##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553692_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656398
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14486637680188	33.0	31.0	34.0	30.0	34.0
2	32.29081441442539	34.0	31.0	34.0	30.0	34.0
3	32.3374477070314	34.0	31.0	34.0	30.0	34.0
4	35.855633320028396	37.0	35.0	37.0	35.0	37.0
5	35.77963065091606	37.0	35.0	37.0	35.0	37.0
6	35.738175619060385	37.0	35.0	37.0	33.0	37.0
7	35.84476643743582	37.0	35.0	37.0	35.0	37.0
8	35.72859606519216	37.0	35.0	37.0	35.0	37.0
9	37.55771193696507	39.0	37.0	39.0	35.0	39.0
10	37.07023482704091	39.0	37.0	39.0	33.0	39.0
11	36.973887793686146	39.0	37.0	39.0	33.0	39.0
12	36.85443739926082	39.0	35.0	39.0	33.0	39.0
13	36.75716105167901	39.0	35.0	39.0	33.0	39.0
14	37.922633828866026	40.0	37.0	41.0	33.0	41.0
15	38.038228330982115	40.0	37.0	41.0	33.0	41.0
16	38.126496424425426	40.0	37.0	41.0	33.0	41.0
17	38.066116898588966	40.0	37.0	41.0	33.0	41.0
18	38.00829070167794	40.0	37.0	41.0	33.0	41.0
19	37.91286384175454	40.0	36.0	41.0	33.0	41.0
20	37.82774170548966	40.0	36.0	41.0	33.0	41.0
21	37.719635038497984	40.0	36.0	41.0	33.0	41.0
22	37.70481628524158	40.0	36.0	41.0	33.0	41.0
23	37.61973680602318	39.0	35.0	41.0	33.0	41.0
24	37.58489513983894	40.0	35.0	41.0	33.0	41.0
25	37.51469839944668	39.0	35.0	41.0	33.0	41.0
26	37.4319894332402	39.0	35.0	41.0	33.0	41.0
27	37.39360113833376	39.0	35.0	41.0	33.0	41.0
28	37.29980743390443	39.0	35.0	41.0	32.0	41.0
29	37.22286478630343	39.0	35.0	41.0	32.0	41.0
30	37.07673545623235	39.0	35.0	41.0	32.0	41.0
31	36.97246335302667	39.0	35.0	41.0	31.0	41.0
32	36.834914183163264	39.0	35.0	41.0	31.0	41.0
33	36.7072431664935	39.0	35.0	41.0	31.0	41.0
34	36.581781175445386	39.0	35.0	41.0	30.0	41.0
35	36.428395577073665	39.0	35.0	41.0	30.0	41.0
36	36.28452859393234	39.0	35.0	41.0	30.0	41.0
37	36.20234522347722	39.0	35.0	41.0	30.0	41.0
38	36.11214842214632	39.0	35.0	41.0	30.0	41.0
39	36.06141091228188	38.0	35.0	41.0	29.0	41.0
40	35.89024646632074	38.0	35.0	40.0	29.0	41.0
41	35.780013040868496	38.0	35.0	40.0	28.0	41.0
42	35.666860654663786	38.0	35.0	40.0	28.0	41.0
43	35.560338392255915	38.0	35.0	40.0	28.0	41.0
44	35.461457835033016	38.0	34.0	40.0	27.0	41.0
45	35.36957303343399	38.0	34.0	40.0	27.0	41.0
46	35.2069019101216	38.0	34.0	40.0	27.0	41.0
47	35.10682390866517	37.0	34.0	40.0	26.0	41.0
48	34.94519788299172	37.0	34.0	40.0	26.0	41.0
49	34.86145905380577	37.0	34.0	40.0	26.0	41.0
50	34.77013945807269	37.0	34.0	40.0	26.0	41.0
51	33.382076118452524	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	4.0
12	7.0
13	7.0
14	25.0
15	45.0
16	74.0
17	126.0
18	283.0
19	489.0
20	869.0
21	1315.0
22	2050.0
23	2880.0
24	3915.0
25	5014.0
26	6139.0
27	7436.0
28	8818.0
29	10393.0
30	12663.0
31	15844.0
32	20009.0
33	27516.0
34	46422.0
35	57851.0
36	54619.0
37	77596.0
38	122853.0
39	171084.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80070627881255	20.297746184479536	23.964728716419	13.93681882028891
2	28.998412548484303	26.388410689855853	26.56955079083117	18.043625970828675
3	32.51563837793533	26.3640352347204	24.887035000106643	16.23329138723762
4	28.044113479931383	28.217788597771477	25.179388115137463	18.558709807159683
5	24.654554096752275	32.397112727339206	23.448426107331223	19.499907068577297
6	24.3690565784783	37.668152553785966	23.991846410257192	13.970944457478543
7	80.25862357898714	8.34463237243258	7.609560053504125	3.7871839950761577
8	81.8552158903592	6.619611881815605	7.499718158799997	4.025454069025195
9	76.84316527472662	9.277450571147384	8.906486613304732	4.97289754082127
10	40.54750319166116	26.852001377213213	16.46226831891627	16.13822711220936
11	31.284373200405852	28.4074601080442	21.565879237901395	18.742287453648547
12	27.933052812470482	24.04349190582543	28.44950776815286	19.57394751355123
13	24.56223206042675	27.243379778731807	28.026593621552777	20.167794539288664
14	21.26225247487043	29.51791443605861	28.15212721550035	21.06770587357061
15	20.309324525668877	28.02689831474197	31.05341576299745	20.610361396591703
16	23.01103903424447	24.781154116862027	30.93290960667156	21.274897242221943
17	22.29379126688381	25.467170832330382	27.054927041215848	25.18411085956996
18	23.378651367005993	25.80020048811849	28.423913540260635	22.397234604614884
19	25.12530507405568	27.989116359282022	25.740328276442035	21.145250290220265
20	25.78024308422634	26.085240966608676	26.188988997528938	21.945526951636047
21	24.252511433611925	28.352310640800244	26.07411966520312	21.321058260384707
22	22.972952385595324	26.0529434885542	25.724636577198588	25.249467548651882
23	21.900889399419256	28.488356149774987	25.81132178952404	23.79943266128172
24	21.92328434882495	27.51059570565419	28.3544434931246	22.211676452396258
25	22.81801589889061	27.40852348727449	26.970984067593136	22.802476546241763
26	20.72294552999857	28.31696623085384	26.97662089159321	23.98346734755438
27	22.279013647207943	28.51379803107261	27.461692448788693	21.745495872930753
28	21.103050283517014	28.028878820471725	28.285735178961545	22.582335717049716
29	22.569081563319816	27.82488673030692	26.879119071051406	22.726912635321863
30	22.321518347100387	28.17726440360879	26.700111822400434	22.80110542689039
31	24.38764286301908	28.892684011834284	23.843156133930936	22.876516991215695
32	24.13642332852934	28.143138766419156	25.547457487682777	22.17298041736873
33	23.636878844847182	28.84698003345531	23.88307094171524	23.633070179982266
34	22.081572460610786	28.219159717122842	25.180606887894236	24.518660934372132
35	22.604578319860817	28.493535933991264	24.245503490260482	24.656382255887436
36	23.294556046788685	29.98409501552412	25.10077727232563	21.620571665361567
37	22.07395513088096	29.839365750657375	25.505257480979527	22.581421637482137
38	22.125295933260002	29.893144098549968	24.44370640983062	23.53785355835941
39	22.9785892095954	28.322907748043107	24.828229214592366	23.87027382776913
40	22.55597975618451	26.85900932056466	27.064677223270028	23.520333699980807
41	20.652409056700353	27.856879515172196	25.401966489843055	26.088744938284396
42	21.563594038982448	28.139634794743433	25.48956578173608	24.80720538453804
43	21.669931962010853	27.975862205552122	25.949652497417723	24.4045533350193
44	21.52931605519822	27.2346960228398	26.444626583261982	24.791361338699996
45	22.551714051535807	28.3587091977733	25.203458877083722	23.88611787360717
46	22.733920578673306	27.983174842092755	26.18975073050192	23.09315384873202
47	21.81085256201268	27.855660742415424	26.824426643591238	23.509060051980658
48	21.68470958168672	27.51851772857321	26.456204924451328	24.340567765288743
49	21.89616665498676	27.72266216533262	27.10672488337868	23.27444629630194
50	21.153629352923073	26.674212901319017	27.747189967062667	24.42496777869524
51	21.496256844170762	26.78390244942855	25.571985289412822	26.147855416987863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4069.0
1	3815.0
2	3561.0
3	3600.5
4	3640.0
5	2886.0
6	2132.0
7	2406.0
8	2680.0
9	3111.5
10	3543.0
11	3159.5
12	2776.0
13	2698.0
14	2620.0
15	2546.0
16	2472.0
17	2395.0
18	2318.0
19	2479.5
20	2641.0
21	2844.0
22	3047.0
23	3234.0
24	3421.0
25	3924.5
26	5943.0
27	7458.0
28	8178.5
29	8899.0
30	10134.0
31	11369.0
32	12325.0
33	13281.0
34	14601.5
35	15922.0
36	17624.5
37	19327.0
38	19667.5
39	20008.0
40	23968.5
41	27929.0
42	30073.5
43	32218.0
44	36037.5
45	39857.0
46	43523.0
47	47189.0
48	49449.5
49	51710.0
50	50010.5
51	48311.0
52	45822.5
53	43334.0
54	40435.5
55	37537.0
56	35164.5
57	32792.0
58	30837.0
59	28882.0
60	28226.5
61	27571.0
62	26089.5
63	24608.0
64	21333.5
65	18059.0
66	16256.5
67	14454.0
68	13548.5
69	12643.0
70	10776.5
71	8910.0
72	8061.0
73	7212.0
74	6405.5
75	4521.5
76	3444.0
77	2728.0
78	2012.0
79	1657.5
80	1303.0
81	1062.0
82	821.0
83	513.5
84	206.0
85	144.0
86	82.0
87	56.0
88	30.0
89	33.5
90	37.0
91	29.0
92	21.0
93	16.5
94	12.0
95	7.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	656398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.729519896052665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.71404276791934	19.480859698077634
2	9.143048181451109	4.70492480190429
3	3.284821287734702	2.53550624033242
4	1.6236152028772637	1.6709935866385657
5	1.046108510957504	1.3457934873055564
6	0.7211505956236539	1.113291515888942
7	0.554972015611965	0.9995414462208353
8	0.441204965259171	0.9081593545498454
9	0.37481792406590136	0.867949671118569
>10	5.481407634006015	34.50749270899339
>50	1.4940718295423456	25.43414584157817
>100	0.11241225150617169	4.392184281677819
>500	0.00594773817492972	1.0051171165485493
>1k	0.002379095269971888	1.0340402491654304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2735	0.4166679362216217	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1396	0.21267584605681308	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1356	0.20658198227295027	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCC	1270	0.19348017513764515	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	823	0.12538124735297793	No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	714	0.10877546854195168	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	713	0.10862312194735511	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	700	0.10664261621759968	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.09386378386284E-4	0.0	0.0	0.09948232627156085	0.0
2	6.09386378386284E-4	0.0	0.0	0.4032614358971234	0.0
3	6.09386378386284E-4	0.0	0.0	0.5298614560068738	0.0
4	6.09386378386284E-4	0.0	0.0	0.7142008354687248	0.0
5	6.09386378386284E-4	0.0	0.0	1.285500565205866	0.0
6	6.09386378386284E-4	0.0	0.0	1.7189266268331105	0.0
7	6.09386378386284E-4	0.0	0.0	2.1506768759197925	0.0
8	6.09386378386284E-4	0.0	0.0	2.898393962199763	0.0
9	7.617329729828549E-4	0.0	0.0	3.2157319187444204	0.0
10	7.617329729828549E-4	0.0	0.0	3.9101276969155907	0.0
11	7.617329729828549E-4	0.0	0.0	4.813695349467853	0.0
12	7.617329729828549E-4	0.0	0.0	5.529267304287948	0.0
13	7.617329729828549E-4	0.0	0.0	5.787190088939942	0.0
14	7.617329729828549E-4	0.0	0.0	5.8973366768332625	0.0
15	7.617329729828549E-4	0.0	0.0	6.077410351646409	0.0
16	7.617329729828549E-4	0.0	0.0	6.517539663435903	0.0
17	7.617329729828549E-4	0.0	0.0	7.033080539550699	0.0
18	7.617329729828549E-4	0.0	0.0	7.6270799118827295	0.0
19	9.140795675794259E-4	0.0	0.0	7.936495845508365	0.0
20	9.140795675794259E-4	0.0	0.0	8.248958711025933	0.0
21	9.140795675794259E-4	0.0	0.0	8.631653356652519	0.0
22	9.140795675794259E-4	0.0	0.0	9.034457752765853	0.0
23	9.140795675794259E-4	0.0	0.0	9.429492472554761	0.0
24	9.140795675794259E-4	0.0	0.0	9.749725014396754	0.0
25	0.001218772756772568	0.0	0.0	10.034308453103147	0.0
26	0.0015234659459657097	0.0	0.0	10.309294056349959	0.0
27	0.0015234659459657097	0.0	0.0	10.575138863920975	0.0
28	0.0015234659459657097	0.0	0.0	10.854390171816489	0.0
29	0.0015234659459657097	0.0	0.0	11.143391661766184	0.0
30	0.0015234659459657097	0.0	0.0	11.456920953445927	0.0
31	0.0015234659459657097	0.0	0.0	11.748055295719976	0.0
32	0.0015234659459657097	0.0	0.0	12.045435848372481	0.0
33	0.0015234659459657097	0.0	0.0	12.338398349781688	0.0
34	0.0015234659459657097	0.0	0.0	12.646138470866761	0.0
35	0.0015234659459657097	0.0	0.0	12.970332024168263	0.0
36	0.0015234659459657097	0.0	0.0	13.262075752820698	0.0
37	0.0015234659459657097	0.0	0.0	13.563264970338118	0.0
38	0.0016758125405622807	0.0	0.0	13.890505455531553	0.0
39	0.0016758125405622807	0.0	0.0	14.188495394562445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	30	2.1640735E-6	45.000004	11
GTACCGG	20	7.031073E-4	45.0	2
ACCGGAT	20	7.031073E-4	45.0	43
TTTCGCG	20	7.031073E-4	45.0	23
TCGGCGA	20	7.031073E-4	45.0	13
CATTAGA	25	3.888971E-5	45.0	22
CGTTAAG	20	7.031073E-4	45.0	2
GCCGATA	35	1.2108649E-7	45.0	9
TTGCGAA	20	7.031073E-4	45.0	28
GCGATAT	20	7.031073E-4	45.0	9
CCGACAG	20	7.031073E-4	45.0	15
CTCGAGG	50	2.1827873E-11	45.0	2
GTACGAG	35	1.2108649E-7	45.0	1
ACGCATT	120	0.0	43.125004	17
AGTACGG	75	0.0	42.0	2
TACGCAT	125	0.0	41.399998	16
GCTACGA	55	6.002665E-11	40.909092	10
GGCGATA	160	0.0	40.78125	8
TTAGGCG	50	1.0804797E-9	40.5	1
ATAGCGG	100	0.0	40.5	2
>>END_MODULE