FastQCFastQC Report
Sat 18 Jun 2016
SRR3553709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813271
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.252560339665376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17880.21985291495700698No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC8850.10881981528912256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG502.1827873E-1145.01
TATTGCG351.2113378E-745.01
TCGTATA207.0320687E-445.029
ATAGTCG253.889796E-545.014
TATACGG351.2113378E-745.02
TGTACCG253.889796E-545.01
TATCGCG207.0320687E-445.032
ATACCGA253.889796E-545.014
TACGTGA253.889796E-545.024
CGTAAGG900.042.52
CATACGA2550.040.58823418
ACATACG2600.039.80769317
ACGACCA4300.039.7674428
GCGAGAC4350.039.31034521
TAACGCC750.039.00000412
CATCGTT750.039.00000436
ACACGTG4400.038.86363642
CGGCACG356.2460367E-638.57142622
CGAATAT700.038.57142614
GGACGAT356.2460367E-638.5714268
Produced by FastQC (version 0.11.5)