##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553695_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1103276
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.087259217095266	33.0	31.0	34.0	30.0	34.0
2	32.2161100214271	34.0	31.0	34.0	30.0	34.0
3	32.26600052933264	34.0	31.0	34.0	30.0	34.0
4	35.81296792461723	37.0	35.0	37.0	35.0	37.0
5	35.732110550759735	37.0	35.0	37.0	35.0	37.0
6	35.69859944383817	37.0	35.0	37.0	33.0	37.0
7	35.8844749636537	37.0	35.0	37.0	35.0	37.0
8	35.80194529745957	37.0	35.0	37.0	35.0	37.0
9	37.58783477570436	39.0	37.0	39.0	35.0	39.0
10	37.04550629216987	39.0	37.0	39.0	33.0	39.0
11	36.94620022551021	39.0	37.0	39.0	33.0	39.0
12	36.87836316569925	39.0	37.0	39.0	33.0	39.0
13	36.87355475873671	39.0	37.0	39.0	33.0	39.0
14	38.01734561433404	40.0	37.0	41.0	33.0	41.0
15	38.10127656180321	40.0	37.0	41.0	33.0	41.0
16	38.16028718108615	40.0	37.0	41.0	33.0	41.0
17	38.11464583658124	40.0	37.0	41.0	33.0	41.0
18	38.084970578531575	40.0	37.0	41.0	33.0	41.0
19	38.03200105866529	40.0	37.0	41.0	33.0	41.0
20	37.965713021945554	40.0	36.0	41.0	33.0	41.0
21	37.873519409467804	40.0	36.0	41.0	33.0	41.0
22	37.81547137796888	40.0	36.0	41.0	33.0	41.0
23	37.729419474365436	40.0	36.0	41.0	33.0	41.0
24	37.708407506371934	40.0	36.0	41.0	33.0	41.0
25	37.59532338236307	40.0	36.0	41.0	33.0	41.0
26	37.5366154978446	40.0	36.0	41.0	33.0	41.0
27	37.48242688139686	39.0	36.0	41.0	33.0	41.0
28	37.39709555904415	39.0	35.0	41.0	32.0	41.0
29	37.31855492188718	39.0	35.0	41.0	32.0	41.0
30	37.198076455936686	39.0	35.0	41.0	32.0	41.0
31	37.06996979903487	39.0	35.0	41.0	31.0	41.0
32	36.95283682414917	39.0	35.0	41.0	31.0	41.0
33	36.8419271333737	39.0	35.0	41.0	31.0	41.0
34	36.76582378298812	39.0	35.0	41.0	31.0	41.0
35	36.6112967199504	39.0	35.0	41.0	30.0	41.0
36	36.513005811782364	39.0	35.0	41.0	30.0	41.0
37	36.43020060256907	39.0	35.0	41.0	30.0	41.0
38	36.316308883724474	39.0	35.0	41.0	30.0	41.0
39	36.25284969490862	39.0	35.0	41.0	30.0	41.0
40	36.136866024458065	39.0	35.0	40.0	30.0	41.0
41	35.99877183950344	38.0	35.0	40.0	29.0	41.0
42	35.886542442688864	38.0	35.0	40.0	29.0	41.0
43	35.790576428744934	38.0	35.0	40.0	29.0	41.0
44	35.685923558565584	38.0	35.0	40.0	28.0	41.0
45	35.586274875915	38.0	34.0	40.0	28.0	41.0
46	35.47162632015924	38.0	34.0	40.0	28.0	41.0
47	35.34376529535674	38.0	34.0	40.0	27.0	41.0
48	35.18133540474007	37.0	34.0	40.0	27.0	41.0
49	35.11023533549175	37.0	34.0	40.0	27.0	41.0
50	35.03685931716089	37.0	34.0	40.0	27.0	41.0
51	33.593295784554364	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	10.0
13	16.0
14	29.0
15	57.0
16	126.0
17	242.0
18	445.0
19	715.0
20	1235.0
21	1901.0
22	2842.0
23	4070.0
24	5361.0
25	7254.0
26	9325.0
27	11731.0
28	14037.0
29	16970.0
30	20783.0
31	26337.0
32	33248.0
33	45542.0
34	74755.0
35	89702.0
36	95890.0
37	137569.0
38	217332.0
39	285651.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36650484556902	17.695390817891443	24.549795336796958	13.388308999742584
2	29.85028225031633	24.773311483255323	26.7113578107382	18.665048455690144
3	32.52875980262419	24.152252020346676	26.923997259072074	16.394990917957067
4	28.328178986944337	26.30629144475181	26.06337851997143	19.302151048332423
5	24.53193942404258	30.310547859284533	24.930661049456347	20.226851667216543
6	24.992748867917	36.453797599150164	24.468673296618434	14.084780236314396
7	82.00713148840363	6.2857344852965165	7.414010637410766	4.29312338888909
8	83.02845344229368	5.046878568916572	7.4232558308165855	4.501412157973164
9	77.8157958661296	7.298445719838009	9.461005224440665	5.424753189591725
10	39.79249072761485	25.134417860988545	17.869508627034396	17.203582784362208
11	30.143409264771464	26.47197981284828	22.657159223983843	20.72745169839641
12	25.494074012305173	23.170811292913108	30.142140316656942	21.192974378124784
13	24.28929841671531	23.95647145410577	30.684343718163	21.06988641101592
14	21.150102059684066	26.70537562676973	29.465609693313365	22.678912620232833
15	20.55686881614392	25.815299163581912	32.172094743291794	21.45573727698237
16	22.897987448290362	23.59962511647131	30.700477487047667	22.80190994819066
17	22.645104216895863	23.63497438537592	28.19802116605455	25.521900231673673
18	23.414086774297637	24.267454381315282	29.256777089323073	23.061681755064008
19	23.747548210964435	27.61847443432106	26.161087524789806	22.472889829924696
20	25.17864976669483	25.52942328120978	26.28091248246132	23.011014469634073
21	25.214633509656696	26.215017819657092	26.587091534665852	21.983257136020363
22	23.156218389596077	24.154517999122614	26.26713533150363	26.42212827977768
23	22.19353996642726	27.153767506952022	26.026941581254377	24.625750945366345
24	22.327142075056468	25.71876846772702	28.286575616618144	23.667513840598364
25	23.91668086680033	26.06618833365359	26.33846834337011	23.67866245617597
26	22.293152393417422	25.583262936926026	27.186669518778615	24.93691515087793
27	22.86082539636501	25.68260344646308	27.856402205794378	23.600168951377533
28	21.418937781661164	25.765900826266503	29.639455585003212	23.17570580706913
29	23.042194337590956	25.883731722615195	26.80009353960387	24.27398040018998
30	24.2597500534771	24.862953603631365	27.547775896511844	23.329520446379693
31	25.07804030904325	26.584462999285762	24.600825178831045	23.73667151283994
32	24.55858733444759	26.88991693828199	25.74704788285071	22.80444784441971
33	23.73848429586069	25.94264717078954	24.855974389001485	25.462894144348287
34	23.33405240393156	25.419206073548235	25.961046918450144	25.28569460407006
35	23.34955169875897	24.377037114919567	26.717249355555634	25.556161830765827
36	24.457887237644975	26.4078979330648	26.10144696340716	23.03276786588306
37	22.82339142698654	27.619018269227286	26.36837926321247	23.18921104057371
38	23.241600469873358	27.739387061805026	26.08912003886607	22.92989242945555
39	23.247854571294944	25.505313267033813	25.474586594832115	25.772245566839125
40	22.895902747816503	24.414743001751148	28.617227239602784	24.072127010829565
41	21.436974972717614	25.477849604269466	28.099224491423726	24.985950931589194
42	22.095377765853694	26.004916267552268	27.79077946044326	24.108926506150773
43	22.92381960633604	24.59312085099286	27.970154340346387	24.512905202324713
44	22.61102389610578	24.67723398315562	27.223559653250863	25.488182467487736
45	23.57859683343062	24.372505157367694	26.821393740097673	25.227504269104013
46	23.502006750803968	24.482269169274055	27.50055289882133	24.51517118110065
47	22.30711082267719	25.426910401386415	28.581877970698176	23.684100805238216
48	22.29605284625062	25.527519859037994	27.753345491064792	24.423081803646593
49	22.22055043343642	25.592417491180814	28.218143057584864	23.968889017797903
50	21.909204949622758	23.782534923264894	29.128341412302994	25.17991871480935
51	22.481409910122217	24.49160500183091	26.6951334027025	26.331851685344375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3434.0
1	3414.0
2	3394.0
3	4065.5
4	4737.0
5	3632.0
6	2527.0
7	2956.0
8	3385.0
9	3636.0
10	3887.0
11	3541.5
12	3196.0
13	3178.5
14	3161.0
15	3023.5
16	2886.0
17	3064.5
18	3243.0
19	3353.5
20	3464.0
21	3488.5
22	3513.0
23	3873.0
24	4233.0
25	5355.0
26	7820.5
27	9164.0
28	10755.5
29	12347.0
30	13637.0
31	14927.0
32	18719.0
33	22511.0
34	23907.0
35	25303.0
36	28510.5
37	31718.0
38	33262.0
39	34806.0
40	38147.5
41	41489.0
42	46421.0
43	51353.0
44	57403.5
45	63454.0
46	70672.0
47	77890.0
48	85860.0
49	93830.0
50	91983.0
51	90136.0
52	85702.0
53	81268.0
54	73956.5
55	66645.0
56	63481.0
57	60317.0
58	56948.5
59	53580.0
60	50507.0
61	47434.0
62	44283.0
63	41132.0
64	37670.5
65	34209.0
66	30224.5
67	26240.0
68	23813.0
69	21386.0
70	18688.5
71	15991.0
72	14146.0
73	12301.0
74	10570.0
75	7330.5
76	5822.0
77	4520.0
78	3218.0
79	2698.5
80	2179.0
81	1647.5
82	1116.0
83	812.5
84	509.0
85	378.5
86	248.0
87	201.5
88	155.0
89	132.0
90	109.0
91	78.0
92	47.0
93	43.5
94	40.0
95	24.5
96	9.0
97	13.0
98	17.0
99	8.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1103276.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.25786534054209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68285928460801	18.610717377167408
2	10.161067767172257	5.132937627587191
3	3.622436505490765	2.7448504038104877
4	1.8728260760092146	1.8921435533638633
5	1.1247814559353486	1.4204789275776957
6	0.8127270277495364	1.2316649895310081
7	0.6186322938811426	1.0937731821912393
8	0.48692147426005844	0.983887762262303
9	0.4133782587190956	0.9396947154091219
>10	5.6452978621956165	35.26483828591784
>50	1.4239716968636198	23.801017487991057
>100	0.12751445155541322	4.949633956718567
>500	0.004695999632352143	0.8680495025570828
>1k	0.0028898459276013185	1.0663122279151382
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2056	0.18635409453300897	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1822	0.16514453319024433	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1545	0.1400374883528691	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1379	0.12499138928065143	TruSeq Adapter, Index 16 (95% over 22bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1378	0.12490075012961399	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC	1190	0.10786058973457231	No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	1168	0.10586652841174829	TruSeq Adapter, Index 13 (95% over 22bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1149	0.10414438454203663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1109423208698458	0.0
2	0.0	0.0	0.0	0.48528201465453796	0.0
3	0.0	0.0	0.0	0.6723612223958465	0.0
4	0.0	0.0	0.0	0.950714055231873	0.0
5	0.0	0.0	0.0	1.7976462825258595	0.0
6	0.0	0.0	0.0	2.475355214832916	0.0
7	0.0	0.0	0.0	3.071035715451075	0.0
8	0.0	0.0	0.0	4.013954803693727	0.0
9	0.0	0.0	0.0	4.427450610726599	0.0
10	0.0	0.0	0.0	5.352785703667985	0.0
11	0.0	0.0	0.0	6.469731961902552	0.0
12	0.0	0.0	0.0	7.311769675040516	0.0
13	0.0	0.0	0.0	7.627737755557086	0.0
14	0.0	0.0	0.0	7.755629597670937	0.0
15	0.0	0.0	0.0	7.980414692243826	0.0
16	0.0	0.0	0.0	8.489444164470179	0.0
17	0.0	0.0	0.0	9.097360950478393	0.0
18	0.0	0.0	0.0	9.810419151690057	0.0
19	0.0	0.0	0.0	10.163911840736136	0.0
20	0.0	0.0	0.0	10.518492199594661	0.0
21	0.0	0.0	0.0	10.951928619855774	0.0
22	0.0	0.0	0.0	11.412103589672938	0.0
23	0.0	0.0	0.0	11.896026017061914	0.0
24	0.0	0.0	0.0	12.302905166069053	0.0
25	0.0	0.0	0.0	12.644886682933373	0.0
26	0.0	0.0	0.0	12.939010728049917	0.0
27	0.0	0.0	0.0	13.248543428842828	0.0
28	0.0	0.0	0.0	13.569587301817496	0.0
29	9.063915103745572E-5	0.0	0.0	13.912928405947378	0.0
30	1.8127830207491144E-4	0.0	0.0	14.28690554312792	0.0
31	1.8127830207491144E-4	0.0	0.0	14.64638041614247	0.0
32	2.7191745311236717E-4	0.0	0.0	15.006036567459095	0.0
33	2.7191745311236717E-4	0.0	0.0	15.350827898005576	0.0
34	2.7191745311236717E-4	0.0	0.0	15.691449827604334	0.0
35	2.7191745311236717E-4	0.0	0.0	16.08881186575254	0.0
36	3.625566041498229E-4	0.0	0.0	16.4272584557264	0.0
37	3.625566041498229E-4	0.0	0.0	16.760266696638013	0.0
38	3.625566041498229E-4	0.0	0.0	17.100163513028473	0.0
39	3.625566041498229E-4	0.0	0.0	17.419575881284466	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	50	2.1827873E-11	45.0	1
TTCACGG	55	1.8189894E-12	45.0	2
CTAACGT	20	7.033164E-4	45.0	41
AATTGCG	20	7.033164E-4	45.0	1
TCGTACC	20	7.033164E-4	45.0	25
TCGACAT	20	7.033164E-4	45.0	15
GTACGCG	20	7.033164E-4	45.0	1
GACACGA	205	0.0	43.902435	25
TACGCAT	115	0.0	43.04348	16
TATCGCG	95	0.0	42.63158	32
CGTAAGG	180	0.0	42.5	2
CACGACC	215	0.0	41.860462	27
TATAGCG	70	0.0	41.785717	1
GCGTAAG	105	0.0	40.714287	1
GTATAAG	50	1.0822987E-9	40.5	1
ACACGAC	225	0.0	40.0	26
ACTAACG	40	3.4580626E-7	39.375	1
TCGTAAG	40	3.4580626E-7	39.375	1
ACGCATT	115	0.0	39.130436	17
TACGCGG	75	0.0	39.0	2
>>END_MODULE