##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553710_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1034558
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16987931077813	33.0	31.0	34.0	30.0	34.0
2	32.330092657927345	34.0	31.0	34.0	30.0	34.0
3	32.35355871782926	34.0	31.0	34.0	30.0	34.0
4	35.88335115092629	37.0	35.0	37.0	35.0	37.0
5	35.795643163553905	37.0	35.0	37.0	35.0	37.0
6	35.76632339607833	37.0	35.0	37.0	35.0	37.0
7	35.88454103104901	37.0	35.0	37.0	35.0	37.0
8	35.77537460442044	37.0	35.0	37.0	35.0	37.0
9	37.59065127329739	39.0	37.0	39.0	35.0	39.0
10	37.09127182816236	39.0	37.0	39.0	33.0	39.0
11	36.99806487408149	39.0	37.0	39.0	33.0	39.0
12	36.904485780400904	39.0	37.0	39.0	33.0	39.0
13	36.8728171837635	39.0	37.0	39.0	33.0	39.0
14	38.057765731839105	40.0	37.0	41.0	33.0	41.0
15	38.151779793882994	40.0	37.0	41.0	33.0	41.0
16	38.20754177146182	40.0	37.0	41.0	33.0	41.0
17	38.12448117940222	40.0	37.0	41.0	33.0	41.0
18	38.07790186727085	40.0	37.0	41.0	33.0	41.0
19	38.02239217134274	40.0	37.0	41.0	33.0	41.0
20	37.92769665886301	40.0	36.0	41.0	33.0	41.0
21	37.82610158154497	40.0	36.0	41.0	33.0	41.0
22	37.79896825504225	40.0	36.0	41.0	33.0	41.0
23	37.70316598972701	40.0	36.0	41.0	33.0	41.0
24	37.637612390992096	40.0	36.0	41.0	33.0	41.0
25	37.55147125632396	40.0	35.0	41.0	33.0	41.0
26	37.464548145198236	40.0	35.0	41.0	33.0	41.0
27	37.41524399791989	40.0	35.0	41.0	33.0	41.0
28	37.324161622644645	39.0	35.0	41.0	32.0	41.0
29	37.232014058177505	39.0	35.0	41.0	32.0	41.0
30	37.11445177554086	39.0	35.0	41.0	32.0	41.0
31	37.00849831522254	39.0	35.0	41.0	31.0	41.0
32	36.89014052377924	39.0	35.0	41.0	31.0	41.0
33	36.7715024193907	39.0	35.0	41.0	31.0	41.0
34	36.650273836749605	39.0	35.0	41.0	31.0	41.0
35	36.495396101523546	39.0	35.0	41.0	30.0	41.0
36	36.37000728813658	39.0	35.0	41.0	30.0	41.0
37	36.27730876374258	39.0	35.0	41.0	30.0	41.0
38	36.15998619700394	39.0	35.0	41.0	30.0	41.0
39	36.073772567608586	39.0	35.0	41.0	29.0	41.0
40	35.93899230395976	38.0	35.0	40.0	29.0	41.0
41	35.80869414764566	38.0	35.0	40.0	28.0	41.0
42	35.698624920014154	38.0	35.0	40.0	28.0	41.0
43	35.61585044047796	38.0	35.0	40.0	28.0	41.0
44	35.50519545545054	38.0	34.0	40.0	28.0	41.0
45	35.42434643586923	38.0	34.0	40.0	27.0	41.0
46	35.27139416059805	38.0	34.0	40.0	27.0	41.0
47	35.1855971342351	37.0	34.0	40.0	26.0	41.0
48	35.017200582277646	37.0	34.0	40.0	26.0	41.0
49	34.91534355734526	37.0	34.0	40.0	26.0	41.0
50	34.82897720572457	37.0	34.0	40.0	26.0	41.0
51	33.4632964029083	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	12.0
11	6.0
12	7.0
13	22.0
14	36.0
15	80.0
16	155.0
17	260.0
18	479.0
19	877.0
20	1441.0
21	2251.0
22	3359.0
23	4696.0
24	6220.0
25	8007.0
26	9775.0
27	11723.0
28	13636.0
29	15945.0
30	19441.0
31	23576.0
32	30545.0
33	41775.0
34	69801.0
35	83984.0
36	87117.0
37	124866.0
38	196471.0
39	277868.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88981381420858	19.0664032369379	24.416224126631857	13.627558822221664
2	31.45053249793632	25.447389126564197	24.861148432470678	18.24092994302881
3	31.40084944488371	25.53573603413245	26.328151732430662	16.73526278855318
4	27.980644874429466	26.534036757726486	26.352219981866654	19.133098385977394
5	24.631485136647726	31.41824817941575	24.105173417053464	19.845093266883055
6	24.524386259639382	37.00063215402133	23.881599678316732	14.593381908022558
7	81.42926737795271	6.975925950985832	7.637561161384862	3.9572455096765964
8	82.93106814697677	6.033397837530617	7.253629086044475	3.7819049294481313
9	77.95338685699593	7.800625967804608	8.561723943945143	5.684263231254313
10	40.71680853079286	25.789080940846237	16.98261479781704	16.511495730543864
11	30.998648698284676	27.391891029792433	22.397777601642442	19.211682670280446
12	26.760896924097054	23.410770589952424	28.789879349441982	21.03845313650854
13	24.284283723097207	25.39084323933506	29.32547039412	20.999402643447734
14	21.85600034024192	27.080163702760018	28.5178791329244	22.545956824073663
15	21.497973047427017	26.57241063333327	29.98845883942708	21.941157479812635
16	23.854534980155776	23.64961655122284	29.76401516396374	22.73183330465764
17	23.4616135586405	24.65313689517649	27.69569226664914	24.18955727953387
18	24.038381608377684	24.668119138801302	28.170774379010165	23.122724873810846
19	24.9817796585595	27.048749320966053	25.38195055279646	22.587520467677987
20	26.170403206006814	25.40070252223655	26.49885265011725	21.930041621639386
21	25.39074657969877	26.890807475269636	25.969641141434312	21.748804803597285
22	24.267271627110322	24.17283516245585	26.16122054055935	25.39867266987448
23	24.032485370564046	27.338148272015683	25.63684201369087	22.9925243437294
24	22.887648638355703	26.93942727232306	27.628707138700783	22.54421695062046
25	24.234310691135732	27.00186939736583	25.634038884238485	23.129781027259952
26	21.745615035599744	26.980700937018515	26.92618490215145	24.34749912523029
27	23.22760057918454	26.136379014033047	26.7666965022744	23.86932390450801
28	21.759340703952798	27.036086908612177	28.1651681201054	23.039404267329623
29	23.214938166830663	26.522437601371795	26.378318083664716	23.884306148132826
30	23.567262541104512	26.003182035226637	27.05667541114176	23.37288001252709
31	24.802186054334317	27.070304419858527	24.33754318269251	23.789966343114642
32	23.575961908370534	27.136902909261735	25.89183013422157	23.395305048146163
33	23.916977105198548	27.28227900224057	24.218071872239157	24.582672020321723
34	22.511739312827313	27.04546289333222	25.708756783089974	24.734041010750484
35	23.5779917607326	25.443522741112627	26.09752184024482	24.88096365790995
36	24.385196383383047	27.819319941462922	25.055047662866652	22.740436012287375
37	23.83665294744229	27.58917334745853	25.88989694149579	22.684276763603396
38	23.452044254647877	28.386905325752643	25.812375913191914	22.34867450640757
39	24.453728065512035	26.278082040832896	25.748097255059648	23.52009263859542
40	23.870967118324927	25.485086384716954	27.79689490584385	22.847051591114273
41	22.33272566641986	26.061177817000107	26.616390767844816	24.989705748735208
42	22.189766064348255	26.771916122634014	26.845570765486325	24.19274704753141
43	23.21223169701457	25.570436843560245	26.709280678318663	24.508050781106522
44	22.874696247092963	25.65917038967366	27.41508934250182	24.05104402073156
45	24.321691002341097	25.63490882096509	26.21631653324415	23.82708364344967
46	23.20691541701867	26.307949868446233	26.817636130598764	23.66749858393633
47	23.587657724361517	25.852779641160765	27.614691491438858	22.944871143038863
48	23.15732902360235	25.871048312419408	27.596422820180212	23.37519984379803
49	23.604959799257266	26.567577651518814	26.525337390460468	23.302125158763452
50	22.363173451850933	25.426413985489454	28.306194529451222	23.904218033208384
51	22.79659526097135	25.805416419379096	26.328345051703238	25.06964326794631
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3504.0
1	3611.5
2	3719.0
3	4711.0
4	5703.0
5	4332.5
6	2962.0
7	3389.5
8	3817.0
9	4412.0
10	5007.0
11	4265.0
12	3523.0
13	3498.5
14	3474.0
15	3248.5
16	3023.0
17	3044.0
18	3065.0
19	3045.0
20	3025.0
21	3515.5
22	4006.0
23	4289.5
24	4573.0
25	5908.5
26	8503.5
27	9763.0
28	11166.5
29	12570.0
30	15344.5
31	18119.0
32	20395.5
33	22672.0
34	24771.5
35	26871.0
36	28232.0
37	29593.0
38	31496.5
39	33400.0
40	36153.5
41	38907.0
42	43602.5
43	48298.0
44	52363.0
45	56428.0
46	63397.5
47	70367.0
48	72947.0
49	75527.0
50	75186.5
51	74846.0
52	70382.5
53	65919.0
54	63190.5
55	60462.0
56	57255.5
57	54049.0
58	53443.0
59	52837.0
60	50907.5
61	48978.0
62	44602.5
63	40227.0
64	36178.5
65	32130.0
66	29408.5
67	26687.0
68	23417.0
69	20147.0
70	19064.0
71	17981.0
72	16172.0
73	14363.0
74	12569.0
75	8772.0
76	6769.0
77	5291.5
78	3814.0
79	3234.5
80	2655.0
81	2099.5
82	1544.0
83	1052.5
84	561.0
85	410.0
86	259.0
87	207.5
88	156.0
89	147.5
90	139.0
91	90.5
92	42.0
93	43.0
94	44.0
95	26.0
96	8.0
97	5.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1034558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.54212017815361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1662897636592	17.69573826961982
2	8.542494194781607	4.022168499094562
3	3.036934188286669	2.144876089013644
4	1.5767049077143471	1.48475905691583
5	1.042714048173931	1.2273849716779865
6	0.8114541058838092	1.146201004786369
7	0.5971893967682691	0.9841373183486354
8	0.4937199751135296	0.9298571990782178
9	0.4490285029023194	0.9513974680868519
>10	6.5324578939959	38.17846094446744
>50	1.6191185355455096	24.862702665791186
>100	0.12362524033012903	4.438993099689917
>500	0.005375010449136045	0.9076749990046592
>1k	0.0028942363956886395	1.0256484144248894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2612	0.25247496998718294	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2043	0.19747563693867332	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1512	0.1461493700691503	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG	1144	0.11057862391475393	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1111	0.10738885591721295	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC	1065	0.1029425126479134	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC	1050	0.1014926181035766	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9331927257824113E-4	0.0	0.0	0.04330351705752602	0.0
2	1.9331927257824113E-4	0.0	0.0	0.2038551729337553	0.0
3	1.9331927257824113E-4	0.0	0.0	0.3035112579478386	0.0
4	1.9331927257824113E-4	0.0	0.0	0.4657061276409829	0.0
5	1.9331927257824113E-4	0.0	0.0	1.011639753401936	0.0
6	1.9331927257824113E-4	0.0	0.0	1.41345386145581	0.0
7	1.9331927257824113E-4	0.0	0.0	1.7663581935473893	0.0
8	1.9331927257824113E-4	0.0	0.0	2.312291819308342	0.0
9	1.9331927257824113E-4	0.0	0.0	2.5706630271091617	0.0
10	1.9331927257824113E-4	0.0	0.0	3.180005374275778	0.0
11	1.9331927257824113E-4	0.0	0.0	3.8444437141271925	0.0
12	1.9331927257824113E-4	0.0	0.0	4.403233071514599	0.0
13	1.9331927257824113E-4	0.0	0.0	4.613661099716014	0.0
14	1.9331927257824113E-4	0.0	0.0	4.70200800728427	0.0
15	1.9331927257824113E-4	0.0	0.0	4.8563734464379955	0.0
16	1.9331927257824113E-4	0.0	0.0	5.155728340025402	0.0
17	1.9331927257824113E-4	0.0	0.0	5.501769837940453	0.0
18	1.9331927257824113E-4	0.0	0.0	5.9007808165419435	0.0
19	1.9331927257824113E-4	0.0	0.0	6.134117178543881	0.0
20	2.899789088673617E-4	0.0	0.0	6.348894890378307	0.0
21	2.899789088673617E-4	0.0	0.0	6.616448763626592	0.0
22	2.899789088673617E-4	0.0	0.0	6.8833260194208545	0.0
23	2.899789088673617E-4	0.0	0.0	7.16953520247294	0.0
24	2.899789088673617E-4	0.0	0.0	7.386826064850883	0.0
25	2.899789088673617E-4	0.0	0.0	7.58062863561057	0.0
26	2.899789088673617E-4	0.0	0.0	7.770468161282403	0.0
27	2.899789088673617E-4	0.0	0.0	7.966590563313028	0.0
28	2.899789088673617E-4	0.0	0.0	8.156913387166307	0.0
29	3.8663854515648226E-4	0.0	0.0	8.371111141182999	0.0
30	4.8329818144560285E-4	0.0	0.0	8.603577566458332	0.0
31	4.8329818144560285E-4	0.0	0.0	8.81390893502346	0.0
32	4.8329818144560285E-4	0.0	0.0	9.043862209755277	0.0
33	4.8329818144560285E-4	0.0	0.0	9.272268930306469	0.0
34	4.8329818144560285E-4	0.0	0.0	9.490429729411014	0.0
35	4.8329818144560285E-4	0.0	0.0	9.730338946680611	0.0
36	4.8329818144560285E-4	0.0	0.0	9.95236613123672	0.0
37	5.799578177347234E-4	0.0	0.0	10.18222274633225	0.0
38	5.799578177347234E-4	0.0	0.0	10.42290524069216	0.0
39	5.799578177347234E-4	0.0	0.0	10.641549337978152	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGT	20	7.032958E-4	45.000004	8
ATTACCG	20	7.032958E-4	45.000004	42
CCGTCTA	20	7.032958E-4	45.000004	21
TTACGTC	20	7.032958E-4	45.000004	9
TACGTCG	20	7.032958E-4	45.000004	26
TAATGCG	55	1.8189894E-12	45.0	1
AATACGC	25	3.8905353E-5	45.0	27
GACGTAG	60	3.6379788E-12	41.250004	1
GACACGA	330	0.0	40.909092	25
ACGCATT	50	1.0804797E-9	40.5	17
TAACGGG	235	0.0	40.212765	3
TCGACAG	45	1.9279469E-8	40.0	1
AACACGT	345	0.0	39.782608	41
CACGACC	340	0.0	39.705883	27
CGAGACA	365	0.0	39.452053	22
CGACGGT	110	0.0	38.863636	28
GATCGTA	35	6.247683E-6	38.57143	10
ATAACGC	70	0.0	38.57143	11
GCGATAA	100	0.0	38.25	9
TAAACGG	65	9.094947E-12	38.076927	2
>>END_MODULE