##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661618
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05636787390911	33.0	31.0	34.0	30.0	34.0
2	32.19560078474286	34.0	31.0	34.0	30.0	34.0
3	32.2459757745406	34.0	31.0	34.0	30.0	34.0
4	35.78701758416488	37.0	35.0	37.0	35.0	37.0
5	35.702990245126344	37.0	35.0	37.0	33.0	37.0
6	35.68958825183112	37.0	35.0	37.0	33.0	37.0
7	35.75972993479621	37.0	35.0	37.0	35.0	37.0
8	35.66455416871971	37.0	35.0	37.0	35.0	37.0
9	37.47738876511824	39.0	37.0	39.0	35.0	39.0
10	37.00631935648667	39.0	37.0	39.0	33.0	39.0
11	36.9114096049382	39.0	37.0	39.0	33.0	39.0
12	36.80622806513728	39.0	35.0	39.0	33.0	39.0
13	36.69556299858831	39.0	35.0	39.0	33.0	39.0
14	37.83010135758096	40.0	37.0	41.0	33.0	41.0
15	37.970300082524965	40.0	37.0	41.0	33.0	41.0
16	38.057202192201544	40.0	37.0	41.0	33.0	41.0
17	37.999280551617396	40.0	37.0	41.0	33.0	41.0
18	37.95474427842048	40.0	36.0	41.0	33.0	41.0
19	37.87388493057928	40.0	36.0	41.0	33.0	41.0
20	37.78586283928188	40.0	36.0	41.0	33.0	41.0
21	37.690102143532975	40.0	35.0	41.0	33.0	41.0
22	37.64136858428882	39.0	35.0	41.0	33.0	41.0
23	37.57395052734357	39.0	35.0	41.0	33.0	41.0
24	37.53455014827287	39.0	35.0	41.0	33.0	41.0
25	37.45898237351463	39.0	35.0	41.0	33.0	41.0
26	37.379959432784474	39.0	35.0	41.0	33.0	41.0
27	37.340521267559225	39.0	35.0	41.0	33.0	41.0
28	37.25249917626183	39.0	35.0	41.0	32.0	41.0
29	37.15946966376368	39.0	35.0	41.0	32.0	41.0
30	37.03601473962317	39.0	35.0	41.0	32.0	41.0
31	36.91521542642431	39.0	35.0	41.0	31.0	41.0
32	36.75904676112198	39.0	35.0	41.0	31.0	41.0
33	36.64194595673032	39.0	35.0	41.0	31.0	41.0
34	36.570244461305464	39.0	35.0	41.0	31.0	41.0
35	36.38660223875409	39.0	35.0	41.0	30.0	41.0
36	36.2817864689292	39.0	35.0	41.0	30.0	41.0
37	36.178353672360785	38.0	35.0	40.0	30.0	41.0
38	36.09004894062737	38.0	35.0	40.0	30.0	41.0
39	36.04370346635067	38.0	35.0	40.0	30.0	41.0
40	35.908572620454706	38.0	35.0	40.0	29.0	41.0
41	35.78665937141976	38.0	35.0	40.0	29.0	41.0
42	35.71556850025241	38.0	35.0	40.0	29.0	41.0
43	35.630504309133066	38.0	35.0	40.0	29.0	41.0
44	35.562498299623044	38.0	35.0	40.0	28.0	41.0
45	35.48390007527002	38.0	34.0	40.0	28.0	41.0
46	35.35830040899734	38.0	34.0	40.0	27.0	41.0
47	35.221461628915776	38.0	34.0	40.0	27.0	41.0
48	35.09973882209976	37.0	34.0	40.0	26.0	41.0
49	35.036753836806135	37.0	34.0	40.0	26.0	41.0
50	34.96117548192461	37.0	34.0	40.0	26.0	41.0
51	33.601153535726056	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	4.0
13	9.0
14	17.0
15	37.0
16	55.0
17	140.0
18	292.0
19	543.0
20	911.0
21	1440.0
22	2143.0
23	2976.0
24	3937.0
25	4998.0
26	6117.0
27	7221.0
28	8317.0
29	10148.0
30	12538.0
31	15695.0
32	20411.0
33	28047.0
34	47567.0
35	60746.0
36	55902.0
37	79016.0
38	125782.0
39	166545.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37729626461191	22.07316004099042	22.281135035624786	12.268408658772888
2	29.073423032626078	26.01954602202479	27.405391026241727	17.5016399191074
3	30.692333038097512	27.456024473336576	25.699724009927177	16.15191847863873
4	26.46088830715005	26.993521941664223	27.802901372090844	18.742688379094886
5	22.568914388665362	34.564658156217035	23.97712879637495	18.88929865874266
6	22.838254098286324	36.804621397845885	26.167818892472695	14.189305611395095
7	77.05352635508707	10.453161794267992	8.011269342732513	4.48204250791242
8	77.92502622359126	7.250860768600613	9.35161981687318	5.472493190934951
9	72.91035008116467	11.350356247865083	9.249143765737934	6.490149905232324
10	38.988509986124924	25.861448751394313	18.944466444383316	16.20557481809745
11	30.677672010132735	29.669537406781554	21.716307597435378	17.93648298565033
12	26.566538395267358	24.433736687937753	29.76521195009809	19.234512966696794
13	23.538809403613563	29.910461928182126	28.03082745632676	18.51990121187755
14	19.790574017030977	29.588372746811604	29.546656832190177	21.074396403967242
15	19.39774915434586	29.069039838698462	31.887584678772342	19.645626328183333
16	20.31096493747147	25.132478257846675	33.179568875091064	21.37698792959079
17	20.383816643440777	28.065288429274897	27.480661046102135	24.070233881182194
18	22.315747153191115	25.576541146099412	30.160303982056114	21.947407718653363
19	23.537146812813436	29.372840521267555	25.696247683708727	21.39376498221028
20	23.742401204320316	26.81638044914135	27.457233630282126	21.98398471625621
21	23.509185058447624	30.36147746887177	26.166912024763533	19.962425447917077
22	21.185034264484944	25.960599620929298	27.935908636101193	24.918457478484562
23	20.578642056292303	31.301445849417643	25.505805464784814	22.614106629505244
24	21.100090989060153	26.535856037774064	30.60920349809104	21.754849475074742
25	20.882896172715977	30.055863050884348	25.817012233645396	23.24422854275428
26	19.6581713314934	28.287622162637653	28.7880619934766	23.266144512392348
27	21.753489173511	30.73359551886436	25.357683738955107	22.155231568669535
28	19.077020274539084	28.554543558367516	29.79876605533707	22.56967011175633
29	21.359757443116724	29.217312709146366	26.331206224739955	23.091723622996955
30	21.79777454664172	27.91716670344519	28.194970511684993	22.090088238228102
31	24.413785598336197	29.39279161086911	23.189514190968204	23.003908599826488
32	22.285669374170595	28.591120555970363	26.637576365818344	22.4856337040407
33	22.941485872512537	29.99404490204317	23.598511527800028	23.46595769764426
34	22.02025942462267	26.87139709016381	26.962688439552736	24.14565504566079
35	21.726585431472543	28.326919763367986	25.77242457127829	24.17407023388118
36	23.8016498946522	28.622709781172823	25.837719046337916	21.73792127783706
37	22.04368684044267	30.6196324767464	25.588935004791285	21.74774567801964
38	22.793213002064636	28.602607546953074	27.07861636170721	21.525563089275078
39	22.859565489451615	28.102621149968716	26.287374285463816	22.750439075115853
40	22.954182020440797	25.713024736328215	28.28792445187404	23.044868791356947
41	20.690791362991938	27.673823868153523	25.962262211729424	25.67312255712511
42	21.893146800721865	26.161924252363143	28.485168178616664	23.459760768298324
43	21.629701731210456	26.317905498338924	28.410502737229038	23.64189003322159
44	21.283127121692576	25.996420895441176	27.96492840279436	24.755523580071884
45	21.604762869208514	27.75544196197806	26.438670048275593	24.201125120537835
46	23.19329280642304	26.465120356459465	27.493357194030395	22.848229643087098
47	21.415680951848348	27.69876273015547	28.23502383550629	22.650532482489897
48	21.88014836355722	25.717559074874018	28.92031353439598	23.48197902717278
49	22.426233869090623	25.76199559262293	28.20932925041338	23.602441287873063
50	19.836673125580017	25.090460053988856	29.901695540326894	25.171171280104232
51	20.84329628274926	26.7891744178665	26.127765568651395	26.23976373073284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5596.0
1	4817.0
2	4038.0
3	3758.5
4	3479.0
5	3025.0
6	2571.0
7	4379.0
8	6187.0
9	6659.5
10	7132.0
11	5316.5
12	3501.0
13	3395.0
14	3289.0
15	3232.5
16	3176.0
17	3049.0
18	2922.0
19	2939.5
20	2957.0
21	3005.5
22	3054.0
23	2793.5
24	2533.0
25	3736.5
26	5399.5
27	5859.0
28	6878.0
29	7897.0
30	8774.5
31	9652.0
32	11123.0
33	12594.0
34	14455.0
35	16316.0
36	17533.0
37	18750.0
38	19116.0
39	19482.0
40	23159.5
41	26837.0
42	30895.5
43	34954.0
44	39520.0
45	44086.0
46	49129.0
47	54172.0
48	55793.0
49	57414.0
50	56201.5
51	54989.0
52	49803.5
53	44618.0
54	40654.5
55	36691.0
56	32964.5
57	29238.0
58	27674.5
59	26111.0
60	25134.0
61	24157.0
62	22326.5
63	20496.0
64	18388.0
65	16280.0
66	14579.5
67	12879.0
68	10752.5
69	8626.0
70	8043.0
71	7460.0
72	6491.0
73	5522.0
74	4958.0
75	3798.5
76	3203.0
77	2383.0
78	1563.0
79	1266.0
80	969.0
81	698.5
82	428.0
83	358.5
84	289.0
85	235.5
86	182.0
87	149.0
88	116.0
89	62.5
90	9.0
91	6.5
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	661618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.984895815609597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.96570928326432	19.479761454689005
2	10.302937227492661	5.354415009023248
3	3.828364786275652	2.9843898034656395
4	1.8532986605003867	1.9263109043324553
5	1.1394299537359813	1.4803984318507168
6	0.7703649523206091	1.2010711815628852
7	0.5469909631232321	0.9949452232185997
8	0.43993436361123167	0.9145318883315493
9	0.35974350586242554	0.8413107768159541
>10	4.0588870663527485	25.44525562760368
>50	1.426936097802806	25.937044318460117
>100	0.2980346630207748	10.703095069697458
>500	0.00468423831859764	0.8206933557835283
>1k	0.00468423831859764	1.9167769551651632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2864	0.4328781865064131	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1749	0.2643519372205714	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	1661	0.25105121081953635	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1541	0.23291385663630676	No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	1461	0.22082228718082034	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1128	0.17049112932235821	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC	1122	0.16958426161319673	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG	1072	0.16202703070351776	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC	918	0.13875075950170643	No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	796	0.12031111608208968	Illumina Single End Adapter 2 (95% over 21bp)
ATATATATATATATATATATATATATATATATATATATATATATATATATA	689	0.10413864193537661	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	665	0.1005111710987307	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5343385458073993E-4	0.0	0.0	0.1260546115734457	0.0
2	4.5343385458073993E-4	0.0	0.0	0.5994395557557382	0.0
3	4.5343385458073993E-4	0.0	0.0	0.7997061748622317	0.0
4	4.5343385458073993E-4	0.0	0.0	1.138874698088625	0.0
5	4.5343385458073993E-4	0.0	0.0	2.2150243796269145	0.0
6	4.5343385458073993E-4	0.0	0.0	2.883234736660732	0.0
7	4.5343385458073993E-4	0.0	0.0	3.540562681184611	0.0
8	4.5343385458073993E-4	0.0	0.0	4.610515433376964	0.0
9	4.5343385458073993E-4	0.0	0.0	5.077854592831513	0.0
10	4.5343385458073993E-4	0.0	0.0	6.173350785498581	0.0
11	4.5343385458073993E-4	0.0	0.0	7.377217669410445	0.0
12	4.5343385458073993E-4	0.0	0.0	8.379911066506654	0.0
13	4.5343385458073993E-4	0.0	0.0	8.761097793590864	0.0
14	4.5343385458073993E-4	0.0	0.0	8.946854529350773	0.0
15	4.5343385458073993E-4	0.0	0.0	9.240528522500899	0.0
16	4.5343385458073993E-4	0.0	0.0	9.852210792330318	0.0
17	4.5343385458073993E-4	0.0	0.0	10.577553815041307	0.0
18	4.5343385458073993E-4	0.0	0.0	11.420487350706903	0.0
19	4.5343385458073993E-4	0.0	0.0	11.847319752485573	0.0
20	4.5343385458073993E-4	0.0	0.0	12.27566360044618	0.0
21	6.0457847277432E-4	0.0	0.0	12.760233246374796	0.0
22	6.0457847277432E-4	0.0	0.0	13.277752419069614	0.0
23	6.0457847277432E-4	0.0	0.0	13.809781475111016	0.0
24	6.0457847277432E-4	0.0	0.0	14.208198688669292	0.0
25	6.0457847277432E-4	0.0	0.0	14.598454092845117	0.0
26	6.0457847277432E-4	0.0	0.0	14.9237173111977	0.0
27	6.0457847277432E-4	0.0	0.0	15.285406382534937	0.0
28	6.0457847277432E-4	0.0	0.0	15.653745817072691	0.0
29	6.0457847277432E-4	0.0	0.0	16.0302470609929	0.0
30	6.0457847277432E-4	0.0	0.0	16.45375428117131	0.0
31	6.0457847277432E-4	0.0	0.0	16.831162392800678	0.0
32	6.0457847277432E-4	0.0	0.0	17.21537201224876	0.0
33	6.0457847277432E-4	0.0	0.0	17.616207539698134	0.0
34	6.0457847277432E-4	0.0	0.0	17.99678968830957	0.0
35	6.0457847277432E-4	0.0	0.0	18.416820582269526	0.0
36	6.0457847277432E-4	0.0	0.0	18.79528670622625	0.0
37	6.0457847277432E-4	0.0	0.0	19.185088676547494	0.0
38	6.0457847277432E-4	0.0	0.0	19.57201889912306	0.0
39	6.0457847277432E-4	0.0	0.0	19.934161404314878	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.0311123E-4	45.000004	28
GTACCGG	40	6.8066583E-9	45.000004	2
CTCGTCA	35	1.210883E-7	45.000004	36
TTTACGT	20	7.0311123E-4	45.000004	29
GGCACGC	20	7.0311123E-4	45.000004	19
CTATACG	20	7.0311123E-4	45.000004	1
GACGTAG	30	2.164099E-6	45.000004	1
CACGCGT	20	7.0311123E-4	45.000004	39
GATTTCG	20	7.0311123E-4	45.000004	14
TACGGCA	20	7.0311123E-4	45.000004	32
CGTTCGG	20	7.0311123E-4	45.000004	2
TAGTTCG	20	7.0311123E-4	45.000004	19
CGTTAGA	20	7.0311123E-4	45.000004	23
GTAGCCG	20	7.0311123E-4	45.000004	43
CCGTACT	30	2.164099E-6	45.000004	44
TACGATG	30	2.164099E-6	45.000004	1
TGATTAC	20	7.0311123E-4	45.000004	11
GTCACGA	20	7.0311123E-4	45.000004	42
CGAATAT	35	1.210883E-7	45.000004	14
TTAAACG	30	2.164099E-6	45.000004	1
>>END_MODULE