FastQCFastQC Report
Sat 18 Jun 2016
SRR3553689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348983
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34620.992025399518028No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17350.4971588873956611No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC13080.3748033571835877No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC11410.3269500233535731No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.23840702842258793No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC6640.1902671476834115No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC6070.17393397386119094No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT5270.1510102211282498Illumina Single End Adapter 1 (95% over 21bp)
GCCAACGGGCAATTTAAATATTTAAACTTTCTAAGCTTATGCCCCCTTGGC5210.14929093967327922No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.13324431276022042No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG4510.12923265603195572No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT4460.12779992148614688No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3840.1100340131181175No Hit
AAAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC3720.10659545020817633No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3570.10229724657074986No Hit
GGGCAAGGGAGAAACAAGCGAGACAGGAATGTCTTACACGCGGGGCAAGAC3550.10172415275242633No Hit
AGTAATGGCGACCTGGGAATCGTTTTGTCTTTCTTCTCTCGGCACTGAAGT3510.1005779651157793No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC3490.10000487129745575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAT502.1827873E-1145.00000425
ATTACTC253.8852028E-545.00000439
TCTAGCC253.8852028E-545.00000439
ATCGTGA302.1611413E-645.00000430
TGCATAG253.8852028E-545.0000041
TGTCTAT253.8852028E-545.00000415
TCATGCA502.1827873E-1145.00000424
CGAATAT253.8852028E-545.00000414
ATGGTAC253.8852028E-545.00000412
TTAGTAT253.8852028E-545.00000416
GTAAGTA302.1611413E-645.0000049
AGCTACG253.8852028E-545.0000049
CCACGAA302.1611413E-645.00000414
ATGCATA502.1827873E-1145.00000426
TATGTAG253.8852028E-545.0000041
CTGATAT253.8852028E-545.00000419
ACGAAAT253.8852028E-545.00000418
GCATAAA207.0265285E-445.032
ACACGCA207.0265285E-445.029
ACGCCTC207.0265285E-445.045
Produced by FastQC (version 0.11.5)