##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813271
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16426627778441	33.0	31.0	34.0	30.0	34.0
2	32.32456585812109	34.0	31.0	34.0	30.0	34.0
3	32.34406120469069	34.0	31.0	34.0	30.0	34.0
4	35.88086382030098	37.0	35.0	37.0	35.0	37.0
5	35.78596925256157	37.0	35.0	37.0	35.0	37.0
6	35.74894715291705	37.0	35.0	37.0	35.0	37.0
7	35.85854284734117	37.0	35.0	37.0	35.0	37.0
8	35.77297850286067	37.0	35.0	37.0	35.0	37.0
9	37.54332688611791	39.0	37.0	39.0	35.0	39.0
10	37.062129351716706	39.0	37.0	39.0	33.0	39.0
11	36.97258109535444	39.0	37.0	39.0	33.0	39.0
12	36.89823072505967	39.0	37.0	39.0	33.0	39.0
13	36.85821208428679	39.0	37.0	39.0	33.0	39.0
14	38.02511954809651	40.0	37.0	41.0	33.0	41.0
15	38.123372160079484	40.0	37.0	41.0	33.0	41.0
16	38.18416370434947	40.0	37.0	41.0	33.0	41.0
17	38.11890501444168	40.0	37.0	41.0	33.0	41.0
18	38.06292121568333	40.0	37.0	41.0	33.0	41.0
19	37.980039863710864	40.0	37.0	41.0	33.0	41.0
20	37.90642725487568	40.0	36.0	41.0	33.0	41.0
21	37.80562198824254	40.0	36.0	41.0	33.0	41.0
22	37.74137157232952	40.0	36.0	41.0	33.0	41.0
23	37.632002124753	40.0	36.0	41.0	33.0	41.0
24	37.5840464002774	40.0	36.0	41.0	33.0	41.0
25	37.495468300234485	39.0	35.0	41.0	33.0	41.0
26	37.43086867723059	39.0	35.0	41.0	33.0	41.0
27	37.34816684721329	39.0	35.0	41.0	32.0	41.0
28	37.23690995990266	39.0	35.0	41.0	32.0	41.0
29	37.11721799990409	39.0	35.0	41.0	32.0	41.0
30	36.99791582387667	39.0	35.0	41.0	31.0	41.0
31	36.88927921935001	39.0	35.0	41.0	31.0	41.0
32	36.761026767215355	39.0	35.0	41.0	31.0	41.0
33	36.604320085186856	39.0	35.0	41.0	30.0	41.0
34	36.485493765300866	39.0	35.0	41.0	30.0	41.0
35	36.32054259896148	39.0	35.0	41.0	30.0	41.0
36	36.20555263866534	39.0	35.0	41.0	30.0	41.0
37	36.12383694979902	39.0	35.0	41.0	29.0	41.0
38	35.98584358719295	39.0	35.0	41.0	29.0	41.0
39	35.92093410437603	39.0	35.0	41.0	29.0	41.0
40	35.75721131086686	38.0	35.0	40.0	28.0	41.0
41	35.610118890259216	38.0	35.0	40.0	27.0	41.0
42	35.49679012284958	38.0	34.0	40.0	27.0	41.0
43	35.415276088782214	38.0	34.0	40.0	27.0	41.0
44	35.31278872602122	38.0	34.0	40.0	26.0	41.0
45	35.226465716839776	38.0	34.0	40.0	26.0	41.0
46	35.07713910861201	38.0	34.0	40.0	26.0	41.0
47	34.97149781561128	37.0	34.0	40.0	26.0	41.0
48	34.81473949028061	37.0	34.0	40.0	25.0	41.0
49	34.74080349600564	37.0	34.0	40.0	24.0	41.0
50	34.66490751545303	37.0	34.0	40.0	24.0	41.0
51	33.1999087634995	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	7.0
12	15.0
13	20.0
14	33.0
15	70.0
16	120.0
17	227.0
18	434.0
19	779.0
20	1294.0
21	1950.0
22	2886.0
23	3990.0
24	5363.0
25	6849.0
26	8402.0
27	9810.0
28	11395.0
29	13097.0
30	15848.0
31	19292.0
32	24991.0
33	33132.0
34	54048.0
35	65495.0
36	68711.0
37	97611.0
38	153363.0
39	213952.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32549789676504	19.81848608889288	24.2492354946875	13.60678051965458
2	32.60844171254109	25.14647638978889	24.458390868480496	17.786691029189534
3	32.11783034191555	25.702625570074428	25.369034430097713	16.810509657912306
4	28.407750921894426	26.949196516290385	25.198242652203263	19.444809909611926
5	24.82505831389537	30.968889828851637	24.470563932563685	19.73548792468931
6	25.37346099885524	36.428201669554184	23.829449224182344	14.368888107408232
7	82.00759648382888	6.721252817326574	7.502542203029494	3.7686084958150485
8	83.86491095833985	5.340040404735936	7.0757471986582585	3.719301438265966
9	78.10127251555754	8.193824690662769	8.40543926932105	5.299463524458637
10	39.73423373020801	26.43682118260703	16.49167374712734	17.33727134005762
11	31.179028884590743	28.19908738907449	21.7532655166605	18.868618209674263
12	27.19536292330601	24.17705783189121	28.132442937225104	20.49513630757767
13	25.106391350484646	25.524210257097575	28.435171056142416	20.934227336275363
14	21.762364574662072	28.000383635958986	28.84844043375455	21.388811355624384
15	21.526403867837413	26.837425655162917	30.234079415102716	21.402091061896957
16	23.76415733500887	23.927079657334392	30.929788471493513	21.378974536163224
17	22.856341858986735	24.789030962618856	28.435171056142416	23.919456122251994
18	23.97638671488348	25.299930773383046	27.67699819617323	23.04668431556025
19	25.29341388049002	27.210487033227547	25.847595696883324	21.648503389399107
20	26.546378759355736	25.541547651397874	26.124871045444877	21.787202543801513
21	27.007356711354518	25.929856099627308	25.79558351398242	21.267203675035752
22	25.020565100685992	24.612705973777498	25.75242446859657	24.614304456939937
23	24.148162174724046	27.63125698567882	25.980023878879244	22.240556960717893
24	22.921879668646735	26.220902995434486	27.7561845928356	23.10103274308318
25	24.225750088223975	26.854517128976692	26.098557553386264	22.821175229413075
26	23.07103044372663	27.128718471456626	26.23319901976094	23.567052065055805
27	23.96273812788111	27.42480673723765	25.75082598543413	22.86162914944711
28	22.677557665280084	27.932140700947162	26.949319476533653	22.4409821572391
29	23.144437708955564	27.739339039508355	25.80935506122805	23.306868190308027
30	24.321167236997262	26.622245229449966	26.479242466533297	22.57734506701948
31	24.34514448443385	27.75729123502498	24.27186017944818	23.625704101092992
32	24.484335479809314	27.53842200201409	25.137623252273844	22.839619265902755
33	24.07389418779226	27.429110345751905	23.809160784043694	24.687834682412134
34	22.551031574960867	28.351435130479263	25.46568118130365	23.631852113256222
35	23.31695093025572	27.383860976230558	24.431831455935352	24.86735663757837
36	23.781986570282232	28.71343008665008	24.731977409744108	22.77260593332358
37	24.213945904870577	27.407100462207556	24.862069347117995	23.516884285803872
38	23.558690768513816	28.06112599613167	25.40026633188691	22.9799169034676
39	24.230914418441085	26.66454355313296	25.102702543186712	24.001839485239238
40	23.290268557467314	27.205322703010438	27.180484733870998	22.323924005651254
41	21.669529590997342	27.94480560600341	26.883412786143857	23.50225201685539
42	23.141855543847008	27.895867429184122	25.995885750260367	22.966391276708503
43	22.641530314003575	26.52313927337874	26.744959552227975	24.090370860389708
44	23.217599053697967	26.81516985113203	26.542689952057803	23.424541143112197
45	23.549714670755503	26.497809463266243	24.92121322412824	25.03126264185001
46	23.372160079481503	27.57334271110122	25.459656129383685	23.594841080033593
47	23.174194087825583	27.13265319924109	27.076460368069196	22.616692344864134
48	22.682721995497197	27.281558053834452	26.607981841231275	23.427738109437076
49	22.96344023087015	27.359760768550707	26.522401511919153	23.154397488659992
50	21.74539606109157	26.898782816551925	26.942064822181045	24.413756300175464
51	22.658621787817346	26.593595492769325	25.980023878879244	24.76775884053409
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2605.0
1	2733.5
2	2862.0
3	3753.5
4	4645.0
5	3549.5
6	2454.0
7	2717.0
8	2980.0
9	3523.0
10	4066.0
11	3665.5
12	3265.0
13	3207.0
14	3149.0
15	3145.5
16	3142.0
17	3053.0
18	2964.0
19	3047.0
20	3130.0
21	3259.5
22	3389.0
23	3454.5
24	3520.0
25	4711.0
26	6833.0
27	7764.0
28	8931.5
29	10099.0
30	11828.5
31	13558.0
32	15568.5
33	17579.0
34	19021.5
35	20464.0
36	21767.0
37	23070.0
38	24820.0
39	26570.0
40	28650.0
41	30730.0
42	34663.0
43	38596.0
44	42921.0
45	47246.0
46	50415.0
47	53584.0
48	55130.0
49	56676.0
50	57273.5
51	57871.0
52	54538.5
53	51206.0
54	49297.5
55	47389.0
56	45421.5
57	43454.0
58	41667.0
59	39880.0
60	39529.0
61	39178.0
62	36541.0
63	33904.0
64	29543.0
65	25182.0
66	21946.0
67	18710.0
68	17059.5
69	15409.0
70	13855.5
71	12302.0
72	11864.0
73	11426.0
74	10116.5
75	7751.5
76	6696.0
77	5182.0
78	3668.0
79	2960.5
80	2253.0
81	1687.5
82	1122.0
83	775.0
84	428.0
85	305.0
86	182.0
87	121.5
88	61.0
89	65.5
90	70.0
91	42.0
92	14.0
93	13.0
94	12.0
95	20.0
96	28.0
97	16.5
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	813271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.32027217764047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3256802777352	19.072667265981803
2	7.909857138549829	4.0055947126866815
3	2.7935170974405876	2.1219783972026356
4	1.5968158975627484	1.617272525754882
5	1.070564780338948	1.355349581098902
6	0.8335754671150719	1.2663814624774445
7	0.6785899079783128	1.2027456816907616
8	0.6030459755628756	1.2215430589506189
9	0.5076384025830134	1.156818826910207
>10	7.600849179132963	47.156337512909296
>50	1.0168899727439074	16.174966374939416
>100	0.058094050290859914	2.5400587217691784
>500	0.0039054823724947846	0.6333768499851705
>1k	9.763705931236962E-4	0.4749090276430572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2054	0.252560339665376	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1788	0.21985291495700698	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC	885	0.10881981528912256	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.688807297936358E-4	0.0	0.0	0.035043669330395405	0.0
2	4.918409730581811E-4	0.0	0.0	0.16550448743407795	0.0
3	4.918409730581811E-4	0.0	0.0	0.25624914696331236	0.0
4	4.918409730581811E-4	0.0	0.0	0.3992519098799785	0.0
5	4.918409730581811E-4	0.0	0.0	0.8478108773090397	0.0
6	4.918409730581811E-4	0.0	0.0	1.240668854539262	0.0
7	4.918409730581811E-4	0.0	0.0	1.5655298172441905	0.0
8	4.918409730581811E-4	0.0	0.0	2.0557723071399323	0.0
9	4.918409730581811E-4	0.0	0.0	2.30513568048043	0.0
10	4.918409730581811E-4	0.0	0.0	2.8246427082731342	0.0
11	4.918409730581811E-4	0.0	0.0	3.498218920876313	0.0
12	4.918409730581811E-4	0.0	0.0	3.9515733377926914	0.0
13	4.918409730581811E-4	0.0	0.0	4.148063806529435	0.0
14	4.918409730581811E-4	0.0	0.0	4.224668038083246	0.0
15	4.918409730581811E-4	0.0	0.0	4.352915571808167	0.0
16	4.918409730581811E-4	0.0	0.0	4.6262561925852514	0.0
17	4.918409730581811E-4	0.0	0.0	4.920991895690366	0.0
18	4.918409730581811E-4	0.0	0.0	5.292208870106029	0.0
19	4.918409730581811E-4	0.0	0.0	5.493494788330089	0.0
20	4.918409730581811E-4	0.0	0.0	5.693059263148446	0.0
21	4.918409730581811E-4	0.0	0.0	5.932954697757574	1.2296024326454528E-4
22	4.918409730581811E-4	0.0	0.0	6.191786009829442	1.2296024326454528E-4
23	4.918409730581811E-4	0.0	0.0	6.45467500992904	1.2296024326454528E-4
24	6.148012163227263E-4	0.0	0.0	6.660264536667359	1.2296024326454528E-4
25	6.148012163227263E-4	0.0	0.0	6.84261457742868	1.2296024326454528E-4
26	7.377614595872716E-4	0.0	0.0	7.000495529780356	1.2296024326454528E-4
27	7.377614595872716E-4	0.0	0.0	7.180263405433121	1.2296024326454528E-4
28	7.377614595872716E-4	0.0	0.0	7.361137923275267	1.2296024326454528E-4
29	8.60721702851817E-4	0.0	0.0	7.557628392012011	1.2296024326454528E-4
30	8.60721702851817E-4	0.0	0.0	7.79998303148643	1.2296024326454528E-4
31	8.60721702851817E-4	0.0	0.0	8.004465916035368	1.2296024326454528E-4
32	8.60721702851817E-4	0.0	0.0	8.215096812747534	1.2296024326454528E-4
33	9.836819461163623E-4	0.0	0.0	8.420932259972384	1.2296024326454528E-4
34	9.836819461163623E-4	0.0	0.0	8.629841713278845	1.2296024326454528E-4
35	9.836819461163623E-4	0.0	0.0	8.858055924777842	1.2296024326454528E-4
36	9.836819461163623E-4	0.0	0.0	9.073974111950383	1.2296024326454528E-4
37	9.836819461163623E-4	0.0	0.0	9.28571165085193	1.2296024326454528E-4
38	9.836819461163623E-4	0.0	0.0	9.501015036808148	1.2296024326454528E-4
39	9.836819461163623E-4	0.0	0.0	9.728983327820616	1.2296024326454528E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	50	2.1827873E-11	45.0	1
TATTGCG	35	1.2113378E-7	45.0	1
TCGTATA	20	7.0320687E-4	45.0	29
ATAGTCG	25	3.889796E-5	45.0	14
TATACGG	35	1.2113378E-7	45.0	2
TGTACCG	25	3.889796E-5	45.0	1
TATCGCG	20	7.0320687E-4	45.0	32
ATACCGA	25	3.889796E-5	45.0	14
TACGTGA	25	3.889796E-5	45.0	24
CGTAAGG	90	0.0	42.5	2
CATACGA	255	0.0	40.588234	18
ACATACG	260	0.0	39.807693	17
ACGACCA	430	0.0	39.76744	28
GCGAGAC	435	0.0	39.310345	21
TAACGCC	75	0.0	39.000004	12
CATCGTT	75	0.0	39.000004	36
ACACGTG	440	0.0	38.863636	42
CGGCACG	35	6.2460367E-6	38.571426	22
CGAATAT	70	0.0	38.571426	14
GGACGAT	35	6.2460367E-6	38.571426	8
>>END_MODULE