896752227829	25.848823296617336	27.372847817751488	23.269361363352882
44	22.17280901264092	26.124616761035973	27.605370504112265	24.097203722210843
45	22.34866391848463	25.866224000960887	26.609064689043255	25.17604739151123
46	23.365758185645497	26.94753148680549	26.21824178971634	23.468468537832674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3460.0
1	3035.5
2	2611.0
3	4808.0
4	7005.0
5	4984.5
6	2964.0
7	2964.0
8	2788.0
9	2612.0
10	2595.0
11	2578.0
12	2626.0
13	2674.0
14	2542.5
15	2411.0
16	2346.5
17	2282.0
18	2153.0
19	2024.0
20	2024.0
21	2285.0
22	2546.0
23	2660.0
24	2774.0
25	3411.0
26	4048.0
27	4628.5
28	5209.0
29	7052.0
30	8895.0
31	8895.0
32	10280.5
33	11666.0
34	13687.5
35	15709.0
36	18205.0
37	20701.0
38	22653.5
39	24606.0
40	28581.0
41	32556.0
42	38326.0
43	44096.0
44	44096.0
45	47028.5
46	49961.0
47	58521.5
48	67082.0
49	64419.0
50	61756.0
51	58361.0
52	54966.0
53	49179.5
54	43393.0
55	39548.0
56	35703.0
57	35703.0
58	33209.0
59	30715.0
60	28755.5
61	26796.0
62	23705.5
63	20615.0
64	18277.5
65	15940.0
66	14003.5
67	12067.0
68	10433.5
69	8800.0
70	8800.0
71	7673.0
72	6546.0
73	5578.5
74	4611.0
75	3634.5
76	2658.0
77	2344.5
78	2031.0
79	1642.5
80	1254.0
81	1254.0
82	882.0
83	510.0
84	359.5
85	209.0
86	193.0
87	177.0
88	160.5
89	144.0
90	84.5
91	25.0
92	17.5
93	10.0
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	649399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.84022746131545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.48396852755964	28.94170766226949
2	9.027385568826771	6.831966466108032
3	3.4338531685374476	3.8981335489864737
4	1.8141587715333287	2.7459272226304705
5	1.1950247006185384	2.2610003246647947
6	0.8702111827721477	1.9757393457287054
7	0.6591710987412781	1.7460229018606634
8	0.5394675510521716	1.633085987185038
9	0.48054206887632006	1.6365439071909955
>10	5.383475573033446	40.796874280028305
>50	0.08214044593001515	1.974020081025021
>100	0.02536690235117974	1.870108244880923
>500	0.002415895462017118	0.7129083002931269
>1k	0.0028185447056866377	2.9759617271479706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	4150	0.639052416157093	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTA	3420	0.5266407863270501	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3394	0.5226370844426924	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3111	0.4790583293167991	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCG	2908	0.4477986569120063	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT	1522	0.23437054876893865	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT	1027	0.15814622443212878	No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGT	980	0.1509087633334822	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT	950	0.14628910731306946	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	810	0.12473071255114344	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATG	684	0.10532815726541002	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17523895170765585	0.0
2	0.0	0.0	0.0	0.7431486651503929	0.0
3	0.0	0.0	0.0	0.9755173629771527	0.0
4	0.0	0.0	0.0	1.3748096316748255	0.0
5	0.0	0.0	0.0	2.604254087240664	0.0
6	0.0	0.0	0.0	3.385437920292455	0.0
7	0.0	0.0	0.0	3.9878410653542735	0.0
8	0.0	0.0	0.0	4.923475398021863	0.0
9	0.0	0.0	0.0	5.253472826413345	0.0
10	0.0	0.0	0.0	6.499702032186684	0.0
11	1.5398853401375734E-4	0.0	0.0	7.438724112602576	0.0
12	1.5398853401375734E-4	0.0	0.0	8.701430091515386	0.0
13	1.5398853401375734E-4	0.0	0.0	9.097642589532784	0.0
14	1.5398853401375734E-4	0.0	0.0	9.332937069505805	0.0
15	3.079770680275147E-4	0.0	0.0	9.67001797046192	0.0
16	3.079770680275147E-4	0.0	0.0	10.224222704377432	0.0
17	3.079770680275147E-4	0.0	0.0	10.840792794568516	1.5398853401375734E-4
18	3.079770680275147E-4	0.0	0.0	11.540670681661043	1.5398853401375734E-4
19	3.079770680275147E-4	0.0	0.0	11.969682737423371	1.5398853401375734E-4
20	3.079770680275147E-4	0.0	0.0	12.312769191206023	1.5398853401375734E-4
21	3.079770680275147E-4	0.0	0.0	12.756872123301699	1.5398853401375734E-4
22	3.079770680275147E-4	0.0	0.0	13.202206963669486	1.5398853401375734E-4
23	3.079770680275147E-4	0.0	0.0	13.689580673823027	1.5398853401375734E-4
24	4.6196560204127203E-4	0.0	0.0	14.09580242655132	1.5398853401375734E-4
25	4.6196560204127203E-4	0.0	0.0	14.439812811538053	1.5398853401375734E-4
26	4.6196560204127203E-4	0.0	0.0	14.745480051555361	1.5398853401375734E-4
27	4.6196560204127203E-4	0.0	0.0	15.07578545701487	1.5398853401375734E-4
28	4.6196560204127203E-4	0.0	0.0	15.421335727341742	1.5398853401375734E-4
29	4.6196560204127203E-4	0.0	0.0	15.794911910859117	1.5398853401375734E-4
30	4.6196560204127203E-4	0.0	0.0	16.238706865886765	1.5398853401375734E-4
31	4.6196560204127203E-4	0.0	0.0	16.600579920819097	1.5398853401375734E-4
32	4.6196560204127203E-4	0.0	0.0	16.952135743972505	1.5398853401375734E-4
33	4.6196560204127203E-4	0.0	0.0	17.318474466391233	1.5398853401375734E-4
34	4.6196560204127203E-4	0.0	0.0	17.682965326401796	1.5398853401375734E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAC	20	0.0012553653	40.0	40
AATCCGG	25	7.790903E-5	40.0	2
GATCGTT	25	7.790903E-5	40.0	9
CGATTAC	25	7.790903E-5	40.0	10
ACGATAG	25	7.790903E-5	40.0	1
TTCGGTA	20	0.0012553653	40.0	25
GTATACG	20	0.0012553653	40.0	1
TAGGCCG	20	0.0012553653	40.0	1
GTAAGCG	20	0.0012553653	40.0	1
CGCCGGT	75	0.0	40.0	28
CGCCACG	20	0.0012553653	40.0	16
TACACGA	180	0.0	38.88889	9
CGTAAGG	155	0.0	37.419353	2
CGACGCT	170	0.0	36.47059	13
CGTTTTT	1830	0.0	36.393444	1
TAAGCGG	105	0.0	36.190475	2
TACGGGT	95	0.0	35.78947	4
TACGGCT	500	0.0	35.6	7
GGTATAC	45	5.4362317E-8	35.555557	8
CTCGACG	45	5.4362317E-8	35.555557	1
>>END_MODULE