FastQCFastQC Report
Sat 18 Jun 2016
SRR3553701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661618
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28640.4328781865064131No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17490.2643519372205714No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT16610.25105121081953635No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC15410.23291385663630676No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC14610.22082228718082034No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.17049112932235821No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC11220.16958426161319673No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG10720.16202703070351776No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC9180.13875075950170643No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT7960.12031111608208968Illumina Single End Adapter 2 (95% over 21bp)
ATATATATATATATATATATATATATATATATATATATATATATATATATA6890.10413864193537661No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT6650.1005111710987307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA207.0311123E-445.00000428
GTACCGG406.8066583E-945.0000042
CTCGTCA351.210883E-745.00000436
TTTACGT207.0311123E-445.00000429
GGCACGC207.0311123E-445.00000419
CTATACG207.0311123E-445.0000041
GACGTAG302.164099E-645.0000041
CACGCGT207.0311123E-445.00000439
GATTTCG207.0311123E-445.00000414
TACGGCA207.0311123E-445.00000432
CGTTCGG207.0311123E-445.0000042
TAGTTCG207.0311123E-445.00000419
CGTTAGA207.0311123E-445.00000423
GTAGCCG207.0311123E-445.00000443
CCGTACT302.164099E-645.00000444
TACGATG302.164099E-645.0000041
TGATTAC207.0311123E-445.00000411
GTCACGA207.0311123E-445.00000442
CGAATAT351.210883E-745.00000414
TTAAACG302.164099E-645.0000041