FastQCFastQC Report
Sat 18 Jun 2016
SRR3553697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684989
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21380.31212180049606636No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.19138993472887886No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11960.17460134396318774No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC9730.1420460766523258No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9410.13737446878709No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC9240.1348926771086835Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7160.10452722598465085No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT7130.104089262747285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTT207.0312875E-445.00000427
CGGCATA207.0312875E-445.00000443
ATTCGAT207.0312875E-445.00000419
ATATGCG302.1642118E-645.0000041
TAACGGT207.0312875E-445.00000440
CTACGAA1150.045.00000411
ACTCGCA207.0312875E-445.00000424
GTTCACG207.0312875E-445.0000041
CGCAAAC302.1642118E-645.00000414
CGCTTAG207.0312875E-445.00000410
AATCATC253.8891485E-545.040
TTTACGT453.8380676E-1045.026
TAGACCG253.8891485E-545.016
TAGCGCA253.8891485E-545.024
GCGATAT551.8189894E-1245.09
TTACGTT453.8380676E-1045.027
TATCGAG253.8891485E-545.01
TACGAAT1200.043.12500412
GCTACGA1200.043.12500410
CGAATAT1150.043.0434814