FastQCFastQC Report
Sat 18 Jun 2016
SRR3553696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567557
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG52940.932769748236741No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC32710.5763297783306347No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC31250.5506054898450728No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20570.36243055763562076No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.22605658991079308No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC12250.21583735201926857No Hit
ATATATATACATATATATATGTATATATACATATATATATGTATATATACA11980.21108012058700712No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11570.20385617656023977No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC10440.18394628204744193TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT9530.16791265018315343No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCAGCTTT9530.16791265018315343No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT9190.16192206245363902No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC7910.13936926158958485No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTC7830.13795971153558145No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTA7170.1263309235900535No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6810.11998794834703827No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6770.11928317332003657No Hit
GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT6480.11417355437427429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG302.1635515E-645.0000041
GTCGCTC302.1635515E-645.00000422
TAACGCG302.1635515E-645.0000041
GTTAACG302.1635515E-645.0000041
CGTAAGC551.8189894E-1245.00000443
CTTAACG207.030266E-445.020
AACGTAA207.030266E-445.039
AGTCCGC207.030266E-445.017
ACGTTTA207.030266E-445.022
CATGCGA207.030266E-445.013
ACGCACT207.030266E-445.011
CGCATGA207.030266E-445.016
GGCCGTA207.030266E-445.042
ATTACGA207.030266E-445.014
TAGCGAA207.030266E-445.014
CGTCTCA351.2104829E-745.018
TAACGAA207.030266E-445.034
ATCGTCC207.030266E-445.012
TTAACGG351.2104829E-745.02
TCGTAAG207.030266E-445.042