##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553696_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567557
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.089018371723014	33.0	31.0	34.0	30.0	34.0
2	32.23747394534822	34.0	31.0	34.0	30.0	34.0
3	32.26916944025005	34.0	31.0	34.0	30.0	34.0
4	35.82768779171079	37.0	35.0	37.0	35.0	37.0
5	35.73339594084823	37.0	35.0	37.0	35.0	37.0
6	35.704130157851985	37.0	35.0	37.0	33.0	37.0
7	35.79091967855211	37.0	35.0	37.0	35.0	37.0
8	35.614345343287106	37.0	35.0	37.0	35.0	37.0
9	37.479366830115744	39.0	37.0	39.0	35.0	39.0
10	37.0515366738495	39.0	37.0	39.0	33.0	39.0
11	36.937636219798186	39.0	37.0	39.0	33.0	39.0
12	36.79475541663657	39.0	35.0	39.0	33.0	39.0
13	36.657225617867454	39.0	35.0	39.0	32.0	39.0
14	37.80873286735958	40.0	36.0	41.0	33.0	41.0
15	37.89660245578858	40.0	36.0	41.0	33.0	41.0
16	38.01970550975497	40.0	37.0	41.0	33.0	41.0
17	37.875674161361765	40.0	36.0	41.0	33.0	41.0
18	37.87664675089903	40.0	36.0	41.0	33.0	41.0
19	37.77170046356577	40.0	36.0	41.0	33.0	41.0
20	37.53010710818473	40.0	35.0	41.0	32.0	41.0
21	37.54799253643247	39.0	35.0	41.0	33.0	41.0
22	37.4855882316666	39.0	35.0	41.0	33.0	41.0
23	37.30842717119162	39.0	35.0	41.0	32.0	41.0
24	37.29846165230981	39.0	35.0	41.0	32.0	41.0
25	37.28962905928391	39.0	35.0	41.0	32.0	41.0
26	37.133285291168995	39.0	35.0	41.0	32.0	41.0
27	37.03382920129608	39.0	35.0	41.0	31.0	41.0
28	36.93817361075627	39.0	35.0	41.0	31.0	41.0
29	36.85354070163878	39.0	35.0	41.0	31.0	41.0
30	36.76062140014131	39.0	35.0	41.0	31.0	41.0
31	36.67354292168012	39.0	35.0	41.0	31.0	41.0
32	36.434056843629804	39.0	35.0	41.0	30.0	41.0
33	36.29635613691664	39.0	35.0	41.0	30.0	41.0
34	36.112124420983264	39.0	35.0	41.0	30.0	41.0
35	35.920321659322326	39.0	35.0	41.0	29.0	41.0
36	35.78862387390165	38.0	35.0	41.0	28.0	41.0
37	35.67446441502792	38.0	35.0	40.0	27.0	41.0
38	35.664335035952334	38.0	35.0	40.0	27.0	41.0
39	35.530355541381745	38.0	35.0	40.0	27.0	41.0
40	35.40285997705957	38.0	35.0	40.0	26.0	41.0
41	35.36924397020916	38.0	34.0	40.0	26.0	41.0
42	35.16161196144176	38.0	34.0	40.0	26.0	41.0
43	35.05741273563712	38.0	34.0	40.0	25.0	41.0
44	35.07050921757639	38.0	34.0	40.0	25.0	41.0
45	34.937269736784145	37.0	34.0	40.0	25.0	41.0
46	34.823314662668245	37.0	34.0	40.0	24.0	41.0
47	34.64895684486316	37.0	34.0	40.0	24.0	41.0
48	34.558553590212085	37.0	34.0	40.0	23.0	41.0
49	34.49869704716883	37.0	34.0	40.0	24.0	41.0
50	34.39623509180576	37.0	34.0	40.0	24.0	41.0
51	33.06907852427157	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	3.0
13	12.0
14	13.0
15	40.0
16	103.0
17	202.0
18	323.0
19	628.0
20	1088.0
21	1929.0
22	3265.0
23	4292.0
24	5111.0
25	5629.0
26	6386.0
27	7011.0
28	7825.0
29	9142.0
30	10850.0
31	13685.0
32	17639.0
33	23969.0
34	40528.0
35	50888.0
36	47846.0
37	65372.0
38	101837.0
39	141878.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82746754951485	19.025225660153957	22.878935507799216	12.268371282531975
2	30.59886495981901	28.28403138363195	24.71293632181437	16.404167334734662
3	30.60274122246752	29.533597506505956	24.70853147789561	15.15512979313091
4	25.985407633065932	25.05034736599143	30.141818354808414	18.822426646134222
5	26.906900980870645	30.957595448562873	23.552876627369585	18.582626943196896
6	23.07592012784619	38.63470981769232	24.194398095697878	14.094971958763614
7	76.37946497003824	7.843441275501845	11.922679131787644	3.85441462267226
8	77.44790391097281	9.3863700033653	8.90712298500415	4.258603100657731
9	72.53791249160876	9.04473030902623	8.976190937650316	9.441166261714683
10	41.555473723344086	24.639463525249447	16.380557371330102	17.424505380076365
11	33.578301386468674	26.708506810769666	21.32473037950373	18.38846142325793
12	26.913067762356906	21.656855610978283	29.707148356905122	21.722928269759688
13	25.33648602695412	26.23049314870577	29.79876911041534	18.63425171392477
14	20.62013154625879	30.108517734782588	28.867585105989352	20.40376561296927
15	19.56807862470201	25.784546750370446	32.847273489711164	21.800101135216373
16	21.52241977457771	22.66873635599596	31.653384593970298	24.155459275456035
17	21.812434698188905	26.32123293343224	27.633347839952638	24.23298452842622
18	23.019009544415802	24.12444917426796	28.852432442908814	24.00410883840742
19	23.634278142988283	28.108190014395028	24.813366763162115	23.444165079454574
20	25.852733734232864	25.289089906388256	27.625947702169125	21.23222865720976
21	24.85723900859297	29.095227439710904	25.54298510986562	20.50454844183051
22	22.490780661678034	23.902621234519174	28.331603697954566	25.274994405848222
23	23.86614912687184	28.040355418046115	25.352519658818412	22.740975796263633
24	22.206756325796352	27.672462853951235	27.566746599900977	22.554034220351436
25	22.890564295744745	29.138394910114755	25.61222925626853	22.35881153787197
26	20.4587380650754	26.04425634782057	29.006249592551935	24.49075599455209
27	24.00252309459667	26.60490488180042	26.555042048640047	22.837529974962866
28	19.80400206499083	27.849537579485407	30.199609907022555	22.14685044850121
29	23.06341037111691	26.970506927057546	27.446758651553942	22.519324050271603
30	23.10886836035852	27.6580149658977	27.29012240180281	21.942994271940968
31	25.350052946223904	25.91475393660901	22.736394758588123	25.998798358578963
32	25.666496933347666	28.319974910009037	24.816714444540374	21.196813712102923
33	25.085233729828015	26.45901645121107	23.22638959611105	25.229360222849863
34	22.849334956665146	25.472507607165447	26.474345308048353	25.20381212812105
35	25.170863895608726	25.562718810621664	25.790889725613464	23.47552756815615
36	24.06648142829707	27.744526100462156	25.88321525415068	22.30577721709009
37	22.62979753575412	29.34806548064776	25.76833692474941	22.253800058848714
38	23.760961454091838	27.705058698950065	24.855124683511963	23.678855163446137
39	23.807476605873948	27.059660967973258	25.037132834235155	24.09572959191764
40	23.40505006545598	23.429012416374036	29.458891353643775	23.707046164526204
41	21.0814068014314	25.088757604963025	27.330999353368913	26.498836240236663
42	21.52700081225322	24.479655787876812	30.24894415891267	23.744399240957296
43	22.92650782212183	24.394554203366358	28.109951951962536	24.56898602254928
44	23.802719374441686	23.94984116132829	27.93552013277962	24.31191933145041
45	23.139878461546594	25.015108614641353	27.064065811892025	24.78094711192004
46	24.88701575348379	26.008489015200237	27.01173626613714	22.092758965178827
47	22.033381669153933	26.303084976486947	29.249925558137775	22.41360779622135
48	22.51773830646085	26.86990029195305	26.63820550182625	23.974155899759847
49	21.916741402185156	25.340890870872883	29.43052415880696	23.311843568135007
50	22.545929307540916	24.27897109893808	28.71376795634624	24.46133163717477
51	21.986161742344823	24.99132245748004	26.62252425747546	26.399991542699674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3523.0
1	3260.0
2	2997.0
3	3115.0
4	3233.0
5	2536.5
6	1840.0
7	2312.5
8	2785.0
9	3966.5
10	5148.0
11	3892.5
12	2637.0
13	2404.0
14	2171.0
15	2325.5
16	2480.0
17	2219.0
18	1958.0
19	2087.5
20	2217.0
21	2421.0
22	2625.0
23	2819.5
24	3014.0
25	3343.5
26	4041.0
27	4409.0
28	5480.0
29	6551.0
30	7944.0
31	9337.0
32	9879.0
33	10421.0
34	11425.0
35	12429.0
36	12207.0
37	11985.0
38	12961.5
39	13938.0
40	16713.5
41	19489.0
42	21922.5
43	24356.0
44	28225.5
45	32095.0
46	41919.0
47	51743.0
48	49110.5
49	46478.0
50	44400.5
51	42323.0
52	40319.5
53	38316.0
54	35927.0
55	33538.0
56	31770.0
57	30002.0
58	28970.5
59	27939.0
60	26295.0
61	24651.0
62	22682.0
63	20713.0
64	18400.0
65	16087.0
66	14403.5
67	12720.0
68	11588.5
69	10457.0
70	9613.5
71	8770.0
72	7354.0
73	5938.0
74	5416.0
75	4013.5
76	3133.0
77	2692.5
78	2252.0
79	1796.0
80	1340.0
81	940.0
82	540.0
83	390.5
84	241.0
85	179.5
86	118.0
87	74.5
88	31.0
89	20.5
90	10.0
91	6.5
92	3.0
93	3.5
94	4.0
95	4.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	567557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.981636435336895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.65635381834895	19.150011615485425
2	8.63199435836724	4.312826895452192
3	3.0312626067402793	2.2717770114485174
4	1.5449597452611752	1.5438249065338154
5	1.0201788156342746	1.2742868135604017
6	0.7191131380712126	1.0778773782701554
7	0.5348095830538815	0.9352292996190328
8	0.42209799103944556	0.8435758841786817
9	0.4071687400948971	0.915456728959641
>10	5.425484879953944	32.935750554320336
>50	1.4312877496503984	23.88212285267017
>100	0.16256601600967488	6.129357583396717
>500	0.006361278887335104	1.2575486045271633
>1k	0.005654470122075648	2.535579606993831
>5k	7.06808765259456E-4	0.9347742645839376
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG	5294	0.932769748236741	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC	3271	0.5763297783306347	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC	3125	0.5506054898450728	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2057	0.36243055763562076	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1283	0.22605658991079308	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1225	0.21583735201926857	No Hit
ATATATATACATATATATATGTATATATACATATATATATGTATATATACA	1198	0.21108012058700712	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1157	0.20385617656023977	No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	1044	0.18394628204744193	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT	953	0.16791265018315343	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCAGCTTT	953	0.16791265018315343	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	919	0.16192206245363902	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC	791	0.13936926158958485	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTC	783	0.13795971153558145	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTA	717	0.1263309235900535	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	681	0.11998794834703827	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	677	0.11928317332003657	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT	648	0.11417355437427429	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05708677718713715	0.0
2	0.0	0.0	0.0	0.4195173348227579	0.0
3	0.0	0.0	0.0	0.5553627212773342	0.0
4	0.0	0.0	0.0	0.8607065017258179	0.0
5	0.0	0.0	0.0	2.0339807279268864	0.0
6	0.0	0.0	0.0	2.5126991650177866	0.0
7	0.0	0.0	0.0	3.085505068213413	0.0
8	0.0	0.0	0.0	3.8103661834846543	0.0
9	0.0	0.0	0.0	4.07941405004255	0.0
10	0.0	0.0	0.0	5.6429574474458075	0.0
11	0.0	0.0	0.0	6.513002218279397	0.0
12	0.0	0.0	0.0	7.765035053747906	0.0
13	0.0	0.0	0.0	8.061216758845367	0.0
14	0.0	0.0	0.0	8.200233632921451	0.0
15	0.0	0.0	0.0	8.50751554469419	0.0
16	0.0	0.0	0.0	8.912761185220162	0.0
17	0.0	0.0	0.0	9.363641008744496	0.0
18	0.0	0.0	0.0	9.896627122914527	0.0
19	0.0	0.0	0.0	10.414989155274272	0.0
20	0.0	0.0	0.0	10.719099579425503	0.0
21	0.0	0.0	0.0	11.043472285603032	0.0
22	0.0	0.0	0.0	11.42951280664321	0.0
23	0.0	0.0	0.0	11.765866688279768	0.0
24	0.0	0.0	0.0	12.033857392297161	0.0
25	0.0	0.0	0.0	12.27471425777499	0.0
26	0.0	0.0	0.0	12.517685448333824	0.0
27	0.0	0.0	0.0	12.777042658270448	0.0
28	0.0	0.0	0.0	13.024418692748041	0.0
29	0.0	0.0	0.0	13.270032789658131	0.0
30	0.0	0.0	0.0	13.56075248829633	0.0
31	0.0	0.0	0.0	13.82768602977322	0.0
32	0.0	0.0	0.0	14.082638395791083	0.0
33	0.0	0.0	0.0	14.33477166170094	0.0
34	0.0	0.0	0.0	14.589900221475553	0.0
35	0.0	0.0	0.0	14.867933969627721	0.0
36	0.0	0.0	0.0	15.12570543575359	0.0
37	0.0	0.0	0.0	15.388762714581972	0.0
38	0.0	0.0	0.0	15.656753418599365	0.0
39	0.0	0.0	0.0	15.948001698507815	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1635515E-6	45.000004	1
GTCGCTC	30	2.1635515E-6	45.000004	22
TAACGCG	30	2.1635515E-6	45.000004	1
GTTAACG	30	2.1635515E-6	45.000004	1
CGTAAGC	55	1.8189894E-12	45.000004	43
CTTAACG	20	7.030266E-4	45.0	20
AACGTAA	20	7.030266E-4	45.0	39
AGTCCGC	20	7.030266E-4	45.0	17
ACGTTTA	20	7.030266E-4	45.0	22
CATGCGA	20	7.030266E-4	45.0	13
ACGCACT	20	7.030266E-4	45.0	11
CGCATGA	20	7.030266E-4	45.0	16
GGCCGTA	20	7.030266E-4	45.0	42
ATTACGA	20	7.030266E-4	45.0	14
TAGCGAA	20	7.030266E-4	45.0	14
CGTCTCA	35	1.2104829E-7	45.0	18
TAACGAA	20	7.030266E-4	45.0	34
ATCGTCC	20	7.030266E-4	45.0	12
TTAACGG	35	1.2104829E-7	45.0	2
TCGTAAG	20	7.030266E-4	45.0	42
>>END_MODULE