##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	844643
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1682793795722	33.0	31.0	34.0	30.0	34.0
2	32.31113144843443	34.0	31.0	34.0	30.0	34.0
3	32.33970565078974	34.0	31.0	34.0	30.0	34.0
4	35.867309620751016	37.0	35.0	37.0	35.0	37.0
5	35.794380584459944	37.0	35.0	37.0	35.0	37.0
6	35.7712157680819	37.0	35.0	37.0	35.0	37.0
7	35.89314775591581	37.0	35.0	37.0	35.0	37.0
8	35.79632696890876	37.0	35.0	37.0	35.0	37.0
9	37.6107917783016	39.0	37.0	39.0	35.0	39.0
10	37.09098518545705	39.0	37.0	39.0	33.0	39.0
11	37.01314756648667	39.0	37.0	39.0	33.0	39.0
12	36.93891502090232	39.0	37.0	39.0	33.0	39.0
13	36.92328711656877	39.0	37.0	39.0	33.0	39.0
14	38.1008769385409	40.0	37.0	41.0	33.0	41.0
15	38.17872994862918	40.0	37.0	41.0	33.0	41.0
16	38.246539662318874	40.0	37.0	41.0	34.0	41.0
17	38.16692732906092	40.0	37.0	41.0	33.0	41.0
18	38.0940634090379	40.0	37.0	41.0	33.0	41.0
19	37.989163469063264	40.0	37.0	41.0	34.0	41.0
20	37.897876380908855	40.0	36.0	41.0	33.0	41.0
21	37.78746997252094	40.0	36.0	41.0	33.0	41.0
22	37.770254415178954	40.0	36.0	41.0	33.0	41.0
23	37.67577662988979	40.0	36.0	41.0	33.0	41.0
24	37.63634695368339	40.0	35.0	41.0	33.0	41.0
25	37.5518864182856	39.0	35.0	41.0	33.0	41.0
26	37.462684234641145	39.0	35.0	41.0	33.0	41.0
27	37.40116001671712	39.0	35.0	41.0	33.0	41.0
28	37.29308595465777	39.0	35.0	41.0	32.0	41.0
29	37.20557087432205	39.0	35.0	41.0	32.0	41.0
30	37.07085597110258	39.0	35.0	41.0	32.0	41.0
31	36.906950036879486	39.0	35.0	41.0	31.0	41.0
32	36.76975242794885	39.0	35.0	41.0	31.0	41.0
33	36.643804542274076	39.0	35.0	41.0	31.0	41.0
34	36.49785885871309	39.0	35.0	41.0	30.0	41.0
35	36.30721263302958	39.0	35.0	41.0	30.0	41.0
36	36.18182593119223	39.0	35.0	41.0	30.0	41.0
37	36.10052175889695	39.0	35.0	41.0	29.0	41.0
38	35.99114537147647	39.0	35.0	41.0	29.0	41.0
39	35.927471132774436	39.0	35.0	41.0	29.0	41.0
40	35.80656679804367	38.0	35.0	41.0	28.0	41.0
41	35.656006146975706	38.0	35.0	40.0	28.0	41.0
42	35.502559069334616	38.0	35.0	40.0	27.0	41.0
43	35.401531771411115	38.0	35.0	40.0	27.0	41.0
44	35.29204172650457	38.0	34.0	40.0	26.0	41.0
45	35.22714566982737	38.0	34.0	40.0	26.0	41.0
46	35.090503325073435	38.0	34.0	40.0	26.0	41.0
47	34.964222754465496	38.0	34.0	40.0	25.0	41.0
48	34.793838343536855	37.0	34.0	40.0	24.0	41.0
49	34.714782458387745	37.0	34.0	40.0	24.0	41.0
50	34.63677790498471	37.0	34.0	40.0	24.0	41.0
51	33.26858092708991	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	11.0
12	15.0
13	13.0
14	28.0
15	57.0
16	122.0
17	269.0
18	450.0
19	782.0
20	1272.0
21	1924.0
22	2858.0
23	4258.0
24	5675.0
25	7437.0
26	9089.0
27	10578.0
28	11866.0
29	13484.0
30	15791.0
31	19396.0
32	25074.0
33	33501.0
34	56184.0
35	69088.0
36	69841.0
37	98305.0
38	158741.0
39	228449.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63891016678052	20.35996272981603	21.19771311666586	14.803413986737594
2	30.177720054508235	27.058177241745923	25.24001264439533	17.52409005935052
3	31.137652238874885	26.57359381419132	26.275716486136748	16.013037460797047
4	27.685069313307515	28.456282713525123	25.991335984552055	17.86731198861531
5	24.236748543467478	32.62076403877141	24.489162877097186	18.653324540663927
6	24.392554013944352	37.1427928722549	24.072181975106645	14.392471138694098
7	80.94863747168922	7.668446905970925	7.698400389276889	3.684515233062963
8	82.77319530263082	6.2220370026153065	7.135914226483851	3.868853468270027
9	77.23629983318396	9.173343057362697	8.747719450702842	4.842637658750502
10	39.4487375139556	26.85690877684418	17.682500180549653	16.011853528650565
11	30.56320836140239	27.4205788717837	22.748545835341087	19.267666931472824
12	26.397306317580327	24.492004314248742	29.028713906348596	20.08197546182233
13	24.06910375152579	26.49213928251344	29.113246661607327	20.325510304353436
14	21.96466436115613	28.672823903116463	28.42242225413577	20.94008948159163
15	21.311252209513366	27.460832564764047	31.02079813601723	20.207117089705353
16	23.793247561395763	24.43115020191963	30.629745348034614	21.14585688865
17	23.083835419224453	25.69097239898987	27.907174983987314	23.318017197798362
18	23.88156889952323	25.942557980117044	28.412240437676033	21.76363268268369
19	25.246405877986323	27.668967836115378	26.150101285395134	20.93452500050317
20	25.298025319572886	26.634329533305788	26.879877060485907	21.18776808663542
21	24.90460466729731	27.401872743869305	27.018515514838814	20.675007073994575
22	23.440198995315182	26.000097082436014	26.16904419973882	24.390659722509984
23	23.167894601624592	27.775758515727944	26.60757266679532	22.44877421585214
24	22.755412641790674	27.31331461931254	28.019766931117644	21.911505807779143
25	22.985450657851896	28.2693398275958	26.378836976095226	22.366372538457078
26	21.00852075965822	29.135031013102576	26.45496381311394	23.401484414125257
27	22.52963678145678	28.365356724675394	27.256130696637516	21.84887579723031
28	21.184926649483867	28.172730964442966	28.659208683431935	21.983133702641233
29	22.622457061740878	28.07730603343661	26.93457472565332	22.36566217916919
30	23.32547597032119	27.523817754956823	27.127674058744343	22.02303221597764
31	24.398000101818166	27.849872668097646	25.449923813966375	22.302203416117816
32	23.725763429046353	28.290058640159216	25.926574896139552	22.05760303465488
33	24.218397595197025	27.57081986117212	24.73897256000464	23.47180998362622
34	22.510338687469144	27.674769103633135	26.220900427754685	23.59399178114304
35	23.1706176455615	27.182726903555704	25.67250305750477	23.97415239337803
36	23.868900825555887	29.01403314773224	25.411682805635046	21.705383221076836
37	22.89144644542132	28.006862070721	26.89467621231692	22.207015271540758
38	23.45760279786845	28.02118764969342	25.962329646963273	22.558879905474857
39	24.30802125868562	26.169517772597416	25.741407908429952	23.781053060287007
40	23.950592143663062	26.129974438904956	27.641974183175616	22.277459234256366
41	21.666550246672262	26.19142051730731	28.139936043985447	24.00209319203498
42	22.409467668588977	27.738938225972394	26.56945005167864	23.28214405375999
43	22.582321761975177	25.981746134165558	27.498126427378196	23.93780567648107
44	22.537332340408906	26.312181596248358	26.95138656213335	24.199099501209385
45	23.324292038174708	26.548139273041983	26.568858085605395	23.55871060317791
46	22.935607114485055	27.357001715517683	26.919893967036963	22.787497202960306
47	23.385738116577063	26.850397150038535	27.070134956425377	22.693729776959024
48	22.74274456782333	27.098667721155568	27.16496792135849	22.993619789662613
49	22.929924240181947	26.727623386448474	27.065517621054102	23.276934752315476
50	22.121653763779488	26.128790506758477	27.68649003188329	24.06306569757874
51	22.035345110301037	26.53712870407971	26.403462764742024	25.024063420877223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3820.0
1	4052.5
2	4285.0
3	5141.0
4	5997.0
5	4484.5
6	2972.0
7	3212.5
8	3453.0
9	3803.5
10	4154.0
11	4115.5
12	4077.0
13	4099.0
14	4121.0
15	3993.5
16	3866.0
17	3810.0
18	3754.0
19	3508.0
20	3262.0
21	3596.5
22	3931.0
23	4321.5
24	4712.0
25	5504.0
26	7479.0
27	8662.0
28	10551.5
29	12441.0
30	13904.5
31	15368.0
32	16384.0
33	17400.0
34	19472.0
35	21544.0
36	22758.0
37	23972.0
38	25902.0
39	27832.0
40	30872.0
41	33912.0
42	37434.5
43	40957.0
44	45246.5
45	49536.0
46	54217.0
47	58898.0
48	61937.0
49	64976.0
50	63931.0
51	62886.0
52	58012.5
53	53139.0
54	50206.5
55	47274.0
56	44582.0
57	41890.0
58	40277.5
59	38665.0
60	37458.0
61	36251.0
62	33187.0
63	30123.0
64	27149.0
65	24175.0
66	20990.0
67	17805.0
68	16150.0
69	14495.0
70	13949.5
71	13404.0
72	12186.5
73	10969.0
74	9420.0
75	6308.5
76	4746.0
77	3871.5
78	2997.0
79	2438.0
80	1879.0
81	1384.5
82	890.0
83	628.0
84	366.0
85	342.0
86	318.0
87	224.0
88	130.0
89	96.5
90	63.0
91	68.0
92	73.0
93	46.5
94	20.0
95	16.5
96	13.0
97	7.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	844643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.71252379786328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.07627499671334	20.32189306302496
2	8.50979620849354	4.546362674687008
3	3.094683064247877	2.4800038500169763
4	1.705067157896515	1.8218658812906303
5	1.0654898418801784	1.4230961378802924
6	0.7914779878796611	1.2685424752032233
7	0.6086037267395322	1.1380139073798625
8	0.53907386159155	1.1520018685256268
9	0.43876180584030033	1.054839166209224
>10	5.883002339205429	37.81820416314533
>50	1.1649123739795146	20.43747676093525
>100	0.11617964447099421	4.601550585660728
>500	0.004896460111804354	0.9387643575749103
>1k	0.0017805309497470377	0.9973851084659084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2940	0.3480760510653613	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2673	0.3164650627543234	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1392	0.16480335479013025	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	1383	0.16373781585829753	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	906	0.10726425247116238	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	885	0.10477799496355264	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	877	0.103830849246368	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.183932146480821E-4	0.0	0.0	0.08678222633704417	0.0
2	1.183932146480821E-4	0.0	0.0	0.3474840849921209	0.0
3	1.183932146480821E-4	0.0	0.0	0.48884558328193095	0.0
4	1.183932146480821E-4	0.0	0.0	0.6672641577565906	0.0
5	1.183932146480821E-4	0.0	0.0	1.1749342621675667	0.0
6	1.183932146480821E-4	0.0	0.0	1.6005578688274218	0.0
7	1.183932146480821E-4	0.0	0.0	1.9603548481429431	0.0
8	1.183932146480821E-4	0.0	0.0	2.554807178890963	0.0
9	1.183932146480821E-4	0.0	0.0	2.842384297271155	0.0
10	1.183932146480821E-4	0.0	0.0	3.456016328792164	0.0
11	1.183932146480821E-4	0.0	0.0	4.262629300189547	0.0
12	1.183932146480821E-4	0.0	0.0	4.876616511354501	0.0
13	1.183932146480821E-4	0.0	0.0	5.120032960670958	0.0
14	1.183932146480821E-4	0.0	0.0	5.230612223152266	0.0
15	1.183932146480821E-4	0.0	0.0	5.407254899407206	0.0
16	1.183932146480821E-4	0.0	0.0	5.777115301967814	0.0
17	1.183932146480821E-4	0.0	0.0	6.229377381923487	0.0
18	2.367864292961642E-4	0.0	0.0	6.764277925703522	0.0
19	2.367864292961642E-4	0.0	0.0	7.0401341158335535	0.0
20	3.5517964394424624E-4	0.0	0.0	7.313030475597382	0.0
21	7.103592878884925E-4	0.0	0.0	7.631034650142131	0.0
22	7.103592878884925E-4	0.0	0.0	7.985267148369193	0.0
23	7.103592878884925E-4	0.0	0.0	8.335947850156812	0.0
24	9.471457171846568E-4	0.0	0.0	8.621157104244041	0.0
25	9.471457171846568E-4	0.0	0.0	8.872032326083328	0.0
26	9.471457171846568E-4	0.0	0.0	9.09532192890961	0.0
27	0.0010655389318327388	0.0	0.0	9.337080873220994	0.0
28	0.0010655389318327388	0.0	0.0	9.600860955456922	0.0
29	0.0010655389318327388	0.0	0.0	9.853393682301279	0.0
30	0.0010655389318327388	0.0	0.0	10.134459173875827	0.0
31	0.0010655389318327388	0.0	0.0	10.41268322829882	0.0
32	0.0010655389318327388	0.0	0.0	10.69102567593646	0.0
33	0.0010655389318327388	0.0	0.0	10.970433662505934	0.0
34	0.0010655389318327388	0.0	0.0	11.237410361537359	0.0
35	0.0010655389318327388	0.0	0.0	11.535169296377285	0.0
36	0.0010655389318327388	0.0	0.0	11.802501175052655	0.0
37	0.001183932146480821	0.0	0.0	12.072792884094227	0.0
38	0.001183932146480821	0.0	0.0	12.347938714936369	0.0
39	0.001183932146480821	0.0	0.0	12.619769535768366	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	30	2.1648157E-6	45.000004	1
TATCGAG	30	2.1648157E-6	45.000004	1
CATTGCG	20	7.032223E-4	45.0	1
TAGAACG	40	6.8121153E-9	45.0	1
ATTACGG	20	7.032223E-4	45.0	2
CGATTGA	20	7.032223E-4	45.0	10
CCGTATG	20	7.032223E-4	45.0	1
CAATGCG	20	7.032223E-4	45.0	1
TATCGTC	40	6.8121153E-9	45.0	12
CGTTTAG	20	7.032223E-4	45.0	38
GTTAGCG	85	0.0	42.35294	1
TAGTTCG	45	1.9272193E-8	40.000004	1
TATTGCG	40	3.456771E-7	39.375	1
CGGTAGT	75	0.0	39.000004	12
CGAATAT	110	0.0	38.863636	14
TATAGCG	35	6.246324E-6	38.571426	1
GTGCGTA	35	6.246324E-6	38.571426	14
TGCGTAT	35	6.246324E-6	38.571426	15
CGTATGG	100	0.0	38.25	2
TACGGGA	250	0.0	37.800003	4
>>END_MODULE