##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553689_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348983
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06848471128966	33.0	31.0	34.0	30.0	34.0
2	32.20282649871197	34.0	31.0	34.0	30.0	34.0
3	32.23471343876349	34.0	31.0	34.0	30.0	34.0
4	35.79145975591934	37.0	35.0	37.0	35.0	37.0
5	35.72293779353149	37.0	35.0	37.0	35.0	37.0
6	35.68792176123192	37.0	35.0	37.0	33.0	37.0
7	35.74506208038787	37.0	35.0	37.0	35.0	37.0
8	35.60007794075929	37.0	35.0	37.0	35.0	37.0
9	37.47702037061977	39.0	37.0	39.0	35.0	39.0
10	37.01090024442451	39.0	37.0	39.0	33.0	39.0
11	36.95030990048226	39.0	37.0	39.0	33.0	39.0
12	36.803592152053255	39.0	35.0	39.0	33.0	39.0
13	36.63451801377145	39.0	35.0	39.0	33.0	39.0
14	37.75405678786646	40.0	36.0	41.0	33.0	41.0
15	37.88494855050246	40.0	36.0	41.0	33.0	41.0
16	37.99602272890083	40.0	36.0	41.0	33.0	41.0
17	37.93787663009373	40.0	36.0	41.0	33.0	41.0
18	37.84681202236212	39.0	36.0	41.0	33.0	41.0
19	37.694927833160925	39.0	36.0	41.0	33.0	41.0
20	37.597676677660516	39.0	35.0	41.0	33.0	41.0
21	37.441904046901996	39.0	35.0	41.0	33.0	41.0
22	37.41277941905479	39.0	35.0	41.0	33.0	41.0
23	37.277512085115895	39.0	35.0	41.0	33.0	41.0
24	37.26550576962202	39.0	35.0	41.0	33.0	41.0
25	37.197023923801446	39.0	35.0	41.0	33.0	41.0
26	37.086949794116045	39.0	35.0	41.0	32.0	41.0
27	37.05556717662465	39.0	35.0	41.0	32.0	41.0
28	36.99382491410756	39.0	35.0	41.0	32.0	41.0
29	36.896189785748874	39.0	35.0	41.0	32.0	41.0
30	36.73190384631916	39.0	35.0	41.0	31.0	41.0
31	36.57739775289914	39.0	35.0	41.0	31.0	41.0
32	36.37167999587373	38.0	35.0	41.0	31.0	41.0
33	36.23095394331529	38.0	35.0	40.0	30.0	41.0
34	36.125430178547376	38.0	35.0	40.0	30.0	41.0
35	35.958702859451606	38.0	35.0	40.0	30.0	41.0
36	35.803299874205905	38.0	35.0	40.0	29.0	41.0
37	35.70873652871343	38.0	35.0	40.0	29.0	41.0
38	35.58810027995633	38.0	35.0	40.0	28.0	41.0
39	35.490298954390326	38.0	35.0	40.0	27.0	41.0
40	35.36155629357304	38.0	35.0	40.0	27.0	41.0
41	35.13820443975781	38.0	34.0	40.0	26.0	41.0
42	35.02414157709688	38.0	34.0	40.0	26.0	41.0
43	34.89926729955327	37.0	34.0	40.0	25.0	41.0
44	34.77264795133287	37.0	34.0	40.0	24.0	41.0
45	34.66596940252104	37.0	34.0	40.0	24.0	41.0
46	34.528166128436055	37.0	34.0	40.0	23.0	41.0
47	34.40663871879146	37.0	33.0	40.0	23.0	41.0
48	34.25309828845531	37.0	33.0	40.0	23.0	41.0
49	34.199038348572856	36.0	33.0	40.0	23.0	41.0
50	34.071295736468535	36.0	33.0	40.0	23.0	41.0
51	32.65646750701323	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	7.0
13	1.0
14	5.0
15	26.0
16	58.0
17	101.0
18	215.0
19	387.0
20	682.0
21	1024.0
22	1518.0
23	2213.0
24	2849.0
25	3487.0
26	4058.0
27	4487.0
28	5143.0
29	6093.0
30	7112.0
31	8979.0
32	10962.0
33	15261.0
34	26653.0
35	35890.0
36	28419.0
37	39787.0
38	62931.0
39	80612.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93012267073181	21.97929412034397	23.077341876251854	13.013241332672365
2	30.64074754357662	28.080164363307098	25.137327606215777	16.14176048690051
3	29.92867847430964	29.383666253083963	25.286331998979893	15.401323273626508
4	26.61161145385305	29.831539072103798	25.125579182940143	18.431270291103004
5	23.102271457348923	33.83889759673107	23.879959768813954	19.17887117710605
6	22.65296590378328	38.66979193828926	25.24564233787892	13.43159982004854
7	77.21722834636644	10.638627096448824	8.039073536533298	4.105071020651436
8	78.34708280919128	8.962327677852503	7.9066888645005635	4.783900648455655
9	72.69838358888542	12.119788069905985	9.242570555012708	5.939257786195889
10	38.060306662502185	28.115696180043155	17.783387729488258	16.040609427966405
11	32.19354524432422	29.775662424817256	20.689546482206868	17.341245848651653
12	27.338007868578124	26.543986383290875	27.812243003240845	18.305762744890153
13	23.550717370187087	31.46170443832508	27.39875581332042	17.588822378167418
14	19.697807629598003	33.19989798930034	27.903078373445123	19.199216007656535
15	18.40777344455174	29.80718258482505	33.4377319239046	18.34731204671861
16	19.29635540986237	26.848872294638994	33.79763484181178	20.057137453686856
17	20.478934503972972	28.666439339452065	25.842519549662878	25.01210660691208
18	20.76089666258815	27.56466647372508	29.889135000845314	21.785301862841454
19	23.837550826258013	30.18914961473768	24.698624288289114	21.274675270715193
20	25.102082336388882	28.839227125676608	25.481470444119054	20.577220093815455
21	22.36527280698487	31.561709309622533	25.93536074823129	20.13765713516131
22	22.286758953874543	27.83488020906463	25.445365533564672	24.432995303496156
23	19.733625993243223	30.96511864474774	25.700678829627805	23.600576532381233
24	20.42936188868799	28.862723972227872	28.594516065252463	22.11339807383168
25	20.122470148975736	30.84992678726471	26.504729456735713	22.52287360702384
26	18.831289776292827	32.244837141064174	26.528799397105303	22.395073685537692
27	20.68754065384274	31.772894381674753	25.85741998893929	21.68214497554322
28	19.175719161105267	30.950504752380485	29.093967327921415	20.779808758592825
29	20.59326672072852	31.075725751684182	26.006997475521732	22.324010052065574
30	21.426258585661767	31.362845754664264	26.497852330915833	20.713043328758136
31	23.745569268417086	30.327551771862815	24.25934787654413	21.667531083175973
32	24.285996739096174	31.11698850660347	24.482854465690306	20.114160288610048
33	23.665336133851792	31.126444554605815	22.55869197066906	22.649527340873338
34	20.989274549190075	31.017843276033503	25.34879922517716	22.644082949599266
35	22.754403509626545	29.980830011777076	24.084840808864616	23.179925669731762
36	24.343306120928528	31.699824919838505	23.510600802904438	20.446268156328532
37	22.287905141511192	31.343074017932103	26.116458394821525	20.25256244573518
38	23.30113501230719	29.86879017029483	24.392019095486024	22.438055721911958
39	24.26421917399988	28.24808085207589	24.897201296338217	22.590498677586016
40	25.420435952467596	27.234277887461566	25.52445248049332	21.820833679577515
41	21.56494728969606	29.38166042471983	25.180023095680877	23.873369189903233
42	22.171280549482354	28.96559431261695	26.642271973133365	22.220853164767338
43	24.06420943140497	27.709659209760932	26.060295200625816	22.16583615820828
44	22.050357753816087	27.65435565629271	25.94538989005195	24.349896699839245
45	23.838123920076338	27.462369227154333	25.37974629136663	23.319760561402706
46	23.84299521753209	28.37645386738036	25.51098477576272	22.26956613932484
47	21.96611296252253	28.60683758234642	26.24884306685426	23.17820638827679
48	22.420576360453087	28.0936320680377	27.16034878489783	22.325442786611383
49	23.569629466191763	26.40787660143904	27.219090901275994	22.803403031093204
50	22.421436001180574	27.02968339432007	27.274107907835056	23.274772696664307
51	22.27644326514472	26.97810495067095	24.323247837287205	26.422203946897127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4459.0
1	4056.5
2	3654.0
3	3250.0
4	2846.0
5	2218.5
6	1591.0
7	1859.0
8	2127.0
9	2265.5
10	2404.0
11	2272.0
12	2140.0
13	2156.0
14	2172.0
15	2131.0
16	2090.0
17	2056.5
18	2023.0
19	2050.5
20	2078.0
21	2031.5
22	1985.0
23	1971.0
24	1957.0
25	2484.5
26	3254.5
27	3497.0
28	4090.0
29	4683.0
30	4516.5
31	4350.0
32	4804.5
33	5259.0
34	6425.5
35	7592.0
36	7709.5
37	7827.0
38	8961.0
39	10095.0
40	12215.5
41	14336.0
42	16364.5
43	18393.0
44	20942.0
45	23491.0
46	25315.5
47	27140.0
48	29092.0
49	31044.0
50	31444.0
51	31844.0
52	26969.0
53	22094.0
54	20397.0
55	18700.0
56	17202.0
57	15704.0
58	14175.5
59	12647.0
60	12115.0
61	11583.0
62	11128.0
63	10673.0
64	9176.5
65	7680.0
66	7190.0
67	6700.0
68	5575.0
69	4450.0
70	4138.5
71	3827.0
72	3910.0
73	3993.0
74	3297.5
75	2185.0
76	1768.0
77	1393.0
78	1018.0
79	877.0
80	736.0
81	539.0
82	342.0
83	235.0
84	128.0
85	112.5
86	97.0
87	69.5
88	42.0
89	48.5
90	55.0
91	50.0
92	45.0
93	24.5
94	4.0
95	5.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	348983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.14708081710827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18580560883814	22.20593833197306
2	9.782161034200758	5.7024287645963385
3	3.393496490844883	2.967315494136874
4	1.7522907301096993	2.0429663810230823
5	1.0415610647781155	1.5179232265520541
6	0.7216950822680223	1.2621182932905388
7	0.5347740192541505	1.0910971090663377
8	0.44081372227215077	1.0278746550685358
9	0.34585385002493063	0.9072567105826226
>10	4.654033311222886	32.51615043005969
>50	1.0085737996184323	19.591092802971275
>100	0.12994508815698336	6.022156778213587
>500	0.004997888006037822	0.9180365751403489
>1k	0.003998310404830258	2.227644447325645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3462	0.992025399518028	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1735	0.4971588873956611	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1308	0.3748033571835877	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	1141	0.3269500233535731	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	832	0.23840702842258793	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	664	0.1902671476834115	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	607	0.17393397386119094	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	527	0.1510102211282498	Illumina Single End Adapter 1 (95% over 21bp)
GCCAACGGGCAATTTAAATATTTAAACTTTCTAAGCTTATGCCCCCTTGGC	521	0.14929093967327922	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	465	0.13324431276022042	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	451	0.12923265603195572	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	446	0.12779992148614688	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	384	0.1100340131181175	No Hit
AAAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	372	0.10659545020817633	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	357	0.10229724657074986	No Hit
GGGCAAGGGAGAAACAAGCGAGACAGGAATGTCTTACACGCGGGGCAAGAC	355	0.10172415275242633	No Hit
AGTAATGGCGACCTGGGAATCGTTTTGTCTTTCTTCTCTCGGCACTGAAGT	351	0.1005779651157793	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	349	0.10000487129745575	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.730938183235286E-4	0.0	0.0	0.16275864440388213	0.0
2	5.730938183235286E-4	0.0	0.0	0.7226713049059696	0.0
3	5.730938183235286E-4	0.0	0.0	0.9696747406034105	0.0
4	5.730938183235286E-4	0.0	0.0	1.3198350635990865	0.0
5	5.730938183235286E-4	0.0	0.0	2.447683698059791	0.0
6	5.730938183235286E-4	0.0	0.0	3.2205007120690694	0.0
7	5.730938183235286E-4	0.0	0.0	3.921394451878745	0.0
8	5.730938183235286E-4	0.0	0.0	5.0128516288759055	0.0
9	5.730938183235286E-4	0.0	0.0	5.495683170813478	0.0
10	5.730938183235286E-4	0.0	0.0	6.6848528438348	0.0
11	5.730938183235286E-4	0.0	0.0	8.209568947484549	0.0
12	5.730938183235286E-4	0.0	0.0	9.310195625574885	0.0
13	5.730938183235286E-4	0.0	0.0	9.705916907127282	0.0
14	5.730938183235286E-4	0.0	0.0	9.866956270076193	0.0
15	5.730938183235286E-4	0.0	0.0	10.164965055604428	0.0
16	5.730938183235286E-4	0.0	0.0	10.834052088497147	0.0
17	5.730938183235286E-4	0.0	0.0	11.578500958499411	0.0
18	5.730938183235286E-4	0.0	0.0	12.541298573282939	0.0
19	5.730938183235286E-4	0.0	0.0	13.005504566124998	0.0
20	5.730938183235286E-4	0.0	0.0	13.404664410587335	0.0
21	5.730938183235286E-4	0.0	0.0	13.879472639068378	0.0
22	8.59640727485293E-4	0.0	0.0	14.328205098815701	0.0
23	8.59640727485293E-4	0.0	0.0	14.840264425487774	0.0
24	8.59640727485293E-4	0.0	0.0	15.218506345581304	0.0
25	8.59640727485293E-4	0.0	0.0	15.58643257694501	0.0
26	0.0011461876366470572	0.0	0.0	15.903926552296245	0.0
27	0.0011461876366470572	0.0	0.0	16.225718731284903	0.0
28	0.0011461876366470572	0.0	0.0	16.536049033907094	0.0
29	0.0011461876366470572	0.0	0.0	16.897957780178405	0.0
30	0.0011461876366470572	0.0	0.0	17.29826381227739	0.0
31	0.0011461876366470572	0.0	0.0	17.646991400727256	0.0
32	0.0011461876366470572	0.0	0.0	18.002023021178683	0.0
33	0.0011461876366470572	0.0	0.0	18.348458234355256	0.0
34	0.0011461876366470572	0.0	0.0	18.681425742801224	0.0
35	0.0011461876366470572	0.0	0.0	19.05938111598559	0.0
36	0.0011461876366470572	0.0	0.0	19.400945031706417	0.0
37	0.0011461876366470572	0.0	0.0	19.75912866815862	0.0
38	0.0011461876366470572	0.0	0.0	20.078628471873987	0.0
39	0.0011461876366470572	0.0	0.0	20.41818655923068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCAT	50	2.1827873E-11	45.000004	25
ATTACTC	25	3.8852028E-5	45.000004	39
TCTAGCC	25	3.8852028E-5	45.000004	39
ATCGTGA	30	2.1611413E-6	45.000004	30
TGCATAG	25	3.8852028E-5	45.000004	1
TGTCTAT	25	3.8852028E-5	45.000004	15
TCATGCA	50	2.1827873E-11	45.000004	24
CGAATAT	25	3.8852028E-5	45.000004	14
ATGGTAC	25	3.8852028E-5	45.000004	12
TTAGTAT	25	3.8852028E-5	45.000004	16
GTAAGTA	30	2.1611413E-6	45.000004	9
AGCTACG	25	3.8852028E-5	45.000004	9
CCACGAA	30	2.1611413E-6	45.000004	14
ATGCATA	50	2.1827873E-11	45.000004	26
TATGTAG	25	3.8852028E-5	45.000004	1
CTGATAT	25	3.8852028E-5	45.000004	19
ACGAAAT	25	3.8852028E-5	45.000004	18
GCATAAA	20	7.0265285E-4	45.0	32
ACACGCA	20	7.0265285E-4	45.0	29
ACGCCTC	20	7.0265285E-4	45.0	45
>>END_MODULE