FastQCFastQC Report
Sat 18 Jun 2016
SRR3553692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656398
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27350.4166679362216217No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13960.21267584605681308No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.20658198227295027No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC12700.19348017513764515No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC8230.12538124735297793No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC7140.10877546854195168No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC7130.10862312194735511No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA7000.10664261621759968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC302.1640735E-645.00000411
GTACCGG207.031073E-445.02
ACCGGAT207.031073E-445.043
TTTCGCG207.031073E-445.023
TCGGCGA207.031073E-445.013
CATTAGA253.888971E-545.022
CGTTAAG207.031073E-445.02
GCCGATA351.2108649E-745.09
TTGCGAA207.031073E-445.028
GCGATAT207.031073E-445.09
CCGACAG207.031073E-445.015
CTCGAGG502.1827873E-1145.02
GTACGAG351.2108649E-745.01
ACGCATT1200.043.12500417
AGTACGG750.042.02
TACGCAT1250.041.39999816
GCTACGA556.002665E-1140.90909210
GGCGATA1600.040.781258
TTAGGCG501.0804797E-940.51
ATAGCGG1000.040.52